• Mycobiology
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• v.49 no.6
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• pp.589-598
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• 2021

White strains of Hypsizygus marmoreus are more difficult to cultivate than are brown strains; therefore, new white strain breeding strategies are required. Accordingly, we constructed the genetic map of H. marmoreus with 1996 SNP markers on 11 linkage groups (LGs) spanning 1380.49 cM. Prior to analysis, 82 backcrossed strains (HM8 lines) were generated by mating between KMCC03106-31 and the progenies of the F1 hybrid (Hami-18 × KMCC03106-93). Using HM8, the first 23 quantitative trait loci (QTLs) of yield-related traits were detected with high limit of detection (LOD) scores (1.98-9.86). The length, thickness, and hardness of the stipe were colocated on LG 1. Especially, length of stipe and thickness of stipe were highly correlated given that the correlation coefficients were negative (-0.39, p value ≤ .01). And a typical biomodal distribution was observed for lightness of the pileus and the lightness of the pileus trait belonged to the LG 8, as did traits of earliness and mycelial growth in potato dextrose agar (PDA) medium. Therefore, results for color traits can be suggested that color is controlled by a multi-gene of one locus. The yield trait was highly negatively correlated with the traits for thickness of the stipe (-0.45, p value ≤ .01). Based on additive effects, the white strain was confirmed as recessive; however, traits of mycelial growth, lightness, and quality were inherited by backcrossed HM8 lines. This new genetic map, finely mapped QTLs, and the strong selection markers could be used in molecular breeding of H. marmoreus.

• Korean Journal of Microbiology
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• v.31 no.1
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• pp.1-8
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• 1993

lntergeneric hybrids formed between Aspergillus niger and Penicillium verruculosum were obtained by nuclear transfer technique. Nuclei isolated from wild type and auxotrophic mutants of donor strains were transferred into the protoplasts of different auxotrophic mutants as recipient strains. Several auxotrophic mutants were isolated from conidiospores of the two strains mutagenized with ultraviolet and N-methyl-N'-nitrosoguanidine. Optimal conditions for formation of intergeneric hybrids were investigated. Frequencies of intergeneric hybrid formation by nuclear transfer were $7{\times}10^{5}~1{\times}10^{5}$. From observations of genetic stability. DNA content. nuclear stain and conidial size. it was suggested that their karyotypes are aneuploid. In addition. the hybrids possess the 1.1~2.3-fold higher cellulase activities than those of parental strains. It was also revealed that some hybrids had different isozyme patterns compared to those of parental strains by CMCase and $\beta$-glucosidase activity assays.

• The Korean Journal of Mycology
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• v.49 no.4
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• pp.507-514
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• 2021

Lentinula edodes have been cultivated extensively not only in South Korea but also in other Asian countries. In terms of taste and nutrition, it is a valuable mushroom that is comparable to other mushrooms. L. edodes contains various physiologically active compounds that promote human health. One compound, ergothioneine, exerts a powerful antioxidant function that inhibits cellular senescence. As it is not biosynthesized by plants or animals, L. edodes is very important for ergothioneine supply. The L. edodes cultivars Bambithyang (NIFoS 3404) and Sansanhyang (NIFoS 3420) of the National Institute of Forest Science (NIFoS) had higher ergothioneine content than the other cultivars, and 11 strains were obtained using mono-mono hybridization between them. The strains were inoculated into sawdust media to obtain fruiting bodies, and the ergothioneine content of each hybrid strain was confirmed using high-performance liquid chromatography (HPLC) analysis. Among the 11 strains, NIFoS 5108 had a higher ergothioneine content than the parental strains, Bambithyang (NIFoS 3404) and Sansanhyang (NIFoS 3420). This study revealed that it is possible to develop cultivars with improved specific functions using mono-mono hybridization.

• Applied Biological Chemistry
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• v.49 no.3
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• pp.186-191
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• 2006

In the present study, an attempt has been made to produce hybrid yeast strains of different useful and dominant characteristics. The hybrid yeast strains were produced by electrofusion and their genetic analysis were performed by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction). The protoplast of Saccharomyces cerevisiae KCTC 7904 and Zygosaccharomyces rouxii KCTC 7966 were obtained above 92% when treated with lyticase at $30^{\circ}C$ for $60{\sim}90$ min after the pretreatment of $1{\sim}2%$ 2-mercaptoethanol at $30^{\circ}C$ for $15{\sim}20$ min. The fusant was produced from paired protoplast stage under the electric pulse at high frequency conditions (1.5 MHz/50 pV, 615 $V/256\;{\mu}sec$) within glass-platinum made electrofusion chamber. Changes in RAPD patterns in mother cells and hybrid cells proved that the fusant contains two types of yeast gene originated from its parent. Furthermore, fermentation characters exhibits by the fusant cell confirmed its genetic changes. These results suggest that genetically stable hybrid yeast strains of economic importance can be produced by electrofusion technique and these electrofused yeast cells have an enormous impact in biotechnology and biomedicine.

• Korean Journal of Animal Reproduction
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• v.10 no.1
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• pp.27-35
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• 1986

As a preliminary experiment to establish the process on the sexing of mouse embryos by chromosomal analysis, present studies were carried out with inbred (ICR, C57BL) and F1 hybrid [(ICR${\times}$C57BL) = F1 ${\times}$ ICR] mice to investigate the blastomere numbers and mitotic indices (M.I.) to the developmental stage of embryos recovered, the optimum periods of anti-mitotic agent administration, the successful rates of sexing and sex-ratio. The results obtained were summarized as follows: 1. The blastomere numbers (mean${\pm}$S.E.) of the morula and blastocyst were 18${\pm}$0.4 and 54${\pm}$0.7, respectively. 2. Whereas the M.I. of F1 hybrid (16${\pm}$0.2%) was higher than that fo inbred ICR (15${\pm}$0.1%) and C57BL (12${\pm}$0.6%) in the different strains, the morula (7${\pm}$0.6%) was higher than that of blastocyst (6${\pm}$0.4%) in the case of embryo stages. 3. Following to anti-mitotic agents treated, the M.I. of embryos cultured with Colcemid (17${\pm}$1.1%) was superior to that fo embryos cultured with Velban (12${\pm}$0.9%) and the Colcemid injection (7${\pm}$0.4%). 4. The successful rate of sexing in the blastocyst (38.7%; 124/320) was superior to the morula (35.9%; 52/145), and the F1 hybrid (48.1%) was higher than that of inbred ICR (42.4%) and C57 BL (28.2%). 5. In the successful rate of sexing to the methods of administration, the embryos cultured with Colcemid (46.0%) was superior to that of embryos cultured with Velban (39.0%) and the Colcemid injection (38.8%). 6. Of 98 embryos sexed after culture with Colcemid, 89(90.8%) were observed between 2 and 4 hrs. In the case of Velban treatment, 83.1% (74/89) was observed between 2$\frac{1}{2}$ and 4$\frac{1}{2}$ hrs. 7. Out of 761 prepared embryos it was possible to sex 311; 157 were male and 154 were female, i.e.a sex-ratio of 50% a, pp.oximately.

• Structural Engineering and Mechanics
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• v.90 no.2
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• pp.159-175
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• 2024

In order to solve the problem of calculating the reasonable completed bridge state of a self-anchored hybrid cable-stayed suspension bridge (SA-HCSB), this paper proposes an analytical method. This method simplifies the main beam into a continuous beam with multi-point rigid supports and solves the support reaction forces. According to the segmented catenary theory, it simultaneously solves the horizontal forces of the main span main cables and the stay cables and iteratively calculates the equilibrium force system on the main beam in the collaborative system bridge state while completing the shape finding of the main span main cable and stay cables. Then, the horizontal forces of the side span main cables and stay cables are obtained based on the balance of horizontal forces on the bridge towers, and the shape finding of the side spans are completed according to the segmented catenary theory. Next, the difference between the support reaction forces of the continuous beam with multiple rigid supports obtained from the initial and final iterations is used to calculate the load of ballast on the side span main beam. Finally, the axial forces and strains of each segment of the main beam and bridge tower are obtained based on the loads applied by the main cable and stay cables on the main beam and bridge tower, thereby obtaining analytical data for the bridge in the reasonable completed state. In this paper, the rationality and effectiveness of this analytical method are verified through a case study of a SA-HCSB with a main span of 720m in finite element analysis. At the same time, it is also verified that the equilibrium force of the main beam under the reasonably completed bridge state can be obtained through iterative calculation. The analytical algorithm in this paper has clear physical significance, strong applicability, and high accuracy of calculation results, enriching the shape-finding method of this bridge type.

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.1
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• pp.101-105
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• 2004

A number of Chinese, Japanese and European silkworm strains with coarse size filament were used for breeding the coarse size variety. Through cross breeding combined with pedigree selection within thirteen generations, a pair of coarse size silkworm variety, named ${CU_1}{\times}{CU_2}$, was obtained after five years. Laboratory trials showed that the filament size of its 4F_1$ hybrid was over 4.3 D, and the other economic characters were also good.

• Proceedings of the Korea Concrete Institute Conference
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• 2001.11a
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• pp.509-514
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• 2001

The Fibre-optic Bragg grating (FBG) sensor is broadly accepted as a structural health monitoring device for Fibre reinforced plastic (FRP) materials by either embedding into or bonding onto the structures. The accuracy of the strain measured by using the FBG sensor is highly dependent on the bonding characteristics among the bare optical fibre, protective coating, adhesive layer and host material. In general, the signal extracted from the embedded FBG sensor should reflect the straining condition of the host structure. This paper presents a theoretical model to evaluate the differential strains between the bare fibre and host material with different adhesive thickness and modulus of the protective coating of the embedded FBG sensor.

• Proceedings of the Korea Concrete Institute Conference
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• 2005.11a
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• pp.53-56
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• 2005

This paper investigates the effect of ductile deformation behavior of high performance hybrid fiber-reinforced cement composites (HPHFRCCs) on the shear behavior of coupling beams to lateral load reversals. The matrix ductility and the reinforcement layout were the main variables of the tests. Three short coupling beams with two different reinforcement arrangements and matrixes were tested. They were subjected to cyclic loading by a suitable experimental setup. All specimens were characterized by a shear span-depth ratio of 1.0. The reinforcement layouts consisted of a classical scheme and diagonal scheme without confining ties. The effects of matrix ductility on deflections, strains, crack widths, crack patterns, failure modes, and ultimate shear load of coupling beams have been examined. The combination of a ductile cementitious matrix and steel reinforcement is found to result in improved energy dissipation capacity, simplification of reinforcement details, and damage-tolerant inelastic deformation behavior. Test results showed that the HPFRCC coupling beams behaved better than normal reinforced concrete control beams. These results were produced by HPHFRCC's tensile deformation capacity, damage tolerance and tensile strength.

• Journal of Aquaculture
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• v.12 no.2
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• pp.163-165
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• 1999

A production method for scaled and scaleless strain of common carp (Cyprinus carpio) at a breeding trial is developed by test-cross technique. When the fish with scaleless (Israeli strain of common carp) were crossed with scaled strain (hybrid fish between oriental and Israeli strain of the species), we can easily obtain 2 types of common carp with or without scale. The frequency of scaleless fish in this experiment was about 40% in the population, however, their growth rate was slightly higher than scaled fish after 3 months of this experiment.