• BMB Reports
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• v.31 no.6
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• pp.595-599
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• 1998

To investigate conformational information of a low oxygen affinity recombinant hemoglobin (rHb) containing $96Val{\rightarrow}Trp$ mutation at the ${\alpha}96$ position, we ave produced rHb (${\alpha}96Val{\rightarrow}Phe$) and rHb (${\alpha}96Val{\rightarrow}Tyr$), using the Escherichia coli expression system and site-directed mutagenesis. The oxygen affinity of rHb (${\alpha}96Val{\rightarrow}Phe$) is similar to that of human normal adult hemoglobin (Hb A). However, the oxygen affinity of rHb (${\alpha}96Val{\rightarrow}Tyr$) showed much lower oxygen affinity than Hb A which is similar to that of rHb (${\alpha}96Val{\rightarrow}Tyr$), providing an opportunity as a potential candidate for a hemoglobin-based blood substitute. Both rHb (${\alpha}96Val{\rightarrow}Phe$) and rHb (${\alpha}96Val{\rightarrow}Tyr)$ showed high cooperativity in oxygen binding. IH-NMR spectroscopy shows that both rHb (${\alpha}96Val{\rightarrow}Phe$) and rHb (${\alpha}96Val{\rightarrow}Tyr$) have very similar tertiary structure around the heme pockets and uaternary structure in the ${\alpha}_1/{\beta}_2$ subunit interface ompared to Hb A. The low oxygen affinity of rHb (${\alpha}96Val{\rightarrow}Tyr$) has been suggested to be due to a hydrogen bond caused by an extra hydroxyl group not present in rHb (${\alpha}96Val{\rightarrow}Phe$). However, investigation of the carbonmonoxy form of rHb (${\alpha}96Val{\rightarrow}Phe$) and (${\alpha}96Val{\rightarrow}Try$) in the presence of inositol hexaphosphate at low temperature suggests that low oxygen affinity of (${\alpha}96Val{\rightarrow}Try$) may arise from a mechanism different to that of rHb (${\alpha}96Val{\rightarrow}Trp$).

• Proceedings of the Microbiological Society of Korea Conference
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• 2007.05a
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• pp.120-122
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• 2007

All living organisms use numerous signal-transduction pathways to sense and respond to their environments and thereby survive and proliferate in a range of biological niches. Molecular dissection of these signalling networks has increased our understanding of these communication processes and provides a platform for therapeutic intervention when these pathways malfunction in disease states, including infection. Owing to the expanding availability of sequenced genomes, a wealth of genetic and molecular tools and the conservation of signalling networks, members of the fungal kingdom serve as excellent model systems for more complex, multicellular organisms. Here, we employed Cryptococcus neoformans as a model system to understand how fungal-signalling circuits operate at the molecular level to sense and respond to a plethora of environmental stresses, including osmoticshock, UV, high temperature, oxidative stress and toxic drugs/metabolites. The stress-activated p38/Hog1 MAPK pathway is structurally conserved in many organisms as diverse as yeast and mammals, but its regulation is uniquely specialized in a majority of clinical Cryptococcus neoformans serotype A and D strains to control differentiation and virulence factor regulation. C. neoformans Hog1 MAPK is controlled by Pbs2 MAPK kinase (MAPKK). The Pbs2-Hog1 MAPK cascade is controlled by the fungal "two-component" system that is composed of a response regulator, Ssk1, and multiple sensor kinases, including two-component.like (Tco) 1 and Tco2. Tco1 and Tco2 play shared and distinct roles in stress responses and drug sensitivity through the Hog1 MAPK system. Furthermore, each sensor kinase mediates unique cellular functions for virulence and morphological differentiation. We also identified and characterized the Ssk2 MAPKKK upstream of the MAPKK Pbs2 and the MAPK Hog1 in C. neoformans. The SSK2 gene was identified as a potential component responsible for differential Hog1 regulation between the serotype D sibling f1 strains B3501 and B3502 through comparative analysis of their meiotic map with the meiotic segregation of Hog1-dependent sensitivity to the fungicide fludioxonil. Ssk2 is the only polymorphic component in the Hog1 MAPK module, including two coding sequence changes between the SSK2 alleles in B3501 and B3502 strains. To further support this finding, the SSK2 allele exchange completely swapped Hog1-related phenotypes between B3501 and B3502 strains. In the serotype A strain H99, disruption of the SSK2 gene dramatically enhanced capsule biosynthesis and mating efficiency, similar to pbs2 and hog1 mutations. Furthermore, ssk2, pbs2, and hog1 mutants are all hypersensitive to a variety of stresses and completely resistant to fludioxonil. Taken together, these findings indicate that Ssk2 is the critical interface protein connecting the two-component system and the Pbs2-Hog1 pathway in C. neoformans.

• Korean Journal of Cognitive Science
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• v.21 no.4
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• pp.447-458
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• 2010

Time expressions are a very important form of information in different types of data. Thus, the recognition of a time expression is an important factor in the field of information extraction. However, most previously designed systems consider only a specific domain, because time expressions do not have a regular form and frequently include different ellipsis phenomena. We present a two-level recognition method consisting of extraction and transformation phases to achieve generality and portability. In the extraction phase, time expressions are extracted by atomic time units for extensibility. Then, in the transformation phase, omitted information is restored using basis time and prior knowledge. Finally, every complete atomic time unit is transformed into a normalized form. The proposed system can be used as a general-purpose system, because it has a language- and domain-independent architecture. In addition, this system performs robustly in noisy data like SMS data, which include various errors. For SMS data, the accuracies of time-expression extraction and time-expression normalization by using the proposed system are 93.8% and 93.2%, respectively. On the basis of these experimental results, we conclude that the proposed system shows high performance in noisy data.

• Proceedings of the KACD Conference
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• 2001.11a
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• pp.577-577
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• 2001

The lastest concepts in bonding are "total etch", in which both enamel and dentin are etched with an acid to remove the smear layers, and "wet dentin" in which the dentin is not blown dry but left moist before application of the bonding primer. Ideally, the application of a bonding agent to tooth structure should be insensitive to minor contamination from oral fluids. Clinically contaminations such as saliva, gingival fluid, blood and handpiece lubricant are often encountered by dentists during preparation of a restoration. The aim of this study was to evaluate the effect of contamination by hem-ostatic agents on shear bond strength of compomer restorations. One hundred and ten extracted human maxillary and mandibular molar teeth were collected. The teeth were cleaned from soft tissue remnant and debris and stored in physiologic solution until they were used. Small flat area on dentin of the buccal surface were wet ground serially with 400, 800 and 1200 abrasive paper on automatic polishing machine. The teeth were randomly divided into 11 groups. Each group was conditioned as follows: Group 1 : Dentin surface was not etched and not contaminated by hemostatic agents. Group2 : Dentin surface was not etched but was contaminated by Astringedent (Ultradent product Inc., Utah, U.S.A.). Group3 : Dentin surface was not etched but was contaminated by Bosmin (Jeil Phann, Korea.). Group4 : Dentin surface was not etched but was contaminated by Epri-dent (Epr Industries, NJ, U.S.A.). Group5: Dentin surface was etched and not contaminated by hemostatic agents. Group 6 : Dentin surface was etched and contaminated by Astringedent. Group7 : Dentin surface was etched and contaminated by Bosmin. Group8 : Dentin surface was etched and contaminated by Epri-dent. Group9 : Dentin surface was contaminated by Astringedent. The contaminated surface was rinsed by water and dried by compressed air. Group10 : Dentin surface was contaminated by Bosmin. The contaminated surface was rinsed by water aud dried by compresfed air. Group 11 : Dentin surface was contaminated by Epri-dent. The contaminated surface was rinsed by water and dried by compresfed air. After surface conditioning, F2000 was applicated on the conditoned dentin surface. The teeth were thermocycled in distilled water at $5^{\circ}C\;and\;55^{\circ}C$ for 1000 cycles. The samples were placed on the binder with the bonded compomer-dentin interface parallel to the lmife-edge shearing rod of the Universal testing machine(Zwick 020, Germany) running at a cross head speed of 1.0mmimin. There were no significant differences in shear bond strength between groups 1 and group 3 and 4, but group 2 showed significant decrease in shear bond strength compared with group 1. There were no significant differences in shear bond strength between group 5 and group 7 and 8, but group 6 showed significant decrease in shear bond strength compared with group 5. There were no significant differences in shear bond strength between group 5 and group 9, 10 and 11.

• Journal of the Korean Society for Library and Information Science
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• v.25
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• pp.83-150
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• 1993

Traditionally, the emphasis of the information services had been placed on the information provider's knowledge of information sources or information systems. However, in view of the increasing complexity of the information environment. and changes taking place in information professions the evaluation of the information services has callded for further research in interpersonal communication aspects of the librarian user interface especially that which links to user satisfaction. This experimental study was carried out for the purpose of exploring the implications of nonverbal behaviors in measuring user satisfaction in the light of the fact that the meaning of nonverbal clues includes more than verbal communication especially in dealing with variable barriers even though they operate at low levels of awareness. In this sense, among the wide range of nonverbal communication areas in particular kinesics, artifacts and the proximics were examined to suggest alternative model which can be applied effectively in our specific set of information work environment. Two major university libraries and two research libraries in Korea were chosen and visited for the anlysis and per each refernce/information interview the librarian's nonverbal behaviors were checked on the observer's checklist and the qustionnaires completed by the user at a time. On the basis of these data firstly, the relation of user satisfaction concerned with the librarian's nonverbal communication to user satisfaction with information services was analyzed and the possible communication barriers were investigated. In addition, the effect of training the information librarian with positive nonverbal communication skills on the user's satisfaction was analyzed in a SAS computer program in the area of kinesics. On the other hand, the effect of other nonverbal behaviors such as proximics and artifacts known very important clues to improving a complex human interaction was discussed along with their experimental results. In conclusion, it was found that most of users were not sensitive to the importance of nonverbal behaviors exposed by the information librarian, but it was proved that the ultimate user satisfaction and the satisfaction of the librarian's nonverbal behaviors are correlated positively, Moreover, nonverbal behaviors displayed by the trained librarian toward the user had a considerable effect on the user's evalution of information services compared with those by the untrained. These findings imply that in order to control the librarian's nonverbal behaviors and to help the user overcome his barriers the information librarian as a specialist needs to assure and modify willilingly his communication behavior. Without making effective use of such nonverbal communication, information librarian could not expect to meet the user's information needs either. However, when these positive nonverbal behaviors are applied it would be highly desirable to be aware of cultural and contextual differences. With regard to increasing information services success the applicable nonverbal communication model for library management and education can be suggested as below.

• Journal of KIISE:Computing Practices and Letters
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• v.9 no.6
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• pp.699-711
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• 2003

Home networking and Internet control technologies must satisfy three requirements with a view to being applied to home for raising the qualify of human life. Firstly, the cost of adding home networking ability to the traditional appliance must be minimized. Secondly, the mutual accessibility between home network and Internet must be guaranteed in the various Internet service environment of home. Finally, communication must be possible between the home network and the Internet that use different communications protocols. This paper proposes the ARCS (Appliance Remote Control System) that controls appliances based on LnCP (Living network Control Protocol) via the Internet. LnCP is the most cost-effective network and control protocol for home network. ARCS guarantees the accessibility from the Internet to home network and provides users with the virtual interface designed for controlling appliances using the Internet application program. In addition, this paper proposes AICS (Appliance Internet Control Specification). AICS, is based on LnCP, defines the message structure and control commands for the Internet control communication. ARCS and home gateway are developed to meet AICS.

• Journal of KIISE:Software and Applications
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• v.32 no.12
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• pp.1271-1282
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• 2005

Recently, PC-based control is incredibly developed in the industrial control field, but it is difficult for PLC programming in PC. Therefore, I need to develop the softeware PLC, which support the international PLC programming standard(IECl131-3) and can be applied to diverse control system by using C language. In this paper, I have developed the ISPLC(Intelligent Agent System based Software Programmable Logic Controller). In ISPLC system, LD programmed by a user which is used over $90\%$ among the 5 PLC languages, is converted to IL, which is one of intermediate codes, and IL is converted to the standard C rode which can be used in a commercial editor such as Visual C++. In ISPLC, the detection of logical error in high level programming(C) is more eaier than PLC programming itself The study of code conversion of LD->IL->C is firstly tried in the world as well as KOREA. I developed an execution engine with a good practical application. To show the effectiveness of the developed system, 1 applied it to a practical case, a real time traffic control(RT-TC) system. ISPLC is minimized the error debugging and programming time owing to be supported by windows application program.

• Science of Emotion and Sensibility
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• v.12 no.1
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• pp.77-86
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• 2009

A comfort evaluation of car seat clothing has been proposed for high comforts interior seat clothing. Car seat covers have received wide spread attention due to their man-machine interface working. And then, it will be necessary for measurements on delicate basic mechanical-properties, which closely relate with human touch feeling of its materials. In this research, we have utilized $KES-FB^{(R)}$(Kawabata Evaluation System) series, $^ST300{(R)}$ analogue softness tester and friction tester for measurement a physical properties. In order to consider both kansei and physical properties on interior seat covers, we firstly have established subjective words of judgement for the seat covers. Secondly, related them to the objective measurement of physical properties. Each kansei-language has clearly defined as 'Softness', 'Elasticity', 'Volume' and 'Stickiness' for the adjectives of leather car seat covers. These technical terms have correlated to physical properties in other words, h (mm), bending moment ($gf^*$cm/cm), To-Tm (mm) and ${\mu}$. At this time, fuzzy logic has utilized to predict the value of kansei language through physical values. On the basis of this result, finally it is possible to predict quality index of car seat covers using neural networks technique. In short, we develop a quality evaluation system of car seat clothing combining four physical quantities with kansei engineering.

• Restorative Dentistry and Endodontics
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• v.30 no.3
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• pp.204-214
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• 2005

This study compared the microtensile bond strength (${\mu}$TBS) and microscopic change of two 2-step and two 1-step self-etching adhesives to dentin according to storage times in distilled water. Occlusal dentin was exposed in 48 human molars. They were divided to four groups by different adhesives: SE Bond group (Clearfil SE Bond), AdheSE group (AdheSE). Adper group (Adper Prompt L-Pop), and Xeno group (Xeno III) . Each group was stored in 37$^{\circ}C$ distilled water for 1, 15, and 30 days. Resin-bonded specimens were sectioned into beams and subjected to ${\mu}$TBS testing with a crosshead speed of 1 mm/minute. For SEM observation, one specimen was selected and sectioned in each group after each stroage time. Resin-dentin interface was observed under FE-SEM. In all storage times, mean ${\mu}$TBS of SE group was significantly higher than those of other groups (p < 0.05). There was no significant difference between mean ${\mu}$TBS of SE group and AdheSE group among all storage times, but significant difference between 1- and 30-day storage in mean y${\mu}$TBS of Adper group and Xeno group (p > 0.05). For 1-and 15-day storage, all groups showed the close adaptation between resin-dentin interfaces. For 30-day storage, resin-dentin interfaces showed wide gap in Adper group and separate pattern in Xeno III group.

• The Journal of Society for e-Business Studies
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• v.13 no.2
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• pp.101-115
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• 2008

Recently in the human society, the crime using small arms has increased. Also, many accidents happen because of incomplete management of chemicals and radiation. Accordingly, it is necessary to manage dangerous objects by tracing the position of dangerous objects and rapidly providing the correct information for them. This paper presents a web-based system for real-time monitoring of dangerous objects using RFID in order to overcome the limitations and problems of current dangerous objects management techniques. In this paper, we define the architecture for web-based dangerous objects monitoring system and the scheme for storing information of a dangerous object in the RFID tag. We also implement the web-based monitoring system and present the execution result of the system. The proposed real-time monitoring system is composed of the dangerous objects monitoring server which manages information of dangerous objects and controls them, the dangerous objects monitoring middleware which is mediator between dangerous objects and the server, the RFID reader which reads information of dangerous objects from RFID tags attached to the objects and the database which stores information, status and position of dangerous object. The proposed system manages diverse dangerous objects such as small arms, radiation and harmful chemicals based on the position of them using RFID, so the user can check dangerous objects when they are checked in and checked out and the user can acquire the real-time position information of them through the system. Furthermore, the user can visually monitor dangerous objects through web browser from any where and at any time because the system is web-based system and it provides graphical user interface.