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Expression of mRNA for matrix metalloproteinases and tissue inhibitors of metalloproteinases in human gingival and periodontal ligament fibroblasts treated with lipopolysaccharide from Prevotella intermedia  

Kim, Sung-Jo (Department of Periodontology, College of Dentistry, Pusan National University)
Choi, Eun-Young (Department of Life Science, College of Natural Science, Silla University)
Choi, In-Soon (Department of Life Science, College of Natural Science, Silla University)
Lee, Ju-Youn (Department of Periodontology, College of Dentistry, Pusan National University)
Choi, Jeom-Il (Department of Periodontology, College of Dentistry, Pusan National University)
Kim, Chong-Kwan (Department of Periodontology, College of Dentistry, Yonsei University)
Publication Information
Journal of Periodontal and Implant Science / v.35, no.1, 2005 , pp. 21-30 More about this Journal
Abstract
Matrix metalloproteinases (MMPs) are a family of host-derived proteolytic enzymes and implicated in the remodeling and degradation of extracellular matrix under both physiological and pathological conditions. Connective tissue degradation in periodontal diseases is thought to be due to excessive MMP activities over their specific inhibitors. The effects of lipopolysaccharide (LPS) from Prevotella intermedia, one of the major putative pathogens of periodontitis, on the expression of mRNA for MMPs and tissue inhibitors of metalloproteinases (TIMPs) in human gingival and periodontal ligament fibroblasts were examined by reverse transcriptase-polymerase chain reaction (RT-PCR). The expression of mRNAs encoding MMP-1, -2, -3, -10, and -14 was increased in human gingival fibroblasts treated with p. intermedia LPS, whereas MMP-11 and TIMP-2 mRNA expression was decreased in these cells stimulated with LPS. P. intermedia LPS increased the MMP-1, -2, -10, -11, and -14 mRNA expression and decreased TIMP-1 and -2 mRNA expression in human periodontal ligament fibroblasts. These findings imply that P. intermedia LPS may play an important role in the connective tissue degradation in periodontitis.
Keywords
matrix metalloproteinases; tissue inhibitors of metalloproteinases; human gingival fibroblasts; human periodontal ligament fibroblasts; Prevotella intermedia LPS;
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