• 대한미생물학회지
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• 제35권2호
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• pp.97-108
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• 2000

Two-dimensional gel electrophoresis (2-DE) is an essential tool of proteomics to analyse the entire set of proteins of an organism and its variation between organisms. Helicobacter pylori was tried to identify differences between strains. As the first step, whole H. pylori was lysed using high concentration urea contained lysis buffer [9.5 M Urea, 4% CHAPS, 35 mM Tris, 65 mM DTT, 0.01% SDS and 0.5% Ampholite (Bio-Rad, pH 3-10)]. The extract ($10\;{\mu}g$) was rehydrated to commercially available immobilised pH gradient (IPG) strips, then the proteins were separated according to their charges as the first dimensional separation. The IPG strips were placed on Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) to separate according to molecular mass of the proteins as the second dimension. The separated protein spots were visualised by silver staining in order to compare different expression of proteins between strains. Approximately 120 spots were identified in each mini-protein electrophoresised gel, furthermore about 65 to 75 spots were regarded as identical proteins in terms of pI value and molecular weight between strains used. In addition, distinct differences were found between strains, such as 219-1, Y7 and Y14, CH150. Two representative strains were examined using strips which had pH range from 4 to 7. This strips showed a number of isoforms which were considered large spots on pH range 3-10. Furthermore, the rest of spots on pH 4-7 IPG strips appeared very distinctive compared to broad range IPG strips. 2-DE seems to be an excellent tool for analysing and identifying variations between H. pylori strains.

• Molecules and Cells
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• 제22권1호
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• pp.119-125
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• 2006

The rat is an accepted model for studying human psychiatric/neurological disorders. We provide a protocol for total soluble protein extraction using trichloroacetic acid/acetone (TCA/A) from rat (female) whole brain, 10 brain regions and the pituitary gland, and show that two-dimensional gel electrophoresis (2-DGE) using precast immobilized pH (4-7) gradient (IPG) strip gels (13 cm) in the first dimension yields clean silver nitrate stained protein profiles. Though TCA/A precipitation may not be "ideal", the important choice here is the selection of an appropriate lysis buffer (LB) for solubilizing precipitated proteins. Our results reveal enrichment of protein spots by use of individual brain regions rather than whole brain, as well as the presence of differentially expressed spots in their proteomes. Thus individual brain regions provide improved protein coverage and are better suited for differential protein detection. Moreover, using a phosphoprotein-specific dye, ingel detection of phosphoproteins was demonstrated. Representative high-resolution silver nitrate stained proteome profiles of rat whole brain total soluble protein are presented. Shortcomings apart (failure to separate membrane proteins), gel-based proteomics remains a viable option, and 2-DGE is the method of choice for generating high-resolution proteome maps of rat brain and brain regions.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2003년도 하계종합학술대회 논문집 V
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• pp.2617-2620
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• 2003

This paper presents the construction algorithm of the irreducible polynomial which needs to multiply over GF(2$\^$m/) and the flow chart representing the proposed algorithm has been proposed. And also, we get the degree from the value of xm＋k formation to the value of k = 7 using the proposed flow chart. The multiplier circuit has been implemented by using the proposed irreducible polynomial generation(IPG) algorithm in this paper, and we compared the proposed circuit with the conventional one. In the case of k = 7, one AND gate and five Ex-or gates are needed as the delay time for the irreducible polynomial in the proposed algorithm, but seven AND gates and sever Ex-or gates in the conventional one. As a result, the proposed algorithm shows the improved performance on the delay time.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송공학회 2001년도 정기총회 및 학술대회
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• pp.9-14
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• 2001

디지털 방송의 등장과 더불어 지상파, 위성, 케이블 등을 통한 다매체 다채널 방송 시청환경에서 시청자가 선택해야 되는 프로그램의 수는 폭발적으로 증가했으며 다양한 시청자의 요구에 부응할 수 있는 시청자 중심의 방송 서비스가 요구되고 있다. 특히 이러한 다채널의 디지털 방송 환경에서는 시청자가 일일이 채널을 바꿔가며 자신이 원하는 프로그램을 찾는 것이 매우 번거롭고 어려워짐에 따라 시청자를 대신하여 시청자의 선호도에 기반한 프로그램을 자동으로 찾아 제시해 줄 수 있는 지능형 프로그램 가이드(intelligent program guide)의 필요성이 대두되었다. 기존의 전자 프로그램 가이드(EPG:Electronic Program Guider)는 단순히 프로그램 관련 정보를 패널별로 정렬된 형태로 사용자 인터페이스를 통해 제시하는 수준에 불과하였다. 본 논문에서는 사용자 선호도 정보에 기반하여 프로그램 정보를 사용자에게 제시하고, 사용자의 프로그램 시청에 대한 액션을 모니터링하여 자동적으로 시청자의 변화하는 선호도를 갱신하는 지능형 프로그램 가이드(IPG:Intelligent Program Guide) 에 대한 연구 결과를 제시한다.

• 한국작물학회지
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• 제60권1호
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• pp.29-32
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• 2015

Two-dimensional electrophoresis (2-DE) was executed to separate the seed storage proteins from the buckwheat. The proteins extracted from the whole seed proteins were better separated and observed in the use of lysis buffer. Using this method, the highly reproducible isoelectric focusing (IEF) can be obtained from polyacrylamide gels, and IEF from the polyacrylamide gel at all the possible pH range (5.0-8.0) was more easily separated than IPG (immobilized pH gradient) gels. The polyacrylamide gels in the first dimension in 2-DE was used to separate and identify a number of whole seed proteins in the proteome analysis. In this new apparatus using 2-DE, 27cm in length of plate coated with polyacrylamide gel was used and the experiment was further investigated under the various conditions.

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2005년도 춘계학술발표대회 개요집
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• pp.80-81
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• 2005

그동안 고출력 레이저에는 CO2 Laser, LPSSL, DPSSL 등이 주로 사용되어 왔다. 최근에는 Fiber Laser가 좋은 빔특성을 가지면서 10kW이상의 고출력이 가능해 큰 주목을 받고 있다. 고출력 레이저는 매질의 냉각문제가 가장 큰 관건인데, Fiber Laser는 수백 ${\mu}m$의 지름을 가진 수십m 길이의 공진기 형태를 띠어 부피 대비 냉각면적이 가장 크다고 할 수 있다. EDFA 둥 광통신을 위해 개발되었던 다이오드 레이저들이 Fiber Laser쪽으로 전용되고, Side Cladding pumping 방법의 실용화, 다이오드레이저 펌핑 광과 광섬유사이의 커플링 방법이 개발되면서 고출력 Fiber Laser 개발이 급속히 이루어졌다. Fiber Laser는 시스템의 부피가 매우 작아질 뿐만 아니라 유지관리 비용이 거의 들어가지 않는다는 장점을 가진다. 현재 단일모드(single-mode) 로는 300W의 출력이 가능하고, 이들을 결합하여10kW 이상의 고출력 Fiber Laser 제품이 나오고 있다. 높은 효율의 레이저발진을 하면서 고출력의 좋은 빔특성을 가지기 때문에 기존의 고출력 레이저용접 및 절단 응용분야에 큰 관심을 불러일으키고 있다.

• KSBB Journal
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• 제18권1호
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• pp.35-38
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• 2003

양수 내에 존재하는 총 단백질을 이차원 전기영동을 이용하여 분리 분석하였고, gradient gel을 이용하여 양수 내에 소량으로 존재하는 미세 단백질까지 분리하였다. 양수 내에는 고농도로 존재하는 단백질이 있는데 이것이 serum albumin precursor임을 확인하였고, 8-18% gradient gel의 이용으로 분해능(resolution)이 향상되어 미세 단백질을 분리 분석할 수 있었다. 이차원 전기영동 후 MALDI-TOF를 이용하여 단백질을 identification하여 기존의 양수 protein database에 존재하는 단백질을 확인하였고, 존재하지 않는 새로운 단백질을 분리 분석하였다.

• International Journal of Advanced Culture Technology
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• 제7권1호
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• pp.77-83
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• 2019

Recently, as social media users have grown, the resulting form of advertising has emerged, and one of the types is native advertising. This advertising method appears to be 52% higher than the banner, according to a study by the IPG Media Lab (2013). Therefore, there is a positive effect on native advertising, but it is necessary to maximize the effect. In this paper, the acceptor's attitude to the native advertisement and the shared intention are verified as to how the effect of the native advertisement can be understood and the maximized effect can be obtained. When the brand phrase was in the form of direct exposure, the subjects were positive about the advertisement and the brand attitude was favorable. Share intentions were also high. These results included suggestions to produce native advertisements in consideration of the attitudes and sharing of the recipients.

• 한국초지조사료학회지
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• 제11권4호
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• pp.230-235
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• 1991

일년생과 다년생식물의 생육환경으로써 토양인산 수준의 영향에 관하여 검토하였다. 1. 일년생의 IRG(Italian ryegrass)와 다년생의 PRG (Perennial ryegrass) 모두 인산시비량이 많을수록 초장이 길어지는 경향을 나타내었다. 2. 2년차 이후는 모든 처리구에서 PRG가 IRG에비하여 건물량이 많았다. 3. 다P처리에 대한 무P처리의 P수량 비율은 1년째부터 3년째에 걸쳐 IPG의 경우 건물량과 같이 매년비율이 커지는 것에 대하여 PRG의 경우는 매년 오히려 처리간의 차가 작아지는 경향을 나타냈다. 4. 다년생의 PRG는 일년생의 IRG에 비하여 고인산환경하에서 보다 저인산 환경하에서의 생육이 유리하여지는 경향을 나타내었다.

• 한국생산제조학회지
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• 제21권4호
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• pp.644-650
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• 2012

This paper show the 2kW fiber laser cutting properties of SS400. The study was comparison of traditional 4kW $CO_2$ Laser cutting and 2kW Fiber laser cutting characteristics for the application of Industrial 2D Laser Cutting Machine. The laser used in this investigation was an IPG YLU-2000 multi-mode Ytterbium Fiber machine with a maximum power of 2000W and a wave length of 1070 nm. The laser was used in its Continuous Wave (CW) mode with an approximately top hat beam intensity distribution. Fiber laser high quality cuts at a large range of speeds (ranging from 2000 to 3800 mm/min) which has been obtained for the 2.3mm Sheet of SS400. 2kW power Fiber laser cut was able to max. 20mm sheets of SS400 (speed range from 650 to 850 mm/min). Fiber laser cutting used in conventional hole nozzle could cut 12mm SS400 but used in special dual cutting nozzle could cut 20mm SS400.