• BMB Reports
• /
• v.56 no.1
• /
• pp.32-42
• /
• 2023

Heart disease is one of the major life-threatening diseases with high mortality and incidence worldwide. Several model systems, such as primary cells and animals, have been used to understand heart diseases and establish appropriate treatments. However, they have limitations in accuracy and reproducibility in recapitulating disease pathophysiology and evaluating drug responses. In recent years, three-dimensional (3D) cardiac tissue models produced using tissue engineering technology and human cells have outperformed conventional models. In particular, the integration of cell reprogramming techniques with bioengineering platforms (e.g., microfluidics, scaffolds, bioprinting, and biophysical stimuli) has facilitated the development of heart-on-a-chip, cardiac spheroid/organoid, and engineered heart tissue (EHT) to recapitulate the structural and functional features of the native human heart. These cardiac models have improved heart disease modeling and toxicological evaluation. In this review, we summarize the cell types for the fabrication of cardiac tissue models, introduce diverse 3D human cardiac tissue models, and discuss the strategies to enhance their complexity and maturity. Finally, recent studies in the modeling of various heart diseases are reviewed.

• Structural Engineering and Mechanics
• /
• v.86 no.2
• /
• pp.237-247
• /
• 2023

Fourier series-based models in the time domain are frequently established to represent individual bouncing loads, which neglects the stochastic property of human bouncing activity. A power spectral density (PSD) model in the frequency domain for individual bouncing loads is developed herein. An experiment was conducted on individual bouncing loads, resulting in 957 records linked to form long samples to achieve a fine frequency resolution. The Welch method was applied to the linked samples to obtain the experimental PSD, which was normalized by the bouncing frequency and the harmonic order. The energy, energy distribution center, and energy distribution shape of the experimental PSD were investigated to establish the PSD model. The proposed model was used to analyze structural vibration responses using stochastic vibration theory, which was verified via field measurements. It is believed that this framework can evaluate the vibration capacity of structures excited by bouncing crowds, such as concert halls and grandstands.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.32 no.10
• /
• pp.1651-1658
• /
• 2008

This study was to decided the proper garment pressure level on the human body parts. Six volunteers (female: 30-40years) put on the same types of bands, a brief, and a non-woven gown. Garment pressure was measured in regular order with the elastic band on the human body parts such as the upper arm, the waist, the thigh, and the calf. At the same time, physiological responses such as the skin blood flow rate on 2 fingers, 7 different skin temperatures, rectal temperature, heat rates, and subjective responses about the pressure sensation, thermal sensation, and humidity sensation were measured and inquired. The results were as follows; 1. The thicker subcutaneous fat thickness, the higher the mean garment pressure on pressurizing the upper arm(p<.001). Also the thicker subcutaneous fat thickness. the thicker the upper arm circumference. 2. Heart rates increased pressured the upper arm and decreased pressured the waist, the thigh, and the calf. The higher the garment pressure, the higher heart rates on all body parts were pressured. Especially lean subjects showed higher physiological load than others. 3. On pressurizing the upper arm, heart rates, rectal temperature, and mean skin temperature were higher than without pressured state and pressured other body parts.4. The proper garment pressure levels were decided 30gf/$cm^2$ for fat people, 20gf/$cm^2$ for others on the upper arms and 24gf/$cm^2$ on the calf.

• Fashion & Textile Research Journal
• /
• v.16 no.1
• /
• pp.167-174
• /
• 2014

The physical properties of textile materials and thermal physiological responses of the human subjects were evaluated with 4 different types of the army combat uniforms including US, German, Korean and general uniforms for this study. 8 male adults were used as the human subjects and the tests were done in the environmental chamber that was $25{\pm}0.1^{\circ}C$ of temperature, $65{\pm}5%$ of relative humidity and below 0.3 m/sec of air velocity. The test protocol consisted the rest period for 20 min., the exercise period for 20 min., the rest period for 20 min., the exercise period under wind condition for 20 min., and the recovery period for 20 min. The human subjects walked with 4.5 km/hr for 10 min., ran 7.5 km/hr for 10 min. during the first exercise period and walked and ran with the same speeds under 3.5 m/sec of the air velocity that simulated outdoor condition during the second exercise period. The test results of the study were as follows; The wind condition affected the skin and microclimate temperature of the human subjects lower compared to without wind condition, but had insignificant effect on the humidity control. The low air permeability of Korean uniform caused blocking the elimination of the humidity from the body and the regulation of body temperature. However, Korean uniforms could be the excellent one with the designs considering the ventilation of the uniforms and the textile fabrics with better air permeability.

• Molecules and Cells
• /
• v.44 no.9
• /
• pp.647-657
• /
• 2021

As a pancreatic inflammatory marker, regenerating islet-derived protein 3A (Reg3A) plays a key role in inflammation-associated pancreatic carcinogenesis by promoting cell proliferation, inhibiting apoptosis, and regulating cancer cell migration and invasion. This study aimed to reveal a novel immuno-regulatory mechanism by which Reg3A modulates tumour-promoting responses during pancreatic cancer (PC) progression. In an in vitro Transwell system that allowed the direct co-culture of human peripheral blood-derived dendritic cells (DCs) and Reg3A-overexpressing/ silenced human PC cells, PC cell-derived Reg3A was found to downregulate CD80, CD83 and CD86 expression on educated DCs, increase DC endocytic function, inhibit DC-induced T lymphocyte proliferation, reduce IL-12p70 production, and enhance IL-23 production by DCs. The positive effect of tumour-derived Reg3A-educated human DCs on PC progression was demonstrated in vivo by intraperitoneally transferring them into PC-implanted severe combined immunodeficiency (SCID) mice reconstituted with human T cells. A Reg3A-JAK2/STAT3 positive feedback loop was identified in DCs educated with Reg3A. In conclusion, as a tumour-derived factor, Reg3A acted to block the differentiation and maturation of the most important antigen-presenting cells, DCs, causing them to limit their potential anti-tumour responses, thus facilitating PC escape and progression.

• Clinical and Experimental Vaccine Research
• /
• v.12 no.4
• /
• pp.328-336
• /
• 2023

Purpose: Human peripheral blood mononuclear cell (PBMC)-based in vitro systems can be of great value in the development and assessment of vaccines but require the right medium for optimal performance of the different cell types present. Here, we compare three commonly used media for their capacity to support innate and adaptive immune responses evoked in PBMCs by Toll-like receptor (TLR) ligands and whole inactivated virus (WIV) influenza vaccine. Materials and Methods: Human PBMCs were cultured for different periods of time in Roswell Park Memorial Institute (RPMI), Dulbecco's minimal essential medium (DMEM), or Iscove's modified DMEM (IMDM) supplemented with 10% fetal calf serum. The viability of the cells was monitored and their responses to TLR ligands and WIV were assessed. Results: With increasing days of incubation, the viability of PBMCs cultured in RPMI or IMDM was slightly higher than that of cells cultured in DMEM. Upon exposure of the PBMCs to TLR ligands and WIV, RPMI was superior to the other two media in terms of supporting the expression of genes related to innate immunity, such as the TLR adaptor protein gene MyD88 (myeloid differentiation factor 88), the interferon (IFN)-stimulated genes MxA (myxovirus resistance protein 1) and ISG56 (interferon-stimulated gene 56), and the leukocyte recruitment chemokine gene MCP1 (monocyte chemoattractant protein-1). RPMI also performed best with regard to the activation of antigen-presenting cells. As for adaptive immunity, when stimulated with WIV, PBMCs cultured in RPMI or IMDM contained higher numbers of IFNγ-producing T cells and secreted more immunoglobulin G than PBMCs cultured in DMEM. Conclusion: Taken together, among the different media assessed, RPMI was identified as the optimal medium for a human PBMC-based in vitro vaccine evaluation system.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2007.04a
• /
• pp.30-49
• /
• 2007

• Journal of the Korean Society of Industry Convergence
• /
• v.1 no.2
• /
• pp.65-74
• /
• 1998

The purpose of this paper is to investigate the mount of relief of human solar heat load and thermal comfort in outdoor environment in summer, Six different types of sites, T garden and its neighboring area in Japan, were selected as the experiment sites. The experiments were conducted from 22 to 29 August, 1994 to find the relationship between climatic conditions and human responses, Climatic conditions, subjects's thermal sensation and skin temperature were measured. Radiant heat exchange on the human body was estimated on the basis of the measured air and surface temperature and solar radiation. Thermal index Operative Temperature and New Effective Temperature was modified with the effect of the radiant heat exchange. Human thermal comfort and skin temperature is affected by the solar radiation and the sky factor in an outdoor environment. The effect of tree shade was verified on thermal comfort, The mount of relief of human solar heat load is relation to the existence of shade a solar radiation and the sky factor. The urban garden is one of the effective design element in an urban environmental planning.

• Journal of the Korean Society of Safety
• /
• v.36 no.3
• /
• pp.60-65
• /
• 2021

Human error is often in part in the cause of accidents and the result of various factors in an organization. Accidents should be investigated to elucidate all causes. Therefore, to reduce accidents, it is necessary to identify which factors affect human error within the organization. In this study, five groups of influencing factors on human error were selected using previousresearch, and operational definitions were made based on them. In addition, a questionnaire for measuring latent variables by operational definition was developed as an observation variable, and responses were received from employees of chemical companies in Ulsan. Based on SEM (structural equation modeling) analysis, 1) confirmatory factor analysis of variables in the human error model, 2) reliability and validity of latent variables, 3) correlations among latent variables, 4) influencing coefficients among influence factors, and 5) the verification results of the paths that these influencing factors have on human error are introduced in this study.

• Culinary science and hospitality research
• /
• v.22 no.6
• /
• pp.132-144
• /
• 2016

This study reviewed previous researches about the competence selection of foodservice firms, and shows firm's performance model through the results. The study classified factors according to core competence, differentiation strategy, and management performance. Out of 400 survey responses from by the firm's executive and employees who had worked for over three years at the headquarters (sales, financial, marketing/plan, R & D, etc.), a total of 302 questionnaires were used for the final analysis due to missing values and biased responses (response rate: 75.5%). As the results of analyzing final research model of this study, it appeared that ${\chi}^2(df=170)=384.88$, ${\chi}^2/df=2.26$, GFI=0.90, NFI=0.92, CFI=0.95, RMSEA=0.07. The results indicated that the CEO leadership, organizational culture, and human resource competencies are a driving force in all aspects of competitive advantage differentiation strategies. In addition, the R & D innovation, service, and marketing differentiation strategies are positively related to performance. The results validate the fact that foodservice firms could reinforce strategic decisions through a variety core competencies and achieve continuous performance through competitive strategies.