• Title/Summary/Keyword: Housefly

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Isolation and Purification of Novel Anti-Fungal Peptides from Hemolymph of Immunized Larvae of Housefly, Musca domestica (집파리유충 hemolymph 중신형의 anti-fungal peptides의 분리정제)

  • Gu Li-Juan;Wu Jian-Wei;Su Xiao-Qing;Sung Chang-Keun
    • Journal of Life Science
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    • v.16 no.3 s.76
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    • pp.387-395
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    • 2006
  • To isolate and purify anti-fungal active substances from immunized housefly (Musca domestica), low dose of Candida albicans was injected into the larvae of the housefly to induce the appearance of potent anti-fungal active substances in the hemolymph. This purification work was performed by the routine isolation and purification processes of protein, namely, solid phase extraction (SPE), SDS-PACE electrophoresis, HPLC purification. Three 4-16 kDa peptides which exhibited antifungal activity against Candida albican and other fungi were isolated from induced hemolymph. Consequently, further anti-fungal activity study showed that these three peptides were different either in molecular weight or in anti-fungal activity. All isolated substances were proved to be active and resistant to high-temperature. It was deduced that these peptides isolated from induced housefly were novel members of the insect defensin family and they were inducible.

Effects of several insect growth regulators on the development of housefly, Musca domestica L., larvae (IGR계 살충제가 집파리 유충의 발육에 미치는 영향)

  • Park, Chung-Gyoo
    • The Korean Journal of Pesticide Science
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    • v.2 no.3
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    • pp.137-146
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    • 1998
  • Laboratory tests were carried out to evaluate the effect of several insecticides with insect growth regulator (IGR) properties on the larval development of housefly, Musca domestica, which was collected at a large pigpen in Hamyang, Gyeongnam, Korea in 1997. Commercial formulations of the chemicals were diluted with tap water into a range of concentrations, and mixed with larval media. In addition to the IGRs, imidacloprid 5% WP was tested, too. The IGRs treated at the 2nd instar stage induced higher larval mortalities than percentages of malformed pupae. The result were, however, opposite when the IGRs were treated at the 3rd instar stage. Overall mortality resulting from larval death and malformed pupae was dependent on concentration. Diflubenzuron, teflubenzuron, triflumuron, flufenoxuron, tebufenozide, and imidacloprid, treated to the 2nd instar larvae, showed mortality over 95 % at concentrations of 5 ppm, 3 ppm, 30 ppm, 5 ppm, over 1000 ppm, 1000 ppm, respectively. Higher concentrations were needed to get the same level. of mortality in the 3rd instar larvae as that in the 2nd larvae. Overall mortality over 95% at the 3rd instar could be get at concentrations of 100 ppm, 10 ppm, 300 ppm, 10 ppm of diflubenzuron, teflubenzuron, triflumuron, flufenoxuron, respectively. Tebufenozide (1,000 ppm) and imidacloprid (300 ppm) were less effective than the other chemicals, showing only 36.7% and 86.7% mortalities, respectively. The chemicals also affected pupal weight at high concentrations. Decrease of pupal weight was distinct at high concentrations of teflubenzuron, flufenoxuron, tebufenozide, imidacloprid. Diflubenzuron and triflumuron were less effective. From these results it could be concluded that the IGR insecticides can be used as control agents by interfering with moulting and pupation process of housefly, by reducing pupal weight which could be resulted in low fertility and less oviposition.

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Cloning and Site-Directed Mutagenesis of Musca domestica Acetylcholinesterase for Enhancing Sensitivity to Organophosphorus and Carbamate Insecticides

  • Kim, Chung-Sei;Kim, Su-Il
    • Journal of Microbiology and Biotechnology
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    • v.16 no.11
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    • pp.1760-1772
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    • 2006
  • Mature acetylcholinesterase (AChE) gene (gm, 1,836 bp) was cloned from the housefly and successfully expressed in the E. coli CodonPlus (DE3) RIL system (GM-E, 72 kDa) with a yield of 1,630 mU/g fresh cells. Using the gm, 10 kinds of mutants were constructed and expressed for enhancing sensitivity to insecticides. The sensitivity of these mutants to five kinds of organophosphate (OP) and three carbamate insecticides was investigated by measuring the apparent bimolecular inhibition constant ($k_i=k_2/K_d$). Surprisingly, the sensitivity of quadruple mutant IGFT was enhanced as much as 7-fold for acephate, 164-fold for demeton-S-methyl, 484-fold for dichlorvos, 523-fold for edifenphos, 30-fold for ethoprophos, 30-fold for benfuracarb, 404-fold for carbaryl, and 107-fold for furathiocarb, compared with that of GM-E, although the sensitivity of each single point mutant was slightly increased. These mutational studies indicated that (i) contradictory to Walsh et al. [39], the residue 327 is the important key residue for enhancing sensitivity as much as the residue 262, (ii) the residue 82 and additional residues of 234, 236, and 585 are also important, and (iii) sensitivity was cooperatively accelerated as the number of strategic mutations increased.

Effects of some insect growth regulators on adult and egg viability of the house fly, Musca domestica L. applied via larval medium (유충의 먹이에 처리한 IGR계 살충제가 집파리의 성충과 부화율에 미치는 영향)

  • Park, Chung-Gyoo;Kim, Doo-Ho
    • The Korean Journal of Pesticide Science
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    • v.3 no.1
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    • pp.78-83
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    • 1999
  • Sublethal effects of imidacloprid (30 and 100 ppm) and insect growth regulators; flufenoxuron (3 and 10 ppm), triflumuron (10 ppm), and teflubenzuron (3 ppm), were tested by treatment via larval rearing medium of a housefly, Musca domestica, in laboratory. Pupal weight was significantly reduced by treatment to the 3rd larvae with high concentrations of imidacloprid (100 ppm) and flufenoxuron (10 ppm), and the adults that survived the flufenoxuron 10 ppm treatment deposited significantly fewer eggs compared with controls and other treatments. Adult longevity and egg viability, however, were not affected by any of the treatments.

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Linkage Group Analysis of Profenofos Resistance in the Housefly(Diptera: Mucsidae) (Profenofos에 대한 집파리(Musca domestica L.) 저항성 유전자의 연쇄군 해석)

  • 박정규;정야준부;안용준
    • Korean journal of applied entomology
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    • v.35 no.2
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    • pp.159-163
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    • 1996
  • The profenofos-resistant P-Pro strain of house fly (Muscn domestica L.) was derived from the pyraclofos-resistant strain by selecting with profenofos for 7 generations. The resistance was shown to be incompletely dominant by the reciprocal crosses between the resistant and susceptible strains. Linkage group analysis for the dominant factor responsible for this resistance was carried out by the F, male-backcross method, using susceptible multi-chromosomal marker strain. The major factors for profenofos resistance were located on the second and the fifth chromosome and the other chromosomes had a little effect on the development of this resistance. The male determining factor (M) was linked to the third chromosome in this strain.

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