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http://dx.doi.org/10.4313/JKEM.2004.17.7.729

Eletrochemical Detection of Gene using Microelectrode-array DNA Chip  

Eiichi Tamiya (Japan Advanced Institute of Science and Technology, School of Materials Scienc)
Publication Information
Journal of the Korean Institute of Electrical and Electronic Material Engineers / v.17, no.7, 2004 , pp. 729-737 More about this Journal
Abstract
In this paper, a DNA chip with a microelectrode array was fabricated using microfabrication technology. Several probe DNAs consisting of mercaptohexyl moiety at their 5 end were immobilized on the gold electrodes by DNA arrayer. Then target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Linear sweep voltammetry or cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. It was derived from Hoechst 33258 concentrated at the electrode surface through association with formed hybrid. It suggested that this DNA chip could recognize the sequence specific genes.
Keywords
DNA chip microarray; Microfabrication technology; Hoechst 33258; Linear sweep voltammetry; Cyclic voltammetry; Cyclic voltammetry;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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