Objectives: This study was carried out to evaluate the effects of embryonic stage, cryoprotectant, and freezing-thawing method on the rates of survival and development of the cryopreserved mouse early embryo and finally to establish the cryopreservation method of surplus embryos obtained during assisted reproductive technology (ART). Materials and Methods: Two to eight cell embryos were obtained from oviducts of mated $F_1$ hybrid female mice superovulated by pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Two-step 1,2-propanediol (PROH), dimethylsulfoxide (DMSO) and 4-step PROH DMSO were used as cryoprotectant and dehydration and rehydration method of embryos, and slow-cooling or rapid-cooling method was used as frozen program. The survival rates of embryos were measured after thawing and rehydration, and the developmental rates of embryos were compared and observed during culturing embryos for 24, 48, 72, 96 hrs. Results: As for the survival and development rates of embryos according to embryonic stage, the survival rate of 2 cell stage in PROH and DMSO was significantly higher than 4-8 cell (64.5% versus 62.1 %,79.7% versus 73.2%) (p<0.01, p<0.01), but the development rates of 4-8 cell embryos in PROH and DMSO were significantly higher than 2 cell embryos for whole culture period (p<0.01) and the development rates of 4-8 cell embryos in PROH were significantly higher than 2 cell embryos in DMSO (p<0.01). As for the survival and development rates of embryos according to cryoprotectant, the survival rate of 2 cell embryo in DMSO was significantly higher than that in PROH (74.4% versus 64.5%) (p<0.01), whereas the development rate of embryos was not differ till 24 hrs. The developmen1 rate from morular to hatching blastocyst, however, was significantly higher in PROH than in DMSO during 48 hr (p<0.01). The survival rate of 4-8 cell embryo was 62.1% in PROH and 73.2% in DMSO. The development rates of embryo in PROH were significantly higher for whole culture periods (p<0.01, 0.05). In respect to the effect of freezing and thawing program on the survival and development rates of embryos, method of slow cooling and rapid thawing was more effective than that of rapid cooling and rapid thawing. Conclusions: The survival rate of embryo in 2 cell stage was higher than in 4-8 cell stage, and PROH appears more effective cryoprotectant than DMSO because PROH showed better development rates of embryos in 2 and 4-8 cell stage. Moreover, slow cooling and rapid thawing method was considered as the best cryopreservation program.
The purpose of this study is to investigate the structural relationship between organizational citizenship behavior and behavioral intention. The purpose of this study is to investigate the relationship between conflicts, organizational citizenship behaviors, and behavioral intentions in order to achieve research objectives. Based on this study, hypotheses were established and the implications and implications of these results were sought. First, the relationship conflicts of hotel employees have significant relationship to organizational citizenship behavior. Second, the relationship conflicts of hotel employees have a meaningful relation to the behavioral intention. Third, organizational citizenship behavior of hotel employees has a meaningful relationship with behavioral intention. As the results show, the company seeks for profit but it is also another way for employees to stay in one job and to make an opportunity to contribute value to the company. As the relationship between the individual conflicts, organizational citizenship behaviors, and behavioral intentions, how to solve the conflict problem is the task of the hotel company based on the human resources. Future research and limitations are needed for the efficiency of this study, but it is necessary to specify samples and specimens through an in-depth review of the specimens.
This researcher has sorted out the development process of death aesthetics contained in human art through advanced research. This research is a follow-up study of related research, which studies the aesthetic meaning of death in Alexander McQueen's fashion works. This study first classifies the aesthetic characteristics of death, and then analyzes and sorts out the aesthetics of death in Alexander McQueen's clothing works based on this. The results of this study are as follows: first, the aesthetic manifestations of death are divided into three categories: reproducibility, symbolic and religious. Among them, the representation of reproducibility includes the representation of real objects and the representation of virtual objects, and the symbolic performance includes two types of external performance and internal performance. Second, in Alexander McQueen's works, the reproducibility of death was mainly reflected by virtual stage scenes and costumes. Thirdly, the symbolic death is not only reflected in the skeletons and blood etc. directly related to the death of the body, but also in the various emotions and psychological states caused by the death. Fourth, the religious nature of death was represented in Alexander McQueen's works through such representative things as crosses and religious stories.
Journal of the Society of Cosmetic Scientists of Korea
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v.37
no.1
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pp.23-36
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2011
The purpose of this study is to make theoretical examinations into the makeup trend shown in digital communication environment and thereby determine potential associations of makeup trend with digital communication environment and characterize said trend, so that it can provide reference materials required for follow-up studies. This study comes to the following findings: First, it is found that there are sociocultural characteristics of digital communication environment, such as interactivity, technology-orientedness, sentiment-orientedness and mixture of time and space. Secondly, it is found that trends developed in makeup fall categorically into interactivity, virtuality, amusement and mobility. Thirdly, it is found that each type of digital communication adopts a mixture and juxtaposition of contemporary styles or categories with traditional ones through eclectic combination based on open-minded and horizontal ideas. Fourthly, it is found that a trend from digital communication environment doesn't coincide perfectly with any makeup trend, but the former trend works interactively and organically with the latter trend on the basis of homogeneity and similarity within a general framework that represents external esthetics of human being. With the creation of digital communication environment, our contemporary society forms as the complex phenomena of digital society in a combination and destruction of different categories beyond any certain international boundary, so that a hybrid tendency appears in contemporary makeup trend as well. Based on these findings, this study has its implications in the sense that it gives a new viewpoint based on design, formative characteristics and expression styles of makeup, and also provides useful reference materials for related industrial sectors.
In almost industries, such as the logistics industry, marine fisheries, agriculture, industry, and services, small unmanned aerial vehicles are used for aerial photographing or closing flight in areas where human access is difficult or CCTV is not installed. Also, based on the information of small unmanned aerial photographing, application research is actively carried out to efficiently perform surveillance, control, or management. In order to carry out tasks in a mission-based manner in which the set tasks are assigned and the tasks are automatically performed, the small unmanned aerial vehicles must not only fly steadily but also be able to charge the energy periodically, In addition, the unmanned aircraft need to land automatically and precisely at certain points after the end of the mission. In order to accomplish this, an automatic precision landing method that leads landing by continuously detecting and recognizing a marker located at a landing point from a video shot of a small UAV is required. In this paper, it is shown that accurate and stable automatic landing is possible even if simple template matching technique is applied without using various recognition methods that require high specification in using low cost general purpose small unmanned aerial vehicle. Through simulation and actual experiments, the results show that the proposed method will be made good use of industrial fields.
The aim of this paper is to project the state of the labor farce and employment in Korea from 2000 to 2005. The labor market in Korea is experiencing significant changes with the rapid development of Information and Telecommunication Technology (ICT) and the transition of the Korean economy into a knowledge-based economy. On the labor supply side, it is expected that the growth of the labor force will be sluggish; baby boomers will become the middle-aged, while the proportion of senior citizens, the highly educated and the female labor force will grow fast. These changes will alter the human resources management system in business sectors. Moreover, the permanent employment relationship, the hierarchy system and the seniority-based wage system are all expected to change. On the labor demand side, the employment share in highly skilled. knowledge-intensive industries will grow faster than the rest of the economy in tandem with the quickly growing output share of these industries. Especially, more jobs will be created in the ICT industries. The proportion of labor in highly skilled and professional occupations will also grow faster than in other occupations. At the same time, the employment share of female workers will grow more quickly than that of the male workers. These changes, however, may worsen income inequalities and/or increase the unemployment rate when workers do not have the suitable skills or knowledge required by the knowledge-based economy. To avoid this, it is necessary for the government to build up a lifetime learning system for workers.
Hong, Sung Wook;Bae, Hyo Ju;Chang, Jin Hee;Kim, So-Young;Choi, Eun-Young;Park, Beom Young;Chung, Kun Sub;Oh, Mi-Hwa
Journal of Dairy Science and Biotechnology
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v.31
no.2
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pp.153-159
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2013
Lactic acid bacteria are microorganisms that are closely associated with human and/or animal environments, and are categorized as generally recognized as safe (GRAS) organisms due to their ubiquitous appearance in foods and their contribution to the healthy microflora of mucosal surfaces. This study was performed to isolate and identify lactic acid bacteria with antagonistic effects against food-borne pathogens. A total of 3,000 acid-producing bacteria were isolated from infant feces, cattle feces, goat feces, dog feces, pig feces, vaginal tracts, vegetables, fruits, Kimchi, Jeotgal, fermented sausages, raw milk, cheese, yogurt, Cheonggukjang, Meju, and Makgeolli cultured on MRS agar with 0.05% bromocresol purple. For the isolation of bacteriocin-producing bacteria, the diameter of the clear zone was measured on MRS agar plates. Twenty-six isolates exhibited strong antibacterial activity against indicator strains such as Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella enterica serovar Enteritidis. Lactic acid bacteria were identified as Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Lactobacillus acidophilus, Lactobacillus amylovorus, Lactobacillus curvatus, Lactobacillus plantarum, and Pediococcus acidilactici by 16S rDNA gene sequence analysis. The results of this study suggest that the isolates could be used as potential probiotic starters for functional food applications.
Business Process Intelligence (BPI) is one of the emerging technologies in the knowledge discovery and analysis area. BPI deals with a series of techniques from discovering knowledge to analyzing the discovered knowledge in BPM-supported organizations. By means of the BPI technology, we are able to provide the full functionality of control, monitoring, prediction, and optimization of process-supported organizational knowledge. Particularly, we focus on the focal organizational knowledge, which is so-called the BPM activity-performer affiliation networking knowledge that represents the affiliated relationships between performers and activities in enacting a specific business process model. That is, in this paper we devise a statistical analysis method to be applied to the BPM activity-performer affiliation networking knowledge, and dubbed it the activity-performer correspondence analysis method. The devised method consists of a series of pipelined phases from the generation of a bipartite matrix to the visualization of the analysis result, and through the method we are eventually able to analyze the degree of correspondences between a group of performers and a group of activities involved in a business process model or a package of business process models. Conclusively, we strongly expect the effectiveness and efficiency of the human resources allotments, and the improvement of the correlational degree between business activities and performers, in planning and designing business process models and packages for the BPM-supported organization, through the activity-performer correspondence analysis method.
This study is to investigate the color emotion associations of the color vision defectives, considering that the colors do have an effect on human emotional conditions. To realize this investigation, firstly we selected 100 normal persons (group C)and other 34 color vision defectives(group A), dividing the last group into two small groups as protanomaly group(group P) with 8 persons and deuteranomaly group(group D) with 16 persons. All participants have been offered to select one color from ten colors for each of three positive emotions such as 'favorite', 'happy' and 'friendly' and of three negative emotions like 'sad', 'disliked' and 'awkward'. And they selected another one color for each active and passive emotions. For 'favorite color' the group C selected 'blue' and 'red' while the group A chose 'blue'. For 'happy color' the two groups selected 'yellow'. For 'friendly color' the group C chose 'green', but the group A selected 'blue'. For 'sad color' the group C preferred 'blue', but the group A chose 'purple'. For 'disliked color' all groups selected 'bluish green'. For 'awkward color' the two groups preferred 'bluish green'. For 'active color' all groups selected 'red'. And for 'passive color' the group C chose 'bluish green', but the group A selected 'blue'. Depending on the type of color vision deficiency(group P and group D) some more differences were revealed relatively. These results should be applied to develop some intelligent color conversion technology for enhancing the usability of culture contents for color vision defectives.
Pharmacopunture is a new combined method of acupunture and oriental drugs. Recently, this method is widely used to treat traffic accident patients in oriental medicine. However, there is no evidences of treatment, no information of effects and side-effects of this method, and no information of drugs used. In South Korea, western medicine and oriental medicine are regulated differently. When a new technology is invented in the area of western medicine, that method should pass several stages of clinical trials. After that processes, that method can be done as a medical practice. However, in the area of oriental medicine, there is no process like that. According to in South Korea, medical practice without license are composed of two behaviors. First type is that medical practice is done by a person who has no medical license. Second type is that medical practice is done by a person who has a medical license but the area of the license is different. Because of this reason, the distinction between the western medical practices and the oriental medical practices is very important. Medical practices are protected by license mainly because they can harm human life or body. When we invented new medical practice and try to practice it to the patients, we should consider the risk of that method whether it is western medical practice or oriental medical practice. It is not clear that the pharmcopunture which has been done is satisfied the standard of medical treatment.
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