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http://dx.doi.org/10.4489/KJM.20210049

Selection of Functional Lentinula edodes Strains with High Ergothioneine Content using Mono-mono Hybridization  

Kim, Min-Jun (Forest Microbiology Division, Forest Bioresources Department, National Institute of Forest Science)
Jeong, Yeun Sug (Forest Microbiology Division, Forest Bioresources Department, National Institute of Forest Science)
Jang, Yeongseon (Forest Microbiology Division, Forest Bioresources Department, National Institute of Forest Science)
Ka, Kang-Hyeon (Forest Microbiology Division, Forest Bioresources Department, National Institute of Forest Science)
Publication Information
The Korean Journal of Mycology / v.49, no.4, 2021 , pp. 507-514 More about this Journal
Abstract
Lentinula edodes have been cultivated extensively not only in South Korea but also in other Asian countries. In terms of taste and nutrition, it is a valuable mushroom that is comparable to other mushrooms. L. edodes contains various physiologically active compounds that promote human health. One compound, ergothioneine, exerts a powerful antioxidant function that inhibits cellular senescence. As it is not biosynthesized by plants or animals, L. edodes is very important for ergothioneine supply. The L. edodes cultivars Bambithyang (NIFoS 3404) and Sansanhyang (NIFoS 3420) of the National Institute of Forest Science (NIFoS) had higher ergothioneine content than the other cultivars, and 11 strains were obtained using mono-mono hybridization between them. The strains were inoculated into sawdust media to obtain fruiting bodies, and the ergothioneine content of each hybrid strain was confirmed using high-performance liquid chromatography (HPLC) analysis. Among the 11 strains, NIFoS 5108 had a higher ergothioneine content than the parental strains, Bambithyang (NIFoS 3404) and Sansanhyang (NIFoS 3420). This study revealed that it is possible to develop cultivars with improved specific functions using mono-mono hybridization.
Keywords
Ergothioneine; Lentinula edodes; Mono-mono hybridization;
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1 Shin PG, Oh MJ, Kim ES, Oh YL, Jang KY, Kong WS. Cultivation of 'ergo', a functional mushroom variety with enhanced ergothioneine content. In: 2016 Summer Conference; 2016 Jun 9-10; Jeonju-si, South Korea. The Korean Society of Mushroom Science; 2016. p. 190.
2 Liu SR, Ke BR, Zhang WR, Liu XR, Wu XP. Breeding of new Ganoderma lucidum strains simultaneously rich in polysaccharides and triterpenes by mating basidiospore-derived monokaryons of two commercial cultivars. Sci Hort 2017;216:58-65.   DOI
3 Kim JH, Kang YJ, Baek IS, Shin BE, Choi JI, Lee YS, Lee Y, Jeoung YK, Lee YS, Chi JH. Characteristics of Newly Bred Lentinula edodes Cultivar 'Hwadam' for Sawdust Cultivation. Kor J Mycol 2020;48:125-33.   DOI
4 Ha BS, Kim S, Ro HS. Isolation and characterization of monokaryotic strains of Lentinula edodes showing higher fruiting rate and better fruiting body production. Mycobiology 2015;43:24-30.   DOI
5 Muszynska B, Pazdur P, Lazur J, Sulkowska-Ziaja K. Lentinula edodes (Shiitake)-biological activity. Int J Med Rev 2017;27:189-95.
6 Hartman PE. Ergothioneine as antioxidant. Meth Enzymol 1990;186:310-8.   DOI
7 Melville DB, Eich S, Ludwig ML. The biosynthesis of ergothioneine. J Bio Chem 1957;224:871-7.   DOI
8 Melville DB, Eich S. The occurrence of ergothioneine in plant material. J Bio Chem 1956;218:647-51.   DOI
9 Kalaras MD, Richie JP, Calcagnotto A, Beelman RB. Mushrooms: a rich source of the antioxidants ergothioneine and glutathione. Food Chem 2017;233:429-33.   DOI
10 Jang Y, Park J, Ryoo R, Park Y, Ka KH. Ergothioneine contents of shiitake (Lentinula edodes) fruiting bodies on sawdust media with different nitrogen sources. Kor J Mycol 2016;44:100-2.   DOI
11 Kong WS, Yoo YB, Jhune CS, You CH, Cho YH, Park YH, Kim KH. Cultivation and characterization of commercial strain. J Mushroom 2007;5:1-6.
12 Yu YH, Pan HY, Guo LQ, Lin JF, Liao HL, Li HY. Successful biosynthesis of natural antioxidant ergothioneine in Saccharomyces cerevisiae required only two genes from Grifola frondosa. Microb Cell Factories 2020;19:1-10.   DOI
13 Ito T, Kato M, Tsuchida H, Harada E, Niwa T, Osawa T. Ergothioneine as an anti-oxidative/anti-inflammatory component in several edible mushrooms. Food Sci Technol Res 2011;17:103-10.   DOI
14 Mau JL, Lin HC, Song SF. Antioxidant properties of several specialty mushrooms. Food Res Int 2002;35:519-26.   DOI
15 Paul B, Snyder S. The unusual amino acid L-ergothioneine is a physiologic cytoprotectant. Cell Death Differ 2010;17:1134-40.   DOI
16 Cheah IK, Halliwell B. Ergothioneine; antioxidant potential, physiological function and role in disease. Biochim Biophys Acta Mol Basis Dis 2012;1822:784-93.   DOI
17 Park Y, Jang Y, Ryoo R, Lee B, Ka KH. Breeding and cultural characteristics of newly bred Lentinula edodes strain 'Sanjanghyang'. Kor J Mycol 2019;47:143-52.
18 Chen SY, Ho KJ, Hsieh YJ, Wang LT, Mau JL. Contents of lovastatin, γ-aminobutyric acid and ergothioneine in mushroom fruiting bodies and mycelia. LWT 2012;47:274-8.   DOI
19 Seebeck FP. In vitro reconstitution of mycobacterial ergothioneine biosynthesis. J Am Chem Soc 2010;132:6632-3.   DOI
20 Yoo YB, Kim IY, Kong WS, Jang KY, Oh SJ, Jhune CS. Strain improvement of Pleurotus ostreatus using self-fertility monospore isolate. J Mushroom 2006;4:48-52.
21 Kong WS, Yoo YB, Jhune CS, Jang WB, Choi JS, Kim KH. Characterization of a new brown commercial strain. J Mushroom 2008;6:115-20.
22 Ka KH, Ryoo R, Jang Y, Park Y, Jeong YS, Kang JJ, Heo G, Jeon SM. Characteristics of fruiting bodies formed upon monohybrid cross of Lentinula edodes strains. Kor J Mycol 2019;47:173-9.   DOI
23 Yang X, Lin S, Lin J, Wang Y, Lin JF, Guo LQ. The biosynthetic pathway of ergothioneine in culinary-medicinal winter mushroom, Flammulina velutipes (Agaricomycetes). Int J Med Mushrooms 2020;22:171-181.   DOI