• Biomolecules & Therapeutics
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• 제31권6호
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• pp.682-691
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• 2023

Cell transformation induced by epidermal growth factor (EGF) and 12-O-tetradecanoylphorbol-13-acetate (TPA) is a critical event in cancer initiation and progression, and understanding the underlying mechanisms is essential for the development of new therapeutic strategies. Licorice extract contains various bioactive compounds, which have been reported to have anticancer and anti-inflammatory effects. This study investigated the cancer preventive efficacy of licochalcone D (LicoD), a chalcone derivative in licorice extract, in EGF and TPA-induced transformed skin keratinocyte cells. LicoD effectively suppressed EGF-induced cell proliferation and anchorage-independent colony growth. EGF and TPA promoted the S phase of cell cycle, while LicoD treatment caused G1 phase arrest and down-regulated cyclin D1 and up-regulated p21 expression associated with the G1 phase. LicoD also induced apoptosis and increased apoptosis-related proteins such as cleaved-caspase-3, cleaved-caspase-7, and Bax (Bcl2-associated X protein). We further investigated the effect of LicoD on the AKT signaling pathway involved in various cellular processes and found decreased p-AKT, p-GSK3β, and p-NFκB expression. Treatment with MK-2206, an AKT pharmacological inhibitor, suppressed EGF-induced cell proliferation and transformed colony growth. In conclusion, this study demonstrated the potential of LicoD as a preventive agent for skin carcinogenesis.

• 한국수자원학회논문집
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• 제33권6호
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• pp.661-669
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• 2000

표사이동에 따른 해빈변형 및 해저지형 변동 그리고 쇄파대 부근에서의 확산거동은 연안역에서의 환경문제를 야기하며 이러한 문제의 해결을 위해서는 해빈류의 유황을 정도높게 예측할 필요가 있다. 이에 따라 기존의 해빈류 수치모형이 몇가지 제안되어 왔지만 실제 해역의 복잡한 해저지형에 의해 발생하는 해빈류를 만족하게 재현하기는 쉽지 않으며 모형의 검증은 현재까지 어려운 과제로 남아있다. 본 연구에서는 실제해역에서의 해빈류에 관한 현지관측 및 수치모형실험을 실시하였으며, 그 결과 현지상황과 대체로 일치하는 흐름상황이 발생하엿음을 알 수 있었다.

• 한국미생물·생명공학회지
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• 제19권4호
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• pp.356-361
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• 1991

식물근부병의 방제균으로 선발된 Bacillus subtilis YBL-7의 식물부균 Fusarium solani에 대한 길항력을 유전공학적 조작에 의해 다목적으로 증강시킬 수 있는지를 타진하기 위해 외부유전자인 Bacillus pasteurii의 urease 유전자를 생물방제균 B.subtilis YBL-7내 도입자하고자 시도하였다. 외부 urease 유전자는 B.pasteurii의 urease gene을 shuttle vector인 pGR71의 HindII site에 삽입하여 E.coli내에서 발현시킨 pGU66을 사용하여 형질전환시켰으며 이때의 최적 형질전환조건과 도입된 urease 유전자의 발현을 조사해 보았다.

• 열처리공학회지
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• 제13권4호
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• pp.253-258
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• 2000

The present study aimed to develop the TRIP(transformation induced plasticity) aided high strength low carbon steel sheets using reverse transformation process. The cold-rolled 0.14C-6.5Mn steel was reverse-transformed by slow heating to intercritical temperature region and air cooling to room temperature. An excellant combination of tensile strength and elongation of $98.3kgf/mm^2$ and 44.4% appears. This combination comes from TRIP phenomena of retained austenite during deformation. The stability of retained austenite Is very Important for the good ductility and it depends on diffusion of carbon and manganese during reverse transformation. The air cooling after holding at intercritical temperature retards the formation of pearlite and provides the carbon enrichment in retained austenite, resulting the increase of elongation in cold-roiled TRIP steel.

• BMB Reports
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• 제51권9호
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• pp.474-479
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• 2018

Cisplatin is one of the most effective chemotherapeutic drugs used in the treatment of HCC, but many patients will ultimately relapse with cisplatin-resistant disease. Used in combination with cisplatin, resveratrol has synergistic effect of increasing chemosensitivity of cisplatin in various cancer cells. However, the mechanisms of resveratrol enhancing cisplatin-induced toxicity have not been well characterized. Our study showed that resveratrol enhances cisplatin toxicity in human hepatoma cells via an apoptosis-dependent mechanism. Further studies reveal that resveratrol decreases the absorption of glutamine and glutathione content by reducing the expression of glutamine transporter ASCT2. Flow cytometric analyses demonstrate that resveratrol and cisplatin combined treatment leads to a significant increase in ROS production compared to resveratrol or cisplatin treated hepatoma cells alone. Phosphorylated H2AX (${\gamma}H2AX$) foci assay demonstrate that both resveratrol and cisplatin treatment result in a significant increase of ${\gamma}H2AX$ foci in hepatoma cells, and the resveratrol and cisplatin combined treatment results in much more ${\gamma}H2AX$ foci formation than either resveratrol or cisplatin treatment alone. Furthermore, our studies show that over-expression of ASCT2 can attenuate cisplatin-induced ROS production, ${\gamma}H2AX$ foci formation and apoptosis in human hepatoma cells. Collectively, our studies suggest resveratrol may sensitize human hepatoma cells to cisplatin chemotherapy via gluta${\gamma}H2AX$mine metabolism inhibition.

• 한국안전학회지
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• 제19권4호
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• pp.1-7
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• 2004

This study investigated the effects of aging on the transformation behavior of $Ti-50.1at\%$ Ni alloy by means of differential scanning calorimetry. It was found that aging in the temperature range of $350^{\circ}C\~550^{\circ}C$ induced complex transformation behavior, involving the R-phase and multiple-stage martensitic transformation. Usually aged Ni-rich NiTi alloys undergo martensitic transformation on cooling from high temperatures in two step : B2 to R and then R to Bl9'(normal behavior). But under certain ageing conditions, the transformation can also occur in three or more step(unusual multiple step behavior). In the present study we use differential seaming calorimetry(DSC) for a systematic investigation of the evolution of transformation behavior with ageing temperature and time.

• 한국해안해양공학회지
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• 제19권6호
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• pp.565-573
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• 2007

타원형 수중천퇴가 있는 지형을 통과하며 변형하는 파랑을 실험한 Vincent and Briggs(1989)의 불규칙파 실험조건을 수치모의하여 파랑과 흐름의 상호작용 효과를 연구하였다. 수치모의를 위해 SHORECIRC(흐름모형)와 REF/DIF S(파랑모형)를 결합한 모형과 파랑과 흐름을 동시에 계산하는 FUNWAVE를 이용하였다. 이 수치모의로부터 수중 천퇴상에서 발생된 쇄파류는 수중천퇴후면의 파 집중현상을 방해하고, 파랑을 천퇴중심축의 바깥쪽으로 굴절시켜, 파고를 상대적으로 감소시키는 역할을 하는 것을 확인할 수 있었다. 결합모형의 수치모의 결과는 쇄파류의 영향을 고려하지 않는 파랑모형만의 결과보다 실험치와 더 일치하였으며, FUNWAVE를 이용한 수치모의도 실험결과와 잘 일치하였다. 이는 파랑쇄파류의 파랑변형에 미치는 역할의 중요성을 확인시켜주는 것이다.

• 전자공학회논문지
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• 제50권4호
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• pp.26-32
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• 2013

전력선에 의한 전자유도 환경에서 가장 보편적 영향 요인이라 할 수 있는 대지의 유도전압 감쇠 효과에 대하여 분석하였다. 전력선은 원통좌표계의 라인소스로 표현되고 계의 분석은 베셀함수와 웨이브 트랜스포메이션에 의한 이의 변형 적용을 통하여 해석된다. 라인소스로부터 방사된 전자계는 지표면에서 반사되어 관측점의 피유도원 매체에 합성되는데 이것의 영향을 알기 위하여 본 논문에서는 베셀함수에 의한 지면 반사계수를 직접 구하여 산출하도록 적용하였다. 본 논문에서 설계된 통상의 유도 환경의 배치 관계에 따르면 지면반사에 의한 원천 유도원 전압과의 합성 효율은 유도전압을 감쇠시키는 차폐효과로서 60~70%정도의 범위를 나타내었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제34권6호
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• pp.602-610
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• 2008

Purpose Acrylamide is present in significant quantities in a wide range of commonly consumed human foods. Carcinogenic risk of acrylamide through the consumption of food is a great public concern and in controversy, but it is not properly addressed due to the lack of evidence in humans. While a plenty of data is available on the carcinogenicity in animal models, the studies in humans are limited. Thus, the present study attempted to examine the carcinogenic potentials of acrylamide on the human epithelial cell, which is the target cell origin of the most cancers. Material and method & Result 1. Acrylamide was not cytotoxic up to $100{\mu}M$ as measured by MTT and LDH assays, indicating a relatively low toxicity of this substance in human epithelial cells. 2. The parameters of neoplastic cellular transformation such as cell saturation density, soft-agar colony formation and cell aggregation were analyzed to examine the carcinogenic potential of acrylamide. 3. The neoplastic transformation was further increased with the co-treatment of TPA 4. Antioxidants blocked the generation of Reactive Oxygen Species(ROS) and the GSH depleting agent dramatically increased the ROS production. 5. mRNA levels of fibronectin following acrylamide exposure was increased in a dose-dependent manner, indicating a possible biomarker of acrylamide-induced cellular transformation. Conclusion The present study will provide a valuable basis to compare the interspecies differences in response to carcinogenic potentials of acrylamide. The data on the interspecies differences are essential element in human risk assessment. Thus, our results obtained from the human epithelial cells will contribute to improving the risk assessment of human neoplasm including oral cancer.

• Applied Microscopy
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• 제45권2호
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• pp.37-43
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• 2015

In present work, work-hardening behavior of TiCu-based bulk metallic glass composite with B2 particles has been studied by systemic structural and mechanical investigations. After yield, pronounced work-hardening of the alloy was clearly exhibited, which was mainly related to the martensitic transformation as well as the deformation twinning in B2 particles during deformation. At the early plastic deformation stage (work-hardening stage), the stress-induced martensitic transformation from B2 phase to B19' phase and deformation-induced twinning of B19' phase was preferentially occurred in the around interface areas between B2 phase and amorphous matrix by stress concentration. The higher hardness value was observed in vicinity of interface within the B2 particles which are probably connected with martensitic transformation and deformation twinning. This reveals that the work-hardening phenomenon of this bulk metallic glass composite is a result of the hardening of B2 particles embedded in amorphous matrix.