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http://dx.doi.org/10.5487/TR.2007.23.1.025

Evaluation of Protective Effects of Houttuynia cordata on H2O2-Induced Oxidative DNA Damage Using an Alkaline Comet Assay in Human HepG2 Cells  

Hah, Dae-Sik (Gyengnam Livestock Promotion Institute Middle-branch)
Kim, Chung-Hui (Department of Animal Science and Biotechnology, Jinju National University)
Ryu, Jae-Doo (National Veterinary Research and Quarantine Service Busan Regional Office)
Kim, Eui-Kyung (Department of Pharmacology & Toxicology, College of Veterinary Medicine, Gyeongsang National University)
Kim, Jong-Shu (Department of Pharmacology & Toxicology, College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Toxicological Research / v.23, no.1, 2007 , pp. 25-31 More about this Journal
Abstract
To evaluate the protective effect of Houttuynia cordata on hydrogen peroxide-induced oxidative DNA damage in HepG2 cell line, we used an alkaline single-cell gel electrophoresis (SCGE; comet assay). The DNA damage was analyzed by tail moment (TM) and tail length (TL), which used markers of DNA strand breaks in SCGE. The $100{\mu}g/ml$ of methanolic extract of Houttuynia cordata root showed significant protective effects (p < 0.01) against hydrogen peroxide-induced DNA damage in HepG2 cells and increased cell viability against hydrogen peroxide. The results of this study indicate that Houttuynia cordata root methanol extract acts as a potential antioxidant, and exhibits potential anticancer properties, which may provide a clue to find applications in new pharmaceuticals for oxidative stability.
Keywords
DNA damage; Single cell gel electrophoresis (SCGE); Comet assay; Houttuynia cordota;
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