• Journal of Food Hygiene and Safety
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• v.35 no.6
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• pp.630-636
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• 2020

Listeria monocytogenes (L. monocytogenes) was responsible for several recall cases owing to its incidence in mushrooms exported from the Republic of Korea. In this study, we investigated the survival of L. monocytogenes in enoki mushroom (Flammulina velutipes) at different temperatures and the antilisterial effect of its organic acids. Enoki mushrooms were innoculated with L. monocytogenes (initial concentration 4.5 log CFU/g) and stored at 1-35℃, No growth of L. monocytogenes in enoki mushrooms was observed at 1℃ for 30 days. 3.0 log CFU/g growth of L. monocytogenes was also achieved after 36 h and 24 h at 30℃ and 35℃, respectively. To evaluate the antilisterial effect of the organic acids (acetic acid, lactic acid, malic acid), enoki mushrooms were treated with 1-3% of each acid for 10-30 min. The efficacy of malic acid and lactic acid was significantly higher than that of acetic acid. Over 3.0 log reductions were observed when L. monocytogenes in enoki mushrooms was immersed in 3% lactic acid and malic acid over 10 minutes or more. Therefore, it is necessary to keep enoki mushrooms at 1℃ during the export process and treat them with 3% lactic acid and malic acid for 10 min prior to consumption.

• Journal of Food Hygiene and Safety
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• v.35 no.6
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• pp.544-551
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• 2020

The aim of this study was to develop and validate a simultaneous analytical method for berberine and palmatine, which are representative substances of Phellodendron amurense, and to evaluate the antioxidant activity. We evaluated the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of analytical methods for berberine and palmatine using high-performance liquid chromatography. Our result showed that the correlation coefficients of the calibration curve for berberine and palmatine exhibited 0.9999. The LODs for berberine and palmatine were 0.32 to 0.35 µg/mL and the LOQs were 0.97 to 1.06 µg/mL, respectively. The inter-day and intra-day precision values for berberine and palmatine were from 0.12 to 1.93 and 0.19 to 2.89%, respectively. The inter-day and intra-day accuracies were 98.43-101.45% and 92.39-100.60%, respectively. In addition, the simultaneous analytical method was validated for the detection of berberine and palmatine. Moreover, we conducted FRAP and NaNO2 scavenging activity assays to measure the antioxidant activities of berberine and palmatine, and both showed antioxidant activity. These results suggest that P.amurense could be a potential natural resource for antioxidant activity and that the efficacy can be confirmed by investigating the content of the berberine and palmatine.

• Tunnel and Underground Space
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• v.31 no.4
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• pp.289-308
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• 2021

This study conducts coupled thermo-hydro-mechanical numerical modeling to investigate the maximum temperature and conditions for securing mechanical stability of the high-level radioactive waste repository when temperature criteria of bentonite buffer are 100℃ and 125℃, respectively. In case of temperature criterion of buffer as 100℃, the maximum temperatures at the interface between canister and buffer are calculated to be 99.4℃ and 99.8℃, respectively for a case with disposal tunnel spacing of 40 m and deposition hole spacing of 5.5 m and for the other case with disposal tunnel spacing of 30 m and deposition hole spacing of 6.5 m. In case of temperature criterion of buffer as 125℃, spacings of disposal tunnel and deposition hole could be decreased to 30 m and 4.5 m, respectively, which reduces the disposal area up to 55% compared to the disposal area of KRS⁺. According to analysis of mechanical stability for various disposal spacings, RMR of rock mass for KRS⁺ should be larger than 72.4 which belongs to good rock in RMR classification to prevent failure of rock mass. As disposal spacing is decreased, required RMR of rock mass is increased. In order to prevent failure of rock mass for a case with disposal tunnel spacing of 30 m and deposition hole spacing of 4.5 m, RMR larger than 87.3 is needed. However, mechanical stability of the repository is secured for all cases with RMR over 75 considering the enhancement of rock strength due to confining stress induced by swelling of the bentonite buffer and backfill.

• Korean Journal of Remote Sensing
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• v.38 no.6_2
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• pp.1607-1622
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• 2022

The purpose of this study is to determine the actual performance of cool roof in preventing absorbed solar radiation. The spatial correlation between surface temperature and absorbed solar radiation is the method by which the performance of a cool roof can be understood and evaluated. The research area of this study is the vicinity of Jangyu Mugye-dong, Gimhae-si, Gyeongsangnam-do, where an actual cool roof is applied. FLIR Vue Pro R thermal infrared sensor, Micasense Red-Edge multi-spectral sensor and DJI H20T visible spectral sensor was used for aerial photography, with attached to the drone DJI Matrice 300 RTK. To perform the spatial correlation analysis, thermal infrared orthomosaics, absorbed solar radiation distribution maps were constructed, and land cover features of roof were extracted based on the drone aerial photographs. The temporal scope of this research ranged over 9 points of time at intervals of about 1 hour and 30 minutes from 7:15 to 19:15 on July 27, 2021. The correlation coefficient values of 0.550 for the normal roof and 0.387 for the cool roof were obtained on a daily average basis. However, at 11:30 and 13:00, when the Solar altitude was high on the date of analysis, the difference in correlation coefficient values between the normal roof and the cool roof was 0.022, 0.024, showing similar correlations. In other time series, the values of the correlation coefficient of the normal roof are about 0.1 higher than that of the cool roof. This study assessed and evaluated the potential of an actual cool roof to prevent solar radiation heating a rooftop through correlation comparison with a normal roof, which serves as a control group, by using high-resolution drone images. The results of this research can be used as reference data when local governments or communities seek to adopt strategies to eliminate the phenomenon of urban heat islands.

• Journal of the Korean Orthopaedic Association
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• v.56 no.5
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• pp.398-403
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• 2021

Purpose: A biopsy is needed to diagnose soft tissue tumors. However, it is extremely difficult to pinpoint the site of a tumor due to the heterogeneity of sarcomas. Thus, even when an open biopsy is conducted, it is difficult to diagnose a soft tissue tumor. In such cases, an ultrasound (US)-guided biopsy is used to improve the diagnostic accuracy. This study evaluated the accuracy of US-guided biopsy for a diagnosis of soft tissue tumors found initially in a magnetic resonance (MR) perfusion and assessed the availability of positron emission tomography-computed tomography (PET-CT) for a diagnosis of soft tissue tumors. Materials and Methods: From January 2014 to December 2018, the US-guided biopsy was performed on 152 patients with a suspected soft tissue tumor found in an MR perfusion and 86 cases were definitively diagnosed with a soft tissue tumor. The accuracy of the US-guided biopsy was assessed retrospectively. Among the 86 cases, only MR perfusion was used before the biopsy in 50 cases, while both MR perfusion and PET-CT was conducted on 36 cases. The accuracy was analyzed to determine if the PET-CT could improve the precision of a biopsy. Results: From 86 cases, 34 out of 50 cases, in which only MR perfusion had been conducted, matched the result of the definitive diagnosis and the US-guided biopsy. 32 out of 36 cases, in which both PET-CT and MR perfusion were conducted, matched the definitive diagnosis and the US-guided biopsy. These results show significant differences in the accuracy of US-guided biopsy. In the case of soft tissue sarcomas, 6 out of 12 cases, in which only MR perfusion had been conducted, matched the result of the definitive diagnosis and the US-guided biopsy. 17 out of 18 cases, in which both PET-CT and MR perfusion were conducted, matched the definitive diagnosis. Moreover US-guided biopsy also showed significant differences in the accuracy of US-guided biopsy. Conclusion: In diagnosing soft tissue tumors, a US-guided biopsy is a well-known tool for its high accuracy. However, the heterogeneity of sarcoma makes it difficult to locate the exact site for a biopsy using only MR perfusion. Thus, the use of PET-CT will meaningfully improve the accuracy of a diagnosis by precisely targeting the site for the US-guided biopsy.

• Journal of Food Hygiene and Safety
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• v.36 no.6
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• pp.528-536
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• 2021

The purpose of the study was to investigate the content of sinigrin, an index component, in Brassica juncea extract and to evaluate the differentiation of lipocytes, inhibition of production of reactive oxygen species (ROS) and reduction of protein production by lipogenic factors (PPARγ, C/EBPα, aP2) in the processing of Brassica juncea extract and sinigrin in 3T3-L1 preadipocytes which induces Bisphenol A (BPA), an endocrine disrupting environmental hormone. From the investigation, the content of sinigrin in Brassica juncea extract, measured by HPLC, is found to be 21.27±0.2 mg/g. The XTT assay result on BPA-derived 3T3-L1 adipocytes shows there is no cytotoxicity found from 180 µM of sinigrin and 300 ㎍/mL of Brassica juncea extract. Moreover, both intracellular lipid accumulation and ROS production during differentiation of lipocyte are significantly reduced in cells processed with Brassica juncea extract and sinigrin. Lastly, it was also found that the production of transcription factors of lipocyte differentiation, PPARγ, C/EBPα and aP2, were found to be suppressed by the application of Brassica juncea extract and sinigrin. Such results reveals that Brassica juncea is effective in not only suppressing lipid accumulation in the environmental hormone bisphenol A-derived lipocyte, but also in reducing the ROS. The sinigrin-containing Brassica juncea is highly expected to be used in natural functional supplements that prevents the lipid metabolism disorders caused by BPA. There are necessities for additional clinical research and follow-up studies on the in vivo model to verify the relevant mechanisms.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.1
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• pp.1-10
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• 2022

Customized cosmetics are continuously mentioned as a trend in the cosmetics industry to respond to the recent rapid changes in the social environment and pursue individuality and diversity. Accordingly, four types of liposome essence corresponding to skin types were prepared by varying the ratio of liposome formulation and essence formulation as a customized cosmetic base that can be easily mixed and applied at the workplace. The volatilization residues of four types of liposome essence were measured and the nanoparticle size, polydispersity index, zeta potential and viscosity according to time for 90 d were measured, and Turbiscan was measured as a method for evaluating the stability of a colloidal dispersion system. In addition, a simple usability evaluation was performed for four types of liposome essence corresponding to the skin type. As a result, the amount of volatile residue in the four types of liposome essence was increased in dry products rather than oily ones, and the particle size showed a tendency to increase with time in the range of 165 to 175 nm, increasing up to 31.5%, and the polydispersity index was 0.23 to 0.26. There was little change with time, and the zeta potential was -74 to -72 mV, showing a slight decrease with time, but there was little change to the extent of a maximum decrease of 14.0%. Viscosity showed a decreasing trend with time in the range of 2,580 ~ 3,290 cps, showing a maximum decrease of 17.5%. In the turbiscan measurement, all of the turbiscan stability index, a measure of stability, were less than 1.0, indicating dispersion stability. In the overall simple usability satisfaction evaluation for skin types (6 points), products for oily skin (5.33 ± 0.75 points) > products for medium dry skin (5.13 ± 0.95 points) > products for dry skin (5.03 ± 0.96 points) > products for oily skin (4.80 ± 1.04 points) points) were evaluated in order. The four types of liposome essence corresponding to skin types with different ratios of liposome formulation and essence formulation were physically stable, and the possibility of application as a customized cosmetic base according to skin type was confirmed.

• Journal of Life Science
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• v.32 no.10
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• pp.784-791
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• 2022

This study observed changes in the body composition of Protaetia brevitarsis sinulensis larvae when reared with feed that included noni and nipa palm. Compared to the control group, the death rate and product yield of the treatment group were improved after the larval fasting process. The brightness of the larval powder of the treatment group increased, but the redness decreased. The crude fat content of the treated group was slightly increased according to the assays of the general components, but the mineral content was significantly increased. The total structural amino acids of the treatment group decreased, but the total free amino acids increased. The structural amino acids generally tended to decrease in the treatment group. However, the free amino acids showed no specific differences. Among the free amino acids, tryptophan, phosphoserine, and methylhistidine were highly increased in the treatment group, whereas threonine, methionine, and asparagine showed the opposite results. Among the polyunsaturated fatty acids, eicosapentaenoic acid (C20:5n3) of omega-3 was increased in the treatment group, but the omega-6 series was decreased. Since minerals, total free amino acids, and omega-3 fatty acids in the treatment group were increased compared to the control group, we suggest that noni and nipa palm can potentially be applied to the production of functionally improved substances as additional sources of feed for Protaetia brevitarsis sinulensis larvae.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.2
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• pp.165-170
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• 1994

High quality rapeseed cultivars including Nojeokchae, Yeongsanyuchae Halla-yuchae and Tamrayuchae have been released and recommended as a zero erucic acid variety to Cheju farmers for 13 years, where is a major rapeseed production area in korea. However, rapeseeds produced in Cheju island in 1992 and 1993 contained 47.7% and 37.0% of erucic acid respectively resulting in poor quality oil being not adequate for edible oil. It was considered that the zero erucic acid varieties did not have an opportunity to be cultivated in Cheju island by farmers living in the Island. Thus, the new rapeseed varieties without erucic acid should be bred and recommended to the farmers of southern area of Korea as a multiple cropping crop just after rice harvest, and for large scale mechanized and labour-serving rapeseed culture. The change of rapeseed breeding goal would be desirable for fatty acid composition improvement of rapeseed to develop varieties adaptable to southern part of Korea, and to produce rapeseed oil directly used as an edible oil safely.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.5
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• pp.580-586
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• 1995

Soil temperatures at depths of 1~5cm are important to the germination and emergence of dry seeded-rice. An automated weather station was used to monitor the hourly weather parameters at Experiment Farm, Kyung Hee University from April 21 to May 30 in 1994. The data was analyzed to figure out the 24-hour temporal changes in air 1.5m above ground and soil temperatures under ground of 0, 2.5, 5, 10 and 20cm. The fluctuations of soil temperature were greatest at the soil surface and decreased with increasing depth. Mean soil temperatures at depth of 2.5cm were about 3$^{\circ}C$ higher than mean air temperatures during the observation period. Although mean soil temperatures at depth of 2.5cm during 10 or 15 days after April 21, May 1 and May 11 showed almost same temperatures, the distribution patterns of temperature regime were different from each other. Rice cultivars, Hwasung, Seohae, Nampung, IR60 and CR155, were seeded at depth of 2.5cm on April 21, May 1 and May 11, respectively. The periods of seedling emergence(PSE) varied in accordance with cultivars and seeding dates. PSE was correlated with accumulated daily mean air temperatures and accumulated hours classified by temperature regimes.