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Production and diagnostic applications of monoclonal antibodies against porcine circovirus  

Kim, Kyung-Mi (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University)
Jeong, Ji-Hye (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University)
Min, Hong-Ki (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University)
Lee, Seung-Chul (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University)
Roh, In-Soon (National Veterinary Research and Quarantine Service)
Kang, Shien-Young (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University)
Publication Information
Korean Journal of Veterinary Research / v.44, no.2, 2004 , pp. 259-268 More about this Journal
Abstract
Porcine circovirus type 2 (PCV-2) has been associated with various disease in pigs worldwide including postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). In this study, monoclonal antibodies (MAbs) against PCV were produced, characterized and applications of MAbs as diagnostic reagents were described. Spleen or lymph node cells from BALB/c mouse immunized respectively with PCV-1, PCV-2 or expressed PCV-2/ORF2 proteins in baculovirus were fused with SP2/0 myeloma cells using polyethylene glycol (PEG) and hybridoma cells producing PCV-1 or PCV-2-specific antibody were screened by an indirect immunofluorescence (IIF) test. A total of fifteen MAbs were produced against PCV. Six MAbs were PCV-1-specific and nine were PCV-2-specific. All PCV-1-specific MAbs reacted with only PCV-1 and all PCV-2-specific MAbs were reactive with only PCV-2 by IIF test. None of the MAbs was reactive with porcine reproductive and respiratory syndrome virus (PRRSV), porcine parvovirus (PPV), porcine rotavirus (PRV), and transmissible gastroenteritis virus (TGEV). Some PCV-2-specific MAbs recognized the PCV-2 infected porcine tissues by IIF or immunohistochemistry (IHC) assay. From this experiment, it was confirmed that MAbs produced in this study were PCV-specific and could be used as reliable diagnostic reagents for PCV-1/PCV-2 detection and differentiation.
Keywords
porcine circovirus; monoclonal antibody; IIF; IHC;
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