Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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ACRYLAMIDE-INDUCED NEOPLASTIC TRANSFORMATION OF HUMAN EPITHELIAL CELLS IN CULTURE

Acrylamide가 인체상피세포의 발암화에 미치는 영향

  • Kim, Sang-Kyu (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyungpook National University) ;
  • Kim, Jin-Wook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyungpook National University) ;
  • Kim, Chin-Soo (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyungpook National University)
  • 김상규 (경북대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 김진욱 (경북대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 김진수 (경북대학교 치의학전문대학원 구강악안면외과학교실)
  • Published : 2008.12.31
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Abstract

Purpose Acrylamide is present in significant quantities in a wide range of commonly consumed human foods. Carcinogenic risk of acrylamide through the consumption of food is a great public concern and in controversy, but it is not properly addressed due to the lack of evidence in humans. While a plenty of data is available on the carcinogenicity in animal models, the studies in humans are limited. Thus, the present study attempted to examine the carcinogenic potentials of acrylamide on the human epithelial cell, which is the target cell origin of the most cancers. Material and method & Result 1. Acrylamide was not cytotoxic up to $100{\mu}M$ as measured by MTT and LDH assays, indicating a relatively low toxicity of this substance in human epithelial cells. 2. The parameters of neoplastic cellular transformation such as cell saturation density, soft-agar colony formation and cell aggregation were analyzed to examine the carcinogenic potential of acrylamide. 3. The neoplastic transformation was further increased with the co-treatment of TPA 4. Antioxidants blocked the generation of Reactive Oxygen Species(ROS) and the GSH depleting agent dramatically increased the ROS production. 5. mRNA levels of fibronectin following acrylamide exposure was increased in a dose-dependent manner, indicating a possible biomarker of acrylamide-induced cellular transformation. Conclusion The present study will provide a valuable basis to compare the interspecies differences in response to carcinogenic potentials of acrylamide. The data on the interspecies differences are essential element in human risk assessment. Thus, our results obtained from the human epithelial cells will contribute to improving the risk assessment of human neoplasm including oral cancer.

Keywords

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