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http://dx.doi.org/10.5352/JLS.2006.16.3.387

Isolation and Purification of Novel Anti-Fungal Peptides from Hemolymph of Immunized Larvae of Housefly, Musca domestica  

Gu Li-Juan (Gui Yang Medical College)
Wu Jian-Wei (Gui Yang Medical College)
Su Xiao-Qing (Gui Yang Medical College)
Sung Chang-Keun (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University)
Publication Information
Journal of Life Science / v.16, no.3, 2006 , pp. 387-395 More about this Journal
Abstract
To isolate and purify anti-fungal active substances from immunized housefly (Musca domestica), low dose of Candida albicans was injected into the larvae of the housefly to induce the appearance of potent anti-fungal active substances in the hemolymph. This purification work was performed by the routine isolation and purification processes of protein, namely, solid phase extraction (SPE), SDS-PACE electrophoresis, HPLC purification. Three 4-16 kDa peptides which exhibited antifungal activity against Candida albican and other fungi were isolated from induced hemolymph. Consequently, further anti-fungal activity study showed that these three peptides were different either in molecular weight or in anti-fungal activity. All isolated substances were proved to be active and resistant to high-temperature. It was deduced that these peptides isolated from induced housefly were novel members of the insect defensin family and they were inducible.
Keywords
Anti-fungal peptide; Candida albican; hemolymph; housefly larvae; HPLC; SPE;
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