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Proteome Analysis of Amniotic Fluid by gradient 2-D PAGI  

이은희 (아주대학교 화학생물공학부 생명공학전공)
김재찬 (중앙대학교 용산부속병원 안과학교실)
변상요 (아주대학교 화학생물공학부 생명공학전공)
Publication Information
KSBB Journal / v.18, no.1, 2003 , pp. 35-38 More about this Journal
Abstract
Analysis of proteome in amniotic fluid was performed by 2-D PAGE (polyacrylamide gel electrophoresis). Proteins in amniotic fluid were separated by centrifugation and solubilized in buffer solution for IEF, using an IPG strip of pH 4-7L. Both a homogeneous slab gel of 12.5% and a gradient gel of 8-18%, were used. After 2-D PAGE, spots were stained with silver nitrate and picked up for in-gel digestion. Digested peptides were analyzed by MALDI-TOF and proteins were further identifical. More protein spots were detected in the gradient gels and a protein not previously reported was identified.
Keywords
Proteome; amniotic fluid; 2-D PAGE; gradient gel;
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