Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Tobamovirus Coat Protein CPCg Induces an HR-like Response in Sensitive Tobacco Plants

  • Ehrenfeld, Nicole (Departamento de Genetica Moleculary Microbiologia, P. Universidad Catolica de Chile) ;
  • Canon, Paola (Departamento de Genetica Moleculary Microbiologia, P. Universidad Catolica de Chile) ;
  • Stange, Claudia (Departamento de Genetica Moleculary Microbiologia, P. Universidad Catolica de Chile) ;
  • Medina, Consuelo (Departamento de Genetica Moleculary Microbiologia, P. Universidad Catolica de Chile) ;
  • Arce-Johnson, Patricio (Departamento de Genetica Moleculary Microbiologia, P. Universidad Catolica de Chile)
  • Received : 2005.02.03
  • Accepted : 2005.03.11
  • Published : 2005.06.30
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Abstract

When inoculated into sensitive tobacco Xanthi-nn plants, the crucifer and garlic-infecting Tobacco mosaic virus (TMV-Cg) induces local necrotic lesions that resemble those seen in the hypersensitive response (HR) of resistant tobacco plants. However, unlike these, tobacco Xanthi-nn plants do not become resistant to infection and the virus spreads systemically causing a severe disease characterized by necrotic lesions throughout the plant. To identify the viral protein that elicits this necrotic response, we used a set of hybrid viruses constructed by combination of TMV-Cg and the tobacco mosaic virus strain U1 (TMV-U1). In this study we present evidence that the coat protein of TMV-Cg (CPCg) is the elicitor of the necrotic response in tobacco Xanthi-nn plants. Local and systemic necrotic lesions induced by TMV-Cg and by the hybrid U1-CPCg -that carries CPCg in a TMV-U1 context- are characterized by cell death and by the presence of autoflorescent phenolic compounds and $H_2O_2$, just like the HR lesions. In addition, defense-related genes and detoxifying genes are induced in tobacco Xanthi-nn plants after TMV-Cg and U1-CPCg inoculation. We postulate that in our system, CPCg is recognized by sensitive tobacco plants that mount an incomplete defense response. We call this an HR-like since it is not enough to induce plant resistance.

Keywords

Acknowledgement

Supported by : FONDECYT

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