• 경영과학
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• 제33권1호
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• pp.17-33
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• 2016

This study investigated the relationships among proper types of e-commerce, patterns of inter-organizational information flow (IIF), benefits of e-commerce, and supply-chain performance. In IIF, it was empirically observed that environmental competition positively influences the frequency of IIF, and environmental uncertainty has a positive impact on the quality of IIF. It was also empirically confirmed that only quality of IIF has effects on the usage levels of e-procurement as well as e-partnerships. Through regression analyses and cluster analysis, it was found that under high degrees of the frequency and quality of IIF, the benefits of e-commerce are vastly realized in the firms that highly utilize e-partnerships. The results of this study also showed that when the degrees of quality of IIF are high, the levels of benefits of e-commerce are increased in the firms that employ e-procurement. The moderating effects of buyer and supplier firms' abilities on the relationships between patterns of IIF and usage levels in various types of e-commerce were empirically examined. According to the results, the moderating roles of buyer firms' capabilities and suppliers' abilities were partially demonstrated. Finally, it was found that the realization of benefits of e-commerce can lead to the improvement of supply-chain performance.

• Restorative Dentistry and Endodontics
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• 제21권1호
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• pp.1-18
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• 1996

There are many advantages when using IIF and DNA probe methods over anaerobic culture method in that they are time-and effort-saving, more precise and more sensitive. Furthermore, in IIF and DNA probe methods, the detection is possible only with small amount of bacteria, the quantitative analysis is possible, and the cell viability is not necessary. The purpose of this study is to observe the incidence of P.endodontalis by carrying out anaerobic culture, IIF and colony lift using DNA probe method respectively, and to compare these 3 methods in terms of effectiveness and sensitivity in order to identify the most effective detection method. 30 teeth with at least one clinical symptoms, with single canal, and with pulp necrosis were sampled. For sampling bacteria, access cavity was prepared after disinfecting tooth and its surroundings. Then the paper point was inserted up to the periapical area, leave there for a while, and finally it was placed into PRAS Ringer's sol. and PBS sol. In anaerobic culture method, P.endodontalis was identified by biochemical tests after subculturing black and brown colonies which were produced after 7 days of incubation on BAP and Brucella BAP in anaerobic chamber. To identify P.endodontalis in IIF method, species-specific polyclonal rabbit-antisera of P.endodontalis(ATCC 35406) was reacted with sampled PBS sol. dispensed onto glass slide, and then P.endodontalis was examined by phase contrast microscopy after incubating with Goat anti-rabbit lgG conjugated to Fluorescein isothiocyanate. For colony lift using DNA probe method, membranes were laid over colonies on the surface of BAP and were hybridized with cloned DNA probe of P.endodontalis. The existence of P.endodontalis was then identified by the methods of chemiluminescent detection and color metric detection. Black colony was found in 11 teeth out of 30 teeth and P.endodontalis was detected in 6 teeth (20 %) by anaerobic culture method, 16 teeth (53 %) by IIF method, and 7 teeth (23 %) by DNA probe method. IIF method is significantly better in detecting P.endodontalis than DNA probe method and anaerobic culture method. There was no significant differences between DNA probe method and anaerobic culture method. There was significant correlation between the formation of black colony and the existence of P.endodontalis. The probability of detecting P.endodontalis when black colony being present is 2.89 times higher than when not being present. There was significant relationship between the foul odor of clinical symptoms and P.endodontalis. The sensitivity of existing P.endodontalis when foul odor being present was 93.75 %, while the specificity of not existing P.endodontalis when foul odor not being present was 28.57 %. These results suggested that the probes of P.endodontalis will be used to decide the method and prognosis in endodontic treatments.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2002년도 봄 학술발표논문집 Vol.29 No.1 (B)
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• pp.103-105
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• 2002

이 논문에서는, PC 클러스터 환경에서 질의 확장을 사용하는 정보 검색 시스템 (IR)을 설계하고 구현한 내용을 기술한다. 이 정도 검색 시스템은 문서 집합을 저장하고, 문서 집합은 역색인 파인 (IIF)로 색인되고, 랭킹 방법으로 벡터 모델을 사실하며, 질의 확장 방법으로 코사인 유사도를 사용한다. 질의 확장이란 사용자가 준 원래의 질의에 연관된 단어를 추가하여 검색 효율을 향상시키는 것이다. 여기서 제안하는 병렬 정보 검색 시스템에서는 역색인 과일은 여러 개로 분활되는데 lexical 분할 방법과 greedy 분할 방법을 사용한다. 사용자의 질의가 들어오면 질의확장을 하여 여러 개의 단어로 이루어진 확장된 질의가 만들어 지는데 이 확장된 질의를 구성하는 단어들은 각 단어와 연관된 IIF를 가지고 있는 노드에 보내어져서 병렬로 처리된다. 실험을 통하여 병렬 IR 시스템의 성능이 질의 확장과 IIF의 두 가지 분한 방법에 의해 어떻게 영향을 받는지 보인다. 실험에는 표준 한국어 테스트 말뭉치인 EKSET과 KTSET을 사용하였다. 실험에 따르면 greedy 분활 방법이 lexical 분할 방법에 비해 20%정도의 성능 향상을 보였다.

• 대한수의학회지
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• 제44권2호
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• pp.259-268
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• 2004

Porcine circovirus type 2 (PCV-2) has been associated with various disease in pigs worldwide including postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). In this study, monoclonal antibodies (MAbs) against PCV were produced, characterized and applications of MAbs as diagnostic reagents were described. Spleen or lymph node cells from BALB/c mouse immunized respectively with PCV-1, PCV-2 or expressed PCV-2/ORF2 proteins in baculovirus were fused with SP2/0 myeloma cells using polyethylene glycol (PEG) and hybridoma cells producing PCV-1 or PCV-2-specific antibody were screened by an indirect immunofluorescence (IIF) test. A total of fifteen MAbs were produced against PCV. Six MAbs were PCV-1-specific and nine were PCV-2-specific. All PCV-1-specific MAbs reacted with only PCV-1 and all PCV-2-specific MAbs were reactive with only PCV-2 by IIF test. None of the MAbs was reactive with porcine reproductive and respiratory syndrome virus (PRRSV), porcine parvovirus (PPV), porcine rotavirus (PRV), and transmissible gastroenteritis virus (TGEV). Some PCV-2-specific MAbs recognized the PCV-2 infected porcine tissues by IIF or immunohistochemistry (IHC) assay. From this experiment, it was confirmed that MAbs produced in this study were PCV-specific and could be used as reliable diagnostic reagents for PCV-1/PCV-2 detection and differentiation.

• 대한수의학회지
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• 제27권2호
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• pp.191-200
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• 1987

Attempts were to produce porcine leukocyte interferon(PorLeIF) and porcine immune interferon (PorIIF) in the culture of porcine leukocytes. The interferons produced were tested for antiviral activity against vesicular stomatitis virus on poreine-derived PK(15) cells, human-derived FL cells, and Korean native black goat-derived BGK cells. The results were summarized as follws: 1. In the isolation of porcine leukocytes, the mean isolation rate by the buffy coat separation method (28.7%) was higher than that by the hydroxyethyl starch-RBC sedimentation method (9.2%). 2. When NDV(BI)-induced PorLeIFs were assyed on PK(15) cells and FL cells, the mean titers were 129 IU/ml and 72 IU/ml respectively, being 55.8% of the activity in homologous species system expressed in heterologous system. 3. The activities of PHA P-induced PorIIFs were 197 IU/ml on PK (15) cells and no activity on human FL cells. The mean antiviral activity of PorIIF was 1.5 times that of PorLeIF in PK (15) cells. 4. The cytopathic effect of vesicular stomatitis virus was observed in BGK cells derived from Korean native black goat kidney permitting interferon assay on the cells. While the cross-species antiviral activity of reference human ${\alpha},\;{\beta}-interferon$ was observed on the cells, PorLeIF and PorIIF did not show any activity.

• 한국어병학회지
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• 제35권2호
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• pp.241-246
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• 2022

White spot syndrome virus (WSSV) is a prevalent and virulent pathogen affecting cultured whiteleg shrimp (Litopenaeus vannamei) in Korea. In this study, seven monoclonal antibodies (mAbs) (10A12, 16C3, 17G4, 21G5, 22C4, 23B6 and 24G6) were produced by using purified WSSV. The reactivity of these mAbs was analysed by Western blot (WB), indirect immunofluorescence (IIF), and lateral flow immunochromatographic assay (LFIA). WB analysis demonstrated that three mAbs (17G4, 22C4, and 23B6) reacted specifically to VP28 with an approximate molecular weight of 24 kDa, mAb 16C3 reacted with approximately 17 kDa. IIF analysis demonstrated specific fluorescence signals on gill tissues of WSSV-infected shrimp, with five mAbs (10A12, 16C3, 22C4, 23B6, and 24G6), pleopods from WSSV-infected shrimp were used for LFIA, where, two mAbs (21G5 and 22C4) exhibited positive reaction. In conclusion, it can be inferred that the mAbs usage and specificity depends on the nature of assay used for diagnosis.

• Parasites, Hosts and Diseases
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• 제33권2호
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• pp.107-116
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• 1995

국내 마우스에서 분리된 작은와포자충(Cwptospoyidinpown)을 마우스에서 증식시킨 다음 오오시스트만을 순수 분리하여 BAIB/c 마우스에 면역시켰다. 면역된 마우스의 비장에서 림프구를 분리하여 PEG 1500을 융합촉진제로 사용하여 Sp2/0 myeloma cell과 세포융합을 실시하였으며, 융합된 세포중 작은와포자충에 특이항체를 생산하는 hybridomacell을 선발하였다 한계희석법으로 제작된 2주의 단세포군항체는 IgG2b class(lE7.2)와 IgM class(C6)에 속했으며. SDS-PAGE와 Westernblotting한 결과 (IE7.2)는 원충의 단백항원중 36 kDa과 반응하였고, C6는 67 kDa 및 70 kDa과 반응하였다 생산된 단세포군항체를 간접 형광항체법으로 작은와포자충과 반응시킨 결과 오오시스트 외막체 특이적으로 반응하였던 반면, Tomplasma gondii의 tachyzoite, Eimeria zuernii E. bouis, E. conodensis의 오오시스트와는 반응을 나타내지 않았다. 단세포군항체 C6을 이용한 간접 형광항체법은 분변의 이물들의 데조염색을 위해 Inns blue를 사용하였으며, 관찰소견으로는 $3-5{\mu\textrm{m}}$의 등근 오오시스트가 9.l은 형광을 띄고 있었고 그외의 주변이물들은 대조염색에 의하여 검붉게 염색되었다 또한 그의 진단율은 현재 일반적으로 사용되고 있는 수입진단킷트(Merifluor, Meridian diagnostic Ins.)와 거의 일치하고 있었다. 이상의 결과를 종합해보면 이번에 생산된 단세포군항체 들은 작은와포자충에 특이적으로 반응하고 있었으며, 이를 이용한 형광항체 진단법은 작은와포자충을 진단하는데 유용하게 사용될 수 있을 것으로 판단된다.

• 한국항행학회논문지
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• 제18권5호
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• pp.421-428
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• 2014

GPS 위성의 궤도는 GPS에서 송신하는 항법메시지를 이용하여 계산할 수 있는데, 본 논문에서는 미국 NGA 정밀궤도력을 실제 궤도로 가정하고 방송궤도력으로 계산한 위성궤도 및 시계와의 차이를 계산하였다. 2004년부터 2013년까지 전 세계와 한반도에서의 궤도오차를 파악하기 위해 한반도에서 관측되는 위성을 별도로 계산하였다. 그 결과 한반도에서 궤도오차가 4 cm, 의사거리 오차가 3 cm 더 작았다. 10년간 GPS 위성의 종류별 궤도오차를 계산하였는데, Block IIA와 IIF의 SISRE 오차가 2.8배 차이가 나는 것을 확인하였다. 위성의 궤도오차와 그림자조건의 상관관계를 분석하였으며 그림자 내부에 있을 때 궤도오차가 2.1% 더 크게 나타났다. 태양활동 및 지자기활동과의 상관관계 분석도 수행하였는데, 2004년부터 2008년까지는 F10.7과 궤도오차가 큰 상관관계를 가지고 있지만 2009년부터 상관관계가 낮아지는 것으로 나타났다.

• Structural Engineering and Mechanics
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• 제45권1호
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• pp.19-32
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• 2013

Cracking at low temperatures is one of the frequently observed modes of failure in asphalt concretes. In this investigation, fracture tests were performed on cracked asphalt concrete subjected to pure mode I and pure mode II loading at different subzero temperatures. An improved semi-circular bend (SCB) specimen containing a vertical crack was used to conduct the experiments. The SCB specimens produced from the gyratory compacted cylindrical samples were compressively loaded, and critical stress intensity factors, $K_{If}$ and $K_{IIf}$, were then calculated using peak loads obtained from the tests. The experimental results showed that with decreasing the temperature, mode I and mode II critical stress intensity factors increased first but below a certain temperature they both decreased. It was also found that at a fixed temperature, the mode II fracture resistance of the asphalt concrete was higher than its mode I fracture resistance.

• 한국통신학회논문지
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• 제10권1호
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• pp.38-41
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• 1985

전단의 IIR필터를 2차계의 pole-zero형으로 취하고 후단의 FIR필터의 필터 길이를 최소화시키는 hybrid필터의 설계를 제시한다. 시뮬레이션 결과 FIR필터의 경우보다 필터 길이가 현저히 줄어 들었고 또한 IIF필터를 all-pole형으로 취했을 때보다도 다소 줄어들었다. Hybrid 필터가 정확한 선형위상(linear phase)특성을 갖지는 못하나 통과대역과 서지대역에서 IIR필터보다는 비교적 정확한 선형특성을 갖게 됨을 알 수 있다.