• 패션비즈니스
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• 제17권1호
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• pp.170-185
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• 2013

The suitability of the pattern manufactured with the development figure was considered by reviewing the development conditions that can be directly connected to the basic pattern in the human body surface development figure with the cast bandage method. The method to prepare the sleeve basic pattern was based on the cylindrical surface development method, and the sleeve basic pattern covering the 45 and 90 degrees momentum of the arm-movement was made by using the cast-type body surface development figure prepared with the horizontal line of the sleeve hem placed horizontally in the plan and by combining the cast-type body surface development figure in the standing position with the figure in the moving position. The test clothing was prepared with the sleeve pattern adding the bodice pattern in the standing position and the momentum and was worn on the FRP replica. The relationship theory of the body surface development figure with the pattern was derived by reviewing the suitability from the wearing state. The sleeve-cap height of the sleeve basic pattern resulted in about 80% in the standing position when the needs for a physical activity are 45 degrees and the about 50% when the needs for a physical activity are 90 degrees. The additional size of the diagonal length of the sleeve-cap could be set as "0" if the sleeve-cap height is low by 50% and as 50% of the additional size in the standing position if the sleeve-cap height is 80%.

• 인터넷정보학회논문지
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• 제11권1호
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• pp.129-141
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• 2010

본 논문에서는 사람의 행동 모니터링을 위한 멀티 센서 기반의 웨어러블 지능형 디바이스를 제안한다. 다중 행동을 인식하기 위해, 이미지 센서와 가속도 센서를 이용하여 행동 인식 알고리즘을 개발하였다. 멀티 센서로부터 얻은 데이터를 분석하기 위해 그리드 기반 옵티컬 플로우 방법을 제안하고 SVM 분류기법을 이용하였다. 이미지 센서로부터 얻은 모션 벡터의 방향과 크기를 이용하였고, 3축 가속도 센서로부터 얻은 데이터에서 FFT의 축과 크기와의 상관관계를 계산하였다. 실험 결과에서 이미지 센서 기반과 3축 가속도 센서기반의 행동 인식률은 각각 55.57 %, 89.97%를 보였으나 제안한 멀티센서기반의 행동인식률은 92.78% 를 보였다.

• Reproductive and Developmental Biology
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• 제29권4호
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• pp.259-267
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• 2005

The present study was conducted to investigate gelatinolytic activities in HAM and to determine whether there are any changes in gelatinolytic activity profiles when the cells are cultured in hepatogenic medium. Placenta was obtained during caesarean section of the volunteers, with informed consent. HAM were isolated from amniotic membrane using collagenase type A HAM were cultured in hepatogenic medium for 3 weeks and the conditioned media were obtained at day 7, 14 and 21. The zymographic pattern of gelatinolytic activity of the HAM did not undergo a change during passages. When the HAM were cultured in a fibronectin-coated dishes in a hepatogenic medium, there was no significant difference of the gelatinase pattern between before and after culture. However, when bFGF was added to the culture, a dramatic increase of 62kDa and 59kDa gelatinases was observed. Interestingly, when ITS instead of FN was present, HAM-conditioned medium also showed a similar increase of both gelatinases. Immunoblotting analysis demonstrated that both 62kDa and 59kDa gelatinases were the active form of MMP-2 resulting from the turnover of MMP-2 proform. Futher study will be necessary to determine the relationship between bFGF and active MMP-2 during hepatogenesis of HAM.

• 한국자원식물학회지
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• 제31권4호
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• pp.342-354
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• 2018

Suaeda japonica is a halophytic plant that lives in mudflat at intertidal zone of western and southern coastal areas of Korea. The seawater-living plants showed a purple color during their whole life. In contrast, freshwater-living plants displayed a green color in leaves. When seawater-living plants were transferred to potting soil, the purple color was gradually changed to green in the leaves. The extracted purple pigment compound exhibited typical characteristics of betacyanin that were represented by water solubility, pH- and temperature-dependent color changes, sensitivity to light, UV-Vis spectra, and gel electrophoretic migration pattern. The LC-MS analysis of the extracted pigment compound showed the presence of two major protonated molecular ions ($[M+H]^+$) at m/z 651.1 and m/z 827.1. Antioxidant activity of the pigment compound was determined using stable free radical DPPH assay. It was found to have an antioxidant activity that is linearly increased in proportion to the reaction time for up to 30 min, and the activity was comparable to that of control BHA at 9.0 mg/ml. The anticancer activity against several tumor cell lines was also examined following the MTT assay. The significant growth inhibitory effect was observed on two tumor cell lines, SW-156 (human kidney carcinoma) and HEC-1B (human endometrial adenocarcinoma). Probably, the pigment compound may function as an osmolyte to uphold halotolerant physiological processes in saline environment.

• 한국게임학회 논문지
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• 제11권1호
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• pp.121-129
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• 2011

온라인 게임에서 오토 프로그램 또는 봇 프로그램으로 인하여 다양한 게임 서비스 피해사례가 발생하고 있다. 특히, 게임 머니 및 아이템의 비정상적인 수집은 게임이 가지는 본연의 재미를 잃어버리게 되고, 궁극적으로 게임 생명주기에 결정적 악영향을 미치게 된다. 본 논문은 게임 봇 감지를 위해 게임 행위 변화 패턴을 수집하고 분석하여 봇 탐지 방법에 적용한다. 인간의 게임 행위 변화 정보와 봇의 게임 행위 변화 정보를 이용하여 유사정도를 측정하고, 봇 탐지 기법에 활용하는 것이다. 실험에서는 서비스 중인 온라인 게임을 이용하여 사용자와 봇의 모델을 생성하고 유사성을 판별하였으며 적절한 결과를 확인하였다.

• 한국산림과학회지
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• 제99권3호
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• pp.259-266
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• 2010

우리나라에서 산불 발생 공간분포는 인간 활동과 큰 관련성이 있기 때문에, 지역별 군집형태의 강한 공간의존성을 갖는다. 본 연구에서는 공간의존성의 개념에 입각하여 점자료 분석법을 통한 산불발생의 공간분포패턴을 분석하였다. Ripley의 K 함수를 이용하여 산불 발생 원인별 공간분포 형태를 파악하였으며, Kernel 함수를 통해 산불발생의 공간적 집중도를 분석하였다. 그 결과 정도는 상이하지만 모든 원인의 산불이 임의(random) 분포가 아닌 군집화(clustered)되어 발생하는 특징이 있는 것으로 나타났다. 또한, 산불 발생의 군집성을 원인별로 크게 두 집단으로 나눌 수 있었다. 첫째는 전국적 발생 패턴을 가지는 원인으로 입산자 실화, 논밭두렁 소각과 같은 활동과 관련된 것이고 또 다른 하나는 국지적 군집성을 가지는 원인으로 담뱃불이나 어린이 불장난, 방화이다. 그 군집성의 범위는 30 km내외로 나타났으며, 그 범위 밖에서는 임의 분포하고 있었다. Kernel 함수에 의한 원인별 집중도 분석에서는 강한 군집도를 나타냈던 3가지 원인(담뱃불, 어린이 불장난, 방화)의 경우 대부분 인구밀도가 높은 수도권을 중심으로 발생하는 것을 확인할 수 있었다.

• Environmental Analysis Health and Toxicology
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• 제18권1호
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• pp.15-19
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• 2003

Metallothionein gene expression activity of cadmium was investigated in a human lung epithelial cell line. Cells, grown to near confluence, were exposed to 0∼10 ${\mu}$M Cd metal for 6 hours. Cadmium did not cause morphological alteration in lung epithelial cells that are characteristic of cell damages such as cell shrinkage, detachment of the cell from its neighbors, cytoplasmic and chromatic condensation. However, metallothionein genes of MT-1 and MT-2 were rapidly induced in the treated cell measured by RT-PCR. Regarding the induction pattern of motallothionein mRNA, MT-1 mRNA was induced in a dependent manner. MT-2 mRNA induction, which was measured using oligo primers based on cDNA of human reticulocytes, seemed to be slightly increased in low doses but decreased at high concentration used in the experiment.

• 대한미생물학회지
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• 제22권1호
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• pp.23-33
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• 1987

To detect lepotospiral strains which produce hemolysin and determine the optimal condition for assaying hemolysin, we screened reference strains and observed some property of hemolysin. Hemolysin activity was assayed with cell free culture liquids and erythrocyte suspension. The production of hemolysin by local strains isolated in Korea was assayed and compared with that of reference strains. The hemolysin was produced by 18 strains among 38 reference strains and 3 local strains isolated in Korea. The production of hemolysin began with growth of Leptospira cultured in EMJH medium and reached maximum at stationary phase. The optimum temperature for hemolytic activity was $37^{\circ}C$. At lower temperature the activity of hemolysin was decreased progressively. The hemolytic activity was completely inactivated after :30 minutes' exposure at $56^{\circ}C$. Hemolysis pattern was "hot-cold type" which showed increased hemolysis after cold incubation. The hemolysin was most active on sheep erythrocyte and less active on ox, goat, human and guinea pig erythrocyte with the decreasing order.

• 한국환경보건학회지
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• 제34권2호
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• pp.137-147
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• 2008

This study evaluated the effects of the human activity and outdoor air on concentrations of size-selective particulate matters (PM) by conducting a realtime measurement in classrooms and on roofs at 4 elementary schools, 3 middle schools and 3 high schools in Incheon City. PM concentrations featured repetitive pattern of increasing during break time (including lunch hours) and cleaning time while decreasing during class hours. This trend was more prominent with inhalable PM and PM10 than fine PMs (PM2.5, PM1.0). The indoor/outdoor (I/O) ratio of inhalable PM and PM10 exceeded 1 while that of fine PMs was close to or below 1. The PM2.5 (out)/PM10 (out) ratio stood at 0.59 (${\pm}0.16$) and the PM2.5 (in)/PM10 (in) ratio was 0.29 (${\pm}0.09$), suggesting that occupant activity had a greater effect upon coarse particles (PM10-PM2.5) than upon fine particles (PM2.5, PM1.0). The correlations between the indoor and the outdoor PM concentrations showed a stronger positive correlation for fine particles than that of coarse particles. The linear regression analysis of PM concentrations indoor and outdoor indicated a higher determinant coefficient ($r^2>0.9$), and consistency for fine particles than in case of coarse particles. In conclusion, the results of this study suggest that the indoor coarse particle concentration is more attributed to occupant activity and the indoor fine particle concentration is more influenced by outdoor air pollution.

• Journal of Periodontal and Implant Science
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• 제32권1호
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• pp.235-247
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• 2002

The aim of this study was to evaluate the effects of chitosan coating on the attachment, proliferation, functional and morphological change of human gingival fibroblasts. Primary culture of human gingival fibroblasts were grown in Dulbecco's modified Eagle's medium with 10% fetal bovine serum and 1% antibiotics. In experimental group, cells were inoculated in the multiwell plates coated with chitosan in concentration of 0.02, 0.2, and 2 mg/ml. Cell counting and MTT assay were done after 0.5, 1.5, 3, 6 and 24 hours of incubation to evaluate the cell attachment, and then after 2 and 7 days of culture to evaluate the cell proliferation. The alkaline phosphatase activity was measured after 4 and 7 days of culture and the ability to produce mineralized nodules was evaluated after 21 days of culture. The results were as follows : The morphology of cells on the chitosan-coated well was round or spheric. Round cells were aggregated since 6 hours of culture and showed nodule-like appearance after 24 hours of culture and did not achieved confluency at 7 days. The attachment of gingival fibroblasts was inhibited by chitosan coating with a tendency of dose dependent pattern. But, cellular activity of unit cell was higher than control. The proliferation of gingival fibroblasts was inhibited by chitosan coating at 2 mg/ml(P<0.01), while the cell proliferation at 0.02, 0.2 $mg/m{\ell}$ was comparable to the control well. Total alkaline phosphatase activity was inhibited by chitosan coating and decreased in the course of time. While ALP activity of unit cell was the highest at 2mg/ml after 4 days of culture. Finally, gingival fibroblasts produced the mineralized nodule at 2 mg/ml. In summary, the attachment, proliferation, and alkaline phosphatase activity of gingival fibroblasts were influenced differently by the concentration of coated chitosan. From this study, it could be used as the matrix of tissue engineering for gingiva without inhibition on proliferation of gingival fibroblasts using chitosan at the optimal concentration (0.02mg/ml).