• 미생물학회지
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• 제54권2호
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• pp.171-173
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• 2018

본 연구에서 이용된 Enterococcus faecium JB00008 (KACC 92186P) 균주는 전통발효식품인 청국장에서 분리되었다. 이 균주는 Escherichia coil에 대해 높은 항균활성능력을 보였다. 우리는 이 균주의 유전체의 염기 서열을 분석하였다. 유전체 초안은 500 bp 이상의 contig 34개로 조립되었으며, 크기가 2,847,295 bp이고, G + C 함량(%)는 37.84%이다. 유전체 초안에서 항균활성물질(bacteriocin) 중 하나인 enterocin과 연관된 유전자가 5개 확인되었다.

• 한국기계가공학회지
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• 제20권2호
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• pp.14-22
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• 2021

A plan for the development of reliability-based ROK amphibious assault vehicles is proposed. By analyzing the development case of the U.S. EFV, considerations for the successful development of the next-generation Korea Forces amphibious assault vehicle are presented. If the vehicle reliability can be improved to the level of the fourth highest priority electric unit for power units, suspensions, decelerators, and body groups, which have the highest priority among fault frequency items, a system level MTBF of 36.4%↑ can be achieved, and the operational availability can be increased by 3.5%↑. The next-generation amphibious assault vehicles must fulfill certain operating and performance requirements, the underlying systems must be built, and sequencing of the hybrid engine and the modular concept should be considered. Along with big-data- and machine-learning-based failure prediction, machine maintenance based on augmented reality/virtual reality and remote maintenance should be used to improve the ability to maintain combat readiness and reduce lifecycle costs.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2004년도 춘계 총회 및 학술대회
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• pp.17-28
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• 2004

현재 재배되고 있는 고려인삼은 혼계상태로서 개체간의 형질변이가 대단히 심하며, 종자채취를 4년 후에 하기 때문에 신품종육성을 위해서는 매우 오랜 기간이 필요하다. 그러나 식물형질전환기술의 발달로 목적하는 유전자를 식물체에 재도입하여 새로운 품종을 육성할 수 있는 기술이 보급되어 유용유전자만 있다면 단시간에 고기능성 신품종을 육성할 수 있을 것이다. 본 과제에서는 인삼의 유용유전자를 대량으로 발굴하기 위하여 인삼의 조직별, 종간 및 처리간에 의한 cDNA library 총 10종 이상을 제작하고 이들 cDNA library로부터 인삼의 유용유전자원을 확보하기 위하여 EST 20,000개를 5' 한쪽 방향에서 분석하고 이들 분석된 EST clone의 데이터는 data base화 하여 많은 연구자들이 연구정보를 공유한 수 있게 하였다. 그리고 EST clone 중에서 완전한 단백질의 유전정보를 포함하고 있는 clone을100개 선발하여 전장의 염기서열(full length sequence)을 분석하고 data base를 구축하고parental clone을 선발하여 cDNA chip을 제작하였다. 특히 257 clone 주에서 기능성 및 내재해성 유용유전자를 선발하여 전염기서열분석(full length sequencing)을 한 후 인상에 재도입하여 고기능성 및 내재해성 인삼의 분자육종을 비교적 단시간내에 할 수 있는 형질전환 및 재분화 시스템을 개발하고 토양순화 시스템을 확립하고자 하였다.

• Mycobiology
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• 제51권1호
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• pp.36-48
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• 2023

Xanthoria elegans is a lichen symbiosis, that inhabits extreme environments and can absorb UV-B. We reported the de novo sequencing and assembly of X. elegans genome. The whole genome was approximately 44.63 Mb, with a GC content of 40.69%. Genome assembly generated 207 scaffolds with an N50 length of 563,100 bp, N90 length of 122,672 bp. The genome comprised 9,581 genes, some encoded enzymes involved in the secondary metabolism such as terpene, polyketides. To further understand the UV-B absorbing and adaptability to extreme environments mechanisms of X. elegans, we searched the secondary metabolites genes and gene-cluster from the genome using genome-mining and bioinformatics analysis. The results revealed that 7 NR-PKSs, 12 HR-PKSs and 2 hybrid PKS-PKSs from X. elegans were isolated, they belong to Type I PKS (T1PKS) according to the domain architecture; phylogenetic analysis and BGCs comparison linked the putative products to two NR-PKSs and three HR-PKSs, the putative products of two NR-PKSs were emodin xanthrone (most likely parietin) and mycophelonic acid, the putative products of three HR-PKSs were soppilines, (+)-asperlin and macrolactone brefeldin A, respectively. 5 PKSs from X. elegans build a correlation between the SMs carbon skeleton and PKS genes based on the domain architecture, phylogenetic and BGC comparison. Although the function of 16 PKSs remains unclear, the findings emphasize that the genes from X. elegans represent an unexploited source of novel polyketide and utilization of lichen gene resources.

• Animal Bioscience
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• 제37권8호
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• pp.1345-1354
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• 2024

Objective: The objective of this study was to identify candidate genes that play important roles in skeletal muscle development in ducks. Methods: In this study, we investigated the transcriptional sequencing of embryonic pectoral muscles from two specialized lines: Liancheng white ducks (female) and Cherry valley ducks (male) hybrid Line A (LCA) and Line C (LCC) ducks. In addition, prediction of target genes for the differentially expressed mRNAs was conducted and the enriched gene ontology (GO) terms and Kyoto encyclopedia of genes and genomes signaling pathways were further analyzed. Finally, a protein-to-protein interaction network was analyzed by using the target genes to gain insights into their potential functional association. Results: A total of 1,428 differentially expressed genes (DEGs) with 762 being up-regulated genes and 666 being down-regulated genes in pectoral muscle of LCA and LCC ducks identified by RNA-seq (p<0.05). Meanwhile, 23 GO terms in the down-regulated genes and 75 GO terms in up-regulated genes were significantly enriched (p<0.05). Furthermore, the top 5 most enriched pathways were ECM-receptor interaction, fatty acid degradation, pyruvate degradation, PPAR signaling pathway, and glycolysis/gluconeogenesis. Finally, the candidate genes including integrin b3 (Itgb3), pyruvate kinase M1/2 (Pkm), insulin-like growth factor 1 (Igf1), glucose-6-phosphate isomerase (Gpi), GABA type A receptor-associated protein-like 1 (Gabarapl1), and thyroid hormone receptor beta (Thrb) showed the most expression difference, and then were selected to verification by quantitative real-time polymerase chain reaction (qRT-PCR). The result of qRT-PCR was consistent with that of transcriptome sequencing. Conclusion: This study provided information of molecular mechanisms underlying the developmental differences in skeletal muscles between specialized duck lines.

• Asian-Australasian Journal of Animal Sciences
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• 제20권5호
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• pp.627-632
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• 2007

FoxO1, FoxO3a and FoxO4 belong to the FoxO gene family, which play important roles in the PI3K/PKB pathway. In this study, we cloned the porcine FoxO1, FoxO3a and FoxO4 sequences and assigned them to SSC11p11-15, SSC1p13 and SSC xq13 using somatic cell hybrid panel (SCHP) and radiation hybrid panel (IMpRH). RT-PCR results showed that these three genes are expressed in multiple tissues. Sequencing of PCR products from different breeds identified a synonymous T/C polymorphism in exon 2 of FoxO3a. This FoxO3a single nucleotide polymorphism (SNP) can be detected by AvaII restriction enzyme. The allele frequencies of this SNP were investigated in Dahuabai, Meishan, Tongcheng, Yushan, Large White, and Duroc pigs. Association of the genotypes with growth and carcass traits showed that different genotypes of FoxO3a were associated with carcass length and backfat thickness between 6th and 7th ribs (BTR) and drip loss (p<0.05).

• 생명과학회지
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• 제30권6호
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• pp.522-531
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• 2020

본 연구는 반응표면분석법을 이용하여 L(+)형 젖산 생산향상을 위한 배지조성을 확립하기 위해 수행되었다. L(+)형 젖산을 선택적으로 고생산하는 것으로 알려진 9종의 Lactobacillus paracasei 균주를 전국에서 수집한 김치 시료로부터 선별하였으며, 젖산 생산량과 glucose로부터의 전환률 분석을 통하여 젖산 생산 배지 최적화를 수행하기 위한 균주로 SRCM201474를 선발하였다. 선택된 11개의 배지 조성 중 젖산 생산에 가장 큰 영향을 미치는 요인을 분석하기 위한 방법으로 Plack-Burman design (PBD)을 설계하였으며, 통계분석을 통해 탄소원으로는 glucose, sucrose, molasses, 질소원으로는 peptone을 최종 선정하였다. 젖산 생산 배지 최적화를 위한 각 변수들의 농도 최적화를 수행하기 위한 방법으로 반응표면분석법 중 적은 실험수로도 최적값을 산출할 수 있는 hybrid design 설계 하였다. 실험 모델에 의한 L. paracasei SRCM201474 균주를 이용한 젖산 생산배지 조성과 최적 농도는 glucose 15.48 g/l, sucrose 16.73 g/l, molasses 39.09 g/l, peptone 34.91 g/l로 나타났으며, 이때의 젖산 생산량은 33.38 g/l로 예측되었다. ANOVA 분석을 통해 가정된 실험 모델의 적합성과 유의성을 확인하였으며, 최종적으로 분석된 최적배지에서의 반복실험을 통한 젖산 생산량을 측정하여 모델에 의해 예측된 젖산생산량과 동일함을 검증하였다. 본 연구를 통해 L(+)형 젖산을 선택적으로 고생산하는 균주를 선발하였으며, 배지 최적화를 수행하여 생분해성 플라스틱 생산을 위한 산업적 젖산 생산에 적용할 수 있는 연구자료로 활용될 수 있을 것으로 판단된다.

• 한국어류학회지
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• 제29권2호
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• pp.139-145
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• 2017

최근 우리나라에서 자연발생적인 미꾸리속 알비노 개체들이 낮은 빈도로 출현하고 있으나, 색소 결핍으로 인해 형태적종 동정이 어렵다. 따라서 본 연구에서는 핵 유전자인 recombination activating gene 1 (rag1) 영역 및 미토콘드리아 유전자 cytochrome b (cytb) 영역을 이용한 분자계통학적 분석을 이용해 미꾸리속 알비노 개체의 분자동정을 수행하였다. 그 결과 rag1과 cytb의 분자계통도에서 미꾸라지, 미꾸리, 그리고 M. mohoity로 3개의 clade가 확인되었다. 확보된 M. mohoity의 염기서열을 유전자은행의 BLAST를 이용해 유사성을 검색한 결과 M. mohoity와 가장 유사하였다. 분자계통도를 기준으로 25마리의 알비노 미꾸리속 개체의 종 동정을 수행한 결과 빨간 눈 타입은 미꾸리 16마리, 미꾸라지 1마리로 판별되었고, 나머지 3개체는 미꾸라지♀${\times}$미꾸리♂ 잡종 1마리와 M. mohoity ♀${\times}$미꾸리♂ 잡종이 2마리 판별되었다. 또한 검은 눈타입 5마리는 미꾸리 1마리와 미꾸라지 3마리 및 M. mohoity 1마리로 판별되었다. 따라서 본 연구에 이용한 분자마커를 활용함으로써 미꾸리속 어류의 정확한 종 또는 잡종을 동정하기 위한 유용한 방법으로 이용될 수 있을 것이다.

• 미술이론과 현장
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• 제15호
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• pp.137-165
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• 2013

The prefix 'bio' with the meaning of 'life,' has been used for biotechnology, biochemistry, bioengineering, biomedicine, bioethics, bio-information as well as 'bio art' since 1990s. Bio art is an art as life itself and a kind of new direction in contemporary art that manipulates the processes of life. Bio artists use the properties of life and materials as scientists in laboratory of biology, and change organisms within their own species, of invents life with new characteristics. Technologically and socio-culturally, bio art has been connected with bioengineering. This essay is on the bio art that use vegetables, and on the specified gaze of so-called 'Sci-Artists.' Not only the genetically modified vegetables like works of George Gessert, Ackroyd & Harvey, and Eduardo Kac, but also the works made from the critical viewpoint like those of Paul Vanouse, Natalie Jeremijenko, and Amy Youngs, have 'the molecular gaze'(Suzanne Anker and Dorothy Nelkin's concept) of the genetic age in their art works. As the art history have showed, artists' gazes have insights about social problems that surround us. Bioartists' gazes reveal their insights about social and ethical problems, possibly concealed by science itself. Those problems are about results from practical discoveries of the sequencing of the genome, genetic engineering, cloning and reproduction of human and animals, body transformation, and the commercialization of cell and genes etc. We can find the significance of bioart in the molecular gaze about those problems, and we can rethink the identity of human, the reception of social influences from bio-technology and medicine.

• The Plant Pathology Journal
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• 제19권1호
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• pp.24-29
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• 2003

SS2-2, a hypernodulating soybean mutant was isolated by EMS mutagenesis from Sinpaldalkong 2. This auto-regulation mutant showed greater number of nodules and smaller plant size than its wild type Sinpaldalkong 2. SSR markers were used to identify DNA variation at SSR loci from different soybean LG. The only SSR marker that detected a length polymorphism between SS2-2 and its wild type ancestor was Satt294 on LG C1 instead of LG H, locating a hypernodulating gene. Sequencing data of flanking Satt294 indicated that the size variation was due to extra stretch of TTA repeats of the SSR motif in SS2-2, along with $A\longrightarrow$G transversion. In spite of phenotypic differences between the wild type and its hypernodulating mutants, genomic DNA poly-morphisms at microsatellite loci could not control regulation of nodule formation. The cDNA-AFLP method was applied to compare differential display of cDNA between Sinpaldalkong 2 and SS2-2. After isolation and sequence comparison with many AELP fragments, several interesting genes were identified. Northern blot analysis, immunolocalization and/or the yeast two-hybrid system with these genes might provide information on regulation of nodule development in SS2-2.