• Journal of Periodontal and Implant Science
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• 제35권1호
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• pp.21-30
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• 2005

Matrix metalloproteinases (MMPs) are a family of host-derived proteolytic enzymes and implicated in the remodeling and degradation of extracellular matrix under both physiological and pathological conditions. Connective tissue degradation in periodontal diseases is thought to be due to excessive MMP activities over their specific inhibitors. The effects of lipopolysaccharide (LPS) from Prevotella intermedia, one of the major putative pathogens of periodontitis, on the expression of mRNA for MMPs and tissue inhibitors of metalloproteinases (TIMPs) in human gingival and periodontal ligament fibroblasts were examined by reverse transcriptase-polymerase chain reaction (RT-PCR). The expression of mRNAs encoding MMP-1, -2, -3, -10, and -14 was increased in human gingival fibroblasts treated with p. intermedia LPS, whereas MMP-11 and TIMP-2 mRNA expression was decreased in these cells stimulated with LPS. P. intermedia LPS increased the MMP-1, -2, -10, -11, and -14 mRNA expression and decreased TIMP-1 and -2 mRNA expression in human periodontal ligament fibroblasts. These findings imply that P. intermedia LPS may play an important role in the connective tissue degradation in periodontitis.

• Journal of Photoscience
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• 제9권2호
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• pp.424-426
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• 2002

Recently, much attention has been paid to the physiological functions of flavonoids associated with their antioxidant properties. However, there was a lack of information on the molecular mechanism at which flavonoids play the antioxidative role. We have already studied on the oxidation of quercetin with hydrogen peroxide and sodium hypochlorite in alcoholic aqueous solution and determined the oxidation products. Through the structural analysis of the oxidation products, it was clarified that the hydroxyl group at C-3 in the C ring plays the important role in the antioxidative action of quercetin. Successively, rutin and (+)-catechin were oxidized with sodium hypochlorite and their mono- and di-chlorinated derivatives were obtained. These facts indicate that these flavonoids can directly scavenge hypochlorous acid and the active site in this scavenging reaction is not the hydroxyl group at C-3.

• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1505-1512
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• 2005

Bile salt deconjugation is the most biologically significant reaction among the bacterial alterations of bile acids in the gastrointestinal tract of human and animal. The responsible enzyme, bile salt hydrolase (BSH), catalyzes the hydrolysis of glycineand/or taurine-conjugated bile salts into amino acid residues and deconjugated bile acids. Herein we review current knowledge on the distribution of BSH activity among various microorganisms with respect to their biochemical and molecular characteristics. The proposed physiological impact of BSH activity on the host animal as well as on the BSH-producing bacterial cells is discussed. BSH activity of the probiotic strains is examined on the basis of BSH hypothesis, which was proposed to explain cholesterol-lowering effects of probiotics. Finally, the potential applications of BSH research are briefly discussed.

• 동의생리병리학회지
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• 제20권2호
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• pp.312-319
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• 2006

Lee je-ma emphasis human constitution and write DongYeuSuSebowon(東醫壽世保元). He describe Sasang constitution diseases with Treatise on Febrile Diseases(傷寒論). Exogenous febrile disease(傷寒) is a pyrexia disease. Therefor this paper is focus on the pyrexia at Soyinin(少陰人) among DongYeuSuSebowon. Soyinin(少陰人) is consider that body water loss is more important than fever in pyrexia disease. And body water loss is happens easily at exothermic reaction (sweating, diarrhea) and sequela of Purgative Prescription(瀉下法), Inducing Diaphoresis(發汗法), Diuresis(利小便法).

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1669-1673
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• 2008

In this study, we simulated the atrial arrhythmia numerically. By using electro-physiological model of atrial cell from Nygren et al. and applying reaction-diffusion partial differential equation, we simulated electrical conduction in atrium. A 3-D mesh system representing the human atrium was reconstructed from the surface geometry of atrium. We used a stimulus in the form of an archetype around pulmonary vessels in the left atrium to cause the atrial arrhythmia. The septal atrial tarchycardia was developed after the stimulus.

• 한국의류산업학회지
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• 제2권5호
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• pp.416-422
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• 2000

In this study, to find out the physiological reaction of the human body and the sensation of comfort when people are wearing sportswear which is made of waterproof breathable fabrics under general environmental conditions (temperature : $20{\pm}1^{\circ}C$, humidity : $60{\pm}5%RH$, air current : 0.1 m/sec) and rainy environmental conditions (temperature : $20{\pm}1^{\circ}C$, humidity : $60{\pm}5%RH$, air current : 0.1 m/sec, rainfall : 250 1/hr), we made an experiment with sportswear in an artificial climate chamber and studied the thermal physiological response and subjective sensation. Mean skin temperature of the subjects was low and had a big range of fluctuation in rainy environmental conditions of two condition. Temperature started to increase at the beginning of the exercise, reached the maximum at the 2nd level of the exercise and then started to decline. Rectal temperature showed a slighter increase and bigger range of fluctuation in general conditions than in rainy conditions. Except clothing micro climate in rainy conditions, temperature and humidity and their range of fluctuation around back were higher than those around chest. Humidity was high and had wide range of fluctuation in general conditions. Heart rate was 4.4 beats/min higher in general conditions. In subjective test on rainy conditions, the feeling of discomfort increased due to the raindrops fallen on the skin. Unlike that in general conditions, cold sensation increased and humidity sensation reached to the peak after the exercise. In wearing sportswear made of shape memory breathable waterproof fabric, controlling function over a small amount of heat and water was distinctive while it turned out to be not so comfortable over a large amount of heat and water. Through this, the limitation of shape memory breathable waterproof fabric was recognised.

• 대한의용생체공학회:의공학회지
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• 제18권4호
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• pp.485-491
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• 1997

본 논문은 각성상태에 다른 생리신호와 행위신호 및 주관적 평가의 상관관계에 대하여 나타내었다. Nz와 반응시간은 mKSS level 의 변화와 동일한 경향을 나타내는데 반하여 1분당 눈 깜박임 수는 앞의 두 가지 변수와 다른 경향을 나타내었다. 1분당 눈깜박임 수는 mKSS level 1에서 5까지는 낮은 변화율 갖고 mKSS level 7에서는 높은 변화율을 갖는 반면에 mKSS level 9에서는 이와 반대로 변화율이 급격히 감소한다. 피검자들은 서로다른 1분당 눈깜박임 수(EBR)를 가지나 EBR의 변화율은 비슷하였다. 그러므로 EBR의 변화율을 각성판정지표로 사용할 수 있음을 알 수 있었다. 반응시간 실험 결과로부터mKSS level 5이상부터 작업수행능력이 낮아짐을 알 수 있었고 false positive 와 false negative 가 mKSS level3부터 관찰되었으므로 효과적으로 각성제어를 위하여 mKSS level 3과 5사이에 각성상태를 향상시키기 위한 소리나 향기 등의 자극을 주어야 함을 알 수 있었다.

• 동의생리병리학회지
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• 제24권5호
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• pp.822-826
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• 2010

In order to validate the use of Faeces Trogopterori as an anti-inflammatory drug in the traditional Korean medicine, I have investigated the effect of water-soluble extract of F. Trogopterori (EFT) on the production of monocyte chemoattractant protein-1 (MCP-1), of which chemokine stimulates the migration of mononuclear cells, in human umbilical vein endothelial cells (HUVECs) stimulated with tumor necrosis factor-alpha. The extract inhibited dose-dependently MCP-1 production without its cytotoxic effect on HUVECs, as measured by enzyme-linked immunosorbent assay, and significantly decreased mRNA levels of MCP-1, as determined using reverse transcription polymerase chain reaction. These results suggest that F. Trogopterori may have therapeutic potential in the control of endothelial disorders caused by inflammation.

• Journal of Microbiology and Biotechnology
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• 제27권5호
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• pp.909-915
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• 2017

Bifidobacterium bifidum BGN4 (BGN4) has many proven beneficial effects, including antiallergy and anticancer properties. It has been commercialized and used in several probiotic products, and thus strain-specific identification of this strain is very valuable for further strain-dependent physiological study. For this purpose, we developed novel multiplex polymerase chain reaction (PCR) primer sets for strain-specific detection of BGN4 in commercial products and fecal samples of animal models. The primer set was tested on seven strains of B. bifidum and 75 strains of the other Bifidobacterium species. The BGN4-specific regions were derived using megaBLAST against genome sequences of various B. bifidum databases and four sets of primers were designed. As a result, only BGN4 produced four PCR products simultaneously whereas the other strains did not. The PCR detection limit using BGN4-specific primer sets was $2.8{\times}10^1CFU/ml$ of BGN4. Those primer sets also detected and identified BGN4 in the probiotic products containing BNG4 and fecal samples from a BGN4-fed animal model with high specificity. Our results indicate that the PCR assay from this study is an efficient tool for the simple, rapid, and reliable identification of BGN4, for which probiotic strains are known.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2013년도 춘계 종합학술대회 논문집
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• pp.361-362
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• 2013

As one of the steps toward understanding how the plant is well adapted to strongly saline habitats, the purple pigment compound that is accumulated in Suaeda japonica was extracted and characterized. The extracted pigment compound exhibited typical characteristics of betacyanin that were represented by water solubility, pH- and temperature-dependent color changes, sensitivity to light, UV-Vis spectra, and gel electrophoretic migration pattern. LC-MS of the extracted pigment compound showed the presence of two major protonated molecular ions ($[M+H]^+$) at m/z 651.1 and m/z 827.1. According to the DPPH assay, it was found to have an antioxidant activity that is linearly increased in proportion to the reaction time for up to 30 min, and the activity was comparable to that of control BHA at 9.0 mg/ml. The cytotoxic activity against several tumor cell lines was also examined following the MTT assay. The significant growth inhibitory effect was observed on two tumor cell lines, SW-156 (human kidney carcinoma) and HEC-1B (human endometrial adenocarcinoma). Probably, the pigment compound may function as an osmolyte to uphold halotolerant physiological processes in saline environment.