• Clinical and Experimental Reproductive Medicine
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• 제12권2호
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• pp.71-79
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• 1985

Systemic extrogen therapy promotes multiple preantral follicular development in immature mice. Estrogen pretreated ovaries might therefore be a useful source of cells for in vitro studies of oocytes maturation. Silastic capsules (5.0 mm length; 3.18 mm outer diameter, 1.57 mm inner diameter) filled with diethylstilbesterol were implanted subcutaneously in experimental mice (ICR) for up to 6 days. Ovarian weight and histology in diethylstilbesterol pretreated and control animal were assessed before and after pregnant mare serum gonadotrophin treatment and after human chorionic gonadotrophin. The following results were obtained; 1. Ovarian weight was significantly increased by 6 days of diethylstilbesterol pretreatment. Subsequent ovarian weight gain in response to pregnant mare serum gonadotrophin and human chorionic gonadotrophin was increased. 2. Diethylstilnbesterol pretreatment stimulated the developed healthy preantral follicles. 3. Forty eight hours after pregnant mare serum gonadotrophin treatment, a larger number of the antral follicles which developed in diethylstilbesterol pretreated animals showed signs of atresia, whereas in the control ovaries there was a higher incidence of premature luteinization. 4. Forty eight hours after human chorionic gonadotrophin, numerous corpora lutea and occasional luteinized unruptured follicles were present in both control and diethylstilbesterol ovaries. 5. Ovulation rate, fertilization rate and subsequent preimplantation development in vitro were not adversely affected by diethylstilbesterol pretreatment. However, there was considerable variation in the ovulation rate the number of animals with more than 60 ovulations was greater in the diethylstilbesterol gorup (52.4%) as compared to the control (33.3%).

• 대한생식의학회:학술대회논문집
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• 대한불임학회 2003년도 제45차 추계학술대회
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• pp.126.1-126.1
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• 2003

• 한국수정란이식학회지
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• 제33권2호
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• pp.69-73
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• 2018

This study was conducted to examine the influences of two human chorion gonadotrophins (hCGs) being injected into young or aged (45- to 65-week old) outbred (ICR) mice on developmental capacity of oocytes retrieved. In vitro-culture and parthenogenetic activation of oocytes retrieved were employed for the assessment. Superovulation was determined as being induced when more than 25 oocytes were retrieved. No aged mice were superovulated, while in contrast, 67-100% were superovulated in the 6- to 8-week-old (young) mice. In the aged, hCG injection yielded better retrieval (5 vs. 13 to 14.8 oocytes/mouse). Overall, no significant difference between two hCGs was detected but between the young and aged, significant differences in maturational arrest (0% vs. 39% MI arrest and 46% vs. 15% degeneration) and developmental capacity (24% vs. 46% 8-cell embryo development) were detected. In conclusion, hCG injection contributes to increasing oocyte retrieval from aged outbred mice, but the kinds of gonadotrophin influenced the efficiency of hyperstimulation induction in specific ages.

• 한국수정란이식학회지
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• 제33권3호
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• pp.149-157
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• 2018

The objective of this study was to evaluate the effect of treating dairy cattle with exogenous human chorionic gonadotrophin (hCG), five (5) days post artificial insemination (AI) on serum progesterone (P4) concentration and pregnancy rate. In this experiment, five days after AI, cows were assigned randomly to two groups namely: a) treated group (67) which were administrered with 1500 IU hCG (Chorulon) and b) control group (61), which received no treatment. On day 5, 10, 15 and 20 after the artificial insemination, blood samples from a total of 8 cows (4 from each group) were collected and were analyzed for serum P4 concentration. Cows were detected for estrus according to standing heat by visual observation. Cows that were detected still in estrus after days 18-24 were re-inseminated and recorded as not pregnant (open). Pregnancy diagnosis was conducted by ultrasonographic examination and transrectal palpation of the uterus on approximately 60 days in cows that observed to be not in estrus. The conception rate in hCG treated and control groups were 52.5 and 36.1%, respectively. The results proved that there were no significant differences in conception rate between two groups (p=0.0568). However, pregnancy rates were reduced by hCG treatment. Average serum P4 concentrations did not differ between Hcg-treated and control groups on day 5 (0.377 versus 0.375 ng/ml). On day 20 serum P4 concentrations were greater in the treated group compared with the control group (3.085 versus 2.010 ng/ml). The treatment with hCG seemed to increase P4 level compared with the control. In conclusion, the results of this study showed that 1500 IU of hCG administered on 5 day post AI increased conception rate in dairy cows. This was supported by the results on serum P4 concentration which was greater in hCG treated group.

• 한국임상수의학회지
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• 제3권1호
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• pp.227-233
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• 1986

A total of 600 Holstein cows in Chonnam province was examined to make a diagnosis on the ovarian follicular cyst. By clinical signs and rectal examination, 57 cows were found to have ovarian follicular cyst. Attempts were made to treat the cows which had ovarian follicular cyst with GnRH, HCG respectively. The results obtained were summarized as follows : 1. The rates of estreous induction with GnRH or HCG were 91.4%, 77.2%, respectively. The GnRH treated group was showed significantly higher than HCG treated group. The mean days from the GnRH or HCG treated to estrum were 25.1 and 23.5 days, respectively. 2. The Conception rates with GnRH or HCG treatment were 78.2% and 76.5％, respectively. 3. Services per conception with GnRH or HCG treatment were 1.5 and 2.1 respectively. 4, Days from GnRH or HCG treatment to concept were 38.2 and 45.8 days, respectively. 5. Intramuscular injection with GnRH and intraovarian injection with HCG were revealed the most effective routes in all the other routes.

• 한국수정란이식학회지
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• 제26권2호
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• pp.97-104
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• 2011

This study was carried out to confirm the effects of luteotrophin, human chorionic gonadotrophin (hCG), and an anti-luteolytic agent, flunixin meglumin (FM), on pregnancy rates in Hanwoo with in vitro produced (IVP) embryo transfers (ET), and to research the effects on the estrus cycle. Treatments included hCG and FM administration 3~10 minutes prior to ET. Also, pregnancy rates were compared with lidocane treatment and FM treatment prior to ET. The results are shown below. 30-day pregnancy rate was 76.7% in the hCG-treated group and 75.7% in the FM-treated group. Both rates were higher than the 70% rate for the control group. 42-day pregnancy rate was 76.7% in the FM-treated group. This was higher than 66.7% recorded for both the hCG-treated and control groups. The pregnancy rate of the hCG-treated group was high at Day 30 (76.7%) but low at Day 40 (66.7%), and there were no differences from the FM-treated and control groups. The recurrent estrus rate of infertile individuals at 2 weeks after ET was 36.4% in the hCG-treated group, under 71.4% in the FM-treated group and 80.0% in the control group. The non-pregnancy rate of individuals without recurrent estrus was 18.2% in the hCG-treated group, which was higher than the 0% rate in both the FM-treated and control groups. The pregnancy rates were higher in the FM-treated group than the Lidocane-treated group with 72.3% versus 67.5% in the heifers and 48.9% versus 43.6% in the cows. From the above results, the FM treatment proved more effective than the hCG treatment and no treatment whatsoever in increasing pregnancy rates after ET. In addition, hCG treatment was shown to be undesirable due to the deviations it caused in the reproductive physiology of the hCG-treated recipients. Therefore, in our study, the FM treatment resulted in a higher pregnancy rate than either lidocaine treatment or no-treatment in the trials of ET.

• 한국동물학회지
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• 제26권4호
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• pp.225-233
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• 1983

哺乳動物의 排卵時 濾胞卵子의 成熟再開와 더불어 卵子를 緻密하게 둘러싸고 있는 卵子細胞들의 分散이 일어난다. 이 現象은 生殖巢刺戟호르몬의 促進을 받은 卵丘細胞들이 細胞間隔에 多量의 뮤코量을 分泌함으로써 이루어지는데 이 때 cAMP가 第二 傳達者로 作用을 한다고 알려져 있다. 본 實驗에서는 卵子-卵丘 複合體를 培養하면서 HCG (10 IU/ml)에 의해 誘導된 卵丘細胞의 分散에 puromycin과 actinomycin D가 미치는 영향을 調査한 바 다음과 같은 缺課를 얻었다. 1. Puromycin은 2 $\\mu$g/ml의 濃度에서 卵丘細胞의 分散을 현저히 抑制하였으며 이 效果는 可逆的이었다. 2. Puromycin의 分散抑制效果는 HCG의 刺戟기간 (3시간) 뿐 아니라 뮤코量의 合成時期 ($3\\sim18$시간)에서도 나타났다. 3. Actinomycin D는 0.025 $\\mu$g/ml의 濃度에서부터 卵丘細胞의 分散을 抑制하기 시작했다. 4. Actinomycin D의 分散抑制效果는 부분적인 可逆性을 나타내었으며 0.1 $\\mu$g/ml의 濃度에서는 非可逆的인 災害效果를 나타내었다. 위의 缺課로부터 HCG의 卵丘細胞 分散誘導過程에는 蛋白質 내지 RNA의 合成過程이 관여하는 것으로 測定되며 따라서 cAMP는 轉寫 내지 解讀水準에서 卵丘細胞의 分散을 調節하는 것 같다.

• 한국수정란이식학회지
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• 제22권1호
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• pp.39-45
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• 2007

무발정기에 속한 Shih-tzu 견 9두를 대상으로 하여 PMSG(50IU/kg)를 10일 동안 매일 근육주사 후 마지막 10일째에 hCG(1,000IU/head)를 정맥 주사하여 인공적으로 발정을 유기 하였다. 실험견 9두 중 9두(100%)에서 발정 출혈, 회음부 반사, 외음부 종대 및 수컷 허용 등의 임상적인 발정 증상이 관찰되었으며 그중 5두(55.6%)가 임신하였고 4두는 임신되지 않았다(44.4%). 임신견 5두 중 3두(33.3%)가 자연분만하였는데 분만시 산자수는 $1.66{\pm}1.15$두 $(1{\sim}3)$이었고, 나머지 2두(40.2%) 는 PMSG 첫 투여후 38일과 41일에 조기태아사 하였다. 따라서 Shih-tzu 견에서 PMSG와 hCG 병용 투여에 의한 인공 발정 유기 효과는 발정 발현율 100%, 임신률 55.6%, 분만율이 33.3%이었다.

• 한국양식학회지
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• 제9권1호
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• pp.19-23
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• 1996

Hormonal induction of sex reversal was examined by using sex steroid hormones in serranid fish, Epinephelus septemfasciatus. Young fish were collected from the coastal area of Cheju Island, and reared for 2 years before fish were used for the experiments. Without any hormonal treatment, gonads of fish ($1,000\~2,800$ g in body weight) were occupied by oocytes of the perinucleolus stage and bundles of protogonial cells in the area of germinal epithelium. When the induction of sex reversal was attempted by daily oral administration of $17\alpha$-methyltestosterone (0.5 mg/kg fish) for 90 days, active spermatogenesis was induced, and spermatogonia and spermatocytes and spermatids were appeared in all gonads we examined. However, after daily, oral treatment of $17\beta$-estradiol (0.5 mg/kg fish) to. 50 days with the following injection of human chorionic gonadotrophin ($1,000\~1,500$ IU/kg fish) mature oocytes were not induced in fish gonad.

• 한국가축번식학회지
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• 제18권3호
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• pp.183-189
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• 1994

We examined early development in loach(Misgurnus mizolepis) embryos with parthenogenetic agents well-known in mammals. Female loach was superovulated with an intraperitoneal injection of 15 IU human chorionic gonadotrophin (hCG) per gram body weight. After 13 h of hCG injection, the oocytes were obtained from the abdomen. The oocytes were activated with 10% ethanol in tap water or fish Ringer's solution for 5, 10 and 15 minutes(eTW5, 10, 15 and eFRS5, 10, 15), respectively. The activation rates were 29% and 10% in eFRS10 and eFRS15, 5% and 6% in eTW10 and eTW15 by judging the cleaved blastomeres. Whereas, no parthenogenetic embryo was produced by tap water or fish Ringer's solution alone. The activation rate with the fish Ringer's solution was higher than that of tap water. No embryonic development was observed by calcium ionophore, A23187, at concentrations of 10, 20, 40 and 100$\mu$M when treated for 1, 2.5 and 5 minutes, respectively. The activation agents did not cause early development as in mammalian eggs. Therefore, the results suggest that fresh water fish may have a different egg activation pathway from that of mammals.