Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of Human Chorionic Gonadotrophin, Flunixin Meglumin, Lidocane on Pregnancy Rate with Hanwoo IVF Embryo Transfer

  • Yoon, Do-Joong (Department of Animal Science, Chungbuk National University) ;
  • Kim, Gye-Woong (Department of Animal Resources Community, KongJu National University) ;
  • Kim, Kon-Joong (Department of Animal Resources Community, KongJu National University) ;
  • Han, James-Bond (Department of Animal Science, Chungbuk National University) ;
  • Kim, Nam-Hyung (Department of Animal Science, Chungbuk National University) ;
  • Lee, Jong-Wan (Department of Animal Resources Community, KongJu National University)
  • Received : 2011.04.16
  • Accepted : 2011.04.27
  • Published : 2011.06.30
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Abstract

This study was carried out to confirm the effects of luteotrophin, human chorionic gonadotrophin (hCG), and an anti-luteolytic agent, flunixin meglumin (FM), on pregnancy rates in Hanwoo with in vitro produced (IVP) embryo transfers (ET), and to research the effects on the estrus cycle. Treatments included hCG and FM administration 3~10 minutes prior to ET. Also, pregnancy rates were compared with lidocane treatment and FM treatment prior to ET. The results are shown below. 30-day pregnancy rate was 76.7% in the hCG-treated group and 75.7% in the FM-treated group. Both rates were higher than the 70% rate for the control group. 42-day pregnancy rate was 76.7% in the FM-treated group. This was higher than 66.7% recorded for both the hCG-treated and control groups. The pregnancy rate of the hCG-treated group was high at Day 30 (76.7%) but low at Day 40 (66.7%), and there were no differences from the FM-treated and control groups. The recurrent estrus rate of infertile individuals at 2 weeks after ET was 36.4% in the hCG-treated group, under 71.4% in the FM-treated group and 80.0% in the control group. The non-pregnancy rate of individuals without recurrent estrus was 18.2% in the hCG-treated group, which was higher than the 0% rate in both the FM-treated and control groups. The pregnancy rates were higher in the FM-treated group than the Lidocane-treated group with 72.3% versus 67.5% in the heifers and 48.9% versus 43.6% in the cows. From the above results, the FM treatment proved more effective than the hCG treatment and no treatment whatsoever in increasing pregnancy rates after ET. In addition, hCG treatment was shown to be undesirable due to the deviations it caused in the reproductive physiology of the hCG-treated recipients. Therefore, in our study, the FM treatment resulted in a higher pregnancy rate than either lidocaine treatment or no-treatment in the trials of ET.

Keywords

References

  1. Bogacki M, Silvia WJ, Rekawiecki R and Kotwica J. 2002. Direct inhibitory effect of progesterone on oxytocin-induced secretion of prostaglandin $F_{2}$alpha from bovine endometrial tissue. Biol. Reprod. 67:184-188. https://doi.org/10.1095/biolreprod67.1.184
  2. Brackett BB and Oliphant G. 1975. Capacitation of rabbit spematozoa in vitro. Biol. Repod. 13:260-274.
  3. Butler WR, Calaman JJ and Beam SW. 1996. Plasma and milk urea nitrogen in relation to pregnancy rate in lactating dairy cattle. J. Anim. Sci. 74:858-865. https://doi.org/10.2527/1996.744858x
  4. Chagas e Silva J and Lopes da Costab L. 2005. Luteotrophic influence of early bovine embryos and the relationship between plasma progesterone concentrations and embryo survival. Theriogenology 64(1):49-60. https://doi.org/10.1016/j.theriogenology.2004.10.019
  5. Chagas e Silva J, Lopes da Costa L and Robalo Silva J. 2002. Plasma progesterone profiles and factors affecting embryo-fetal mortality following embryo transfer in dairy cattle. Theriogenology 58:51-59. https://doi.org/10.1016/S0093-691X(02)00906-8
  6. Diaz T, Schmitt EJ, de la Sota RL, Thatcher MJ and Thatcher WW. 1998. Human chorionic gonadotropin-induced alterations in ovarian follicular dynamics during the estrous cycle of heifers. J. Anim. Sci. 76:1929-1936. https://doi.org/10.2527/1998.7671929x
  7. Dunne LD, Diskin MG, Boland MP, O'Farrell KJ and Sreenan JM. 1999. The effect of changes in feed intake around the time of insemination on systemic progesterone and embryo development and survival in cattle. In: M.G. Diskin, Editor, Fertility in the High-Producing Dairy Cow. vol. II, no. 26, British Society of Animal Science Occasional Publication 381-384.
  8. Ferguson JKW. 1941. A study of the motility of intact uterus at term. Surg. Gynecol. Obste. 73:359-366.
  9. Garrett JE, Geisert RD, Zavy MT and Morgan GL. 1988. Evidence for maternal regulation of early conceptus growth and development in beef cattle. J. Reprod. Fertil. 84:437-446. https://doi.org/10.1530/jrf.0.0840437
  10. Hasler JF, Bowen RA, Nelson LD and Seidel GE. 1980. Serum progesterone concentrations in cows receiving embryo transfers. J. Reprod. Fertil. 58:71-77. https://doi.org/10.1530/jrf.0.0580071
  11. Helmer SD and Britt JH. 1986. Fertility of dairy cattle treated with human chorionic gonadotropin (hCG) to stimulate progesterone secretion. Theriogenology 5:683-695.
  12. Kastelic JP, Bergfelt DR and Ginther OJ. 1991. Ultrasonic detection of the conceptus and characterization of intrauterine fluid on days 10 to 22 in heifers. Theriogenology 35:569-581. https://doi.org/10.1016/0093-691X(91)90453-K
  13. Kenny DA, Boland MP, Diskin MG and Sreenan JM. 2001. Effect of pasture crude protein and fermentable energy supplementation on blood metabolite and progesterone concentrations and on embryo survival in heifers. Anim. Sci. 73:501-511. https://doi.org/10.1017/S1357729800058471
  14. Kerbler TL, Buhr MM, Jordan LT, Leslie KE and Walton JS. 1997. Relationship between maternal plasma progesterone concentration and interferon-tau synthesis by the conceptus in cattle. Theriogenology 47:703-714. https://doi.org/10.1016/S0093-691X(97)00028-9
  15. Lukaszewska J and Hansel W. 1980. Corpus luteum maintenance during early pregnancy in the cow. J. Reprod. Fertil. 59:485-493. https://doi.org/10.1530/jrf.0.0590485
  16. Mann GE and Lamming GE. 1995. Progesterone inhibition of the development of the luteolytic signal in cows. J. Reprod. Fertil. 104:1-5. https://doi.org/10.1530/jrf.0.1040001
  17. Mann GE, Lamming GE, Robinson RS and Wathes DC. 1999. The regulation of interferon-t production and uterine hormone receptors during early pregnancy. J. Reprod. Fertil. Suppl. 54:317-328.
  18. Nishigai M, Kamomae H, Tanaka T and Kaneda Y. 2002. Improvement of pregnancy rate in Japanese Black cows by administration of hCG to recipients of transferred frozen-thawed embryos. Theriogenology 58:1597-1606. https://doi.org/10.1016/S0093-691X(02)01062-2
  19. Nishigai M, Kamomae H, Tanaka T, Kaneda Y. 1997. Effect of xylazine tranquilization during embryo transfer to bovine recipients on pregnancy rates. J. Reprod. Dev. 43:41-46. https://doi.org/10.1262/jrd.97-436j41
  20. Nishigai M, Kamomae H, Tanaka T, Kaneda Y. 2001. The effect of human chorionic gonadotropin on the development and function of bovine corpus luteum. J. Reprod. Dev. 47:283-294. https://doi.org/10.1262/jrd.47.283
  21. Nishigai M. 2003. The development and prevalence of the transfer technique for frozen-thawed embryos of Japanese black beef cattle in tochigi prefecture. Journal of Reproduction and Development 49(1):23-36. https://doi.org/10.1262/jrd.49.23
  22. Price CA and Webb R. 1989. Ovarian response to hCG treatment during the oestrous cycle in heifers. J. Reprod. Fertil. 86:303-308. https://doi.org/10.1530/jrf.0.0860303
  23. Purcella SH, Beala WE, and Grayb KR. 2005. Effect of a CIDR insert and flunixin meglumine, administered at the time of embryo transfer, on pregnancy rate and resynchronization of estrus in beef cattle. Theriogenology 64(4):867-878. https://doi.org/10.1016/j.theriogenology.2004.12.015
  24. Santos JEP, Thatcher WW, Pool L and Overton MW. 2001. Effect of human chorionic gonadotropin on luteal function and reproductive performance of high-producing lactating Holstein dairy cows. J. Anim. Sci. 79:2881-2894. https://doi.org/10.2527/2001.79112881x
  25. Schmitt EJP, Barros CM, Fields PA, Fields MJ, Diaz T and Kluge JM et al.. 1996. A cellular and endocrine characterization of the original and induced corpus luteum after administration of a gonadotropin-releasing hormone agonist or human chorionic gonadotropin on day five of the estrous cycle. J. Anim. Sci. 74:1915-1929. https://doi.org/10.2527/1996.7481915x
  26. Schrick FN, Hockett ME, Towns TM, Saxton AM, Wert NE and Wehrman ME. 2001. Administration of a prostaglandin inhibitor immediately prior to embryo transfer improves pregnancy rates in cattle. Theriogenology 55:370.
  27. Schrick FN, Scenna FN, Edwards JL, Hockett ME and Saxton AM. 2003. More evidence for a direct interaction between prostaglandin $F_{2{\alpha}}$ and development of bovine embryos. Proceedings of the CETA and AETA Joint Annu. Conv. Calgary, Alberta 43-52.
  28. Seals RC, Lemaster JW, Hopkins FM and Schrick FN. 1998. Effects of elevated concentrations of prostaglandin $F_{2{\alpha}}$ on pregnancy rates in progestogen supplemented cattle. Prostaglandins 56:377-389. https://doi.org/10.1016/S0090-6980(98)00063-X
  29. Shemesh M, Milaguir F, Ayalon N and Hansel W. 1979. Steroidogenesis and prostaglandin synthesis by cultured bovine blastocysts. J. Reprod. Fertil. 56:181-185. https://doi.org/10.1530/jrf.0.0560181
  30. Sianangama PC and Rajamahendran R. 1992. Effect of human chorionic gonadotrophin administered at specific times following breeding on milk progesterone and pregnancy in cows. Theriogenology 38:85-96. https://doi.org/10.1016/0093-691X(92)90220-L
  31. Smith AK, Broadbent PJ, Folman DF, Grimmer SP, Davies DAR and Dobson H, Norgestomet implants. 1996. plasma progesterone concentrations and embryo transfer pregnancy rates in cattle. Vet. Rec. 139:187-191. https://doi.org/10.1136/vr.139.8.187
  32. Spell AR, Beal WE, Corah LR and Lamb GC. 2001. Evaluating recipient and embryo factors that affect pregnancy rates of embryo transfer in beef cattle. Theriogenology 56:287-297. https://doi.org/10.1016/S0093-691X(01)00563-5
  33. Sreenan JMBeehan D, Diskin MG. 1987. Factors affecting pregnancy rate following embryo transfer in the cow. Theriogenology 27:99-113. https://doi.org/10.1016/0093-691X(87)90073-2
  34. Starbuck GR, Darwash AO, Mann GE and Lamming GE. 1999. The detection and treatment of post insemination progesterone insufficiency in dairy cows. In: M.G. Diskin, Editor, Fertility in the High-Producing Dairy Cow vol. II, no. 26, British Society of Animal Science Occasional Publication 447-450.
  35. Stubbings RB and Walton JS. 1986. Relationship between plasma progesterone concentrations and pregnancy rates in cattle receiving either fresh or previously frozen embryos. Theriogenology 26:145-155. https://doi.org/10.1016/0093-691X(86)90019-1
  36. Tervit HR, Cooper MW, Goold PG, Haszard GM. 1980. Non-surgical embryo transfer in cattle. Theriogenology 13:63-71. https://doi.org/10.1016/0093-691X(80)90015-1
  37. Thatcher WW, Guzeloglu A, Mattos R, Binelli M, Hansen TR and Pru JK. 2001. Uterine-conceptus interactions and reproductive failure in cattle. Theriogenology 56:1435-1450. https://doi.org/10.1016/S0093-691X(01)00645-8
  38. Walton JS, Halbert GW, Robinson NA and Leslie KE. 1990. Effects of progesterone and human chorionic gonadotropin administration five days post insemination on plasma and milk concentrations of progesterone and pregnancy rates of normal and repeat breeder dairy cows. Can. J. Vet. Res. 54:305-308.
  39. Wann RA and Randel RD. 1990. Effect of uterine manipulation 35 days after parturition on plasma concentrations of 13,14-dihydro-15-keto prostaglandin $F_{2{\alpha}}$ in multiparous and primiparous Brahman cows. J. Anim. Sci. 68:1389-394. https://doi.org/10.2527/1990.6851389x
  40. Wathes DC, Robinson RS, Mann GE and Lamming GE. 1998. The establishment of early pregnancy in cows. Reprod. Domest. Anim. 33:279-284. https://doi.org/10.1111/j.1439-0531.1998.tb01358.x
  41. Wolf E, Arnold GJ, Bauersachs S, Beier HM, Blum H and Einspanier R et al. 2003. Embryo-maternal communication in bovine-strategies for deciphering a complex crosstalk. Reprod. Domest. Anim. 38:276-289. https://doi.org/10.1046/j.1439-0531.2003.00435.x