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Tobamovirus Coat Protein CPCg Induces an HR-like Response in Sensitive Tobacco Plants  

Ehrenfeld, Nicole (Departamento de Genetica Moleculary Microbiologia, P. Universidad Catolica de Chile)
Canon, Paola (Departamento de Genetica Moleculary Microbiologia, P. Universidad Catolica de Chile)
Stange, Claudia (Departamento de Genetica Moleculary Microbiologia, P. Universidad Catolica de Chile)
Medina, Consuelo (Departamento de Genetica Moleculary Microbiologia, P. Universidad Catolica de Chile)
Arce-Johnson, Patricio (Departamento de Genetica Moleculary Microbiologia, P. Universidad Catolica de Chile)
Publication Information
Molecules and Cells / v.19, no.3, 2005 , pp. 418-427 More about this Journal
Abstract
When inoculated into sensitive tobacco Xanthi-nn plants, the crucifer and garlic-infecting Tobacco mosaic virus (TMV-Cg) induces local necrotic lesions that resemble those seen in the hypersensitive response (HR) of resistant tobacco plants. However, unlike these, tobacco Xanthi-nn plants do not become resistant to infection and the virus spreads systemically causing a severe disease characterized by necrotic lesions throughout the plant. To identify the viral protein that elicits this necrotic response, we used a set of hybrid viruses constructed by combination of TMV-Cg and the tobacco mosaic virus strain U1 (TMV-U1). In this study we present evidence that the coat protein of TMV-Cg (CPCg) is the elicitor of the necrotic response in tobacco Xanthi-nn plants. Local and systemic necrotic lesions induced by TMV-Cg and by the hybrid U1-CPCg -that carries CPCg in a TMV-U1 context- are characterized by cell death and by the presence of autoflorescent phenolic compounds and $H_2O_2$, just like the HR lesions. In addition, defense-related genes and detoxifying genes are induced in tobacco Xanthi-nn plants after TMV-Cg and U1-CPCg inoculation. We postulate that in our system, CPCg is recognized by sensitive tobacco plants that mount an incomplete defense response. We call this an HR-like since it is not enough to induce plant resistance.
Keywords
Systemic Hypersensitive Reaction;
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