• Title/Summary/Keyword: I3A

Search Result 32,940, Processing Time 0.06 seconds

Moieties of Complement iC3b Recognized by the I-domain of Integrin αXβ2

  • Choi, Jeongsuk;Buyannemekh, Dolgorsuren;Nham, Sang-Uk
    • Molecules and Cells
    • /
    • v.43 no.12
    • /
    • pp.1023-1034
    • /
    • 2020
  • Complement fragment iC3b serves as a major opsonin for facilitating phagocytosis via its interaction with complement receptors CR3 and CR4, also known by their leukocyte integrin family names, αMβ2 and αXβ2, respectively. Although there is general agreement that iC3b binds to the αM and αX I-domains of the respective β2-integrins, much less is known regarding the regions of iC3b contributing to the αX I-domain binding. In this study, using recombinant αX I-domain, as well as recombinant fragments of iC3b as candidate binding partners, we have identified two distinct binding moieties of iC3b for the αX I-domain. They are the C3 convertase-generated N-terminal segment of the C3b α'-chain (α'NT) and the factor I cleavage-generated N-terminal segment in the CUBf region of α-chain. Additionally, we have found that the CUBf segment is a novel binding moiety of iC3b for the αM I-domain. The CUBf segment shows about a 2-fold higher binding activity than the α'NT for αX I-domain. We also have shown the involvement of crucial acidic residues on the iC3b side of the interface and basic residues on the I-domain side.

The Structures of Dehydrated Fully $Ca^{2+}$-Exchanged Zeolite A and of Its iodine Sorption Complex (칼슘 이온으로 완전히 치환된 제올라이트 A의 탈수 구조와 칼슘 치환 제올라이트 A에 요오드가 흡착된 착물 구조)

  • Jang, Se-Bok;Han, Yong-Wook;Kim, Duk-Soo;Kim, Yang
    • Korean Journal of Crystallography
    • /
    • v.1 no.2
    • /
    • pp.76-83
    • /
    • 1990
  • The structures of dehydrated fully Ca2+ _exchanged zeolite A (a: 12.2a3(2) A and of its iodine sorption complex (a=12.258(2) A) have been determined by single-crystal X-ray diffraction methods in the cubic space group. Pm3m at 21(1)℃.the structures were refined to final R(weighted) indices of 0.081 with 206 reflections and 0.084 with 173 reflections, respectively for which I>3 w (I). In each structure. six divalent cations are located on three different theefold axes associated with 6-ring oxygens. Dehydrated Ca2+_A sorbs ca. 6.0 diiodine molecules per unit cell at 80℃(vapor pressure of I: is ca. 14.3 Torr). Each iodine molecule makes a close approach. along its ahs to framework oxygen atom with I-I distance of 2.71(2) A, I-O distance of 3.32(3) k. and I-I-0=180℃. The result indicates that diiodine molecule forms charge transfer complex with framework oxygen.

  • PDF

Changes in the Growth and Quality of Creeping Bentgrass (Agrostis palustris Huds. 'Penn A1') Following Gibberelinic Acid (GA3) Treatment (지베렐린산(GA3) 처리에 따른 크리핑 벤트그래스 (Agrostis palustris Huds. 'Penn A1')의 생장 및 품질 변화)

  • Woo-Sung Kim;Tae-Wooung Kim;Young-Sun Kim;Chi-Hwan Lim
    • Korean Journal of Environmental Agriculture
    • /
    • v.42 no.4
    • /
    • pp.389-395
    • /
    • 2023
  • This study evaluated the effects of gibberellic acid (GA3) on the growth and quality of creeping bentgrass (Agrostis palustris Huds.). Experimental treatments included a No application of fertilizer and GA3 (NFG) Control [3 N active ingredient (a.i.) g/m2], 0.3GA3 (GA3 0.3 a.i. mg/m2/200 mL), 0.6GA3 (GA3 0.6 a.i. mg/m2/200 mL), 1.2GA3 (GA3 1.2 a.i. mg/m2/200 mL), and 2.4GA3 (GA3 2.4 a.i. mg/m2/200 mL). Additionally, the study included a 1.5N+GA3 experiment with similar GA3 treatments combined with 1.5N a.i. g/m2 : NFG, Control (3N a.i. g/m2), 1.5N+ 0.3GA3 (1.5N a.i. g/m2+GA3 0.3 a.i. mg/m2/200 mL), 1.5N+0.6GA3 (1.5N a.i. g/m2+GA3 0.6 a.i. mg/m2/200 mL), 1.5N+1.2GA3 (1.5N a.i. g/m2+GA3 1.2 a.i. mg/m2/ 200 mL), and 1.5N+2.4GA3 (1.5N a.i. g/m2+GA3 2.4 a.i. mg/m2/200 mL). Compared to the NFG, turf color index chlorophyll content was not significantly different (p< 0.05). However, shoot length in 1.2GA3, 2.4GA3, 1.5N+0.3GA3, 1.5N+0.6GA3, 1.5N+1.2GA3, and 1.5N+2.4GA3 treatments increased by 0.8%, 10.6%, 5.15%, 8.3%, 13.5 %, and 21.6%, respectively, compared to the control. As compared to the control, clipping yield in 1.5N+1.2GA3 and 1.5N+2.4GA3 treatments increased by 7.1% and 14.3 %, respectively. These results indicated that GA3 application increased shoot length, with the 1.2GA3 treatment showing shoot length similar to the control (3N a.i. g /m2 ).

Effect of Microstructures and Sintering Additives on the Mechanical Properties of Si$_3$N$_4$ (질화규소의 기계적 특성에 미치는 미세구조 및 소결조제의 영향)

  • Park, Hye-Ryeon;Lee, O-Sang;Park, Hui-Dong;Lee, Jae-Do
    • Korean Journal of Materials Research
    • /
    • v.2 no.5
    • /
    • pp.330-336
    • /
    • 1992
  • Four distinctive hot pressed and heat treated S${i_3}{N_4}$ceramics, S${i_3}{N_4}$-8%${Y_2}{O_3}$, S${i_3}{N_4}$-6% ${Y_2}{O_3}$-2% $A{l_2}{O_3}$, S${i_3}{N_4}$-4% ${Y_2}{O_3}$-3% $A{l_2}{O_3}$, 그리고 S${i_3}{N_4}$-1% MgO-1% Si$O_2$(in wt%), were prepared and characterized by X-ray diffraction, scanning electron microscopy, image analysis and mechanical tests. The fracture toughness of S${i_3}{N_4}$-8% ${Y_2}{O_3}$specimens containing large elongated grains showed the highest value of about 9.8MPa$m^{1/2}$. Two out of four S${i_3}{N_4}$, ceramics(S${i_3}{N_4}$-6% ${Y_2}{O_3}$-2% $A{l_2}{O_3}$and S${i_3}{N_4}$-4% ${Y_2}{O_3}$-3% $A{l_2}{O_3}$) heat treated at 200 $0^{\circ}C$retained the fracture strength of over 900MPa and fracture toughness of over 8.0MPa$m^{1/2}$. Large ${\beta}$-S${i_3}{N_4}$grains having a diameter larger than 1${\mu}$m appeared to contribute to increase in fracture toughness.

  • PDF

SOME RESULTS ON 1-ABSORBING PRIMARY AND WEAKLY 1-ABSORBING PRIMARY IDEALS OF COMMUTATIVE RINGS

  • Nikandish, Reza;Nikmehr, Mohammad Javad;Yassine, Ali
    • Bulletin of the Korean Mathematical Society
    • /
    • v.58 no.5
    • /
    • pp.1069-1078
    • /
    • 2021
  • Let R be a commutative ring with identity. A proper ideal I of R is called 1-absorbing primary ([4]) if for all nonunit a, b, c ∈ R such that abc ∈ I, then either ab ∈ I or c ∈ ${\sqrt{1}}$. The concept of 1-absorbing primary ideals in a polynomial ring, in a PID and in idealization of a module is studied. Moreover, we introduce weakly 1-absorbing primary ideals which are generalization of weakly prime ideals and 1-absorbing primary ideals. A proper ideal I of R is called weakly 1-absorbing primary if for all nonunit a, b, c ∈ R such that 0 ≠ abc ∈ I, then either ab ∈ I or c ∈ ${\sqrt{1}}$. Some properties of weakly 1-absorbing primary ideals are investigated. For instance, weakly 1-absorbing primary ideals in decomposable rings are characterized. Among other things, it is proved that if I is a weakly 1-absorbing primary ideal of a ring R and 0 ≠ I1I2I3 ⊆ I for some ideals I1, I2, I3 of R such that I is free triple-zero with respect to I1I2I3, then I1I2 ⊆ I or I3 ⊆ I.

Crystal Structures of Vacuum Dehydrated Fully $Cd^{2+}$-Exchanged Zeolite A and of Its iodine Sorption Complex (카드뮴 이온으로 완전히 치환한 제올라이트 A를 진공 탈수한 구조와 이것에 요오드를 흡착한 결정구조)

  • Jang, Se-Bok;Han, Young-Wook;Kim, Yang
    • Korean Journal of Crystallography
    • /
    • v.4 no.2
    • /
    • pp.54-62
    • /
    • 1993
  • The crystal shnture of dehydrated fully Cd2+-exchanged zeolite A evacuated at 2 × 10-6 Torr and 650℃ (a:12.189(2) A) and of its iodine sorption corrplex (a:12.168(2)A) have been netsmlmn by single uystal x-ray diffraction techliques in the cubic space group hkTn at 21(1)℃. The strutures were refined to final error indices, Ri:0.057 and R2 =0.063 with 186 reflections and Rl:0.082 and R2:0.085 with 181 reflections, respectively, for which 1>3σ(In both structure, six lie at two distinguished threefold axes of unit cell ten the crystal structure of an iodine sorption complex of Cd6-A four Cd2+ ions are recessed 0.69(1) A into the large cavity to complex each with from the (111) plane of 0(3), whereas two Cd2+ ions recessed 0.68(1) A into the sodalite unit Awximately 4.0 l3ions per nit cell are sorbed. Each bridge between a Cd2+ ion and 8-ring oxygens ((I-I-I)= 117(1) ˚ and 0(1)-I(1)-I(2)=172(1)). The near linear I-I-0 angle and its interatomic distance (I-0=3.57(3) A) are indicative of a weak charge transfer interacticn between the frarrework oxygen and iodine. The existence of In3 inside the large cavity indicates that the If ions and H ions may be produced by reaction of In vapor with water molecules which maybe associated with Cd2+ ions in partially dehydrated Cd6-A In3- ions may be produced by the combination of I- and I2.

  • PDF

The Effect of Insulin-Like Growth Factor-I(IGF-I) and IGF Binding Protein-3(IGFBP-3) on Cellular Proliferation in Mouse 3T3 Fibroblast Cells (마우스 섬유아세포(3T3 fibroblast cells)에서 Insulin-like Growth Factor-I(IGF-I) 및 IGF Binding Protein-3 (IGFBP-3)이 세포증식에 미치는 영향)

  • Cho, Chul-Ho;Kwak, Seung-Min;Moon, Tae-Hun;Cho, Jae-Hwa;Ryu, Jeong-Seon;Lee, Hyong-Lyeol
    • Tuberculosis and Respiratory Diseases
    • /
    • v.47 no.5
    • /
    • pp.618-628
    • /
    • 1999
  • Background: Cell growth is a balance between cell proliferation and cell death. Insulin-like growth factor-I(IGF-I), which binds IGF-I receptor(IGF-IR), mediates cellular proliferation as a potent mitogen. IGF binding protein-3(IGFBP-3) as a circulating major IGFBP can inhibit or enhance the effects of IGF-I on cellular growth by binding IGFs. Methods: We investigated the expressions of mRNA of IGF-I and IGF-IR by northern blot and phosphorylation of IGF-IR with the treatment of IGF-I by western blot in 3T3 fibroblast cells. The cellular proliferations of 3T3 cells with the treatments of IGF-I were evaluated using $^3H$-thymidine incorporation and MTT assay. Also to observe the effect of IGFBP-3 on cellular proliferation, 3T3 cells were treated with anti-IGFBP-3 and ${\alpha}IR_3$(monoclonal antibody to IGF-IR) alone or in combination. Results: Our results demonstrated that 3T3 cells showed mRNA expressions of IGF-I and IGF-IR and the IGF-I increased phosphorylation of IGF-IR. The treatments of 3T3 cells with IGF-I increased cellular proliferation in 5 % and 1 % seruma-containing media, not in serum-free media. The addition of anti-IGFBP-3 to neutralize IGFBP-3 showed 2-fold increase of cellular proliferation, and also co-incubation of anti-IGFBP-3 and ${\alpha}IR_3$ together showed similar increase of cellular proliferation in 3T3 cells. Interestingly, when the cells were pretreated with ${\alpha}IR_3$ for 4 hr, prior to the simultaneous addition of ${\alpha}IR_3$ and anti-IGFBP-3, anti-IGFBP-3-mediated cellular proliferation was decreased to control level. All of these results suggest that free IGF-I released from IGF-I/IGFBP-3 complex would be involved in the cellular proliferation. Conclusion: IGF-I is a mitogen through the activation of IGF-IR in 3T3 cells, and IGFBP-3 could be a potent inhibitor for IGF-I action by binding IGF-I.

  • PDF

Three ORF-Containing Group I Introns in Chloroplast SSU of Caulerpa sertularioides (Ulvophyceae) and Their Evolutionary Implications

  • Lee, Jung-Ho;Manhart, James R.
    • ALGAE
    • /
    • v.18 no.3
    • /
    • pp.183-190
    • /
    • 2003
  • Except for a group I intron in trnL-uaa occuring in eubacteria and plastids, group I introns are rarely documented in plastid genomes. Here, we report that a green alga, Caulerpa sertularioides, contains three group IA3 introns in the 16S gene (cpSSU), CS-cpSSU.i1, CS-cpSSU.i2 and CS-cpSSU.i3. Each intron has an open reading frame with LAGLIDADG motifs. CS-cpSSU.i1orf and CS-cpSSU.i3orf occur at Loop 6 in the intron secondary structure and CScpSSU. i2orf at Loop 8. CS-cpSSU.i1orf and CS-cpSSU.i2orf contain both LAGLI-DADG motifs but CS-cpSSU.i3orf has only one. CS-cpSSU.i1 and CS-cpSSU.i2 share the insetion sites and the ORFs at Loop 6 and 8 with CpSSU·1 and CpSSU·2 introns of Chlamydomonas pallidostigmatica (Chlorophyceae). In contrast, CS-cpSSU.i3, containing 28 copies of GAAATAT at Loop 6, is a novel intron found only in Caulerpa sertularioides. Possible scenarios of the evolution of the three introns and their possible use in systematic research are discussed.

Numerical Solution for Nonlinear Klein-Gordon Equation by Using Lagrange Polynomial Interpolation with a Trick (라그란제 보간을 사용한 비선형 클라인 고든 미분방적식의 수치해)

  • Lee In-Jung
    • The KIPS Transactions:PartA
    • /
    • v.11A no.7 s.91
    • /
    • pp.571-576
    • /
    • 2004
  • In this paper, by using Lagrange polynomial interpolation with a trick such that for $f(x)^{3}$ we shall use $f(x_i)^{3}I_i(x)^{3}$ instead of $I(x)^{3}$ where $I{x}{\;}={\;}\sum_{i}^{f}(x_i)I_i(x)$. We show the convergence and stability and calculate errors. These errors are approximately less than $C(\frac{1}{N})^{N-1} hN(N-1)(\frac{N}{2})^{N-1} /(\frac{N}{2})!$ where N is a polynomial degree.

The Crystal Structure of an Iondine Sorption Complex of Dehydrated Calcium and Silver Exchanged Zeolite A ($Ag^+$이온과 $Ca^{2+}$이온으로 치환한 제올라이트 A를 탈수한 후 요오드를 흡착한 결정구조)

  • Bae, Myung-Nam;Kim, Yang;Kim, Un-Sik
    • Korean Journal of Crystallography
    • /
    • v.6 no.2
    • /
    • pp.118-124
    • /
    • 1995
  • The crystal structure of an iodine sorption complex of vacumm-dehydrated Ag+ and Ca2+ exchanged zeolite A(a=12.174(3)Å) has been determined at 21℃ by single-crystal X-ray diffraction techniques in the cubic space group Pm3m. The crystal was prepared by flow method for three days using exchange solution in solution in which mole ratio of AgNO3 and Ca(NO3)2 was 1:150 with total concentration of 0.05 M. The complex was prepared by dehydration at 360℃ and 2×10-6 Torr for 2 days, followed by exposure to about 14.3 Torr of iodine vaporat 80℃ for 24 hours. Full-matrix least-squares refinement converged to the final error indices of R1=0.082, R2=0.068 using 122 reflections for which I > 3σ(I). Two Ag+ ions, 1.1 Ag+ ions, and 4.45 Ca2+ ions per unit cell are located on three different three-fold axes associated with 6-ring oxygens. Two Ag+ ions per unit cell are in the large cavity, 1.399(4)Å from the (111) plane of three oxygens. Another 1.1 Ag+ ions are found at opposite sites. Six iodine molecules are sorbed per unit cell. Each I2 molecule approaches a framework oxide ion axially (O-I=3.43(2)Å, I-I=2.92Å, I-I-O;166.1(3)°), by a charge transfer complex interaction. Two Ag+ ions make a close approach to the iodine molecules (Ag-I ; 2.73(2)Å).

  • PDF