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Effects of Faeces Trogopterori on the Production of Chemokine in HUVECs  

Moon, Chang-Min (Department of Physiology, School of Oriental Medicine & Research Center of Traditional Korea Medicine, Wonkwang University)
Kwon, Kang-Beom (Department of Physiology, School of Oriental Medicine & Research Center of Traditional Korea Medicine, Wonkwang University)
Ryu, Do-Gon (Department of Physiology, School of Oriental Medicine & Research Center of Traditional Korea Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.5, 2010 , pp. 822-826 More about this Journal
Abstract
In order to validate the use of Faeces Trogopterori as an anti-inflammatory drug in the traditional Korean medicine, I have investigated the effect of water-soluble extract of F. Trogopterori (EFT) on the production of monocyte chemoattractant protein-1 (MCP-1), of which chemokine stimulates the migration of mononuclear cells, in human umbilical vein endothelial cells (HUVECs) stimulated with tumor necrosis factor-alpha. The extract inhibited dose-dependently MCP-1 production without its cytotoxic effect on HUVECs, as measured by enzyme-linked immunosorbent assay, and significantly decreased mRNA levels of MCP-1, as determined using reverse transcription polymerase chain reaction. These results suggest that F. Trogopterori may have therapeutic potential in the control of endothelial disorders caused by inflammation.
Keywords
extract of Faeces Trogopterori(EFT); human umbilical vein endothelial cells; macrophage chemoattractant protein-1;
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1 정 경, 송병기, 구본홍. 경희한의대논문집, 2(1):171-181, 1979.
2 Li, Q. Clinical and experimental study of faeces trogo the antistimulative and antineoplastic effects of radixginseng used in combination with Faeces. Planta. Zhongguo Zhong Yao Za Zhi. 16: 90-92, 1996.
3 Chang, M. An experimental study on the antistimulative and antineoplasic effects of radix ginseng used in combination with Faeces Trogopterus. Zhongguo Zhong Yao Za Zhi. 22: 694-696, 1997.
4 이장천 外. 藥用動物學. 서울, 醫聖堂, pp 583-586, 2002.
5 김창민, 신민교, 안덕균, 이경순 外. 中藥大辭典. 서울, 정담, pp 3945-3949, 1997.
6 Li, Q., Lu, W., Lu, X. Advances in the study of feces Trogopterori. Zhongguo Zhong Yao Za Zhi. 23(9):570-573, 1998.
7 辛民敎. 臨床本草學. 서울, 永林社, pp 728-729, 1997.
8 Wang, S., Zhai, S., Wang, Y., Wang, L. Effect of radix Ginseng-faeces Trogopterori combination on pharmacodynamics and effective chemical composition of radix Ginseng. Zhongguo Zhong Yao Za Zhi. 20(10):630-632, 1995.
9 Guo, G.H., Lu, Y.B., Song, L.F. Effects of compatibility of radix Ginseng with faeces Trogopterori on toxicity in experimental animals. Zhongguo Zhong Yao Za Zhi. 19(4):247-250, 1994
10 申佶求. 申氏本草學. 서울, 壽交社, pp 545-542, 1982.
11 Barry, O.P., Pratico, D., Savani, R.C., FitzGerald, G.A. Modulation of monocyte-endothelial cell interactions by platelet microparticles. J Clin Invest. 1;102(1):136-144, 1998.
12 Lukacs, N.W., Strieter, R.M., Elner, V.M., Evanoff, H.L., Burdick, M., Kunkel, S.L. Intercellular adhesion molecule-1 mediates the expression of monocyte-derived MIP-1 alpha during monocyte-endothelial cell interactions. Blood. 1;83(5):1174-1178, 1994.
13 Nemeth, Z.H., Deitch, E.A., Lu, Q., Szabo, C., Hasko, G. NHE blockade inhibits chemokine production and NF-kappaB activation in immunostimulated endothelial cells. Am J Physiol Cell Physiol. 283(2):C396-403, 2002.   DOI
14 Li, Y., Chi, L., Stechschulte, D.J., Dileepan, K.N. Histamine-induced production of interleukin-6 and interleukin-8 by human coronary artery endothelial cells is enhanced by endotoxin and tumor necrosis factor-alpha. Microvasc Res. 61(3):253-262, 2001.   DOI   ScienceOn
15 Taekema-Roelvink, M.E., Kooten, C., Kooij, S.V., Heemskerk, E., Daha, M.R. Proteinase 3 enhances endothelial monocyte chemoattractant protein-1 production and induces increased adhesion of neutrophils to endothelial cells by upregulating intercellular cell adhesion molecule-1. J Am Soc Nephrol. 12(5):932-940, 2001.
16 Peng, H.B., Spiecker, M., Liao, J.K. Inducible nitric oxide: an autoregulatory feedback inhibitor of vascular inflammation. J Immunol. 15;161(4):1970-1976, 1998.
17 Cuff, C.A., Schwartz, J., Bergman, C.M., Russell, K.S., Bender, J.R., Ruddle, N.H. Lymphotoxin alpha3 induces chemokines and adhesion molecules: insight into the role of LT alpha in inflammation and lymphoid organ development. J Immunol. 15;161(12):6853-6860, 1998.
18 Vanhee, D., Delneste, Y., Lassalle, P., Gosset, P., Joseph, M., Tonnel, A.B. Modulation of endothelial cell adhesion molecule expression in a situation of chronic inflammatory stimulation. Cell Immunol. 155(2):446-456, 1994.   DOI   ScienceOn