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Antioxidant and Immunomodulatory Effects of Ulva pertusa kjellman on Broiler Chickens (육계에서 구멍갈파래의 항산화 및 면역조절 효과)

  • Hong, Joon-Ki;Bong, Mi-Hee;Park, Jun-Cheol;Moon, Hong-Kil;Kim, Dong-Wook;Lee, Sang-Cheul;Lee, Jun-Heon
    • Journal of Animal Science and Technology
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    • v.53 no.5
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    • pp.419-428
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    • 2011
  • This study was conducted to test the efficacy of Ulva pertusa kjellman as immunomodulators under lipopolysaccharide (LPS) challenge in broilers by investigating their effects on serum superoxide dismutase (SOD) like ability, immunoglobulin concentration, and splenic cytokine mRNA expression. A total of ninety six1-d-old male broiler chicks (Ross 308) were divided into 4 treatment groups with 8 replicates of 3 birds in each group. NC (negative control, no immune substances), PC (positive control, ${\beta}$-glucan 25 ppm), Ulva P (Ulva pertusa kjellman Powder 3%), and Ulva E (Extract form Ulva pertusa kjellman 0.3%) were added in feed with respective substance. Heparinized venous blood and spleens were collected from five birds per dietary treatment at 5 wk of age. The SOD like activities in Ulva P and Ulva E were significantly increased in comparison with other groups (P<0.05). The immunoglobulin M content was lower in the Ulva E than other groups (P<0.05). Expression patterns of splenic cytokine mRNA in the IL-$1{\beeta}$, IL-2 and IL-6 were similar. Expression rate of IL-$1{\beta}$, IL-2 and IL-6 in Ulva pertusa kjellman (Ulva P, Ulva E) were decreased (P<0.05) in comparison to other groups. Expression rate of IL-$1{\beta}$, IL-2 and IL-6 were significantly lower in groups treated with Ulva E than Ulva P. In conclusion, dietary supplementation of Ulva pertusa kjellman improved SOD like activity and affect immune system by inhibiting inflammatory response in broiler chicks. In addition, it is more effective to use Ulva pertusa kjellman extract than powder for immunomodulatory function. These results suggest the possibility that Ulva pertusa kjellman could be used as the immunomodulator for inflammatory response in broiler chicks.

Location Analysis of A Collective Consumption Facility with GIS: The Location of Gu-Office and Its Administrative District in Ulsan, South Korea (GIS기법을 이용한 도시공공서비스 시설의 입지분석 -울산시 구 관할구역과 구청입지를 중심으로-)

  • Cho, Sung-Ho;Park, Soon-Ho
    • Journal of the Korean association of regional geographers
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    • v.2 no.1
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    • pp.69-85
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    • 1996
  • This research adoptes a spatial analysis function of the Geographic Information System[GIS] to analyze the location and the service district of a collective consumption facility, to discover the optimal location, and to redefine the service district. The location and spatial district of a collective consumption facility strongly influences the quality of life of residents. This research studies administrative offices and districts, especially Gu-offices in Ulsan. Ulsan would be raised to the status of Kwangyokshi in 1997; so that, the status of four Gus would be changed from a general administration into a self-governing administration. The summary of this research focuses on five significant points. First, the districts of four Gus were strongly required to be redefined to obtain the maximization of the degree of potential development as well as of the efficiency and equity of administring services. The spatial range of residents in a Gu was not in accord with its administrative district. The administrative districts of four Gus in Ulsan were not balanced in terms of area and population, and the high degree of disparity among Gus existed the efficiency and equality of a collective consumption service. Second, the current gu-offices were located based on security of land and accessibility of a main route; so, there was difficult to find the creteria and the principles of selections of the location of Gu offices. The social disparity of an administrative service existed in the accessibility into Gu-offices. Third, the administrative districts of Gus were redefined with spatial analysis tool of ARC/INFO. It was recommended that Ulsan maintain four Gus under the condition of five Kwangyokshi in South Korea. The redefined districts of administration reduced the disparity among four Gus in terms of area and population. improved the degree of harmony between the spatial range of residents of Gus and the administrative district of Gus, and increased the efficiency and equity of administrative service. Fourth, within the redefined adminis trative district of a Gu, the centroid reduced the maximum distance and mean distance; so, the efficiency and equity of public service provided by the Gu-office were improved. Last, the spatial analysis function of GIS helped to select the optimal location and to delineate the district of public service with more speedly and objectively. The function of spatial analysis of GIS was very useful to minimize the conflict in the determination of the location of a collective consumption facility and of the service district. To improve location analysis with GIS. non-spatial data base such as budget, thought of residents, and development policy and program, should be constructed.

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Bakanae Disease Reduction Effect by Use of Silicate Coated Seed in Wet Direct-Seeded Rice (규산코팅 벼 종자를 이용한 담수직파재배 시 벼 키다리병 경감효과)

  • Kang, Yang-Soon;Kim, Wan Joong;Kim, Yeon Ju;Jung, Ki-Hong;Choi, Ul-Su
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.61 no.1
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    • pp.9-16
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    • 2016
  • To investigate the effect of soluble silicate zeolite dressing of the rice against bakanae disease, field trial in reclaimed land and in vitro were carried out. The coated rice seeds (SCS) which were dressed with the mixture of 25% silicic acids (binder), and the zeolite (coating powder). In wet direct seeding, uniform scattering of rice seeds on the soil surface and the better seedling establishment were shown in SCS treatment plots. The incidence of bakanae disease began from the mid tillering stage toward the heading stage. Around heading stage, the ratio of infected tillers reached its highest point by 9.9% in non-SCS treatment plots. While, in SCS treatment plots, the ratio of infected tillers was no more than 0.01%. The vitality of the pathogenic fungi of bakanae disease in the SCS and non-SCS samples were assessed. Samples were incubated for one week keeping proper humidity at $30^{\circ}C$ after inoculated with panicles of infected rice plants from experimental field plots. In non-SCS treatment, pinkish colonies were formed on the grain surface of panicle of infected plants, and mycelium, macro-conidia and micro-conidia were developed actively inside part of infected grain inoculated. While in SCS treatment, micro-conidia and mycelium were not survived and the growth of macro-conidia, mycelia were greatly inhibited and withered. Based on the results, it is concluded that the environmental friendly control of bakanae disease by use of SCS is possible and soluble silicate can be applied as agents for replacement of seed disinfection.

Effect of Barley Green Manure on Rice Growth and Yield According to Tillage Date in Spring (녹비보리의 환원시기가 후작물 벼 생육 및 수량에 미치는 영향)

  • Kim, Min-Tae;Ku, Ja-Hwan;Jeon, Weon-Tai;Seong, Ki-Yeong;Park, Chang-Young;Ryu, Jin-Hee;Cho, Hyeoun-Suk;Oh, In-Seok;Lee, Yong-Hwan;Lee, Jong-Ki;Park, Man;Kang, Ui-Gum
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.2
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    • pp.119-123
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    • 2011
  • To use barley as a green manure crop, this study has been conducted in Suwon, Gyeonggi-do to establish the barley green manure (BGM)-rice cropping system with emphasis on soil incorporation date and BGM density. The nitrogen (N) contents of rye and barley, grass green manure crops, were 1.4~2.4% at early growth stage and decreased rapidly to 0.6~1.0% at late growth stage. The biomass of barley was 449 kg/l0a at heading stage (HS) and increased to 421 kg/10a at 10 days after heading stage (DAH), 473 kg/10a at 20 DAH. C/N ratio of BGM was the lowest 26.3 at HS. The N contents of BGM was in the range of 0.9~1.5%, the highest at HS and gradually decreased, and the output of N were 4.3-6.3 kg/10a. The total amount of nitrogen, phosphorous and potassium of BGM showed the highest level at 10 DAH. Culm length of rice was relatively longer as the BGM application time was delayed. The application of BGM into soil increased plant height of rice by 7.2~7.7 em as compared to the plants treated with commercial fertilizer at recommended rate. but panicle length of rice showed a similar tendency in both the soil-applied of BGM and commercial fertilizer. N contents of unhulled rice was the highest at HS of BGM and followed by 10 DAH of BGM and 20 DAH of BGM. This trend could also be seen in rice straw. The yield of rice in the soil-applied of BGM was 10~15% lower than in the soil-applied of commercial fertilizer. Based on this study, application of BGM made it possible to save 30~50% of application amount of nitrogen fertilizer for following crops.

Seedling Establishment, Overwintering Ability and Dry Matter Production of Chinese Milk Vetch (Astragalus sinicus L.) in Natural Reseeding Practices (자운영 지속재배시 입모, 월동 및 생육 특성)

  • Kim, Sang-Yeol;Oh, Seong-Hwan;Lee, Jong-Hee;Cho, Jun-Hyeon;Han, Sang-Ik;Jeong, Jin-Il;Jeong, Kuk-Hyun;Choi, Kyung-Jin;Park, Sung-Tae;Kim, Jeong-Il;Lee, Ji-Yoon;Song, You-Cheon;Yeo, Un-Sang;Kang, Hang-Won
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.1
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    • pp.8-13
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    • 2010
  • Seedling establishment, overwintering ability and dry matter production of Chinese milk vetch (Astragalus sinicus L. CMV) in natural reseeding practice were compared with the annual seedling in 2006~2007 and 2007~2008. In natural reseeding, CMV seeds were distributed up to 15 cm soil depth in autumn after rice harvest and the seeds generally emerged from soil at less than 5 cm depth while they all emerged from soil surface in the annual seeding. It took 7-8 d longer in seedling establishment of the natural reseeding practice than the annual seeding. Generally, the natural reseeding practice showed higher seedling stand than the annual seeding because of high seedling survival rate. The high seedling survival rate in natural reseeded plant was attributed to the longer root length than that of the annual-seeded plant. In annual seeding, most of the seeds emerged from the soil surface and the root can not easily penetrate the soil while, in natural reseeding technology, the seeds incorporated into the soil during land preparation emerged from the soil at 0 to 5 cm depths, thereby having longer root length. The long root length contributed to greater ability to survive even under low temperature and low soil moisture conditions during winter. The dry matter production in the natural reseeding practice was also higher than the annual seeding when the temperature is low and soil moisture is not sufficient. This result indicates that natural reseeding technology is more stable and beneficial in seedling establishment and dry matter production as compared with the annual seeding especially under unfavorable environmental condition for CMV growth. This practice could be encouraged in CMV-rice cropping system in the southern parts of Korea.

Effects of Hydrogen Peroxide on Germination and Early Growth of Sorghum (Sorghum bicolor) (과산화수소 처리가 수수의 발아 및 초기 생장에 미치는 효과)

  • Shim, Doobo;Song, Ki Eun;Park, Chan Young;Jeon, Seung Ho;Hwang, Jung Gyu;Kang, Eun-ju;Kim, Jong Cheol;Shim, Sangin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.2
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    • pp.140-148
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    • 2018
  • As the global warming causing desertification increase, there is growing concern about damage of crops. It was to investigate how the treatment with hydrogen peroxide before leaf development affects the growth and yield of sorghum for minimizing a damage of crops to drought. The germination experiment was conducted at alternating temperature of $25^{\circ}C/20^{\circ}C$(12 hr/12 hr) under water stress condition of 0 ~ -0.20 MPa adjusted with PEG solution containing 0 and 10 mM $H_2O_2$. In order to know the effect of foliar application of hydrogen peroxide on the growth of sorghum, 10 mM hydrogen peroxide was treated to leaves at 3-leaf stage of sorghum growing in greenhouse conditions. Seed germination rate was increased by 20% in hydrogen peroxide treatment as compared to the Control. under water stress conditions (-0.15 ~ -0.20 MPa). The length of seedlings was also on the rise by the hydrogen peroxide treatment. In the greenhouse pot experiment, the morphological characteristics (plant height, stem diameter, leaf length, and leaf number) and physiological characteristics (chlorophyll content, chlorophyll fluorescence (Fv/Fm), stomatal conductance) were higher in the plants treated with hydrogen peroxide under the drought stress condition than those of plants of $H_2O$ treatment. Experiment conducted with the soil moisture gradient system showed that the foliar application of hydrogen peroxide increased photosynthetic ability of sorghum plant with respect to SPAD value and stomatal conductance and rooting capacity (root weight and root length) under drought condition. Generally, hydrogen peroxide treatment in sorghum increased the tolerance to drought stress and maintained better growth due to ameliorating oxidative stress.

Assessment of Adsorption Capacity of Mushroom Compost in AMD Treatment Systems (광산배수 자연정화시설 내 버섯퇴비의 중금속 흡착능력 평가)

  • Yong, Bo-Young;Cho, Dong-Wan;Jeong, Jin-Woong;Lim, Gil-Jae;Ji, Sang-Woo;Ahn, Joo-Sung;Song, Ho-Cheol
    • Economic and Environmental Geology
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    • v.43 no.1
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    • pp.13-20
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    • 2010
  • Acid mine drainage (AMD) from abandoned mine sites typically has low pH and contains high level of various heavy metals, aggravating ground- and surface water qualities and neighboring environments. This study investigated removal of heavy metals in a biological treatment system, mainly focusing on the removal by adsorption on a substrate material. Bench-scale batch experiments were performed with a mushroom compost to evaluate the adsorption characteristics of heavy metals leached out from a mine tailing sample and the role of SRB in the overall removal process. In addition, adsorption experiments were perform using an artificial AMD sample containing $Cd^{2+}$, $Cu^{2+}$, $Pb^{2+}$ and $Zn^{2+}$ to assess adsorption capacity of the mushroom compost. The results indicated Mn leached out from mine tailing was not subject to microbial stabilization or adsorption onto mushroom compost while microbially mediated stabilization played an important role in the removal of Zn. Fe leaching significantly increased in the presence of microbes as compared to autoclaved samples, and this was attributed to dissolution of Fe minerals in the mine tailing in a response to the depletion of $Fe^{3+}$ by iron reduction bacteria. Measurement of oxidation reduction potential (ORP) and pH indicated the reactive mixture maintained reducing condition and moderate pH during the reaction. The results of the adsorption experiments involving artificial AMD sample indicated adsorption removal efficiency was greater than 90% at pH 6 condition, but it decreased at pH 3 condition.

Preliminary Study of Oxidized Au skarn Model in the Geodo Mine Area to Mineral Exploration (광물자원탐사를 위한 거도광산지역의 산화형 스카른 금광상모델 예비연구)

  • Kim, Eui-Jun;Park, Maeng-Eon;Sung, Kyul-Youl
    • Economic and Environmental Geology
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    • v.42 no.4
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    • pp.289-300
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    • 2009
  • The Geodo mine area, had been developed for Fe and Cu ores since 1963 and abandoned in recent decades, is located in the central part of the Taebaeksan mineralized district. This area comprises of the Jangsan, Myobong, Pungchon, Hwajeol, Dongjeom, and Dumugol Formations in ascending stratigraphic order. These Formations were intruded by the Cretaceous Eopyeong granitoids that appears to produce the Geodo skarn. Their compositions are relatively oxidized quartz monzodiorite to granodiorite (magnetite series, $Fe_2O_3/FeO=0.3{\sim}1.1$). Mineralizations related skarn deposit occur in the Myobong, Pungchon, and Hwajeol Formations. The proximal skarn is zoned from andraditic garnet ($Ad_{44-95}Gr_{1-53}$) predominant adjacent to the Eopyeong granitoids to diopsidic pyroxene ($Hd_{10-100}Di_{0-89}$) predominant away from the one. The differential proportion of garnet and pyroxene is generated by water/rock ratio and their source, such as magmatic and meteoric water. This is useful tool for assessment the overall oxidation state of the entire skarn system. Gold occurs in proximal red to brownish garnet skarn, and genetically associated with Bi- and Te-bearing minerals. Skarn deposit developed in the Geodo mine area is considered as oxidized Au skarn category, based on chemical composition of the Eopyeong granitoids, zonation of skarn, and gold occurrences. Garnet-rich skarn zone will be the main target for exploration of gold in the study area. However, it is needed to the detailed survey on vertical zonation of this area as well as lateral zonation. The result of this survey would provide an important basis for the exploration of the skarn Au deposit in the Geodo mine area.

Changes of Soil Properties through the Remediation Processes and Techniques for the Restoration of Remediated Soils (오염 토양 정화공정에 의한 토양의 특성 변화 및 정화토의 회복기술)

  • Lee, Sang-Woo;Lee, Woo-Chun;Lee, Sang-Hun;Kim, Soon-Oh
    • Economic and Environmental Geology
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    • v.53 no.4
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    • pp.441-477
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    • 2020
  • There have been raised other environmental issues related to remediated soils piled up in numerous carry-out processing facilities because a considerable quantity of them have been produced every year, but most of them have not been relevantly reused or recycled. Thus, this article reports the trend of researches on the development of techniques to restore the quality of remediated soils to activate their reuse and recycling. Firstly, the tendency of change in soil properties through remediation processes was looked over, and then the degradation of soil quality was characterized according to the type of remediation processes. Besides, the direction of policy to promote the reuse and recycling of remediated soils was introduced, and finally, the future works needed were suggested. This article was prepared based on the results of the survey of domestic and foreign literature. A number of literature were reviewed to scrutinize the change of soil properties due to remediation processes and diverse techniques for the amendment and restoration of remediated soils. Furthermore, the policies related to the reuse and recycling of remediated soils were arranged with the reference of the first and second versions of the Soil Conservation Master Plan of Korea. The literature survey focused on three kinds of remediation technologies, such as land farming, soil washing, and thermal desorption, which were most frequently used so far in Korea. The results indicate that the tendency of change in soil properties was significantly different depending on the type of remediation processes applied, and the degradation characteristics of soil quality were also totally different between them. The soil amendment and restoration can be categorized as three techniques depending on the type of substances used, such as inorganic, organic, and biological ones. Diverse individual materials have been used, and the soil properties improved or enhanced were dependent on the type of specific materials utilized. However, few studies on the restoration of soil qualities degraded during the remediation processes have not been carried out so far. The second Soil Conservation Master Plan states the quality certification and target management system of remediated soils, and it is expected that their reuse and recycling will be facilitated hereafter. With the consideration of the type of remediation processes implemented and public utility, the restoration technologies of remediated soils should be developed for the vitalization of their reuse and recycling. Besides, practical and specific measures should be taken to support the policy specified in the second Soil Conservation Master Plan and to promote reuse/recycling of remediated soils.

Microbiological Safety Assessment to Secure Safety of Food Service in University (대학 내 급식소의 안전성 확보를 위한 미생물학적 안전성 평가)

  • Kim, Kyeong-Yeol;Nam, Min-Ji;Nam, Bo-Ram;Ryu, Hee-Jung;Heo, Rok-Won;Shim, Won-Bo;Chung, Duck-Hwa
    • Journal of Food Hygiene and Safety
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    • v.25 no.1
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    • pp.49-58
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    • 2010
  • The objectives of this study were to investigate the microbial contamination levels on food service in university and to provide the information of microbial contamination to improve food safety. A total of 288 samples were collected during summer and winter season between 2006 and 2008 from 4 food services located in the university in Western Gyeongnam and were used to detect sanitary indicator bacteria [aerobic plate count (APC), coliform, and Escherichia coli] and pathogenic bacteria (Staphylococcus aureus, Salmonella spp.). As a result, APC and coliform for hand and kitchen utensils which are used often by the employee were detected at high levels of 1.1~5.5 and 1.3~5.3 log CFU/($100\;cm^2$, hand), respectively. The contamination levels of APC and coliform in cooked foods and drinking water were 0.8~6.4 and 1.3~5.0 log CFU/(g, mL), respectively. Especially, the cooked foods showed the highest contamination for APC (2.1~6.4 log CFU/g) and coliform (1.0~5.0 log CFU/g). We think the reason that the cooked foods may be contaminated with APC and coliform on cooking process by using employee's hand and kitchen utensils. Moreover, S. aureus for hand and kitchen utensils was detected at levels of 2.8~3.0 and 2.0~2.3 log CFU/(g, hand), but Salmonella spp. was not detected. According to the above results, contamination levels of the samples were mostly decreased irrespective of summer and winter season. The results obtained indicated that it is necessary to periodic monitoring for microorganism contamination and education about personal and environmental hygiene to employee for ensuring food safety of food service in university.