DOI QR코드

DOI QR Code

Bakanae Disease Reduction Effect by Use of Silicate Coated Seed in Wet Direct-Seeded Rice

규산코팅 벼 종자를 이용한 담수직파재배 시 벼 키다리병 경감효과

  • Kang, Yang-Soon (Saturn Bio Tec Co., Ltd.) ;
  • Kim, Wan Joong (Saturn Bio Tec Co., Ltd.) ;
  • Kim, Yeon Ju (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University) ;
  • Jung, Ki-Hong (Department of Plant Molecular System Biotechnology & Crop Biotech Institute, Kyung Hee University) ;
  • Choi, Ul-Su (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University)
  • 강양순 (새턴바이오텍(주), 수용성규산 작물연구소) ;
  • 김완중 (새턴바이오텍(주), 수용성규산 작물연구소) ;
  • 김연주 (경희대학교, 생명과학대학 한방재료공학과) ;
  • 정기홍 (경희대학교, 생명공학원, 작물 생명공학팀) ;
  • 최을수 (경희대학교, 생명과학대학 한방재료공학과)
  • Received : 2015.03.31
  • Accepted : 2016.03.07
  • Published : 2016.03.31

Abstract

To investigate the effect of soluble silicate zeolite dressing of the rice against bakanae disease, field trial in reclaimed land and in vitro were carried out. The coated rice seeds (SCS) which were dressed with the mixture of 25% silicic acids (binder), and the zeolite (coating powder). In wet direct seeding, uniform scattering of rice seeds on the soil surface and the better seedling establishment were shown in SCS treatment plots. The incidence of bakanae disease began from the mid tillering stage toward the heading stage. Around heading stage, the ratio of infected tillers reached its highest point by 9.9% in non-SCS treatment plots. While, in SCS treatment plots, the ratio of infected tillers was no more than 0.01%. The vitality of the pathogenic fungi of bakanae disease in the SCS and non-SCS samples were assessed. Samples were incubated for one week keeping proper humidity at $30^{\circ}C$ after inoculated with panicles of infected rice plants from experimental field plots. In non-SCS treatment, pinkish colonies were formed on the grain surface of panicle of infected plants, and mycelium, macro-conidia and micro-conidia were developed actively inside part of infected grain inoculated. While in SCS treatment, micro-conidia and mycelium were not survived and the growth of macro-conidia, mycelia were greatly inhibited and withered. Based on the results, it is concluded that the environmental friendly control of bakanae disease by use of SCS is possible and soluble silicate can be applied as agents for replacement of seed disinfection.

벼 직파재배에서 볍씨에 피복재인 지오라이트와 결합재인 수용성규산(25%)으로 혼합 코팅된 "규산코팅볍씨(SCS)"의 활용에 의한 키다리병 억제효과를 구명하고자 서해안 간척지유래토양에서 담수표면직파된 포장시험과 아울러 시험포장에서 발병된 키다리병 이병주를 접종원으로 하여 변온조건(주야 $18{\sim}26^{\circ}C$)과 항온조건($30^{\circ}C$)에서 실내시험으로 수행한 결과는 다음과 같다. 1. 담수직파에서 규산코팅볍씨 처리구는 무코팅볍씨 처리구에서 보다 입모분포상태가 균일하였고 분얼중기경 건물생산량이 13% 증가되었다. 2. 무코팅볍씨 처리구에서는 분얼중기 경부터 키다리병이 발생되어 종자감염을 일으키는 출수기 경에 9.9%의 심한 이병경율을 보인 반면에 규산코팅볍씨 처리구에서는 분얼중기까지 거의 발생이 없다가 출수기 무렵에 0.01%의 극미한 발생으로 99.9%의 방제가를 보였다. 이병주의 줄기마디와 이삭영화 감염부위에서는 전형적인 키다리병균의 소형분생자와 대형분생자 및 균사체 등이 확인되었다. 3. 무코팅볍씨와 규산코팅볍씨를 $30^{\circ}C$ 항온기에 각각 치상하고 시험포장에서 발병된 이병주의 이삭을 2주일간 접종 처리한 결과 무코팅볍씨 처리구의 접종이삭 종자에서는 종자표면에 균총이 형성되었고 종자내에는 소형분생자, 대형분생자 및 균사 발육이 왕성하게 증식된 반면에 규산코팅볍씨 처리구에서는 소형분생자와 균사는 생존하지 못하였고 대형분생자와 균사체 발육은 크게 위축되었다. 이상의 결과로 규산코팅볍씨의 활용으로 직파재배 시 종자소독 없이도 친환경적 키다리병 방제가 가능하며, 향후 종자소독제로의 개발도 가능할 것으로 판단되었다.

Keywords

References

  1. Choi, D. H. 1994. Determination of Critical Early Seeding Date for Seedling Emergence in Dry-Seeded Rice Based on Statistical Analysis of Daily Mean Air Temperature in Korea. Korean J. Crop Sci. 39(5) : 437-443.
  2. Choi, H. W., J. M. Kim, S. K. Hong, W. G. Kim, S. C. Chun, and S. H. Yu. 2009. Mating types and optimum culture conditions for sexual state formation of Fusarium fujikuroi isolates. Mycobiology. 37 : 247-250. https://doi.org/10.4489/MYCO.2009.37.4.247
  3. Dehkaei, F., S. H. Padasht, J. Mansouri, and H. Rouhani. 2004. Effects of Paddy Soil Antagonistic Microorganisms of Guilan on the Causal Agent of Rice Bakanae Disease. Journal of Science and Technology of Agriculture and Natural Resources. 8(1) : 213-222.
  4. Furuka, T. 1970. Problems on the infection and control of rice bakanae disease. Plant prot. Japan. 24 : 141-144.
  5. Garnache, C., J. T. Rosen-Molina, and D. A. Sumner. 2010. Economics of carbon credits from voluntary practices on rice farms in the Sacramento valley. University of california agricultural issues center. AIC Rice Report. p. 33.
  6. Johnkutty, I., G. Mathew, and J. Mathew. 2002. Comparsion between transplanting and direct-seeding methods for crop establishment in rice. Journal of Tropical Agriculture 40 : 65-66.
  7. Kang, Y. S. 1985. The influences of silicon on growth of rice plant. Res. Rept. RDA(P.M & U) 27(1) : 57-72.
  8. Kang, Y. S., W. J. Kim, Y. J. Kim, K. H. Jung, and U. S. Choi. 2014. Control of bakanae disease (Gibberella fujikuroi) by rice seed coated with silicate in wet direct seeding. Advanced crop science research young scientist. Korean J. crop sci. p. 24.
  9. Kim, C. K. 1981. Ecological studies of bakanae disease of rice, caused by Gibberella fujikoroi. Kor. Pl. Prot. 20(3) : 146-151.
  10. Kim, J. M., S. K. Hong, W. K. Kim, Y. K. Lee, S. H. Yu, and H. W. Choi. 2010. Fungicide resistance of Gibberella fujikuroi isolates causing rice Bakanae disease and their progeny isolates. Kor. J. col. 38(1) : 75-79.
  11. Ko, J. C., M. K. Baek, W. J. Kim, J. Y. Shon, K. Y. Ha, H. J. Kang, M. S. Shin, and J. K. Ko. 2011. Analysis of low temperature germination ratio in rice cultivars for breeding of direct seeding variety. Korean J. Intl. Agri. 23(1) : 89-94.
  12. Lee, Y. H., M. J. Lee, H. W. Choi, S. T. Kim, J. W. Park, I. S. Myung, K. S. Park, and S. W. Lee. 2011. Development of in vitro seedling method for selection of resistant rice against bakanae disease. Res. plant disease. 17(3) : 288-294 (In Korean). https://doi.org/10.5423/RPD.2011.17.3.288
  13. Ogawa, K. and S. Takeda. 1990. Population of benomyl-resistant rice bakanae fungus in paddy field. Soc. Ann. Phytopathol. J. 56 : 247-249. https://doi.org/10.3186/jjphytopath.56.247
  14. Park, H. G., H. R. Shin, Y. Lee, S. W. Kim, O. D. Kwon, I. J. Park, and Y. I. Kuk. 2003. Influence of water temperature, soaking period, and chemical dosage on bakanae disease of rice(Giberella fujikuroi) in seed disinfection. Kor. J. of Pesticide Science. 7(3) : 216-222.
  15. Park, S. H., S. Y. Kim, C. D. Hwang, H. P. Moon, and S. Peng. 2001. Rice Seedling Establishment and Early Growth Affected by Seeding Depth of Pre - germinated and Soaked Seeds in Wet Soil. Kor. J. Intl. Agri. 13(3) : 193-198.
  16. Park, W. S., H. W. Choi, S. S. Han, D. B. Shin, H. K. Shim, E. S. Jung, S. W. Lee, C. K. Lim, and Y. H. Lee. 2009. Control of Bakanae disease of rice by seed soaking into the mixed solution of procholraz and fludioxnil. Res. Plant Dis. 15 : 94-100 (In Korean). https://doi.org/10.5423/RPD.2009.15.2.094
  17. Park, W. S., W. H. Ye, S. W. Lee, S. S. Han, J. S. Lee, C. K. Lim, and Y. H. Lee. 2008. Eletronic microcopic study for the influence of soaking in hot water and prochloraz solution on spore and mycelium of fusarium fujikuroi infected in rice seed. Res. Plant Dic. 14(3) : 176-181 (In Korean). https://doi.org/10.5423/RPD.2008.14.3.176
  18. Shon, J. Y., C. K. Lee, J. H. Kim, Y. H. Yoon, W. H. Yang, K. J. Choi, M. G. Choi, H. K. Park, J. C. Ko, Y. G. Kim, C. K. Kim, and W. H. Yang. 2012. Comparisons of Growth, Heading and Grain Filling Characteristics between Wet-hill-seeding and Transplanting in Rice. Kor. J. of Crop Sci. 57(2) : 151-159. https://doi.org/10.7740/kjcs.2012.57.2.151
  19. Sung, C. K. and J. H. Choi. 2004. The disinfective effect of garlic extract against Bakanae disease. Jour. Agri. Sci. Chungnam nat'l. Univ. Korea. 31(1) : 45-52.
  20. Wulff, E. G., J. L. Sorensen, M. Lubeck, K. F. Nielsen, U. Thrane, and J. Torp. 2010. Fusarium spp. associated with rice Bakanae: ecology, genetic diversity, pathogenicity and toxigenicity. Environmental Microbiology 12 : 649-657. https://doi.org/10.1111/j.1462-2920.2009.02105.x

Cited by

  1. 벼 상자육묘에서 규산코팅볍씨의 건묘육성과 벼키다리병 경감효과 vol.62, pp.1, 2016, https://doi.org/10.7740/kjcs.2016.62.1.001
  2. 볍씨의 규산코팅방법에 따른 이용특성과 육묘효과 vol.65, pp.1, 2016, https://doi.org/10.7740/kjcs.2020.65.1.030