• Journal of Life Science
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• v.31 no.11
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• pp.1028-1036
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• 2021

Firefly luciferase (FLuc) can function as an efficient marker in the gene and viral therapies. Nonetheless, its clinical translation has been unaccomplished with the concerns on its exogenous nature and the similarity with human fatty acyl-CoA synthetase. In this study, we aimed to show safety of FLuc by conducting a set of preclinical experiments and a human use. Initially, FLuc permeability across the plasma membrane was investigated by delivering the FLuc-carrying viral vector, OTS-412, or the FLuc recombinant protein. After in vitro infection of OTS-412 into different cancer cell lines, FLuc activity was detected only in the cell lysates, but not in culture media. In addition, recombinant FLuc protein further showed the impermeability against the plasma membrane. Similar result was also observed in the in vivo experiment. After being injected into the VX2 tumor-bearing rabbit, the FLuc exclusively resided within the tumor tissue without being detected in the blood plasma or other organs. Human cancer cell lines originated from various organs were lysed and treated to the FLuc, and none of the human substrates was reactive against the FLuc. As a final step, FLuc recombinant protein was intravenously injected into a human. The luciferase was degraded with the half-life of 20 to 30 minutes in blood, and was untraceable from 1.5 hr after the injection. In addition, the blood plasma was nonresponsive against the fatty acids. Hematological analysis was also comparable between the pre- and post-injection. Altogether, our study collectively demonstrates the safety of the firefly luciferase.

• Journal of Photoscience
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• v.9 no.2
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• pp.482-484
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• 2002

UV irradiation activates various intracellular signaling pathways causing cell death in a DNA damage-dependent and an independent manner. As DNA photoproducts, major forms of DNA damage, are maximally formed by UV light at 260-nm, short wavelength UV (UVC) is more harmful than middle wavelength UV (UVB). However, the differences or similarities in responses of DNA damage-independent intracellular signaling molecules to UVB and UVC are not elucidated. We examined activation of signaling molecules towards apoptosis in normal human fibroblastic cells after irradiation with UVB or UVC at a dose generating the equal amount of DNA photoproducts. Both UVB and UVC induced transient phosphorylation of ERK and sustained phosphorylation of p38. Phosphorylation of p53 at Ser15 and at Ser392 residues were also observed, which were inhibited by a phosphoinositide 3-kinase inhibitor, wortmannin. In contrast, an antioxidant N-acetyl-cysteine and a p38 inhibitor SB203580 suppressed only Ser392 phosphorylation, suggesting that UV-induced oxidative stress and p38 activation were involved in the phosphorylation of this site. The apoptic signals such as mitochondrial cytochrome C release and annexin V binding were then observed. Overall, no difference was found in chronological responses of p53, MAPK, and apoptosis between UVB-irradiated and UVC-irradiated cells. These results suggested that DNA damage-independent intracellular signaling molecules similarly responded to UVB and UVC when the equal level of DNA photoproducts were generated.

• Archives of design research
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• v.20 no.3 s.71
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• pp.247-256
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• 2007

Aging brings color-weakness to the eye due to yellowed eye sight. The 87% of information required for human behavioral decisions are visually perceived. Therefore, the color-weakness will affect behavior in many ways. Most of previous research regarding color planning for the aged focuses on discovering their preferred colors and reflecting the colors on facility color planning. But such research failed to identify the confused range of color cognitions due to the color weakness of the aged. The purpose of this study is to propose a design guide for fee-charging residential facilities for the elderly, reflecting upon color perception characteristics. An experimental simulation on aged vision using the UV filter and Y-2 filter, YA-3 filter alternating for general eyesight, 70 year-old and late 80 year-old was conducted. The results of this study are as follow : color perception is changed by crystalline lens aging process and yellowed eye sight. The recognition arrangement of colors was preferred to contrast harmony rather than similarity harmony and the dominant color is a warm color rather than a cool color.

• Journal of the Korean Society of Costume
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• v.30
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• pp.211-225
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• 1996

In this paper we discuss the corelation be-tween feminism and fashion and examine its influnce on mordern fashion. And we also try to infer what the fashion design will be like in the future by understanding the value of modern fashion from a new viewpoint. The Early Feminism which advocated the similarity between man and women held the thought that women could be equal to men only when they behaved in the same way as men did, In 1970s when the pendulum of the Early Feminism movement reached its highest point a manish look began to flourish. The manish look introduced the items once employed only for men such as trench costs tidy tailored suits shirts and ties to women clothes. It has taken root deeply in women's life and is still employed in various ways by many designers. From the late 1970s the pendulum of feminism movement began to swing toward the Essentialist Feminism which emphasized the differences between men and women. It focused not on the negative image of women seen from male-oriented viewpoints but on their positive image and stressed women's sexual characteristic to make distinction between two genders. in fashion the Essentialist Feminism played a role in bringing about a sexy look which stressed the erotic silhouette of woman's body. The latest feminism is the socio-cultural one. It sees the distinction between men and women as sexual discrimination and introduces the androgynous human which carries the characteristic of two sexes. it leads to ambiguity of sex roles and at the same time serves to make androgynous social atmosphere by admitting the coexistence of two genders. The androgynous idea in fashion is expressed as a new trend which crosses men and women's own beauty while keeping their own identity intact. As we have briefly reviewed feminism in fashion has been presented in various ways with regard to expressing the nature of men and women. And it has continuously indicated the ultimate message of the salvation of mankind such as the respect for humanity and recovery of humanism.

• Archives of design research
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• v.17 no.2
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• pp.189-198
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• 2004

User interlace comes out from the combination of various school systems. It may be a strong point but could be a weak point, as the characteristics and peculiarity of specific school system cannot be well reflected. Therefore, the study aims to find the oughtness of developing the user interface system on which the characteristics and peculiarity of design area are reflected, and to find the necessary features and viewpoint in development. To make this, this study examines the relationship of human and target, and technical form and meaning of the relationship regarding the user interface, and analyzes the similarity and difference through the design comparison. In addition, this study looks the background for the introduction of user interface in design area and analyzes the cause to study the relationship between the design and user interface. Consequently, the concept and system of the user interface can be included in the broad sense of design, but has not been developed into the appropriate system for the characteristics of design. Therefore, it is necessary to develop the concept, method and knowledge considering the characteristics of design upon concept interpretation and development process.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.4
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• pp.1721-1737
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• 2020

Human apparent age estimation has become a popular research topic and attracted great attention in recent years due to its wide applications, such as personal security and law enforcement. To achieve the goal of age estimation, a large number of methods have been pro-posed, where the models derived through the cumulative attribute coding achieve promised performance by preserving the neighbor-similarity of ages. However, these methods afore-mentioned ignore the geometric structure of extracted facial features. Indeed, the geometric structure of data greatly affects the accuracy of prediction. To this end, we propose an age estimation algorithm through joint feature selection and manifold learning paradigms, so-called Feature-selected and Geometry-preserved Least Square Regression (FGLSR). Based on this, our proposed method, compared with the others, not only preserves the geometry structures within facial representations, but also selects the discriminative features. Moreover, a deep learning extension based FGLSR is proposed later, namely Feature selected and Geometry preserved Neural Network (FGNN). Finally, related experiments are conducted on Morph2 and FG-Net datasets for FGLSR and on Morph2 datasets for FGNN. Experimental results testify our method achieve the best performances.

• Journal of Life Science
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• v.14 no.6 s.67
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• pp.986-990
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• 2004

Five strains of Vibrio vulnificus, which cause serious septicemia in human worldwidely, was isolated from marine environments of Korea costal area from May to July of 2002. The isolated strains were identified by API 20E kit and partial 16S rDNA sequence analysis. 16S rDNA sequence of the isolates showed $99\%$ similarity with V. vulnificus ATCC 27562. The proteins of V. vulnificus isolates were examined by analyzing patterns of the cell lysates and outer membrane proteins (OMP). The OMP separated from cell lysates showed the common protein band. Therefore OMP profiles might be useful for the identification of V. vulnificus sp.

• BMB Reports
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• v.33 no.2
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• pp.184-187
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• 2000

Prostaglandin $E_2$ ($PGE_2$) plays an important role in the regulation of various gastric functions, and the growth-inhibitory activities on tumor cells are studied in vitro and in vivo. Although the mechanisms have attracted many researchers in the past decade, the molecular mechanisms of cell cycle arrest, or induction of apoptosis by $PGE_2$, is unclear. We investigated the effects of $PGE_2$ on the growth of the human gastric carcinoma cell line SNU1 and genes that are regulated by $PGE_2$ and isolated them using differential display RT-PCR (DD RT-PCR). FACS analysis suggested that SNU1 cells were arrested at the G1 phase by $PGE_2$ treatment. This growth inhibitory effect was in a time- and dose-dependent manner. Treatment of SNU1 cells with $10\;{\mu}g/ml$ $PGE_2$, followed by DD RT-PCR analysis, revealed differently expressed bands patterns from the control. Among the differently expressed clones, we found an unidentified cDNA clone (HGP-27) overexpressed in $PGE_2$-treated cells. The full-length cDNA of HGP-27 was isolated using RACE, which consisted of a 30-nt 5'-noncoding region, a 891-nt ORF encoding the 296 amino acid protein, and a 738-nt 3'-noncoding region including a poly(a) signal. This gene was localized on the short arm of chromosome number 11. Using the Motif Finder program, a myb-DNA binding repeat signature was detected on the ORF region. The COOH-terminal half was shown to have similarity with the $NH_3$-terminal domain of thioredoxin (Trx). This relation between HGP-27 and Trx implied a potential role for HGP-27 in modulating the DNA binding function of a transcription factor, myb.

• Journal of Intelligence and Information Systems
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• v.13 no.3
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• pp.141-160
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• 2007

User interface has been regarded as a crucial issue to increase the acceptance of mobile services. In special, even though to what extent the machine as speaker communicates with human as listener in a timely and polite manner is important, fundamental studies to come up with these issues have been very rare. Hence, the purpose of this paper is to propose a methodology of estimating politeness level in a certain context-aware setting and then to design a context-aware system for polite mobile interface. We will focus on Korean language for the polite level estimation simply because the polite interface would highly depend on cultural and linguistic characteristics. Nested Minkowski aggregation model, which amends Minkowski aggregation model, is adopted as a privacy-preserving similarity evaluation for case retrieval under distributed computing environment such as ubiquitous computing environment. To show the feasibility of the methodology proposed in this paper, simulation-based experiment with drama cases has performed to show the performance of the methodology proposed in this paper.

• Journal of the Korean Society of Clothing and Textiles
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• v.34 no.8
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• pp.1265-1276
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• 2010

The carelessness of basic design form in education can result in a lack of creativeness and critical thinking ability that will decrease adaptability in a rapidly changing fashion industry. If the fashion education just repeats the previous education of basic form of design, it will be impossible to create original value in fashion design. It is necessary to re-establish the characterized program of the basic form of design education. This study develops concrete educational contents of the basic form of design that can be applied to fashion design through the study of the fundamentals and the concepts of the basic form of design. This study is the basis for the cultivation of talented designers with creativity, forming ability, problem solving skills, and critical thinking ability. The research method is the fundamentals, specialty publications, and designs studied. As a result, an education program for the basic form of design to be used in fashion design education was developed. The development of education contents of the basic form of design has been developed as the plane form that aims the perceptual and emotional effects through the expression of the objects, through the use of dots, lines and planes that are the basics in forming practice and the expression of the abstract images. This suggests subjects composed of the various progressive forming conditions with the abstract dots and lines. In this subject and during the process of the idea, development, and fashion design, the overall unity, harmony, and the theory of gestalt (closure, proximity, and similarity) has been obtained. As a result, the content of the subjects was developed from the perspective that comprehensive decision making skills made from the process can lead to an improved sense of the fashion design.