• Toxicological Research
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• v.11 no.1
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• pp.77-80
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• 1995

Antigenic potential of genetically-engineered human erythropoietin (EPO) was assessed in guinea pigs (active systemic anaphylaxis [ASA] ; passive cutaneous anaphylaxis [PCA]) and in vitro (hemagglutination test [PHA]). In ASA, EPO at 70~700 U/kg elicited a weak anaphylactic response tvhereas the positive control ovalbumin (OVA) did cause intensive responses leading to death in 40% animals. However, the extract of CHO cells, to which EPO gene was introduced, did not cause any symptom. In PCA and PHA tests, neither EPO nor CHO cell extract induced positive responses. OVA, in contrast, produced high titers in both PCA and PHA tests. It was concluded that, in light of the fact that EPO was slightly antigenic only in ASA but not in PCA or PHA and also that human EPO is a foreign protein to guinea pigs, the present EPO may not be antigenic in humans.

• Journal of the Ergonomics Society of Korea
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• v.22 no.3
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• pp.1-11
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• 2003

The objective of this research is the development of the Automatic Subjective Assessment System (ASAS). The proposed subjective assessment system is designed to evaluate human emotion and sensibility (or "gamsung" in Korean terminology) with subjective responses of volunteers about the experiment of emotion and sensibility. Once volunteers enter their subjective responses about the experiment into the developed system, the proposed system can automatically generate statistical results of human emotion and sensibility using Statistical Package for the Social Sciences (SPSS). Then, the system stores the statistical results in the database which will be open to public through internet. The proposed system will be integrated into the universal" gamsung" assessment system for evaluation of human emotion and sensibility.

• Journal of the Ergonomics Society of Korea
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• v.18 no.3
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• pp.1-12
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• 1999

This study investigated human operator's perceptual and psychophysical responses to vibrotactile stimulation on various parts of the hand. Using a small vibrotactile display, the effects of three mechanical parameters consisting vibrotactile stimulations, i.e., vibration frequency, pulse-width modulation duty cycle, and number of contactors, on differential thresholds were examined at five different loci of the hand. It was observed that differential threshold varies with vibration frequency and number of active contactors. Differential sensitivity was the greatest at the vibration frequency of 120 Hz. The differential sensitivity was not found to be affected by loci on the hand. The area of stimulation on the hand was also found to be significant in that the sensitivity increased with the number of active contactors. It should be noted that the conclusions from this study generally correspond to those from the previous study on the absolute sensitivity. which means that tactile sensitivity to vibrotactile stimulations can be controlled with a systematic and consistent passion for emulating normal everyday contact on human hands in teleoperation and virtual reality applications.

• IMMUNE NETWORK
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• v.9 no.2
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• pp.41-45
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• 2009

Activating and inhibitory cell surface receptors play important roles in regulation of immune responses. Recent progress has demonstrated that many inhibitory receptors pair with activating, as well as inhibitory, isoforms, both of whose genes are located in small clusters on a chromosome. We and others identified paired activating and inhibitory immunoglobulin-like receptors, designated myeloid-associated immunoglobulin-like receptors (MAIR) (CD300). MAIR is a multigene family consisting of nine genes on a small segment of mouse chromosome 11. MAIR family receptors are preferentially expressed on myeloid cells, including macrophages, dendritic cells, granulocytes, and bone-marrow-derived cultured mast cells, and a subset of B cells and regulate activation of these cells. Thus, MAIR plays an important role in innate immunity mediated by myeloid cells.

• Journal of Engineering Education Research
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• v.22 no.1
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• pp.16-21
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• 2019

This study deals with empathic concern in the age of the fourth industrial revolution. A study, investigating emotional and empathic responses towards a situation of negative human-robot interaction, was conducted using mobile surveys and self-reports. In total, 116 subjects were recruited regardless of their disciplinary and demographic backgrounds. For data analysis, a mixed method was used. Results show that people differ in emotional and empathic responses in human-robot interaction. This individual difference was influenced by educational backgrounds and gender. Suggestions for engineering education were discussed.

• The Korean Journal of Physiology and Pharmacology
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• v.20 no.5
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• pp.449-457
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• 2016

N-acetyl-L-cysteine (NAC) and cysteine have been implicated in a number of human neutrophils' functional responses. However, though $Ca^{2+}$ signaling is one of the key signalings contributing to the functional responses of human neutrophils, effects of NAC and cysteine on intracellular calcium concentration ($[Ca^{2+}]_i$) in human neutrophils have not been investigated yet. Thus, this study was carried out with an objective to investigate the effects of NAC and cysteine on $[Ca^{2+}]_i$ in human neutrophils. We observed that NAC ($1{\mu}M{\sim}1mM$) and cysteine ($10{\mu}M{\sim}1mM$) increased $[Ca^{2+}]_i$ in human neutrophils in a concentration-dependent manner. In NAC pre-supplmented buffer, an additive effect on N-formyl-methionine-leucine-phenylalanine (fMLP)-induced increase in $[Ca^{2+}]_i$ in human neutrophils was observed. In $Ca^{2+}$-free buffer, NAC- and cysteine-induced $[Ca^{2+}]_i$ increase in human neutrophils completely disappeared, suggesting that NAC- and cysteine-mediated increase in $[Ca^{2+}]_i$ in human neutrophils occur through $Ca^{2+}$ influx. NAC- and cysteine-induced $[Ca^{2+}]_i$ increase was effectively inhibited by calcium channel inhibitors SKF96365 ($10{\mu}m$) and ruthenium red ($20{\mu}m$). In $Na^+$-free HEPES, both NAC and cysteine induced a marked increase in $[Ca^{2+}]_i$ in human neutrophils, arguing against the possibility that $Na^+$-dependent intracellular uptake of NAC and cysteine is necessary for their $[Ca^{2+}]_i$ increasing activity. Our results show that NAC and cysteine induce $[Ca^{2+}]_i$ increase through $Ca^{2+}$ influx in human neutrophils via SKF96365- and ruthenium red-dependent way.

• IMMUNE NETWORK
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• v.21 no.6
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• pp.42.1-42.20
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• 2021

Eosinophils play critical roles in the maintenance of homeostasis in innate and adaptive immunity. Although primarily known for their roles in parasitic infections and the development of Th2 cell responses, eosinophils also play complex roles in other immune responses ranging from anti-inflammation to defense against viral and bacterial infections. However, the contributions of pattern recognition receptors in general, and NOD-like receptors (NLRs) in particular, to eosinophil involvement in these immune responses remain relatively underappreciated. Our in vivo studies demonstrated that NLRC4 deficient mice had a decreased number of eosinophils and impaired Th2 responses after induction of an allergic airway disease model. Our in vitro data, utilizing human eosinophilic EoL-1 cells, suggested that TLR2 induction markedly induced pro-inflammatory responses and inflammasome forming NLRC4 and NLRP3. Moreover, activation by their specific ligands resulted in caspase-1 cleavage and mature IL-1β secretion. Interestingly, Th2 responses such as secretion of IL-5 and IL-13 decreased after transfection of EoL-1 cells with short interfering RNAs targeting human NLRC4. Specific induction of NLRC4 with PAM3CSK4 and flagellin upregulated the expression of IL-5 receptor and expression of Fc epsilon receptors (FcεR1α, FcεR2). Strikingly, activation of the NLRC4 inflammasome also promoted expression of the costimulatory receptor CD80 as well as expression of immunoregulatory receptors PD-L1 and Siglec-8. Concomitant with NLRC4 upregulation, we found an increase in expression and activation of matrix metalloproteinase (MMP)-9, but not MMP-2. Collectively, our results present new potential roles of NLRC4 in mediating a variety of eosinopilic functions.

• Korean Journal of Child Studies
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• v.30 no.2
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• pp.145-159
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• 2009

In this study, mothers of 205 4- to 5-year-old preschoolers responded to aggression episodes of Werner et al. (2006); preschoolers' teachers responded to the Preschool Social Behavior Scale (Crick et al., 1997). Results showed, (1) boys exhibited more overt and relational aggression. (2) In overt aggression episodes, mothers used encouragement to boys and rule violation responses to girls; in relational aggression episodes, mothers used encouragement and power assertion responses to girls. (3) Mothers' power assertion about overt aggression related negatively with preschoolers' overt aggressive behaviors; mothers' discussion about relational aggression related positively with preschoolers' overt aggressive behaviors. Implications of these findings for the mothers' responses by aggression types were discussed in order in better understand preschooler's aggressive behaviors.

• Culinary science and hospitality research
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• v.14 no.4
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• pp.398-414
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• 2008

The purpose of this study is to analyze the effect of restaurant atmosphere on its image and customers' emotional responses and behavior. The results of this study indicated that perceived restaurant atmospheres had a significant effect on customers' emotional responses, and these emotional responses greatly influenced the image of a restaurant. Especially, the ambient and cleanliness factors of restaurant atmosphere influenced a restaurant image, and the positive image from those factors had a significant effect on customer behavior. The design and human factors of restaurant atmosphere influenced customer behavior, and the positive image from those factors had a significant effect on customer behavior. Finally, it was verified that the restaurant atmospheric factors affected its image and customers' emotional responses and behavior. Moreover, the better the restaurant atmospheric factors(design, ambient, cleanliness, humanity) are, the better customers' emotional responses and image are, thereby increasing customers' revisiting and word-of-mouth intention.

• Cartoon and Animation Studies
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• s.15
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• pp.179-192
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• 2009

This study investigates the effects of empathy responses towards animated spokes-characters in advertising on brand likability. This study also examines the mediating role of cognitive and affective responses towards character advertising in the effect process of empathy. Statistically, several significant results are found. First, empathy has positive effects on cognitive responses and affective responses. Second, both cognitive and affective responses have positive influence on brand likability, but the effects of cognitive responses show relatively weaker than those of affective responses. Third, empathy has a positive influence on brand likability mediated by cognitive responses, but affective responses as a mediator do not show statistically significant differences. The results of this study indicate consumers can have emotional responses to advertising characters just as they do to human information sources. And the results also imply that enhancement of the empathy intensity should be considered to make effective animated characters in advertising, and that advertising appeals should be practiced to form cognitive responses positively and strongly to strengthen consumers' empathy responses.