• Journal of Bio-Environment Control
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• v.24 no.1
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• pp.45-50
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• 2015

Edible sprouts are highly nutritious, containing numerous health-promoting phytochemicals and nutrients. However, the process of sprouting is subject to potential contamination by microorganisms attached to the seed coat. The objective of this study was to determine the effects of air anions generated by spray ionization on growth and sterilization of edible sprouts. Treatment with air anions has positive effects on plant growth and sterilization in 4 kinds of sprouts. Hypocotyl length in red cabbage and kale increased approximately 1.26-fold and radicle length of lettuce, red cabbage, and kale increased 1.4 to 1.6-fold compared to the control. The fresh weight of sprouts in the spray-ionization treatment was significantly (16.0-38.5%) higher than that of the controls. Radicle activity in sprouts exposed to anions was higher than that of the control in all species. Bacterial counts on lettuce, red cabbage, and tatsoi decreased significantly (by 41%, 66%, and 19%, respectively), and bacterial colonies also declined in drainage water. Our findings suggest that spray-ionization treatment is useful for improving sprout growth and for sterilizing sprouts.

• Korean Journal of Breeding Science
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• v.49 no.3
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• pp.230-234
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• 2017

A mungbean variety 'Suhyeon' (Vigna radiata (L.) Wilczek) was derived from the cross between 'Jeonnam14' and 'IT208777' at the Jeollanamdo Agricultural Research and Extension Services (JARES) in 2010. 'Suhyeon' has erect growth habit, heart leaflet, green hypocotyl, black and straight pod when matured, and dull green seed surface. The stem length of 'Suhyeon' was 66 cm, which was 5 cm less than check variety 'Owool'. The pods number per plant of 'Suhyeon' was 24, which was 5 more than check variety. The sprout yield ratio of 'Suhyeon' was 8.67 times, which was 16% more than check variety. The contents of starch, protein and fatty acid of seed were 62.7, 27.3, 1.89%, respectively. The average yield of 'Suhyeon' was 1.74 ton/㏊, which increased 8% more than that of check variety. (Variety registration number: 5107)

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.427-431
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• 2002

Lateral roots of soybean sprout might reduce the quality. The study was done to measure the effect of light quality treated during 24 hour imbibition or 6 day culture on growth and development of soybean sprouts on the 6th day after culture. With the soybean seeds imbibed in 4 ppm benzyladenopurine (BA) solution for last 6 hours of the imbibition, blue and red lights were treated during the imbibition, but during 6 day culture, blue and red or far-red light treatments were done for 50 minutes or 5 hours a day, respectively, the periods taking for their cotyledons to turn green color, On the 6th day after culture, the soybean sprouts were classified by 4 categories on the base of hypocotyl length;＞7cm, 4 to 7cm, ＜4cm and non-germination, and their lateral roots, hypocotyl diameters and fraction dry weights were measured. Blue and red lights treated during the imbibition completely blocked lateral root formation regardless of the lights treated during the culture, and showed nearly the same rate of hypocotyls of longer than 4cm. The period of each light treatment forced during the culture did not influence the growth of soybean sprouts. far-red light treated for 5 hours everyday, however, had the least rate of seed germination and hypocotyls of longer than 7cm of the light quality treatments. In addition, red and far-red lights almost equally having the commercial soybean sprouts of longer than 4cm hypocotyls move elongated and selenderized than blue light and dark treatment, meaning the growth and morphology of soybean sprouts was affected by light treatments during the culture.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.4
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• pp.615-620
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• 2002

This study was conducted to elucidate the characteristics of soybean sprouts cultured in water containing germanium. In three varieties of Eunhakong, Seomoktae and Subaktae, the yields of germanium soybean sprout were 10~20% more than those of control soybean sprouts after 5 day-cultivation. The hypocotyl of germanium soybean sprouts was thicker than that of control soybean sprouts, but there were no significant differences in the length of hypocotyl and the thickness of cotyledon. Germanium soybean sprouts showed deeper green and yellow color than control soybean sprouts. In addition, the hardness exhibited strongly in germanium soybean sprouts, especially Seomoktae. In the sensory evaluation, germanium soybean sprouts were better than control soybean sprouts in appearance, taste, flavor and preference.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.2
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• pp.119-124
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• 2008

Storage conditions of selling mungbean sprouts should affect their marketability. The study was done to examine the effect of light quality (blue, green, yellow, red, far-red) on greening period, shape, fresh and dry weights of mungbean (cv. Zhong Lu 1) sprouts for 5 days to get some information on designing their various polyethylene envelops with different colors. Periods taking to green were 2 to 3.5 days in blue, red and far-red lights but longer than 6 days in green and yellow lights. Yellow lights had the longest total length adding hypocotyl and root, and blue light did the widest hypocotyl diameter while all light treatments showed similar lateral roots for the period. Total fresh and dry weights were the highest in red light but the least in far-red light. Their lengths and weights in green and yellow lights showing the longest periods for their greening declined with increased storage periods but showed no difference between them, meaning that the envelops should be designed mainly by green and yellow colors.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.143-153
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• 2016

Plant regeneration via organogenesis and somatic embryogenesis was investigated in Korean soybean cultivars including Cheongja 3, Jinpumkong 2, Taekwangkong and Uram. Cotyledon, cotyledon+hypocotyl and hypocotyl segments of 7-day-old seedlings were cultured on MS medium containing various concentration (0, 1, 2 and 4 ㎎/L) of BA and TDZ. The results showed that MS medium supplemented with BA 2.0 ㎎/L yielded the highest shoot formation ratio of 83.3%. In 4 cultivars, Taekwangkong showed the highest ratio of shoot formation. When various sizes of immature cotyledons (S: 1∼ 2 ㎜, M: 3∼5 ㎜, L: 6∼8 ㎜) were tested on MS medium containing 2,4-D 40 ㎎/L for somatic embryogenesis, the optimum size for embryogenic callus induction was 3∼5 ㎜ in length of immature cotyledons. In 4 cultivars, Taekwangkong showed the highest percentage of embryogenic callus induction. The results indicate that Taekwangkong is the best soybean cultivar for plant regeneration via organogenesis and embryogenic callus induction among the 4 cultivars.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.477-481
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• 2004

In bean sprout culture water imbibition and benzyladenopurine (BA) treatment are done at the same time. The study was carried out to check the effect of treatment period (3, 5, and 7 hours) on growth of mungbean (cv. Keumseongnogdu, Owoolnogdu, and Zhong Lu 1) sprouts and to analyse its absorption amount on the base of their moisture content. The 3 cultivar seeds were soaked in 50 ppm BA solution immediately before 3 hour aeration and then cultured for 6 days. The sprouts were sorted by 4 categories on the base of hypocotyl length; > 7cm, 4 to 7cm < 4cm, and non-germination, and their morphological characters, fresh and dry weights were measured. The cultivar Zhong Lu 1 had the highest rate in longer than 7cm hypocotyls of the three cultivars but the lowest one in shorter than 4cm. Rates of the above 4 categories in cv. Keumseongnogdu, Owoolnogdu showed no significant difference between the treatment periods while one of longer than 7cm hypocotyls in cv. Zhong Lu1 was decreased with longer treatment period. Lateral roots were less formed with longer treatment period, especially as lengthened from 3 to 5 hours. Hypocotyl and root were also lengthened with longer treatment period and hypocotyl was more thickened in 5 hour treatment period than in the two others. Total fresh and dry weights per sprout showed no significant difference between treatment periods although cv. Zhong Lu1 relatively faster grew than the other cultivars. In the case of shorter than 5hour treatment periods the absorption amount of BA was the greatest in cv. Zhong Lu1 but in 7 hour treatment period it was the greatest in cv. Keumseongnogdu and Zhong Lu 1.

• Korean Journal of Plant Resources
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• v.25 no.2
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• pp.277-284
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• 2012

In the present study, the effects of plant growth regulators on adventitious shoot and root formation of various explants of $in$ $vitro$ seedlings of $Solanum$ $nigrum$ L. were investigated to determine the optimum conditions for the high-efficiency plant regeneration of this species. The formation of adventitious shoots was higher in leaf explants than in cotyledon, hypocotyl, or epicotyl explants at low concentrations (0.5~2.0 mg $L^{-1}$ ) of 6-benzylaminopurine (BAP). The number of adventitious shoots and the shoot length were also higher in both leaf and cotyledon explants. In particular, 2.0 mg $L^{-1}$ BAP was most effective for stimulating the induction and multiplication of adventitious shoots. In terms of root formation and root development from shoots that were separated from multiple shoots, indole butyric acid (IBA) and indole acetic acid (IAA) were more effective than ${\alpha}$-naphthalene acetic acid (NAA). The percentage of rooting as well as the number of roots per shoot (4.0), root length (7.82 cm), and shoot length (8.76 cm) was highest on MS media supplemented with 0.05 mg $L^{-1}$ IAA. Furthermore, 100% of the regenerated plantlets survived when transplanted to compost soil. These results suggest that leaf explants are the best source for the high-efficiency regeneration of $S.$ $nigrum$ L., and that 2.0 mg $L^{-1}$ BAP and 0.05 mg $L^{-1}$ IAA are the best conditions for shoot and root induction, respectively.

• Journal of Bio-Environment Control
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• v.11 no.1
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• pp.23-28
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• 2002

This experiment was conducted far the efficient use of plant growth retardants (PGR) for suppression of plug seedling height. 'Seokwang' tomato seeds were soaked in I5 mL solution of daminozide (1,000, 10,000, or 100,000 mg.L-1) or uniconazole (1, 10, or 100 mg.L-1). And then, they were put in $25^{\circ}C$ chamber for one or three days. Seeds were washed in tap waters and were dried in 5$^{\circ}C$ chamber for one day. Dried tomato seeds were sown and seedlings were raised in 288-cell plug trays. Seedlings grown were evaluated at 21 and 38 days after sowing. Treatments of 10 or 100 mg.L-1 uniconazole as compared to the control had significantly suppressed hypocotyl length and seedling height, but fresh and dry weights of roots, emergence, no. of leaves, chlorophyll concentration, dry matter, and T/R ratio were not significant by different among treatments.

• Current Research on Agriculture and Life Sciences
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• v.17
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• pp.7-13
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• 1999

Thirty bacteria were isolated from the red pepper rhizosphere. The isolates were screened for antagonism to Alternaria alteranta causing red pepper black rot. Antagonistic bacterium No. J-24 was selected among the isolated bacteria and was identified as Bacillus subtilis based on morphological and physiological characteristics and MIDI system. B. subtilis J-24 showed antifungal activities against A. alternata(inhibition percentage, 99%), Botrytis cinerea, Phytophthora capsici, Pythium ultimum, Colletotrichum gloeosporioides, Stemphylium botryosum. The growth of red pepper seedling was promoted as compared to control when the microbial inoculants was mixed in bed soil. In the mixed microbial inoculants bed soil, the leaf area of red pepper was increased of 15 percent, the hypocotyl weight 12 percent, the root length 12 percent, total dry weight 13 percent as compared to those grown in the general bed soil.