• Animal cells and systems
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• 제12권3호
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• pp.137-141
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• 2008

Molecular cloning revealed the three isoforms($Ca_v3.1,\;Ca_v3.2,\;and\;Ca_v3.3$) of the T-type calcium channel subfamily. Expression studies exhibited their distinctive electrophysiological and pharmacological properties, accounting for diverse properties of T-type calcium channel currents previously characterized from isolated cells. However, electrophysiological properties of ion channels have shown to be more diversified by their splice variants. We here searched splice variants of rat $Ca_v3.1$ T-type channel by reverse-transcription-polymerase chain reaction(RT-PCR) to further explore diversity of $Ca_v3.1$. Interestingly, analyses of cloned RT-PCR products displayed that there were at least four splicing variants of rat $Ca_v3.1$ in the loop connecting repeats III and IV. Southern blot analyses indicated that the predominantly detected variant in brain was $Ca_v3.1a$(492 bp), which were rarely detected in most of peripheral tissues. Other two variants($Ca_v3.1c$, 546 bp; $Ca_v3.1d$, 525 bp) were detected in most of the tissues examined. The smallest isoform($Ca_v3.1b$, 471 bp) was rarely detected all the tissues. Electrophysiological characterization of the splicing variants indicated that the splice variants differ in inactivation kinetics and the voltage dependence of activation and inactivation as well.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1995년도 추계학술발표회논문집(1)
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• pp.403-410
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• 1995

A loss of the residual heat removal (RHR) accident during mid-loop operation of Yong-gwang Nuclear Units 3/4 was analyzed using the RELAP5/MOD3.1.2 code. In this work the following assumptions are used; (i) initially the reactor coolant system (RCS) above the hot leg center line is filled with nitrogen gas, (ii) two 3/4-inch diameter vent valves on the reactor vessel head and the top of pressurizer in the reactor coolant system are always open, and a level indicator is connected to the RMR suction line, (iii) the two steam generators are in wet layup status and the steam generator atmospheric dump valve assemblies are removed so that the secondary side pressure remains at nearly atmospheric condition throughout the accident, and (iv) the loss of RHR is presumed to occur at 48 hours after reactor shutdown. Findings from the RELAP5 calculations are (i) the core boiling begins at ∼5 min, (ii) the peak RCS pressure is ∼3.0 bar, which implies a possibility of temporary seal break, (iii) ∼94 % of the decay heat is removed by reflux condensation in the steam generator U-tubes in spite of the presence of noncondensable gas, (iv) the core uncovery time is evaluated to be 7.2 hours. Significant mass errors were observed in the calculations.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1996년도 춘계학술대회
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• pp.203-206
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• 1996

To understand the local fluid dynamics for different desists of Fontan operation, five models were made out of Pyrex glass to facilitate in-vitro study. Model I, II and III have same position of the center of the anastomosis of the IVC( inferior vena cava) with that of the SVC(superior vena cava), but Model IV and V have 10 mm offset between them. Also the anastomotic junction angles are different(Model I and $IV:90^{\circ}$, Model II and $V:70^{\circ}$, Model $III:45^{\circ}$). These models were then connected to a flow loop for flow visualization study. In Model I any dominant vortex was not seen in the central region of the juntion, but a large unstable vortex was created in the Model II and III. In Model IV and V a significant stagnation region was created in the middle of the offset region. It also showed that the flow direction from the IVC and SVC to the LPA(left pulmonary artery) and RPA(right pulmonary artery) highly depends on the offset of the junction rather than the anastomotic junction angle.

• 대한전자공학회논문지SD
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• 제42권11호
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• pp.23-32
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• 2005

회로도면 자동생성 분야는 지난 수십 년간 HDL기반 설계과정에서 사용되어 왔다. 그러나 회로 도면은 더욱 복잡해져서 레지스터 및 시스템 레벨에서 자동 생성된 회로도면을 보고 신호의 흐름을 파악하기 어렵다. 이와 같이 복잡해진 회로도면의 가독성을 향상시키기 위해 본 논문에서는 4가지 기법, 즉 i ) 심볼이나 터미널들과 같이 반복되는 회로 패턴을 벡터 형태로 치환, ii) 피드백 루프 절단 알고리즘 개선, iii) 번들 네트 생성시 발생하는 다단 연결을 간결 화할 수 있는 압축 탭, iv) 연결도에 따라 블록열을 구분하고 정렬하는 알고리즘을 제안한다. 제안된 회로도면 생성 기법의 효용성을 확인하기 위해 도면 자동생성 프로그램을 개발하고, 계층적으로 설계된 미디어 프로세서의 다양한 모듈의 도면을 생성시켰다. 실험한 결과 도면 면적을 비롯하여 배선 수, 길이 등을 $90\%$까지 감소시키고 가독성을 높이는 효과를 보였으며 블록의 분산 및 빈 공간 발생을 억제하는 효과를 보였다.

• 농업과학연구
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• 제47권1호
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• pp.173-182
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• 2020

Human Astrovirus (HuAstV), known as a waterborne virus, is a group IV positive-sense single-stranded RNA that belongs to Astroviridae. The first outbreak of HuAstV was reported in England in 1975. HuAstV can exist not only among clinical patients but also in various water environments, such as water for agriculture and vegetables. For diagnosis of HuAstV from water samples, a polymerase chain reaction (PCR) system has been developed. However, the PCR-based diagnostic method has problems in field application, such as reaction time, sensitivity and specificity. For this reason, in this study we developed the loop-mediated isothermal amplification assay (LAMP) system, aimed specifically at HuAstV. Three prepared LAMP primer sets were tested by specificity, non-specificity and sensitivity; one LAMP primer set was selected with optimum reaction temperature. The developed LAMP primer set reaction conditions were confirmed at 62℃, and detection sensitivity was 1 fg/μL. In addition, restriction enzyme HaeIII (GG/CC) was introduced to confirm that the LAMP reaction was positive. As a result, selected LAMP primer set was 100 - 1000 times more specific, rapid, and sensitive than conventional-nested PCR methods. For verification of the developed LAMP assay, twenty samples of cDNA from groundwater samples were tested. We expect that the developed LAMP assay will be used to diagnose HuAstV from various samples.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2004년도 학술대회 논문집 정보 및 제어부문
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• pp.708-710
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• 2004

This paper studies a multirate sampled-data control for LTI systems by using the digital redesign (DR) method. In this note, to well tackle the problem associated with both the state matching and the stabilization, our nobel strategy is to minimize the linear quadratic cost function. The main features of the proposed method are that i) the delta-operator-based descretization method is applied to improve the state-matching performance in the fast sampling limit and/or the large input multiplicity; ii) the proposed multirate control scheme can improve the state-matching performance in the long sampling limit; iii) some sufficient conditions that guarantee the stability of the closed-loop discrete-time system and provide a guarantee cost for the cost function can be formulated in the LMIs format; and iv) an optimal sampled-data controller in the sense of minimizing the upper bound of the cost function is also given by means of an LMI optimization procedure.

• BMB Reports
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• 제37권3호
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• pp.330-338
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• 2004

Different types of cardiotoxin (I-V and n) were isolated and purified from the venom of the Taiwan cobra (Naja naja atra). The effects of these cardiotoxins were studied on membrane-bound acetylcholinesterase, which was isolated from a sheep's brain cortex. The results showed that cardiotoxins I-III, V, and n activated the enzyme by modification of substrate inhibition, but cardiotoxin IV's reaction was different. The inhibition and activation of acetylcholinesterase were linked to the functions of the hydrophobicity index, presence of a cationic cluster, and the accessible arginine residue. Our results indicate that Cardiotoxins have neither a cationic cluster nor an arginine residue in their surface area of loop I; therefore, in contrast to fasciculin, cardiotoxins are attached by loop II to the peripheral site of the enzyme. As a result, fasciculin seems to stabilize nonfunctional conformation, but cardiotoxins seem to stabilize the functional conformation of the enzyme. Based on our experimental and theoretical findings, similar secondary and tertiary structures of cardiotoxins and fasciculin seem to have an opposite function once they interact with acetylcholinesterase.

• 대한기계학회논문집A
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• 제37권10호
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• pp.1251-1259
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• 2013

본 연구에서는 소듐냉각 고속로 붕괴열교환기(DHX)의 고온 설계 및 크리프-피로 손상 평가를 수행하였다. 제 4 세대 소듐냉각 고속로의 능동 및 피동 잔열제거계통에 설치되는 DHX와 한국원자력연구원의 STELLA-1 시험루프에 설치된 DHX에 대해 상세설계 및 3D 유한요소해석을 수행하고, 동 결과에 기초하여 고온설계 기술기준인 ASME Section III Subsection NH와 RCC-MR 코드를 따라 크리프-피로 손상평가를 수행하였다. 크리프-피로 손상평가 결과에 기초하여 두 설계기준에 대해 비교 분석하고, 설계 기술기준의 보수성 이슈에 대해 토의하였다.

• Radiation Oncology Journal
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• 제15권2호
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• pp.113-119
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• 1997

목적 : 국소적으로 진행된 위암치료는 수술이 주 치료로 5년 생존율은 그리높지 않아 좀더 완치율을 높이기 위하여 화학요법이나 화학요법과 병용하여 방사선치료 등이 시행되고 있다 저자들은 위암환자에서 수술후 방사선치료를 받은 환자의 치료성적을 후향적으로 분석하였다. 방법 : 1985년 3월부터 1993년 6월까지 한양대학병원 치료방사선과에서 위절제수술후 방사선 치료를 3500cGy 이상 받고 병기 3및 4기인 68명을 대상으로 후향적인 분석을 하였다. 연령분포는 28세부터 66세였고 중앙연령은 50세였다. 추적기간은 3개월에서 133개월(3명이 36개월 미만)로 중앙값은 50개월이었다. 조직병리상 37명이 non signet ring adenocarclnoma였고 29명이 signet ring cell이었다. 병기로 IIIA가 19명, IIIB가 25명, IV가 24명이었다. 항암화학요법은 65명의 환자에게 투여되었으며 FAM계통의 치료가 28예 cispiatln, 5FU계통이 26예이었다. 결과 : 5년생존율은 36.6% 5년무병생존율은 33.6%였다. 예후인자로 병소침입 임파선개수, 조직세포 종류 등이 생존율에 통계적으로 유의하게 영향을 주었고 잔존세포의 유무, 병기, 나이, 세포분화도 및, 방사선치료 총 기간 등도 생존율에 영향을 주었으나 통계적인 유의성은 없었고 항암화학제의 종류에따른 생존율의 차이는 없었다. 37명환자에서 재발이 있었고 위치로는 장망(omentum) 및 복막에 23.5%로 가장 재발율이 놀았으며 남은 위나 봉합부위에 13.2%의 재발율을 보였다. 전체 국소재발은 20.7% 전체 원격전이는 39.7%였다. 방사선치료로 인한 부작용은 22.1%에서 grade 3의 백혈구감소가 있었고 1.5%에서 grade 4 부작용이 있었다. 치료중 10% 이상의 체중감소는 8.8%였다. 결론 : 본 연구결과 수술후 화학요법과 방사선치료요법은 큰 부작용이 없었으며 실패양상은 주로 장망 및 복막이었고 치료부위내 국소재발율이 다음으로 많았다. 국소재발은 원격전이에 비해 많이 낮아지는 추세를 보였다 방사선역할에 평가를 조금 더 명확하게 규명하기위해 향후 재발 위험이 큰 환자에서 수술후 화학요법만 한 군과 화학요법 및 방사선치료를 병용한 군과의 재발 양상 및 생존율 등의 전향적 비교 연구가 필요할 것으로 생각되며 더 나아가 수술후 항암화학요법 및 방사선 병용요법에 복강내 약물치료도 고려되어야 하겠다.

• Journal of Ginseng Research
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• 제33권1호
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• pp.59-64
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• 2009

$Ca^{2+}$ and calmodulin (CaM), a key $Ca^{2+}$ sensor in all eukaryotes, have been implicated for defense responses of plants. Eukaryotic CaM contains four structurally and functionally similar $Ca^{2+}$ domains named I, II, III and IV. Each $Ca^{2+}$ binding loop consists of 12 amino acid residues with ligands arranged spatially to satisfy the octahedral symmetry of $Ca^{2+}$ binding. To investigate the altered gene expression and the role of CaM in ginseng plant defense system, cDNA clone containing a CaM gene, designated PgCaM was isolated and sequenced from Panax ginseng. PgCaM, which has open reading frame of 450 nucleotides predicted to encode a precursor protein of 150 amino acid residues. Its sequence shows high homologies with a number of other CaMs, with more similarity to CaM of Daucus carota (AAQ63461). The expression of PgCaM in different P. ginseng organs was analyzed using real time PCR. The results showed that PgCaM expressed at different levels in young leaves, shoots, and roots of 3-week-old P. ginseng. In addition, the expressions of PgCaM under different abiotic stresses were analyzed at different time intervals.