• IMMUNE NETWORK
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• v.6 no.4
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• pp.185-191
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• 2006

Background: Based on outstanding progresses in animal experiments, vaccines for some human tumors have been developed. However, clinical effects of these vaccines have been far below than expected. This discrepancy might come from differences between animal models and human patients with respect to immunocompetency. The immune status of mice after tumor inoculation has not been well studied, which make us cautious in interpreting and applying the results from mice to human. We evaluated cell-mediated immune responses in mice after tumor cell inoculation. Methods: Mice were inoculated with TA3Ha, CT26, or 4T1. Delayed-type hypersensitivity (DTH) responses were induced 2-4 weeks after inoculation using 2,4-dinitro-1-fluorobenzene as an antigen. The relationships between the severity of DTH responses and the duration of tumor inoculation or the size of tumor mass were analyzed. Results: In T A3Ha groups, DTH response was elevated 2 weeks after inoculation, but depressed after 4 weeks, compared to the control group. When analyzed based on the sizes of tumor masses elicited, DTH responses were inversely related to the mass size, especially in those greater than 10 mm in diameter. In CT26 groups, while the duration after inoculation did not affect the severity of DTH responses, those with large mass showed depressed responses regardless the duration of inoculation. 4T1 cells grew so slowly that the size of tumor mass was small even 4 weeks after inoculation, and this group showed much higher DTH responses compared to that of tumor-free group. Conclusion: At least in an experimental setting where tumor model was induced by inoculating tumor cell lines into immunologically competent mice, the host immune response was elevated in early stage, and then depressed in late stage when the mass grew over a critical size.

• The Journal of the Korean Society for Microbiology
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• v.20 no.1
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• pp.45-53
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• 1985

This study was undertaken to assess the susceptibility of pigmented and nonpigmented strains of Serratia marcescens to antibiotics and human sera, and the effect of culture filtrates from pigmented and nonpigmented of Serratia marcescens on humoral and cellular immune responses in mice to thymus-dependent and indepependent antigens. Humoral immune response was measured by hemagglutinin (HA) and hemolysin (HE) to sheep red blood cell (SRBC), and Arthus or antibody response to polyvinylpyrrolidone (PVP). The cellular immune response was measured by delayed-type hypersensitivity (DTH) determined by footpad swelling reactin to SRBC. The resistance of pigmented strains of Serratia marcescens to the bactericidal action of heat inactivated human serum was insignificantly greater than that of nonpigmented strains. However, the pigmented strains were significantly more resistant to the bactericidal action of heat-untreated human serum than that of nonpigmented strains. The clinical isolates of Serratia marcestens was also tested for their resistance to several antibiotics. There was no difference between the pigmented and non-pigmented strains in the resistance to carbenicillin. However, nonpigmented strains were more resistant to gentamicin, kanamycin and tobramycin than the pigmented strains. The intraperitoneal administration of culture filtrates from the pigmented or nonpigmented strains into mice caused enhancemented of antibody response to SRBC or PVP, and of DTH to SRBC. Besides, their enhancement of immune responses was more prominent when culture filtrate from the pigmented strains was administered.

• Nutrition Research and Practice
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• v.7 no.5
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• pp.352-358
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• 2013

Korean pine nut oil (PNO) has been reported to have favorable effects on lipid metabolism and appetite control. We investigated whether PNO consumption could influence weight gain, and whether the PNO-induced effect would result in an improvement of immune function in high-fat diet (HFD)-induced obese mice. C57BL/6 mice were fed control diets with 10% energy fat from either PNO or soybean oil (SBO), or HFDs with 45% energy fat from 10% PNO or SBO and 35% lard, 20% PNO or SBO and 25% lard, or 30% PNO or SBO and 15% lard for 12 weeks. The proliferative responses of splenocytes upon stimulation with concanavalin A (Con A) or lipopolysaccharide (LPS), Con A-stimulated production of interleukin (IL)-2 and interferon (IFN)-${\gamma}$, and LPS-stimulated production of IL-6, IL-$1{\beta}$, and prostaglandin $E_2$ ($PGE_2$) by splenocytes were determined. Consumption of HFDs containing PNO resulted in significantly less weight gain (17% less, P < 0.001), and lower weight gain was mainly due to less white adipose tissue (18% less, P = 0.001). The reduction in weight gain did not result in the overall enhancement in splenocyte proliferation. Overall, PNO consumption resulted in a higher production of IL-$1{\beta}$ (P = 0.04). Replacement of SBO with PNO had no effect on the production of IL-2, IFN-${\gamma}$, IL-6, or $PGE_2$ in mice fed with either the control diets or HFDs. In conclusion, consumption of PNO reduced weight gain in mice fed with HFD, but this effect did not result in the overall improvement in immune responses.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.41 no.6
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• pp.43-49
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• 2004

Electric pulp test is a method to examine the vitality of dental pulp using physical and chemical stimulation. During the pulp test, the current stimulates intradental nerve, and it makes patients painful. In this paper, we measured each activating response EMG in anterior belly of digastric muscle, voice, and finger movement during the pulp test by increasing stimulus intensity gradually. We also measured excessive stimulus time from the activating responses (EMG, voice, and finger movement) to the end of the stimulation. We measured and analyzed excessive stimulus time for each stimulus detecting method. As a result, we developed automatic stimulus breaker using the human responses to stimulus during electric pulp test. We reduced the excessive stimulus time by disconnecting the pulp tester stimulus output rapidly in 10 ms after activating human response.

• Molecules and Cells
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• v.21 no.1
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• pp.121-128
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• 2006

Maitotoxin (MTX) is known as one of the most potent marine toxins involved in Ciguatera poisoning, but intracellular signaling pathways caused by MTX was not fully understood. Thus, we have investigated whether intracellular reactive oxygen species (ROS) are involved in MTX-induced cellular responses in human umbilical vein endothelial cells. MTX induced a dose-dependent increase of intracellular [$Ca^{2+}$]. MTX stimulated the production of intracellular ROS in a dose- and time-dependent manner, which was suppressed by BAPTA-AM, an intracellular $Ca^{2+}$ chelator. Ionomycin also elevated the ROS production in a dose-dependent manner. MTX elevated transamidation activity in a time-dependent manner and the activation was largely inhibited by transfection of tissue transglutaminase siRNA. The activation of tissue transglutaminase and ERK1/2 by MTX was suppressed by BAPTA-AM or ROS scavengers. In addition, MTX-induced cell death was significantly delayed by BAPTA-AM or a ROS scavenger. These results suggest that [$Ca^{2+}$]-dependent generation of intracellular ROS, at least in part, play an important role in MTX-stimulated cellular responses, such as activation of tTGase, ERK phosphorylation, and induction of cell death, in human umbilical vein endothelial cells.

• Biomaterials and Biomechanics in Bioengineering
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• v.2 no.3
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• pp.143-157
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• 2015

Successful integration of cementless femoral stems using porous surfaces relies on effective periimplant bone healing to secure the bone-implant interface. The initial stages of the healing process involve protein adsorption, fibrin clot formation and cell osteoconduction onto the implant surface. Modelling this process in vitro, the current work considered the effect of fibrin deposition on the responses of human mesenchymal stromal cells cultured on ferritic fibre networks intended for magneto-mechanical actuation of in-growing bone tissue. The underlying hypothesis for the study was that fibrin deposition would support early stromal cell attachment and physiological functions within the optimal regions for strain transmission to the cells in the fibre networks. Highly porous fibre networks composed of 444 ferritic stainless steel were selected due to their ability to support human osteoblasts and mesenchymal stromal cells without inducing untoward inflammatory responses in vitro. Cell attachment, proliferation, metabolic activity, differentiation and penetration into the ferritic fibre networks were examined for one week. For all fibrin-containing samples, cells were observed on and between the metal fibres, supported by the deposited fibrin, while cells on fibrin-free fibre networks (control surface) attached only onto fibre surfaces and junctions. Initial cell attachment, measured by analysis of deoxyribonucleic acid, increased significantly with increasing fibrinogen concentration within the physiological range. Despite higher cell numbers on fibrin-containing samples, similar metabolic activities to control surfaces were observed, which significantly increased for all samples over the duration of the study. It is concluded that fibrin deposition can support the early attachment of viable mesenchymal stromal cells within the inter-fibre spaces of fibre networks intended for magneto-mechanical strain transduction to in-growing cells.

• Journal of the Korean housing association
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• v.3 no.1
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• pp.85-92
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• 1992

A floor system have played a major part in the living in Korean house. It is an essential element in the residential houses. Uufortunately, a design method of the floor heating system for the human thereat comfort has not established in Korea system. This paper aims to review the previous researches to find the issue for the futher study of comfort floor heating design. The following results were obtained: 1) Nevins et at conducted experiments on the effect of the comfortable of floor and air temperatures on the psychological responses by a large number of subjects. Its objective was to find the combined condition to obtain thermal comfort for sedentary posture.7here has never been researches regarding a sitting Person on the heated floor. 2) Some investigation in Japan on the effect of floor heating system on a sitting person on the floor have been conducted for these years, but they are net based on the human heat balance but on only psychological responses. 3) Hirayama et al developed an indication method of the effect of heat conduction on the sedentary person.4) There are few researches on the human thermal comfort compared whth indoor environmental researches on mechanical controls in Korea. Thus, the further study on the followings in needed the estimaiing of heat conduction between tile hunman body and a floor, developing an index expressed the combined effect of convection, radiation and conduction and its associated experiments.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.33 no.1
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• pp.27-33
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• 2009

Human postural responses appeared to have stereotyped modality, such as ankle mode, knee mode and hip mode in response to various perturbations. We examined whether human postural control gain of full-state feedback could be decoupled along with the eigenvector. To verify the model, postural responses subjected to fast backward perturbation were used. Upright posture was modeled as 3-segment inverted pendulum incorporated with feedback control, and joint torques were calculated using inverse dynamics. Postural modalities such as ankle, knee and hip mode were obtained from eigenvectors of biomechanical model. As oppose to the full-state feedback control, independent eigenvector control assumes that modal control input is determined by the linear combination of corresponding modality. We used optimization method to obtain and compare the feedback gains for both independent eigenvector control and full-state feedback control. As a result, we found that simulation result of eigenvector feedback was not competitive in comparison with that of full-state feedback control. This implies that the CNS would make use of full-state body information to generate compensative joint torques.

• Journal of Families and Better Life
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• v.24 no.5 s.83
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• pp.223-237
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• 2006

This study explores how well parents and their children recognize the social attitudes of one another. Mothers, fathers and youths were asked to state their own opinion on various social issues then predict their children's, fathers' and mothers' responses(attributed attitudes). Empirical evaluation of the possible socialization consequences of actual versus attributed attitudes leads to a series of hypotheses. The data were collected from single students at a university in Seoul and their parents. Included in the seven social attitude were sexuality, educational, economic, political, ecological, religious and family issues. Analysis of the responses 98-110 triads, each consisting a mother, a father and a young adult child showed that both mothers and fathers were limited in their ability to gauge the attitudes of their children. Guided by attribution theory, this study tested several hypothesized relationships between the actual response of mother, the actual response of the father, the perceived response of the mother, the perceived response of the father and the actual response of the child. The theoretical model was tested with AMOS 5.0, utilizing path analysis, which is a form of structural equation modeling with manifest variables. Overall model fit was assessed by examining GFI, NFI, TLI, CFI and RMR. Results of the data analysis can be summarized as follows. First, the children perceived their mothers and fathers to be highly similar in their opinions and the actual responses of the mothers and the fathers were considerably correlated. Second, the fathers' responses whether attributed or actual were more predictive than the mothers' responses to their children's opinions. The alternative model suggests considerable support for the attribution theory. Indeed, within a family, the actual opinions of parents appear to have little direct bearing on the child's orientations, except when the actual orientations are perceived and reinterpreted by the children. It is not what parents think, but what their children think they think that predicts their offsprings' attitudes.

• International conference on construction engineering and project management
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• 2020.12a
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• pp.237-242
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• 2020

Construction noise is among the most critical stressors that adversely affect the quality of life of the people residing near construction sites. Many countries strictly regulate construction noise based on sound pressure levels, as well as timeslots and type of construction equipment. However, individuals react differently to noise, and their tolerance to noise levels varies, which should be considered when regulating construction noise. Although studies have attempted to analyze individuals' stress responses to construction noise, the lack of quantitative methods to measure stress has limited our understanding of individuals' stress responses to noise. Therefore, the authors proposed a quantitative stress measurement framework with a wearable electroencephalogram (EEG) sensor to decipher human brain wave patterns caused by diverse construction stressors (e.g., worksite hazards). This present study extends this framework to investigate the feasibility of using the wearable EEG sensor to measure individuals' emotional stress responses to construction noise in a laboratory setting. EEG data were collected from three subjects exposed to different construction noises (e.g., tonal vs. impulsive noises, different sound pressure levels) recorded at real construction sites. Simultaneously, the subjects' perceived stress levels against these noises were measured. The results indicate that the wearable EEG sensor can help understand diverse individuals' stress responses to nearby construction noises. This research provides a more quantitative means for measuring the impact of the noise generated at a construction site on neighboring communities, which can help frame more reasonable construction noise regulations that consider various types of residents in urban areas.