Browse > Article
http://dx.doi.org/10.7732/kjpr.2017.30.5.571

Studies on OsABF3 Gene Isolation and ABA Signal Transduction in Rice Plants Against Abiotic Stress  

Ahn, Chul-Hyun (Department of General Education, Korea National College of Agriculture and Fisheries)
Park, Phun-Bum (Department of biochemical industry, The University of Suwon)
Publication Information
Korean Journal of Plant Resources / v.30, no.5, 2017 , pp. 571-577 More about this Journal
Abstract
Abscisic acid (ABA) is an important phytohormone involved in abiotic stress tolerance in plants. The group A bZIP transcription factors play important roles in the ABA signaling pathway in Arabidopsis but little is known about their functions in rice. In our current study, we have isolated and characterized a group A bZIP transcription factor in rice, OsABF3 (Oryza sativa ABA responsive element binding factor 3). We examined the expression patterns of OsABF3 in various tissues and time course analysis after abiotic stress treatments such as drought, salinity, cold, oxidative stress, and ABA in rice. Subcellular localization analysis in maize protoplasts using a GFP fusion vector further indicated that OsABF3 is a nuclear protein. Moreover, in a yeast one-hybrid experiment, OsABF3 was shown to bind to ABA responsive elements (ABREs) and its N-terminal region found to be necessary to transactivate a downstream reporter. A homozygous T-DNA insertional mutant of OsABF3 is more sensitive to salinity, drought, and oxidative stress compared with wild type plants ＆ OsABF3OX plants. In addition, this Osabf3 mutant showed a significantly decreased sensitivity to high levels of ABA at germination and post-germination. Collectively, our present results indicate that OsABF3 functions as a transcriptional regulator that modulates the expression of abiotic stress-responsive genes through an ABA-dependent pathway.
Keywords
ABA dependent; Abiotic stress; ABREs; bZIP; OsABF3;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Kim, B.R. and H.S. Kim. 2016. Effect on Wnt/${\beta}$-catenin pathway of methanol extracts from native plants in Korea. Korean J. Plant Res. 29(5):620-624.   DOI
2 Kwon, S.T., H.J. Jeong and P.M. Hasewgawa. 2010. Gene expression and response of arabidopsis AtSIZ3 mutants to temperature and drought stress. Korean J. Plant Res. 23(1):25-30.
3 Leung, J. and J. Giraudat. 1998. Abscisic acid signal transduction. Annu Rev Plant Physiol Plant Mol Biol. 49:199-222.   DOI
4 Lu, G., C. Gao, X. Zhong and B. Han. 2008. Identification of OsbZIP72 as a positive regulator of ABA response and drought tolerance in rice. Planta 229:605-15.
5 Miura, K., A. Rus, A. Sharkhuu, S. Yokoi, A.S. Karthikeyan, K.G. Raghothama, D. Baek, Y.D. Koo, J.B. Jin, R.A. Bressan, D.J. Yoon and P.M. Hasegawa. 2005. The Arabidopsis SUMO E3 ligase SIZ 1 controls phosphate deficiency response. Proc. Natl. Acad. Sci. 102:7760-7765.   DOI
6 Nakashima, K., Y. Fujita, K. Katsura, K. Maruyama, Y. Narusaka, M. Seki, K. Shinozaki and K. Yamaguchi-Shinozaki. 2006. Transcriptional regulation of ABI3 and ABA responsive genes including RD29B and RD29A in seeds, germinating embryos and seedlings of Arabidopsis. Plant Mol Biol. 60:51-68.   DOI
7 Oh, S.J., S.I. Song, Y.S. Kim, H.J. Jang, S.Y. Kim, M. Kim, Y.K. Kim, B.H. Nahm and J.K. Kim. 2005. Arabidopsis CBF3/DREB1A and ABF3 in transfenic rice increased tolerance to abiotic stress without stunting growth. Plant Physiol. 138:341-351.   DOI
8 Shinozaki, K. and K. Yamaguchi-Shinozaki. 2000. Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol. 3(3):217-23.   DOI
9 Singh, K., R.C., Foley and L. Onate-Sanchez. 2002. Transcription factors in plant defense and stress responses. Curr Opin Plant Biol. 5(5):430-6.   DOI
10 Uno, Y., T. Furihata, H. Abe, R. Yoshida, K. Shinozaki and K. Yamaguchi-Shinozaki. 2000. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc. Natl. Acad. Sci. 97:11632-11637.   DOI
11 Vernon, D.m. and H.J. Bohnert. 1992. Increased expression of a myo-lnositol methyl transferase in mesembryanthemum crystallinum Is part of a stress response distinct from crassulacean acid metabolism induction. Plant Physiol. 99:1695-1698.   DOI
12 Ciais, P., M. Reichstein and N. Viovy. 2005. Europe-wide reduction in primary productivity caused by the heat and drought in 2003. Nature 437:529-533.   DOI
13 Xiang, Y., N. Tang, H. Du, H. Ye and L. Xiong. 2008. Characterization of OsbZIP23 as a key player of basic leucine zipper transcription factor family for conferring Abscisic acid sensitivity and salinity and drought tolerance in rice. Plant Physiol. 148:1938-1952.   DOI
14 Yamaguchi-Shinozaki, K. and K. Shinozaki. 2005. Organization of cis-acting regulatory elements in osmotic and cold stress responsive promoters. Trends Plant Sci. 10:88-94.
15 Zhu, J.K. 2002. Salt and drought stress signal transduction in plants. Annu Rev Plant Biol. 53:247-273.   DOI
16 Bohnert, H.J. and R.G. Jensen. 1996. Strategies for engineering water-stress tolerance in plants. Trends Biotechnol. 14:89-97.   DOI
17 Chiera, j.m., J.G. Streeter and J.J. Finer. 2006. Ononitol and pinitol production in transgenic soybean containing the inositol methyl transferase gene from Mesembryanthemum crystallinum. Plant Sci. 171:647-654.   DOI
18 Himmelbach, A., U. Uang and E. Grill. 2003. Relay and control of abscisic acid signaling. Curr Opin Plant Biol. 6:470-479.   DOI
19 Finkelstein, R. R., S.S.L. Gampala and C.D. Rock. 2002. Abscisic acid signaling in seeds and seedlings. The Plant Cell 14:S15-S45.   DOI
20 Hasegawa, P.M., R.A. Bressan, J.K. Zhu and H.J. Bohnert. 2000. Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol, Plant Mol. Biol. 51:463-499.   DOI
21 Hurst, H.C. 1994. Transcription factors 1. bZIP proteins. Protein Profile 1:123-168.
22 Jakoby, M., B. Weisshaar, W. Droge-Laser, J. Vicente-Carbajosa, J. Riedemann, T. Kroj and F. Parcy. 2002. bZIP transcription factors in Arabidopsis. Trends Plant Sci. 7:106-111.   DOI
23 Abe, H., K.Y. Shinozaki, T. Urano, T. Iwasaki, D. Hosokawa and K. Shinozaki. 1997. Role of Arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression. Plant Cell 9:1859-58.
24 Kang, J.Y., H.I. Choi, M.Y. Im and S.Y. Kim. 2002. Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. The Plant Cell 14:343-357.   DOI
25 Kim, S.Y. 2007. Recent advances in ABA signaling. J Plant Biol. 50:117-121.   DOI