• Journal of the East Asian Society of Dietary Life
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• v.18 no.6
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• pp.1072-1080
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• 2008

The optimization of the addition ratios of Houttuynia cordata, Saururus chinensis, and Polygonatum odoratum var. pluriflorum hot water extract to Houttuynia cordata beverage was evaluated by applying response surface methodology. The quality characteristics of a selected best product were investigated. Sweet, savory, and fishy taste, and overall acceptability were optimized by response surface methodology using a central composite design. The concentration of effect of Houttuynia cordata, Saururus chinensis, and Polygonatum odoratum var. pluriflorum hot water extract was modeled using an equation. Hot water extracts of Houttuynia cordata ($1.75{\sim}1.89%$), Saururus chinensis ($1.03{\sim}1.04%$), and Polygonatum odoratum var. pluriflorum ($2.17{\sim}2.31%$) represented the optimal concentration conditions to obtain the best taste. The physical and chemical characteristics of the beverage manufactured at optimized conditions were pH 4.18, soluble solids 0.41 $^{\circ}Brix$, total acidity 0.21%, color value ($L^*$ value 40.08, $a^*$ value 4.53, and $b^*$ value 10.69), total polyphenol 54.63 mg/L, and electron donating ability 26.98%.

• Journal of Korean Medicine for Obesity Research
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• v.21 no.1
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• pp.1-9
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• 2021

Objectives: The enzymatic hydrolysis is one of the processing methods that improve its effectiveness on medicinal herbs. In this research, changes in ingredients and activity by enzymatic hydrolysis were studied. Methods: For this study, a carbohydrate hydrolase such as viscozyme, which converts glycosides to aglycone, was applied to induce constituent changes in Sophora japonica Linne, Houttuynia cordata Thunberg and Leonurus japonicus Houttuyn. Changes in antioxidant activity were measured using the 1,1-diphenyl-2-picrylhydrazl (DPPH) method, and changes in ingredients were analyzed by high performance liquid chromatography. Results: As a result of enzymatic hydrolysis, the content of quercetin was increased from 1.26 mg/g to 29.66 mg/g in Sophora japonica Linne, from 0 mg/g to 0.66 mg/g in Houttuynia cordata Thunberg and from 0.43 mg/g to 0.71 mg/g in Leonurus japonicus Houttuyn. As a result of the antioxidant experimentation, the IC₅₀ of Sophora japonica Linne decreased from 5 ug/ml (MeOH extract) and 9.1 ug/ml (EtOAc fraction) to 3.0 ug/ml, Houttuynia cordata Thunberg decreased from 15.6 ug/ml (MeOH extract) and 13.6 ug/ml (EtOAc fraction) to 11.2 ug/ml, and Leonurus japonicus Houttuyn decreased from 14.4 ug/ml (MeOH extract) and 12.6 ug/ml (EtOAc fraction) to 10.2 ug/ml. Conclusion: In conclusion, it was confirmed that glycoside rutin contained in the three medicinal herbs was changed to quercetin which is the aglycone, by the enzymatic hydrolysis using viscozyme. In terms of antioxidant activity, Sophora japonica Linne showed a significant antioxidant activity value that closes to the control group butylated hydroxyanisole. Houttuynia cordata Thunberg and Leonurus japonicus Houttuyn showed a minor increase in antioxidant activity.

• Journal of Life Science
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• v.26 no.4
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• pp.468-474
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• 2016

This study was performed to evaluate the possibility of application of lactic acid bacteria fermentation to increase the anti-oxidative activity of extracts from Houttuynia cordata Thunb. Houttuynia cordata Thunb. was fermented by two species of lactic acid bacteria, Leuconostoc mesenteroides 4395 and Lactobacillus sakei 383. The anti-oxidative activities of Houttuynia cordata Thunb. extracts were analyzed both before and after fermentation. Anti-oxidative activity was determined by in vitro assays to measure 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and super oxide dismutase (SOD)-like activities, and by determining total flavonoid and total phenolic compound contents. The extracts of fermented Houttuynia cordata Thunb. had higher anti-oxidative activity than the unfermented control. The DPPH scavenging activity of the extracts after fermentation by Leuconostoc mesenteroides 4395 at 30℃ for 5 days was 71.67±0.52%, and after Lactobacillus sakei 383 fermentation at 35℃ for 5 days was 70.11±0.67%; these activities were both about 20% higher than the control. Increases of about 10 mg GAE/g of total phenolic compounds were found in both fermented extracts and both contained about 6 mg quercetin equivalents/g of total flavonoids, compared with 35.90±0.61 mg/g and 21.69±1.52 mg/g in the control, respectively. These results also suggested that fermentation time and temperature were important factors in determining the anti-oxidative effect of extracts from fermented Houttuynia cordata Thunb. These findings should be valuable for the development of medicines or functional foods with antioxidative activity.

• Korean Journal of Pharmacognosy
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• v.42 no.2
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• pp.182-186
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• 2011

Houttuynia cordata Thunb.[H.cordata]belonging to Saururaceae, is a wild medicinal herb of perennial plants, and grows well in a place with a lot of shade and moisture. The medical action of H.cordata is reported to have an antitumer effect, toxicity-suppressive effect, antifungal effect, diuretic effect, and antioxidative action, but its effect hasn't been reported on cardiovascular diseases, such as ateriosclerosis and hypertension yet. This study intended to confirm the effect of the water extract of H.cordata on the migration and proliferation of rat aortic smooth muscle cells. Such results show that the water extract of H.cordata suppresses the migration and proliferation of rat aortic smooth muscle cells. It is believed that a useful clue will be offered later to the prevention of cardiovascular diseases such as ateriosclerosis and hypertension, and the development of their medicines on the basis of the fact.

• Toxicological Research
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• v.23 no.1
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• pp.25-31
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• 2007

To evaluate the protective effect of Houttuynia cordata on hydrogen peroxide-induced oxidative DNA damage in HepG2 cell line, we used an alkaline single-cell gel electrophoresis (SCGE; comet assay). The DNA damage was analyzed by tail moment (TM) and tail length (TL), which used markers of DNA strand breaks in SCGE. The $100{\mu}g/ml$ of methanolic extract of Houttuynia cordata root showed significant protective effects (p < 0.01) against hydrogen peroxide-induced DNA damage in HepG2 cells and increased cell viability against hydrogen peroxide. The results of this study indicate that Houttuynia cordata root methanol extract acts as a potential antioxidant, and exhibits potential anticancer properties, which may provide a clue to find applications in new pharmaceuticals for oxidative stability.

• Journal of Environmental Science International
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• v.27 no.5
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• pp.345-348
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• 2018

The influence of various forms of Houttuynia cordata with fermented red koji as feed additives on the performance of duck livestock was evaluated through a field study. Two hundred and forty ducks (Pekins) aged 0-38 d were used. They were randomly assigned to four treatment groups (0%, 1% H. cordata powder with fermented red koji, 1% H. cordata pelleted with fermented red koji, and 1% H. cordata coated with fermented red koji) with four replicates (15 ducks per replication). Foreconomic evaluation, the data (a total of 240 duck) obtained from each treatment were applied to 10,000 ducks per treatment 38-d after the feeding trial. Overall, the results of economic evaluation were the highest in the treatment with 1% H. cordata coated with fermented red koji, followed by 1% H. cordatapelleted with fermented red koji, 1% H. cordatapowder with fermented red koji, and control. In conclusion, using 1% H. cordatawith fermented red koji as coating and pellet type duck diets might be of economic advantage for sustainable environmental management.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.2
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• pp.209-213
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• 1997

Since Houttuynia cordata is well known as a medicinal herb, due to its antibacterial activity on various microorganisms, present investigation was performed to identify the flavor compounds for volatile essential oil. Volatile essential oil was collected by simultaneous distillation-extraction(SDE), and then the oil components were separated on HP-5 capilliary column$(25m{\times}0.25mm\; i.d.)$ and identified those components by GC-MS. Fifty two compounds were isolated from the volatile essential oil of Houttuynia cordata and forty four were positively identified by GC-MS. The volatile compounds were composed mainly of terpenoids(25 classes), aldehydes(7 classes), alcohols(4 classes), ketones(3 classes), acids(1 class) and miscellaneous compounds(4 classes). Of these, the major compounds were ${\beta}-myrcene$, ${\beta}-ocimene$, decanal, 2-undecanone and geranyl propionate.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.17 no.3
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• pp.53-63
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• 2014

This study was carried out to investigate the growth responses of medicinal plants such as Glehnia littoralis, Cryptotaenia japonica, Angelica gigas, Reynoutria japonica, Saururus chinensis, Houttuynia cordata, Ligularia fischeri, and Aralia cordata under 70%-shading and full sunlight conditions on a rooftop condition using artificial soil as a growth media. Glehnia littoralis, Cryptotaenia japonica, Ligularia fischeri, Saururus chinensis, and Houttuynia cordata showed better growth on plant height, leaf length, leaf width, petiole length and leaf number under full sunlight conditions on a rooftop condition. Cryptotaenia japonica and Houttuynia cordata had high value of Hunter's a (red-green) under full sunlight, while had lower value of L(lightness) and b(blue-yellow) than those of 70% shading condition. As an index of plant stress response, Glehnia littoralis, Reynoutria japonica, Houttuynia cordata, Ligularia fischeri showed Fv/Fm values 0.79~0.84 under full sunlight in August, indicating low stress on plant growth. Therefore they seemed to be suitable medicinal plats for rooftop conditions. Ligularia fischeri and Reynoutria japonica showed better growth under 70% shading treatment in August, while showed high growth response under full sunlight conditions in September. The 70% shading treatment was effective for the growth of Angelica gigas, Reynoutria japonica, and Aralia cordata. Angelica gigas and Reynoutria japonica, however, showed better plant growth under full sunlight during summer. The results showed that Glehnia littoralis, Cryptotaenia japonica, Ligularia fischeri, Saururus chinensis, and Houttuynia cordat seemed to be suitable medicinal plants for rooftop garden where there is a full sunlight condition.

• The Korea Journal of Herbology
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• v.28 no.6
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• pp.155-160
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• 2013

Objectives : Gastric cancer is a leading cause of cancer-related deaths, worldwide. Houttuynia cordata Thunberg (H. cordata) has been used as a medicinal plants and it has an anti-cancer activity in human colorectal cancer and leukemic cancer. However, the potential anti-cancer activity and mechanisms of H. cordata for human gastric cancer cells have not been tested so far. Thus, this study examined the biological effects of H. cordata on the human gastric cancer cell line SNU-1 and AGS. Methods : Inhibition of cell proliferation and cell cycle by H. cordata was carried out by MTT assay and Muse cell cycle analysis and the expressions of protein associated with apoptosis and cell cycle regulation were investigated with Western blot analysis. Results : In MTT assay, the proliferation of SNU-1 and AGS cells was significantly inhibited by H. cordata in a time and dose dependent manner, Inhibition of cell proliferation by H. cordata was in part associated with apoptotic cell death, as shown by changes in the expression ratio of Bax to Bcl-2 by H. cordata. Also, H. cordata regulated the expression of cell cycle regulatory proteins such as pRb, cyclin D1, cyclin E, CDK4, CDK2, p21 and p15. Conclusion : The antiproliferative effect of H. cordata on SNU-1 and AGS gastric cancer cells revealed in this study suggests that H. cordata has intriguing potential as a chemopreventive or chemotherapeutic agent.

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.37.1-37.1
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• 2007

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