• Journal of Mushroom
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• v.16 no.3
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• pp.140-146
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• 2018

Pleurotus eryngii is one of the most commercially important mushrooms cultivated in Korea. However, the shelf-life of the fruiting body is short, limiting its export. A new hybrid strain H17 of P. eryngii was developed to extend the shelf-life by mono-mono crossing between monokaryotic strains derived from DanBi and KNR2774. Although the cultivation period of H17 was slightly longer than that of the reference cultivar Kenneutari No.2, the quality did not change and remained normal after a period of 65.0 days at $4^{\circ}C$. This result was significantly different from that of the reference cultivar Kenneutari No.2. Analysis of the genetic characteristics of the new hybrid strain H17 revealed a different profile from that of the parental and reference cultivars when random amplification of polymorphic DNA (RAPD) primers was used. These results demonstrate that H17 is a new cultivar with improved storability after harvesting.

• Journal of Mushroom
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• v.13 no.2
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• pp.129-134
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• 2015

Hybrid strains was selected by crossing between dikaryotic strain ASI 0628 (Dagul) bred from ASI 2596 (Suhan No.3) and ASI 2782(Black pileus mutant) and monokaryotic strain ASI 2344-84. The strain 84 that shown the best cultural characteristics was selected to be a new cytoplasmic hybrid variety and named as 'Chunhwashim'. The 'Chunhwashim' shown incompatibility among them forming the confrontation growth line against parental strains ASI 0628 (Dagul) and ASI 2344 (chunchu 2ho). Fruiting body produced about $161.4{\pm}4.8g$ per bottle. And also the individual generation of fruit body is multiple than chunchu2ho as 40.9. The 'Chunhwashim' was cytoplasmic hybrid included hybrid DNA bands of parental strains ASI 0628 (Dagul) and ASI 2344 (chunchu 2ho) by Random Amplified Polymorphic DNA (RAPD) primer, and mitochondria DNA band of monokaryotic ASI 2344 (chunchu 2ho) using mitochondria microsatellite DNA marker. This new cytoplasmic hybrid variety 'Chunhwashim' of oyster mushroom is characterized by multiple of individual generation and deeply grey color of pileus.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.3
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• pp.277-286
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• 1985

From a series of studies conducted on the local maize lines at the Agr. College of the Chungnam National Univ., a few maize lines with many tillers and ears per plant were identified and tentatively named as MET. The purpose of the study was to evaluate the MET lines, which were selfed for five generations, for silage purpose under the different plant densities. A hybrid, Suwon #19 and a synthetic variety, Puyo #3${\times}$#2, were included for comparison. Plant height at harvesting times showed no significant varietal differences. However, the MET lines were very slow in early plant growth compared to the hybrid or synthetic variety, probably due to inbreeding depression of the MET lines. Total fresh weight at the harvesting times was highest in the MET 1 line. The MET 1 line was about 2,000 kgr. per 10a. higher than the hybrid at the harvesting time. The highest fresh weight was obtained when grown under the plant density of 60 ${\times}$ 20cm. Total dry weight per unit area showed the same tendency as the fresh weight. Total dry weight of MET 1 line was about 2.4 tons per 10a., which was about 10% higher than the hybrid, Suwon #19. As the fresh weight, the total dry weight was also highest in the plant density of 60 ${\times}$ 20cm. The grain yield per 10 are of MET 1 was comparable to the grain yield of the hybrid, especially in the low plant density, 60 ${\times}$ 40cm. The average number of effective tillers of MET lines were 4.5, while the mean tiller number of the hybrid or synthetic variety were none. However, the lodging was one of the problem for growing MET lines. The 100 kernel weight of MET lines was about 9 gr., while the 100 kernel weight of the hybrid or synthetic variety was about 30 grm.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.2
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• pp.62-67
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• 1989

A breeding program in progress at Suweon Livestock Experiment Station, RDA, involves the hybridization of italian ryegrass (Lm) and several leading forage grasses in an effort to combine the nutritive, productivity and palatability qualities of Lm with the adaptive and cold resistance qual; .les of several leading grasses. In order to study the fate of the hybridization between remotely related species, immatured hybrid embryos were cultured on media. The emasculated plants of Lm were Sikem and Tetrone. Reweille, 2n=14, of perennial ryegrass (Lp.), Forager, 2n=42, of tall fescue(Fa), First, 2n=14,of meadow fescue(Fp), Potomac, 2n=28, of orchardgrass(Dg), and Richmond, 2n=42, of timothy(Ph.p) were used as pollinators. Embryos were isolated on 4, 8, 12, 16, and 20 days after pollination and cultured them on modified Murashige and Skoog media. Calluses and plantlets have been obtained after 8 days old embryos crossing between $Lm{\times}Fa$, after 12 days embryos crossing between $Lm{\times}Lp$, Dg, and Ph.p, and after 16 days embryos crossing between $Lm{\times}Fp$. Both callus and shoot formation occurred on 6 % or less of the plated embryos of $Lm{\times}Fp$, Dg, and Ph.p. Embryoderived callus forming shoots have been obtained from 4 days old embryo crossing between $Lm{\times}Dg$ which has not successfully been done anywhere as far as we know. It means that hybrid plants of species without crossability can be obtained through the use of immatured embryo culture. Some of plated embryos developed directly shoots from embryos and the others shoot-forming callus. Cross between related species showed a high frequency of directly shoot formation from plated embryos and cross between remotely related species a high frequency of callus formation.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.2
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• pp.147-151
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• 2005

Serious attempt for the improvement of quality parameters in silkworm hybrids came rather very late in India. Realising the need for productive breeds to the field and reorientation in breeding methodology, the silkworm breeders of CSRTI, Mysore were able to develop silkworm breeds with fine denier namely, JPN7, CSR48, B63 and B65 of specific industrial requirement through directional selection. As the success of silkworm breeds mainly depends on their combining ability, the developed breeds were subjected for hybrid evaluation. Out of 42 bivoltine hybrids studied, one hybrid, $CSR48{\times}CSR5$ was identified for its superiority over the existing bivoltine hybrids namely $CSR2{\times}CSR4,\;CSR2{\times}CSR5\;and\;CSR18{\times}CSR19$ for majority of qualitative traits. Post cocoon testing of large quantity of cocoons of $CSR48{\times}CSR5$ resulted in the production of high quality '3A'grade silk. It is for the first time in the Indian Sericulture industry that high quality silk with longer filament length of 1474 m, denier of 2.47, standard size deviation of 0.983 d, maximum size deviation of 1.322, neatness of 96 p, reelability of $85\%$, tenacity of 3.87 g/d, elongation of $22.32\%$ and cohesion of 110 strokes was produced. The superiority of fine denier silkworm breeds and their hybrids are discussed.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.162-162
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• 2017

Atractylodes japonica and Atractylodes macrocephala belongs to Atractylodes genus and theirs roots are used as 'Baek-chul'(White atractylodes rhizome) in Korea Pharmacopoeia. 'Baek-chul' widely used in traditional herbal remedies in Asia. Concurrently, the 'Baek-chul' demand enormously increased in the market due to the growing interest in favoring health benefits but it is depend on import from China. Thus we conducted breeding research to solve the problems associated with domestically cultivated 'Baek-chul' such as low productivity and low disease tolence. We crossed A. japonica (female parent) and A. macrocephala (male parent) in the greenhouse in September 2000. the next season 119 crossed line seeds were collected and germinated in the green house. Among them we selected the good lines in the same season. then conducted replicated yield trials (RYT) in 2014 to local adaptability test (LAT) in 2015-2016. The results showed that the 'AJM16' line has beneficial charactersitcs like, increased plant height, large rhizome diameter, narrow leaf width compared with a 'Sang-chul' (check cultivar) and varied flower color from A. japonica (female parent). The line 'AJM16' was also exhibited decreased anthracnose symptoms(Colletotrichum gloeosporides Penz.). Simultaneously the root yield of AJM16 was 2,409 kg per are, which was increased 130% higher yield compared to 'Sang-chul' (check cultivar) at three various regions from 2015 to 2016. Therefore, we named AJM16 as new cultivar 'Man-chul'. According the results, we have developed a new Atractylodes spp. hybrid cultivar 'Macn-chul' with high yielding and disease tolerance by National Institute of Horticulture and Herbal Science, RDA, in 2016.

• Journal of the Korean Society of Tobacco Science
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• v.7 no.2
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• pp.99-109
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• 1985

The present studies were carried out to obtain some basic informations of the breeding of tobacco varieties. Genetically divergent 8 varieties, 3 flue-cured, 2 burley and 3 sun-cured tobaccos, were used in half diallel cross. In order to analyze the heterosis, combining abilities, modes of inheritance and correlations for some agronomic and chemical characters, 8 parents and 28 $F_1$ were tested. The results obtained were summarized as follows: The percentages of heterosis for stem diameter, internode length and total sugar content in $F_1$ hybrid were 3.6%, 3.1% and 10.6%, whereas these for days to flower, total alkaloids and leaves per plant were -6.3%, -6.9% and -5.0%, respectively. Yield had significant positive genotypic correlations with plant height, days to flower and leaf length, but negative with internode length and total sugar content. It also had significant prositive phenotypic correlations with plant height, days to flower, leaves per plant, leaf length, leaf width and leaf shape index (Leaf length/leaf width). General (GCA) and specific combining abilities (SCA) for all characters of $F_1$ hybrid were significant. The effects of GCA were positive on yield, plant height, stem diameter, leaves per plant and days to flower of Burley 21. And those were positive on yield, leaf shape index and plant height, but negative on leaves per plant and total nitrogen of Hicks. The effects of SCA for yield and leaves per plant were greater than those of others on the combinations of Coker 139 and Burley type, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.9
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• pp.1319-1326
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• 2007

Fructose-1,6-bisphosphatase (FBP1) is a key regulatory enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose-1,6-bisphosphate to generate fructose-6-phosphate and inorganic phosphate. Deficiency of fructose-1, 6-bisphosphatase is associated with fasting hypoglycemia and metabolic acidosis. The enzyme has been shown to occur in bacteria, fungi, plants and animals. The bovine FBP1 gene was cloned and characterized in this study. The full length (1,241 bp) FBP1 mRNA contained an open reading frame (ORF) encoding a protein of 338 amino acids, a 63 bp 5' untranslated region (UTR) and a 131 bp 3' UTR. The bovine FBP1 gene was 89%, 85%, 82%, 82% and 74% identical to the orthologs of pig, human, mouse, rat and zebra fish at mRNA level, and 97%, 96%, 94%, 93% and 91% identical at the protein level, respectively. This gene was broadly expressed in cattle with the highest level in testis, and the lowest level in heart. An intronic single nucleotide polymorphism (SNP) (A/G) was identified in the $5^{th}$ intron of the bovine FBP1 gene. Genotyping of 133 animals from four beef breeds revealed that the average frequency for allele A (A-base) was 0.7897 (0.7069-0.9107), while 0.2103 (0.0893-0.2931) for allele B (G-base). Our preliminary association study indicated that this SNP is significantly associated with traits of Average Daily Feed Intake (ADFI) and Carcass Length (CL) (p<0.01). In addition, the FBP1 gene was assigned on BTA8 by a hybrid radiation (RH) mapping method.

• Journal of Sericultural and Entomological Science
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• v.51 no.1
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• pp.63-67
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• 2013

A new silkworm variety "WhangBoJam" for spring rearing season is F1 hybrid between Jam317, a japanese strain bred from introduction breeding and Jam318, a chinese strain from introduction breeding. In the local adaptability test performed at 8 local areas in spring of 2012, the hatchability rate of WhangBoJam was recorded 95% similar to Kum HwangJam. The larval period was 8hours more long than KumHwangJam. The pupation percentage was recorded 6.6% higher than KumHwangJam. Single cocoon weight was recorded 2.29 g similar to KumHwangJam and cocoon yield(21.3 kg) was higher than KumHwangJam. The concentration of DNJ was measured to 0.22% in WhangBoJam, lower than DaePoongJam(0.26%). In the Paecilomyces tenuipes production ability test, the pupal weight of Whang BoJam was 1.33 g/individual, higher than KumHwangJam (1.19 g/individual).

• Journal of Sericultural and Entomological Science
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• v.48 no.2
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• pp.37-40
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• 2006

A new silkworm variety "Daepoongjam" for spring rearing season is $F_1$ hybrid between Jam151, a Japanese race bred from introduction breeding and Jam152, a Chinese race from introduction breeding. In the local adaptatability test performed at 8local areas in spring of 2005, the heavy cocoon yield of "Daepoongjam" was recorded 6% higher than "Kumokjam". The concentration of Deokynojirimycin (DNJ) was measured 4.22 mg in "Daepoongjam", similar to "Kumokjam" (4.26 mg). In the Peacilomyces tenuipes production ability test, the pupal weight of "Daepoongjam" was 1.61 g/individual 13% heavier than "Kumokjam" (1.41 g/individual)