• Title/Summary/Keyword: Human skin model

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Evaluation of Senescence Induced Prematurely by Stress. Application for cosmetic active ingredients

  • Morvan, Pierre-Yves;Romuald Vallee
    • 대한화장품학회:학술대회논문집
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    • 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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    • pp.285-290
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    • 2003
  • Living cells are continuously subject to all sorts of stress such as ultraviolet rays on skin cells. Tests made in various laboratories show that when young fibroblasts (Le. at the beginning of their proliferate life) were repeatedly put under stress at subletal doses, they acquired a phenotype similar to Senescence Induced Prematurely by Stress (SIPS). The work presented hereafter was made on a new model of senescence induced prematurely by stress from ultraviolet Brays (UVB). The human fibroblast model was put under repeated UVB stress, causing SIPS. Several ageing biomarkers were used in order to characterise the cells that underwent stress:. an increase in the proportion of positive cells with senescence associated $\beta$-galactosidase activity (SA $\beta$-gal) measured by a specific coloration,. the proportion in the different morphological stages that fibroblasts undergo during culture visualised by microscopic observation,. the expression of genes known for overexpressing during senescence, particularly fibronectin and apolipoprotein J, measured by Real Time-PCR,. the common deletion of 4,977 bp in mitochondrial DNA, evaluated by nested PCR. Studying the variation of these 4 biomarkers, we have evaluated the protective effect of a Laminaria digitata extract (LDE) that can be used as a natural active ingredient for anti-ageing cosmetics.

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단백질 오염의 세척거동에 관한 연구(I) -세척 시험용 모델 오염으로서의 인체 표피 각질층의 특성- (Studies on the Removal of Protein Soils ( I ) -Characterization of Human Epidermal Stratum Corneum as Model Soils for Detergency Test-)

  • 이정숙;김성련
    • 한국의류학회지
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    • 제10권3호
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    • pp.1-8
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    • 1986
  • The purpose of this study was to investigate the characteristics of human epidermal stratum corneum as protein model soils for detergency test. The stratum corneum was collected by scraping of the skin and purified with solvent. The results obtained were as follows: 1. Purified stratum corneum contained $92.38\%$ of crude protein. 2. In the amino acid compositions, contents of glycine, glutamic acid and serine were high and methionine and cystine were low. They were similar to fibrous $\alpha$-keratin consisted of stratum corneum. Whereas the content of polar amino acids was decreased, that of nonpolar amino acids was increased after enzyme hydrolysis. 3. The hydrolysis of stratum corneum with enzyme increased muck at initial reaction time and levelled off in 4$\~$6 hours. The hydrolysis with enzyme was improved effectively at its optimum temperature and pH. 4. The hydrolysis of stratum corneum with enzyme increased by the addition of surfactants. The order of compatibility with enzyme was in the order of Triton X-100>AOS>LAS.

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TDP(time-dependent parameters)를 적용하여 분석한 자율신경계 반응에 의한 감성인식에 대한 연구 (The study on emotion recognition by time-dependent parameters of autonomic nervous response)

  • 김종화;황민철;김영주;우진철
    • 감성과학
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    • 제11권4호
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    • pp.637-644
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    • 2008
  • 감성을 인식하고 반응하는 감성컴퓨터를 개발하기 위한 감성인식에 대한 연구가 활발하다. 본 연구는 2차원으로 정의한 감성을 측정하기 위해서 시간에 따른 생리신호의 반응 변수를 정의하고 그 변수로 인한 감성을 구분하고자 하였다. 10명의 대학생에게 16장의 감성을 유발시킬 수 있는 이미지를 제시하였다. 그리고 자율신경계 반응으로 ECG(electrocardiogram), PPG(photoplethysmogram), GSR(Galvanic skin response), RSP (respiration), 그리고 SKT(skin temperature)를 측정하였다. 본 연구는 자율신경계 반응에 대해 Delay, Activation, Half recovery 그리고 Full recovery로 이루어진 TDP(Time Dependent Parameters)를 정의하고 감성을 구분할 수 있는지에 대한 통계적 유의성을 검증하였다. 그 결과, TDP가 감성을 구분할 수 있는 유의미한 변수로 확인하였고 특징적 경향을 보임을 알 수 있었다. 그러므로 본 연구는 TDP가 감성 인식을 위한 유효한 변수일 가능성을 보였다.

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Changes in Quality Characteristics of Pork Patties Containing Antioxidative Fish Skin Peptide or Fish Skin Peptide-loaded Nanoliposomes during Refrigerated Storage

  • Bai, Jing-Jing;Lee, Jung-Gyu;Lee, Sang-Yoon;Kim, Soojin;Choi, Mi-Jung;Cho, Youngjae
    • 한국축산식품학회지
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    • 제37권5호
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    • pp.752-763
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    • 2017
  • Marine fish skin peptides (FSP) have been widely studied due to their antioxidant and antimicrobial properties. We aimed to use a natural antioxidant, FSP, to replacing synthetic preservatives in a pork patty model, which is safer for human body. Moreover, nano-liposome technology can be applied for masking the fishy smell and improving the stability of this peptide. Therefore, in this study, the effects of FSP and FSP-loaded liposomes (FSPL) on pork patty were evaluated through the tests of thiobarbituric acid reactive substances (TBARS), color, cooking loss, texture, volatile basic nitrogen (VBN), and the pH value, during 14 d of refrigerated ($4^{\circ}C$) storage. The results showed that all FSP-treated patties had lower TBARS values than control patties, which indicated an inhibitory effect of FSP on lipid oxidation. This effect in the patties depended on the FSP concentration. However, FSPL-treated patties showed significantly higher and undesirable TBARS values compared to the control, and this effect depended on the FSPL concentration. None of the physicochemical results showed remarkable changes except the pH and VBN values. Therefore, this study provides evidence that FSP has great potential to inhibit the lipid oxidation of pork patties and is capable of maintaining the quality and extending the shelf life. However, it is necessary to study the application of FSP treatments greater than 3% to improve the antioxidant effect on pork patties and search for other coating materials and technology to reduce the drawbacks of FSP.

Phototoxicity: Its Mechanism and Animal Alternative Test Methods

  • Kim, Kyuri;Park, Hyeonji;Lim, Kyung-Min
    • Toxicological Research
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    • 제31권2호
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    • pp.97-104
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    • 2015
  • The skin exposure to solar irradiation and photoreactive xenobiotics may produce abnormal skin reaction, phototoxicity. Phototoxicity is an acute light-induced response, which occurs when photoreacive chemicals are activated by solar lights and transformed into products cytotoxic against the skin cells. Multifarious symptoms of phototoxicity are identified, skin irritation, erythema, pruritis, and edema that are similar to those of the exaggerated sunburn. Diverse organic chemicals, especially drugs, are known to induce phototoxicity, which is probably from the common possession of UV-absorbing benzene or heterocyclic rings in their molecular structures. Both UVB (290~320 nm) and UVA (320~400 nm) are responsible for the manifestation of phototoxicity. Absorption of photons and absorbed energy (hv) by photoactive chemicals results in molecular changes or generates reactive oxygen species and depending on the way how endogenous molecules are affected by phototoxicants, mechanisms of phototoxcity is categorized into two modes of action: Direct when unstable species from excited state directly react with the endogenous molecules, and indirect when endogeneous molecules react with secondary photoproducts. In order to identify phototoxic potential of a chemical, various test methods have been introduced. Focus is given to animal alternative test methods, i.e., in vitro, and in chemico assays as well as in vivo. 3T3 neutral red uptake assay, erythrocyte photohemolysis test, and phototoxicity test using human 3-dimensional (3D) epidermis model are examples of in vitro assays. In chemico methods evaluate the generation of reactive oxygen species or DNA strand break activity employing plasmid for chemicals, or drugs with phototoxic potential.

피부미용 종사자의 그릿이 자기효능과 직무성과에 미치는 영향: 자기관리의 매개효과 분석 (Analysis of Mediating Effect of Skin Care Self-Management in the Relationship between Self-Efficacy and Business Performance of Skin Care Workers' Grit)

  • 김규랑
    • 한국응용과학기술학회지
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    • 제40권6호
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    • pp.1506-1520
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    • 2023
  • 본 연구의 목적은 피부미용 종사자의 그릿이 자기효능감과 직무성과에 미치는 영향을 관계에서 자기관리의 매개효과를 실증적으로 분석하는데 있다. 본 연구의 연구참여자는 서울·경기 스킨케어샵과 병원 종사자 344명을 대상으로 구조화된 설문지를 통하여 자료를 수집하였다. 수집된 자료는 SPSS와 AMOS 26.0 통계 프로그램을 이용하여 기술통계, 확인적 요인분석, 상관관계, 구조방정식모델, bootstrapping기법을 이용한 매개효과 분석을 통하여 자료를 분석하였다. 일련의 연구절차를 통하여 도출된 결론은 다음과 같다. 첫째, 피부미용 종사자의 그릿은 자기관리, 자기효능감, 직무성과에 유의미한 정의(+)영향 관계를 나타내었다. 둘째, 피부미용 종사자의 자기관리는 자기효능감과 직무성과에 유의미한 정의(+) 영향 관계를 나타내었다. 셋째, 피부미용 종사자의 그릿과 직무성과와의 관계에서 자기관는 유의미한 매개효과가 있는 것으로 나타났다. 따라서 뷰티 산업 종사자들의 그릿 함양을 통해 자기관리, 자기효능감 및 직무성과를 높일 수 있는 인적관리와 교육 프로그램의 적용이 절실히 필요하다고 판단된다..

피부투과 기능성 펩타이드를 이용한 경피투과성 상피세포성장인자의 개발 (Development of Dermal Transduction Epidermal Growth Factor (EGF) Using A Skin Penetrating Functional Peptide)

  • 강진선;나하나;박선욱;엄효정;이병규;신희제
    • 대한화장품학회지
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    • 제45권2호
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    • pp.175-184
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    • 2019
  • 상피세포성장인자(epidermal growth factor, EGF)는 인간의 표피 및 진피에서 세포막 수용체와 상호작용을 통하여 세포의 생장 및 증식을 유도하는 기능을 갖고 있다. 이 같은 EGF의 기능은 의료 및 화장품 분야에서 상처치유 의약품 및 노화방지 화장품의 주요원료로 사용되고 있다. 화장품 원료로서 EGF는 피부장벽으로 알려져 있는 피부 각질층의 투과가 잘 안되기 때문에 가지고 있는 본연의 효능을 구현하는 데 문제가 있다. 본 연구에서는 EGF의 경피투과 효율을 개선하기 위하여 피부 투과능이 확인된 거대분자 전송 도메인(macromolecule transduction domain, MTD) 151이 융합된 형태로 재조합 인간 상피세포성장인자 ($MTD_{151}-EGF$)를 개발하였다. $MTD_{151}-EGF$의 유전자가 coding된 vector로 형질전환된 대장균에서 $MTD_{151}-EGF$ 발현시킨 후 정제를 진행하였다. 정제된 MTD-EGF를 대상으로 세포증식시험, 세포독성시험, 생체외 피부흡수시험 그리고 인공피부를 이용한 경피투과능을 평가하였다. 99% 이상 고순도로 정제된 $MTD_{151}-EGF$의 세포증식 활성은 EGF 대비 동등 이상의 수준이었으며, 세포독성은 관찰되지 않았다. 또한, 인공피부 투과모델에서 FITC로 표지된 EGF와 $MTD_{151}-EGF$의 진피층까지의 투과를 공초점 현미경으로 관찰한 결과, $MTD_{151}-EGF$는 EGF 대비 우수한 투과능을 보였으며, 경피흡수 시스템을 이용한 투과물질의 정량분석 결과, EGF 대비 약 16 배 이상 투과량이 많은 것으로 확인되었다. 이러한 결과들은 다양한 활성물질들의 화장품용 원료로서의 경피투과에 MTD가 기존의 물리적인 경피투과 방법을 효율적으로 개선한 대안이 될 것으로 판단된다.

Development of in vitro 3D hair growth model using tissue engineering technology

  • Park, Jung-Keug
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2003년도 생물공학의 동향(XIII)
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    • pp.113-117
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    • 2003
  • 모낭은 복잡한 상호작용을 통하여 성장 및 주기가 조절되며 다양한 세포들로 구성진 기관이다. 본 연구에서는 조직공학적 방법을 이용하여 모낭을 구성하는 세포들을 분리 및 배양하였고 세포들의 형태와 증식양상을 관찰할 수 있었고, 기관배양의 방법을 통하여 in vitro에서 첨가물에 대한 모발의 성장 및 주기의 변화를 살펴보았다.

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밝기 변화를 고려한 색상과 채도의 확률 모델에 기반한 조명변화에 간인한 컬러분할 (Color Segmentation robust to Illumination Variations based on Statistical Methods of Hue and Saturation including Brightness)

  • 김치호;유범재;김학배
    • 대한전기학회논문지:시스템및제어부문D
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    • 제54권10호
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    • pp.604-614
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    • 2005
  • Color segmentation takes great attentions since a color is an effective and robust visual cue for characterizing one object from other objects. Color segmentation is, however, suffered from color variation induced from irregular illumination changes. This paper proposes a reliable color modeling approach in HSI (Hue-Saturation-Intensity) rotor space considering intensity information by adopting B-spline curve fitting to make a mathematical model for statistical characteristics of a color with respect to brightness. It is based on the fact that color distribution of a single-colored object is not invariant with respect to brightness variations even in HS (Hue-Saturation) plane. The proposed approach is applied for the segmentation of human skin areas successfully under various illumination conditions.

Face detection using active contours

  • Chang, Jae-Sik;Lee, Mu-Youl;Moon, Chae-Hyun;Park, Hye-Sun;Lee, Kyung-Mi;Kim, Hang-Joon
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2002년도 ITC-CSCC -3
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    • pp.1515-1518
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    • 2002
  • This paper proposes an active contour model to detect facial regions in a given image. Accordingly we use the color information human faces which is represented by a skin color model. We evolve the active contour using the level set method which allows for cusps, corners, and automatic topological changes. Experimental results show the effectiveness of the proposed method.

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