• The Journal of Advanced Prosthodontics
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• v.9 no.6
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• pp.447-452
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• 2017

PURPOSE. To evaluate the sealing performance of Hybrid Coat and its influence on the shear bond strength of five dentin surface cements. MATERIALS AND METHODS. Six premolars were pretreated to expose the dentin surface prior to the application of Hybrid Coat. The microscopic characteristics of the dentinal surfaces were examined with scanning electron microscopy (SEM). Then, 40 premolars were sectioned longitudinally, and 80 semi-sections were divided into a control group (untreated) and a study group (treated by Hybrid Coat). Alloy restoration was bonded to the teeth specimen using five different cements. Shear bond strength was measured by the universal testing machine. The fracture patterns and the adhesive interface were observed using a stereomicroscope. RESULTS. SEM revealed that the lumens of dentinal tubules were completely occluded by Hybrid Coat. The Hybrid Coat significantly improved the shear bond strength of resin-modified glass ionomer cement (RMGIC) and resin cement (RC) but weakened the performance of zinc phosphate cement (ZPC), zinc polycarboxylate cement (ZPCC) and glass ionomer cement (GIC). CONCLUSION. Hybrid Coat is an effective dentinal tubule sealant, and therefore its combined use with resin or resin-modified glass ionomer cements can be applied for the prostheses attachment purpose.

• Elastomers and Composites
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• v.54 no.3
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• pp.247-251
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• 2019

Two different urethane acrylates (mono-acrylate and di-acrylate) were used to prepare unsaturated polyester-based hybrid gel-coats. The physical properties and surface characteristics of these gel-coats were investigated on the basis of the content and type of urethane acrylate. The set-to-touch time increased and the physical properties (surface hardness and tensile strength) decreased with an increase in the urethane acrylate content. However, the type of urethane acrylate did not affect these parameters. It was found that the optimal urethane acrylate content for the application of unsaturated polyester-based hybrid gel-coats is ~10 wt%.

• Journal of the Korean Applied Science and Technology
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• v.28 no.3
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• pp.359-366
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• 2011

In this study we experimented that how polyurethane effect to acrylic-polyurethane resin in Full-Grain leather coatings. First of all, we consummated waterborne acrylic emulsion and waterborne polyurethane resin, Than we prepared F.G leathers which were coated by acrylic resin and acrylic-polyurethane resins. According to measured data for solvent resistance, acrylic resin and acrylic-polyurethane resins had good property. Sample a(WAC) had most low strength($2.10kg_f/mm^2$) and sample d(WAC 93 : WPU 7) had most high strength($3.41kg_f/mm^2$). Also we knew that most good property of abrasion is d(47.4 mg). In elongation case, a(WAC) had most good result(645 %) in this experiment.

• Restorative Dentistry and Endodontics
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• v.25 no.2
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• pp.180-192
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• 2000

One bottle system was recently developed in order to simplify the clinical skills and save chair time after continuous improvements on dentin bonding agents. There has been many studies to measure the bond strength of one bottle systems but no actual work has been done on micromorphologic study of resin-dentin interdiffusion zone after one bottle system application. To evaluate the bonding patterns of various commercially available one bottle systems to dentin, observation of resin-dentin interdiffusion zone under TEM was performed. Caries-free human third molars within one month of extractions were chosen for the experiments. The molars were sectioned 1mm above the cementoenamel junction and got rid of the root portions. Crown portions of the teeth were sectioned parallel to occlusal surface so that dentin discs of 1mm in thickness were remained. 7 one bottle systems and 1 two bottle system were applied according to manufacturer's instructions and followings were the results. 1. In every experimental groups, cross bandings of collagen fiber were distinguishable and tight bon dings between the bonding agents and dentin were observed. 2. Hybrid layer was clearly observed in ONE-STEP$^{(R)}$, Prime & Bond$^{(R)}$ 2.1, Syntac$^{(R)}$ SC, MAC-BOND II groups but it was not clear in Single Bond, D-Liner Dual PLUS, ONE COAT BOND groups. 3. Electron-density of hybrid layer was uniform in pattern in MAC-BOND II, Prime & Bond$^{(R)}$ 2.1 groups but not so uniform in ONE-STEP$^{(R)}$ group. 4. Electron-dense amorphous phase in most superior layer of the resin-dentin interdiffusion zone was characteristically observed in Single Bond, Syntac$^{(R)}$ SC, ONE COAT BOND groups. It can be concluded that bondings between the dentin bonding agents and dentin can be various in pattern according to their chemical compositions and the condition during applications.

• Molecules and Cells
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• v.19 no.3
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• pp.418-427
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• 2005

When inoculated into sensitive tobacco Xanthi-nn plants, the crucifer and garlic-infecting Tobacco mosaic virus (TMV-Cg) induces local necrotic lesions that resemble those seen in the hypersensitive response (HR) of resistant tobacco plants. However, unlike these, tobacco Xanthi-nn plants do not become resistant to infection and the virus spreads systemically causing a severe disease characterized by necrotic lesions throughout the plant. To identify the viral protein that elicits this necrotic response, we used a set of hybrid viruses constructed by combination of TMV-Cg and the tobacco mosaic virus strain U1 (TMV-U1). In this study we present evidence that the coat protein of TMV-Cg (CPCg) is the elicitor of the necrotic response in tobacco Xanthi-nn plants. Local and systemic necrotic lesions induced by TMV-Cg and by the hybrid U1-CPCg -that carries CPCg in a TMV-U1 context- are characterized by cell death and by the presence of autoflorescent phenolic compounds and $H_2O_2$, just like the HR lesions. In addition, defense-related genes and detoxifying genes are induced in tobacco Xanthi-nn plants after TMV-Cg and U1-CPCg inoculation. We postulate that in our system, CPCg is recognized by sensitive tobacco plants that mount an incomplete defense response. We call this an HR-like since it is not enough to induce plant resistance.

• Korean Journal of Veterinary Service
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• v.30 no.4
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• pp.557-562
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• 2007

This study was carried out to discriminate Hanwoo from the milking and hybrid cattle by detection of MC1R gene related to bovine hair color. One hundred sixty six samples were collected from the abattoir (n = 106) and local market (n = 60). The beef from abattoir were originated from Hanwoo (n=27), Holstein (n=29), Hybrid (n=45) and imported cattle (n=5), respectively. The beef from market consisted of Hanwoo (n=36), Holstein (n=7) and imported ones (n=17). Commercialized screening kit (Kogenebiotec, Korea) was used for MC1R gene analysis. As a result, Hanwoo was discriminated from Holstein. However, 9 of 45 hybrid and 11 of 22 imported beef samples were indistinguishable from Hanwoo. It could be explained by second generation of crossing of Hanwoo with Holstein or the cattle with silver or yellow hair. This results suggest that additional tests as well as MC1R gene detection be needed to confirm Hanwoo beef among cattle beef.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.4
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• pp.247-252
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• 2002

“DK 537”has been selected by forage crop breeding team at the National Livestock Research Institute(NLRI), RDA and has been determined by the Deliberative Council of the National Agricultural Cooperative Federation(NACF) in 2002, as a new recommended hybrid which is early maturing, good quality and hi호 yield of corn for silage. The characteristics of this hybrid are as follows ; 1. The seed coat is yellow and the mean of tasseling date is 5th of July, included in early maturing hybrid which is one day delayed than check cultivar, DK 501. The culm length is 246cm. It is resistant to lodging because of its low height from surface to ear. 2. DK 537 shows resistance to H. maydis and Maize Black-Streaked Dwarf Virus(MBSDV). It also shows strength to corn borer as much as DK 501 does. 3. Fresh yield. dry matter yield. and TDN per a ha are 50 tons, 16.9 tons, and 11.4 tons respectively. which are almost same yielding level of DK 501. Its percent ear to total dry matter is 50.8% at the same time. Through all these tests, we could make sure at DK 537 hybrid will be recommended as good forage crop.

• Korean Chemical Engineering Research
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• v.49 no.3
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• pp.314-319
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• 2011

In this study, the flow behavior of Newtonian and non-Newtonian coating liquids inside slot die has been scrutinized for the purpose of optimal internal die design in slot coating system from three-dimensional computations by CFD Fluent solver. A hybrid slot die could be optimally designed by changing the chamber or manifold structure to guarantee the uniform velocity distribution of coating liquids at die exit. Especially, for the non-Newtonian coating liquids, the length of coat-hanger for the uniform coating has been properly chosen, according to the degree of their shearthinning properties.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.4
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• pp.253-258
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• 2002

NC+4880, a forage com hybrid, is bred by NC+hybrids Co. in US, and Gast 8396 IT is by Garst Seeds Co. We have tested these two cultivars in Suwon and Chonan over three years to test their regional yield trial. Through above experiments, the excellence is testified, in result, they are determined as new recommended cultivars by NACF. The main characteristics of these cultivars are as tallows ; 1. NC+4880 a. The seed coat is yellow, the mean tasseling date is 7th of July and it takes about 74 days from emergence to tasseling date. This hybrid is a day ahead and the culm length is 242 cm, moreless shorter comparing with a check hybrid, Suwon 19. The ear height 117 cm which is short and its stay green is moderate. b. It shows resistance to H maydis, and MBSDV is barely observed and its stay green is moderate. c. The fresh yield, dry matter yield, and TDN yield per a ha are 55.4 tons, 18.3 tons, 12.4 tons, respectively, which are increased by 6%, 7%, and 9%, respectively, comparing with a check hybrid, Suwon 19. The percent ear among total dry matter yield is 50.9%. 2. Garst 8396 IT a. The seed coat is yellow, the mean tasseling date is 9th of July and it takes about 76 days from emergence to tasseling date. This hybrid represent a day delayed compared with a check hybrid, Suwon 19. The Culm length and the ear height are 275 cm, and 138 cm, respectively. b. It resists moderately to H. maydis, and highly resists to lodging in the field conditions. MBSDV is barely observed and its stay green is moderate. c. The fresh yield per a ha is 64.9 tons, dry matter yield, 20.9 tons, and TDN, 13.8 tons, respectively, which are increased by 25%, 23%, and 22% comparing with a check hybrid, Suwon 19. The percent ear among total dry matters is 43.3%.

• The Plant Pathology Journal
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• v.23 no.4
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• pp.281-286
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• 2007

Interactions between viral proteins and host proteins are essential for virus replication. Especially, translation of viral genes completely depends on the host machinery. In potyviruses, interactions of genome-linked viral protein (VPg) with host translation factors including eIF4E, eIF(iso)4E, and poly(A)-binding protein (PABP) has previously been characterized. In this study, we investigated interactions between Soybean mosaic virus (SMV) viral proteins and host translation factors by yeast two-hybrid system. SMV VPg interacted with eIF4E, eIF(iso)4E, and PABP in yeast two-hybrid system, while SMV helper component proteinase (HC-pro) interacted with neither of those proteins. The interaction between SMV NIb and PABP was also detected. These results are consistent with those reported previously in other potyviruses. Interestingly, we found reproducible and specific interactions between SMV coat protein (CP) and PABP. Deletion analysis showed that the region of CP comprising amino acids 116 to 206 and the region of PABP comprising amino acids 520 to 580 are involved in CP/PABP interactions. Soybean library screening with SMV NIb by yeast two-hybrid assay also identified several soybean proteins including chlorophyll a/b binding preprotein, photo-system I-N subunit, ribulose 1,5-biphosphate carboxylase, ST-LSI protein, translation initiation factor 1, TIR-NBS type R protein, RNA binding protein, ubiquitin, and LRR protein kinase. Altogether, these results suggest that potyviral replicase may comprise a multi-protein complex with PABP, CP, and other host factors.