• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.233-238
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• 2005

This experiment was conducted from 2002 to 2004 at paddy field of National Livestock Research Institute, RDA, Suwon to investigate the select the promising double cropping system on growth, yield and nutritive value of summer forage(silage corn, sorghum$\times$ sudangrass hybrid, japanese millet, jobs tear, rice) and winter forage(rye, barley, Italian ryegass) and also to determine the best double cropping system necessary to maximize the total forage yield of winter forages plus summer forage crops. Among agronomic characteristics, lodging tolerance appeared to be highest in the summer forage crop including silage corn, jobs tear and rice compared to other forage crops. And lodging tolerance appeared to be highest in the winter forage crop including barley compared to rye and Italian ryegrass. The highest dry matter yield of 27,766 kg/ha, 27,296 kg/ha and 25,365 kg/ha obtained from an whole crop barley+sorghum$\times$ sudangrass hybrid, rye+sorghum$\times$ sudangrass hybrid and Italian ryegrass+sorghum$\times$sudangrass hybrid double cropping sequence in combination among the fifteen cropping systems((P<0.05). And dry matter yield of barley+silage corn and rye+silage com were 23,766 and 23,572 kg/ha.

• Journal of Life Science
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• v.25 no.8
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• pp.925-931
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• 2015

We performed a molecular marker-based analysis of quantitative trait loci (QTLs) for traits that determine the quality of the appearance of grains, using 120 doubled-haploid (DH) lines developed by another culture from the F1 cross between ‘Cheongcheong’ (Oryza sativa L. ssp. Indica) and ‘Nagdong’ (Oryza sativa L. ssp. Japonica). The traits studied included length, width, and thickness of the grains, as well as length-to-width ratio and 1,000-grain weight. The objective of this study was to determine the genetic control of these traits in order to formulate a strategy for improving the appearance of this hybrid. Within the DH population, five traits exhibited wide variation, with mean values occurring within the range of the two parents. Three QTLs were identified for grain length on chromosomes 2, 5, and 7. Three QTLs were mapped for grain width on chromosome 2: qGW2-1, qGW2-2, and qGW2-3. Six chromosomes were identified for the grain length-to-width ratio; four of these were on chromosome 2, whereas the other two were on chromosomes 7 and 12. One QTL influencing 1,000-grain weight was identified and located on chromosome 8. The results presented in the present study should facilitate rice-breeding, especially for improved hybrid-rice quality.

• Korean Journal of Plant Resources
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• v.30 no.5
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• pp.571-577
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• 2017

Abscisic acid (ABA) is an important phytohormone involved in abiotic stress tolerance in plants. The group A bZIP transcription factors play important roles in the ABA signaling pathway in Arabidopsis but little is known about their functions in rice. In our current study, we have isolated and characterized a group A bZIP transcription factor in rice, OsABF3 (Oryza sativa ABA responsive element binding factor 3). We examined the expression patterns of OsABF3 in various tissues and time course analysis after abiotic stress treatments such as drought, salinity, cold, oxidative stress, and ABA in rice. Subcellular localization analysis in maize protoplasts using a GFP fusion vector further indicated that OsABF3 is a nuclear protein. Moreover, in a yeast one-hybrid experiment, OsABF3 was shown to bind to ABA responsive elements (ABREs) and its N-terminal region found to be necessary to transactivate a downstream reporter. A homozygous T-DNA insertional mutant of OsABF3 is more sensitive to salinity, drought, and oxidative stress compared with wild type plants ＆ OsABF3OX plants. In addition, this Osabf3 mutant showed a significantly decreased sensitivity to high levels of ABA at germination and post-germination. Collectively, our present results indicate that OsABF3 functions as a transcriptional regulator that modulates the expression of abiotic stress-responsive genes through an ABA-dependent pathway.

• Korean Journal of Agricultural Science
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• v.28 no.1
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• pp.1-7
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• 2001

In this study we evaluated genetic divergence among six Tongil-type rice varieties and assessed the relationship between genetic distance and hybrid performance in all possible non-reciprocal crosses between them. The 15 F1 hybrids along with the six parents were evaluated for eight traits of agronomic importance including yield in a replicated field trial. The six parents were examined for DNA polymorphism using 120 random decamer oligonucleotide primers. A total of 168 polymorphic variants were generated and based on the polymorphism data, genetic distances (GDs) ranged from 0.157 to 0.383. Heterosis was observed in hybrids for most of the traits, and yield exhibited the highest heterosis among the eight traits examined. The correlation values of GDs with F1 performance were not significant. Also the correlations of GDs with midparent and better-parent heterosis were not significant except for spikelets per panicle. Our results have indicated that GDs based on the RAPDs markers may not be useful for predicting heterotic combinations in Tongil-type rice and are supportive of the idea that the level of correlations between hybrid performance and genetic divergence is dependent on the germlplasm employed.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.1
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• pp.46-50
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• 1984

Four rice cultivars (two japonicas and two japonica x indica hybrids) were grown in the hydroponic medium in order to investigate the photorespiration rate and ribulose-1, 5-bisphorphate carboxylase (EC 4, 1, 1;39) activity. The results were as follows: 1. Photorespiration rate was increased by the increasing light intensity as well as photosynthesis until light saturation point. Hangangchalbyeo of a hybrid variety had higher photorespiration rate than two japonicas(Jiniubyeo and Seolakbyeo) and one hybrid(Nampungbyeo) tested at ear formation stage. 2. Ribulose-1, 5-bisphosphate carborylase of hybrids were very sensitive to temperature showing lower activities at low temperature and much higher activities at high temperature than that of japonicas.

• BMB Reports
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• v.38 no.5
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• pp.602-608
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• 2005

As a crucial transcription factor family, heat-shock factors were mainly analyzed and characterized in tomato and Arabidopsis. In this study, we isolated two putative heat shock factors OsHSF6 and OsHSF12 that interact specifically with heat-shock element (HSE) from Oryza sativa L by yeast one-hybrid method. The full-length cDNA of OsHSF6 and OsHSF12 have 1074bp and 920bp open reading frame (ORF), respectively. Analysis of the deduced amino acid sequences revealed that OsHSF6 was a class A heat shock factor (HSF) with all the conserved sequence elements characteristic of heat stress transcription factor, while OsHSF12 was a class B HSF with C-terminal domain (CTD) lacking of AHA motif. Bioinformatic analysis showed that the sequences and structures of two HSFs' DNA binding domain (DBD) had a high similarity with LpHSF24. The results of RT-PCR indicated OsHSF6 gene was expressed immediately after rice plants exposure to heat stress, and the transcription of OsHSF6 gene accumulated primarily in immature seeds, roots and leaves. However, we did not find the transcription of OsHSF12 gene in different organs and growth periods. Our results implied that OsHSF6 might be function as a HSF regulating early expression of stress genes in response to heat shock, and OsHSF12 might be act as a synergistic factor to regulate the expression of down-stream genes.

• BMB Reports
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• v.42 no.1
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• pp.16-21
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• 2009

Three novel Class A genes that encode heat shock transcription factor (HSF) were cloned from Oryza Sativa L using a yeast hybrid method. The OsHSF7 gene was found to be rapidly expressed in high levels in response to temperature, which indicates that it may be involved in heat stress reception and response. Over-expression of OsHSF7 in transgenic Arabidopsis could not induced over the expression of most target heat stress-inducible genes of HSFs; however, the transcription of some HSF target genes was more abundant in transgenic plants following two hours of heat stress treatment. In addition, those transgenic plants also had a higher basal thermotolerance, but not acquired thermotolerance. Collectively, the results of this study indicate that OsHSF7 might play an important role in the response to high temperature. Specifically, these findings indicate that OsHSF7 may be useful in the production of transgenic monocots that can over-express protective genes such as HSPs in response to heat stress, which will enable such plants to tolerate high temperatures.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.3
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• pp.296-302
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• 1989

The mesocotyl elongation, having much effect on emergence and stand establishment in the direct sowing culture of rice, was investingated and summarized as follows: As to origin of cultivars, the average lengths of mesocotyl were 9.6 mm in the Indica-Japonica hybrid cultivars, 4.4 mm in the native cultivars and 3.2mm in the Japonica type, respectively. The mesocotyl lengths were tjereh, aman, aus, boro and bulu in order, with the mesocotyl lengths of 29.3mm and 5.4 mm in tjereh and bulu, respectively. The mesocotyl lengths were great in Baekkyungjo, Dadajo & Hejo among the native cultivars, in Sangpungbyeo, Paltal & Gokyangdo of the Japonica type and in Weonpungbyeo, Gayabyeo, Milyang 30 & Sujeongbyeo in Indica- Japonica hybrid cultivars, respectively. The mesocotyl length was the greatest at the seeds sampled I week after flowering (39. 3mm in length), and became decreased with the longer grain-filling. The mesocotyl elongated worse with the longer duration of seed storage regardless of seed maturity, and became longer at the 5$^{\circ}C$ storage plot than at the 15 and 25$^{\circ}C$ plots.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.2
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• pp.103-111
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• 1993

A greenhouse experiment was conducted to determine the number of optimum. tillers within a plant for high yield and to clarify the morpho-anatomical characteristics of cultivars having different tillering abilities. Optimum tillers, i.e tillers which produced heavy panicles were found to be around five to nine tillers per plant, although wider range may be possible if more cultivars were tested or under different growth conditions. Optimum tillers emerged within a short time after transplanting as compared with the other tillers. They exhibited longer tiller duration, produced more spikelets and had better filled spikelets, more vascular bundles and were taller with larger leaf area. However, the 1, 000 grain weight and fertility varied with cultivars and showed no general trend. Of this morpho-anatomical features, the total number of spike lets per plant was considered as the potential criterion for determining the optimum tiller number.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.4
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• pp.375-379
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• 2007

This study was carried out to examine the effects of storage duration on physico-chemical properties of milled rice. Rough rice of three rice ecotypes(Japonica, Tongil and F1 hybrids with Tongil back-ground) was stored for 4, 16, 28 and 40 months without artificial environmental control in seed warehouse. And this materials were investigated for seed germination rate, brown rice hardness, alkali digestion value(ADV), contents of protein, amylose, total sugar, and reducing sugar. Seed germination rate was significantly reduced with storage duration increased regardless of rice ecotypes : 66.7% from 16 months storage, 2.5% from 28, and nil from 40 months storage in Japonica rices. And 54.0%, 67.9% in Tongil, and F1 hybrid after 40 months storage, respectively. Brown rice hardness was not significantly affected by storage duration but showed significant genotypic differences. As the storage duration prolonged, sugar content and alkali digestion value increased, protein content declined while amylose content did not show clear tendency. Total and reducing sugar contents showed sharp increase upto 16 months storage and thereafter steady in crease, and the percentage of reducing sugar content out of total sugar content increased with extended storage duration.