• 제목/요약/키워드: Human patch test

검색결과 43건 처리시간 0.022초

홍삼가수분해농축액(GS-E3D)의 피부 안전성 평가 (Skin Safety Evaluation of Pectin Lyase-modified Red Ginseng Extract (GS-E3D))

  • 표미경;이경희;차선우;박기용;이기무
    • 생약학회지
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    • 제49권3호
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    • pp.246-254
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    • 2018
  • Pectin lyase-modified red ginseng extract (GS-E3D) is a newly developed ginsenoside Rd-enriched ginseng extract. This study was designed to investigate the skin safety of GS-E3D. Single oral toxicity, single dermal toxicity, bovine corneal opacity and permeability (BCOP) assay, skin irritation test with $SkinEthic^{TM}$ human epidermis model, skin sensitization local lymph node assay, and human patch test, were examined. The oral and dermal $LD_{50}$ value of GS-E3D was over 2,000 mg/kg in rats. GS-E3D was identified as a non-irritant to skin in BCOP assay, human epidermis models, and patch test from the 32 human subjects. The skin sensitization potential of GS-E3D was less than 25% in local lymph node assay. These results indicate that GS-E3D can be used as a safe ingredient without adverse effects in various skin care products.

발효 전복 추출물의 피부자극에 관한 연구 (The Study on Skin 1st Patch Test of Fermented Hydrolysate from Abalone(Haliotis discus hannai Ino))

  • 이충우;홍경희;전용진
    • 한국산학기술학회논문지
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    • 제17권1호
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    • pp.421-424
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    • 2016
  • 본 연구에서 남해안산 전복으로 부터 얻어진 일반 열수 전복 추출물과 발효추출물의 화장품 원료로서의 사용가능성을 평가하고자 기본적인 피부 안전성 시험으로 추물물과 이를 이용한 액정크림 제형에서의 피부 일차 첩포 테스트를 수행하였다. 비교군으로 정제수, 1,3-부틸렌글리콜, 액정크림으로 하였고 시료군은 전복추출물과 전복추출물을 함유한 액정크림 제형이었다. 피부 일차 첩포 테스트 결과, 비교군과 시료군 모두 피부의 자극이 없는 것으로 나타났다. 그러므로 전복 추출물이 화장품 원료로서 피부 안전성을 확인하였다.

In vitro Alternatives to Skin Irritation Test

  • Shin, Dae-Sup;Kim, Dai-Byung;Ryu, Seung-Rel;Lee, Sun-Hee;Koh, Jae-Sook;Park, Won-Sae;Kim, Pu-Young
    • Biomolecules & Therapeutics
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    • 제3권3호
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    • pp.242-244
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    • 1995
  • In vitro cell culture system has been proposed as a promising alternative model to in vivo skin irritation test. These studies were performed to screen the cytotoxicity effects of surfactants using normal human skin fibroblasts. Cell membrane integrity assessed by the leakage of lactate dehydrogenase (LDH) and mitochondrial integrity by MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromides reduction test were affected in a dose dependent manner. The irritation potential of surfactants to human skin patch test, and the changes of capillary permeability by rabbit intradermal safety test were assessed as in vivo methods. Our results suggest that LDH leakage assay and MTT reduction test using cultured human fibroblasts could be predictive for the irritancy of various surfactants in human, and LDH assay is superior correlated with in vivo test (r=0.886) to MTT test with in vivotest (r=0.757).

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봉독의 인체 피부자극성 평가 (Evaluation on Skin Irritation of Bee Venom in Human)

  • 한은혜;임미경;이상호;박근태;박지은;임영희;심인숙
    • 생약학회지
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    • 제48권3호
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    • pp.202-207
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    • 2017
  • The purpose of this study was to investigate the skin irritation of bee venom (melittin) in human. Skin irritation test was performed at 0.05% bee venom by 48 h single patch test in 30 healthy volunteers with no skin disease and skin sensitization test was performed by local lymph node assay in animal. In single patch test, 28 human subjects showed no reaction and 2 subjects showed 1+ or 3+ grade skin reaction. In a skin sensitization test of bee venom conducted using mice. no erythema was observed on the dorsal side of mice up to 8 days after application of bee venom on the skin. The results of the average stimulation index by ATP values showed that there was no irritation to the mice skin at 0.005% and 0.01% of bee venom. Therefore, bee venom is not likely to induce a significant skin irritation under 0.05% concentration.

Safety Evaluation of Topical Valproate Application

  • Choi, Sun Young;Seop, Song Yi;Hyun, Moo Yeol;Yoo, Kwang Ho;Kim, Beom Joon;Kim, Myeung Nam;Cho, Jae-We
    • Toxicological Research
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    • 제29권2호
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    • pp.87-90
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    • 2013
  • The potential role of topical valproate (VPA) in hair regrowth has been recently suggested. However, safety reports of VPA as a topical formulation are lacking. Therefore, in the present study, we investigated whether VPA causes skin irritation in humans. We first performed a cell viability test and showed that VPA did not exhibit toxicity toward HaCaT keratinocytes, fibroblasts, and RBL-3H mast cells. We then performed clinical patch test and skin irritation test through transdermal drug delivery with the help of microneedle rollers. No significant findings were obtained in the clinical patch test. In the skin irritation test, only 1 patient showed erythema at 1 hr, but the irritation reaction faded away within a few hours. Erythema and edema were not observed at 24 hr. We concluded that VPA has minimal potential to elicit skin irritation. Therefore, we consider that VPA can safely be applied to human skin.

The Synthesis and Safety of 3-Aminopropyl dihydrogen phosphate, a New Anti-aging Agent

  • Pyun Young Hoon;Ko
    • 대한화장품학회지
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    • 제22권2호
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    • pp.174-181
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    • 1996
  • The novel synthesis of 3-aminopropyl dihydrogen phosphate(3-APPA; 3-Aminopropane phosphoric acid), and its applicability to the skin as a cosmetic raw material in terms of its efficacy and toxicology were presented. The phosphorylation of 3-amino-1-propanol was carried out via cyclization into 6-membered 2, 6-oxaza-phosphoryl ring in the presence of phosphorous oxychloried and an organic base. The subsequent ring-opening hydrolysis and crystallization afforded the highly purified product in 90% isoloated yield. The method is much superior to the previous literature phosphorylation methodsm, as the procedure is simple and high-yielding. To confirm the efficacy of 3-APPA, several activities related to anti-aging capacity were measured. In-vitro human fibroblast, linear and 3-dimensional collagen matrix culture revealed that 3-APPA stimulated the proliferation of fibroblasts, and enhanced the synthesis of collagen, which showed 3-APPA's potency for skin wrinkle reduction. The toxicolgical aspect of 3-APPA was also extensively examined. In vivo toxicity tests such as acute oral toxicity, eye irritation, human patch, and the repeat insult human patch test proved 3-APPA to be a safe material. Thus 3-APPA can be used as an effective anti-aging agent for various cosmetic formulations.

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생체 표면에서의 물질 확산 측정을 위한 레이저 플라즈마 분광법 적용 (Measurement of Substance Diffusion on a Bio-body Surface Using Laser Plasma Spectroscopy)

  • 윤상우;오지헌;박성용;정재경;김주한
    • 한국기계가공학회지
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    • 제18권7호
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    • pp.71-76
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    • 2019
  • The diffusion of the drug component of the inflammatory patch into the living tissue was analyzed by laser induced plasma spectroscopy (LIBS). Calcium element, which is a diffusion catalyst of the drug in the inflammatory analgesic patch, is transferred into the body through the diffusion process of the substance. The test pieces used in the experiment are pig skin tissues which are similar to human skin. As a result, the diffusion coefficient D of the calcium element was found to be average $8.24{\times}10^{-2}({\mu}m^2/s)$. Experimental results showed that the most influential factors on the diffusion of materials were temperature variables.

항산화력이 우수한 Fructose 1,6-diphosphate(FDP)를 피부적용제로 응용하기 위한 안전성 평가 (The Safety Evaluation of a Potent Antioxidant, Fructose 1,6-diphosphate(FDP), for the Skin Application)

  • 김배환;이병석;정경미;안수미;안수선;심영철
    • Toxicological Research
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    • 제18권3호
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    • pp.267-273
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    • 2002
  • Fructose 1,6-diphosphate(FDP), a glycolytic metabolite, is reported to ameliorate inflammation and inhibit the nitric oxide production in murine macrophages stimulated with endotoxin. It is also reported that FDP has cytoprotective effects against hypoxia or ischemia/reperfusion injury in brain and heart, and may play a protective role in ultraviolet B (UVB, 280~320 nm)-injured keratinocyte by attenuating prostaglandin (PG)-E$_2$production and cyclooxygenase (COX)-2 expression, which are possibly through blocking the intracellular reactive oxygen species (ROS) accumulation. Therefore FDP is considered to act as a potent antioxidant especially in the skin. We conducted the several safety tests (single-dose toxicity, primary skin irritation test, eye irritation test, skin sensitization test, phototoxicity test, photosenitization test and human patch test) to see if FDP is safe in case used for the skin application. Our data obtained hitherto suggest that FDP is very safe if applied to the skin.

A mono-material tactile sensor with multi-sensing properties

  • Shida, Katsunori;Yuji, Junnichiro
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 1994년도 Proceedings of the Korea Automatic Control Conference, 9th (KACC) ; Taejeon, Korea; 17-20 Oct. 1994
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    • pp.587-592
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    • 1994
  • To realize artificial device with sensing ability of the human skin, a mono-material tactile sensor with three sensing functions made of some elastic thin electro-conductive rubber sheet with eight latticed patch elements is proposed. This trial sensor provides the information of three kinds of model material characteristics such as thermal property, hardness property and the surface situation of materials by setting up three kinds of surface models as test materials. It can be finally expected to estimate unknown model materials by analyzing the data of the sensor.

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Lactobiocin의 피부 염증 및 여드름 저해효과에 관한 연구 (Inhibitory activity of Lactobiocin on the skin inflammation and acnes)

  • 김광수;오세종;김기환;홍진천;이승화
    • 대한화장품학회지
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    • 제28권1호
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    • pp.150-165
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    • 2002
  • The purpose of this study was to evaluate bacteriocin activity against human flora. Lactobiocin, a bacteriocin produced by Lactococcus sp. HY 449, inhibited the growth of Starphylococcus epidermidis, Starphylococcus aureus, Streptoccoccus pyogenes and Propionibacterium acnes. When crude bacteriocin was added to indicator cells during logarithmic growth, the optical density(O.D 650nm) of cells without bacteriocin increase after 5h of incubation. Whereas in the presence of bacteriocin, the O.D of cell suspensions decreased. The similar patterns were observed for absorbance readings at 280 nm and 260 nm. The release of cellular components when cell were treated with Lactobiocin suggests some degree of membrane damage or cell lysis. Scanning electron microscopy of cells following treatments with Lactobiocin in PBS buffer revealed disruptures of cell morphology. These results indicate that bacteriocin appears to cause cell lysis of tested strains. In cytotoxicity on human fibroblast, LD$\_$50/ of Lactobiocin was ca. 50 mg/ml and no change was observed cell proliferation at the same concentration. Any irritation and allergic reaction did not observed when evaluated by human patch test for Lactobiocin.