• 한국전기전자재료학회논문지
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• 제17권7호
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• pp.729-737
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• 2004

In this paper, a DNA chip with a microelectrode array was fabricated using microfabrication technology. Several probe DNAs consisting of mercaptohexyl moiety at their 5 end were immobilized on the gold electrodes by DNA arrayer. Then target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Linear sweep voltammetry or cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. It was derived from Hoechst 33258 concentrated at the electrode surface through association with formed hybrid. It suggested that this DNA chip could recognize the sequence specific genes.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2006년도 하계학술대회 논문집 Vol.7
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• pp.372-373
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• 2006

In this paper, a DNA chip with a microelectrode array was fabricated using microfabrication technology. Several probe DNAs consisting of mercaptohexyl moiety at their 5 end were immobilized on the gold electrodes by DNA arrayer. Then target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Linear sweep voltammetry or cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. It was derived from Hoechst 33258 concentrated at the electrode surface through association with formed hybrid. It suggested that this DNA chip could recognize the sequence specific genes.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2012년도 춘계학술대회 논문집
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• pp.439-440
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• 2012

New analysis methods complement classical approaches in the vehicle NVH development by reducing and accelerating iteration steps to obtain a target sound. Therefore, tools are required that allow an integrative approach of sound engineering and structural analysis and enable a precise simulation and modification based on measured data. The Response Modification Analysis (RMA) is such a hybrid solution, which provides indications of relevant transfer paths taking into account the sensitivity of response channels to modifications of reference channels.

• 미생물과산업
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• 제18권3호
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• pp.53-62
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• 1992

최근들어 새로운 항생물질을 발견하기 위한 연구 동향은 크게 세가지로 대별된다. 첫째는 Streptomyces 속을 제외한 희귀 방선균류를 분리하거나 해양 미생물과 버섯류 및 곰팡이를 대상으로 하여 새로운 항생 물질의 발견 가능성을 증대시키려는 것과 둘째는 기존의 무작위 탐색방법을 탈피하고 보다 선택적이고 특이적인 목표 지향적인 탐색(target directed screening)을 통해 선별적인 탐색을 행하고 셋째는 유전자 조작을 통해 hybrid 항생물질을 생산하기 위한 여러 시도로 나눌수 있다. 한편 방선균의 가장 중요한 경제적인 측면은 항생물질 생산이라고 일컬어지고 있으나 방선균은 항생물질 이외에도 다른 상업적인 제품의 공급원이 되고 있으며, 어떤 방선균들은 한 종류 이상의 항생물질을 생산하기 때문에 새로운 발효기술을 응용하면 새로운 물질을 발견하는데 매우 커다란 도움을 줄 것으로 생각된다.

• International Journal of Advanced Culture Technology
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• 제9권1호
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• pp.121-128
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• 2021

In this study, we aim to suggest direction for landscape design of residential complex in Korea by studying target areas to understand how landscape urbanism was applied to residential landscape design as it is the majority of landscape design in Korea and by closely examining design elements to be considered for design of residential complex based on landscape urbanism theory. For the purpose of the study, some of public multifamily housing complex were selected as target areas and a survey was first conducted to understand how landscape urbanism was applied to design of these target areas. Then, by analyzing the survey results, we identified which elements were of importance to designing of multifamily housing complexes in Korea and how practical design of landscape urbanism works. From this study, we concluded that landscape urbanism theory is not just about design strategies, but more of comprehensive design methodology covering both operation and management and that such theory can be appropriately applied to design of multi-housing complexes in Korea. We also discovered that in order to apply landscape urbanism in design stage for multi-housing complex, selecting which design strategy/words in the previous study is not what's important, but comprehensive understanding and interpretation of design languages is key.

• 한국산학기술학회논문지
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• 제19권3호
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• pp.520-534
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• 2018

본 연구는 건강 불평등의 개념을 정립하고 정의하기 위해 시행되었다. 연구 분석 방법은 Schwartz-Barcott와 Kim의 혼종 모형(Hybrid model)을 이용하였으며, 자료 수집은 14명의 대상자와 건강 불평등과 관련된 문헌으로부터 수집하였다. 본 연구 결과, 건강 불평등은 3개의 차원과 9개의 속성으로 도출되었으며, 3개의 차원은 대상영역, 선행영역, 결과영역 이었다. 대상 영역은 건강 불평등의 대상이 되는 영역, 선행 영역은 건강 불평등을 일으키는 원인이 되는 영역, 결과 영역은 건강 불평등의 결과가 되는 영역을 말한다. 즉, 건강 불평등의 정의는 개인, 가족, 지역사회 또는 사회적, 경제적, 지리적으로 구분되는 인구집단이 (i) 병원에서의 홀대받음, (ii) 떼우는 끼니, (iii) 일을 할 수 밖에 없는 현실, (iv) 질병관리의 의료비 부담, (v) 지역 간 의료의 질적 차이, (ⅵ) 질병의 지식부족, (ⅶ) 묶여있는 시간으로 인한 부적절한 건강 관리와 같은 건강 불평등으로 인하여 병을 얻게 되거나 병을 키우게 되며, 생존률과 삶의 질이 감소하게 되어 결과적으로 인간의 기본권이 침해되는 것을 의미 한다. 본 연구는 건강 불평등의 개념을 정립함으로 인해 건강불평등을 감소시킬 수 있는 전략에 실제적인 밑거름이 될 수 있는 자료를 제공했다는데 의의가 있으며 본 연구를 기초로 하여 건강 불평등을 줄이기 위한 프로그램 개발을 제안한다.

• Journal of Plant Biotechnology
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• 제48권1호
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• pp.34-43
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• 2021

CRISPR/Cas9에 의한 유전자 교정 기술은 유용 형질을 갖는 작물 및 임목의 육성에 있어 널리 사용되고 있는 핵심 기술이다. 유전자 교정 임목 육성에는 아그로박테리움에 의한 형질전환 방법이 높은 효율로 시행된 연구가 많았고 따라서 형질전환에 사용된 플라스미드 서열이 식물 유전체 안에 존재한다는 문제가 남아 있었다. 본 연구에서는 CRISPR/Cas9을 사용하여 유전자 교정 임목을 육성하는 데 기존에 알려진 벡터 도입 기술이 아닌, 단일 가닥 가이드 RNA (sgRNA)와 Cas9 단백질을 혼합하여 만든 리보핵산단백질을 현사시나무 원형질체에 도입하는 방법을 기술하였다. 염 스트레스 내성 관련 인자 PagSAP1 유전자를 표적으로 하는 3종류의 sgRNA를 디자인하고, 각 sgRNA와 Cas9 단백질을 혼합하여 만든 리보핵산단백질을 원형질체에 도입하였다. 표적화 딥시퀀싱을 통해 리보핵산단백질 형성 시 sgRNA와 Cas9 단백질을 혼합하고 일정 시간 배양하여 안정화되는 시간이 필요한 것을 확인하였다. 또한 sgRNA3의 리보핵산단백질이 sgRNA1, sgRNA2의 리보핵산단백질보다 높은 교정 효율을 보이는 것을 확인하였다. 본 실험을 통해 리보핵산단백질을 이용한 유전자 교정 기술이 임목에도 적용될 수 있음이 확인되었고, 이는 외래 유전자 없이 유전자 교정 임목을 육성하는 데 활용할 수 있을 것으로 사료된다.

• 한국자동차공학회논문집
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• 제21권6호
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• pp.92-99
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• 2013

High oil price and global warming problem are being continued all over the world. For this reason, fuel economy and emission of greenhouse gas are regulated by law in many countries. Therefore many companies are researching and producing hybrid electric vehicles (HEVs) which substitute conventional internal combustion engine vehicle. However, these researches and productions are restricted to mainly passenger cars. Because of cost and physical problems, commercial vehicles are difficult to evaluate fuel economy. So simulations are important and it is necessary to know how sensitive parameters that enter into simulation affect. In this paper, forward simulations using AVL Cruise were conducted for analysis of fuel economy for parallel hybrid bus and were repeated by changing each parameter. Based on these results, root mean square errors (RMSE) are calculated for analysis of fuel economy sensitivity. The number of target parameters are 15. These parameters were classified with high and low sensitivity parameter relatively.

• Bulletin of the Korean Chemical Society
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• 제34권3호
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• pp.735-742
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• 2013

Peptide nucleic acids (PNAs) that bind to complementary nucleic acid sequences with extraordinarily high affinity and sequence specificity can be used as antisense oligonucleotides against microRNAs, namely antagomir PNAs. However, methods for efficient cellular delivery must be developed for effective use of PNAs as therapeutic agents. Here, we demonstrate that antagomir PNAs can be delivered to hepatic cells by complementary DNA oligonucleotide and cationic liposomes containing galactosylated ceramide and a novel cationic lipid, DMKE (O,O'-dimyristyl-N-lysyl glutamate), through glycoprotein-mediated endocytosis. An antagomir PNA was designed to target miR-122, which is required for translation of the hepatitis C virus (HCV) genome in hepatocytes, and was hybridized to a DNA oligonucleotide for complexation with cationic liposome. The PNA-DNA hybrid molecules were efficiently internalized into hepatic cells by complexing with the galactosylated cationic liposome in vitro. Galactosylation of liposome significantly enhanced both lipoplex cell binding and PNA delivery to the hepatic cells. After 4-h incubation with galactosylated lipoplexes, PNAs were efficiently delivered into hepatic cells and HCV genome translation was suppressed more than 70% through sequestration of miR-122 in cytoplasm. PNAs were readily released from the PNA-DNA hybrid in the low pH environment of the endosome. The present study indicates that transfection of PNA-DNA hybrid molecules using galactosylated cationic liposomes can be used as an efficient non-viral carrier for antagomir PNAs targeted to hepatocytes.

• 한국표면공학회지
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• 제43권5호
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• pp.238-242
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• 2010

In the present work, the influence of oxide on the Cr-Si-N coatings was investigated for the Cr-Si-O-N coatings on AISI 304 and Si wafer deposited by hybrid system, which combines the DC magnetron sputtering technique and arc ion plating (AIP) using Cr and Si target in an $Ar/N_2/O_2$ gaseous mixture. As the O content in the Cr-Si-N coatings increased, the diffraction patterns of the Cr-Si-O-N coatings showed CrN and $Cr_2O_3$ phases. However, as the O content increased to 28.8 at.%, diffraction peak of $Cr_2O_3$ was disappeared in the Cr-Si-O-N coating. The $d_{200}$ value was decreased with increasing of O content. The average grain size increased from about 40 nm to 65 nm as the O content increased. The maximum micro-hardness of the Cr-Si-O-N coating was obtained 4507 Hk at the O content of 24.8 at.%. The average friction coefficient of the Cr-Si-O-N coatings was gradually decreased by increasing the O content and the average friction coefficient decreased from 0.37 to 0.25 by increasing the O content. These results indicated that amorphous phase was increased in the Cr-Si-O-N coatings by increasing of O content.