• JSTS:Journal of Semiconductor Technology and Science
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• v.15 no.1
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• pp.48-59
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• 2015

In recent years, there are increasing threats of rootkits that undermine the integrity of a system by manipulating OS kernel. To cope with the rootkits, in Vigilare, the snoop-based monitoring which snoops the memory traffics of the host system was proposed. Although the previous work shows its detection capability and negligible performance loss, the problem is that the proposed design is not acceptable in recent commodity mobile application processors (APs) which have become de facto the standard computing platforms of smart devices. To mend this problem and adopt the idea of snoop-based monitoring in commercial products, in this paper, we propose a snoop-based monitor design called S-Mon, which is designed for the AP platforms. In designing S-Mon, we especially consider two design constraints in the APs which were not addressed in Vigilare; the unified memory model and the crossbar switch interconnect. Taking into account those, we derive a more realistic architecture for the snoop-based monitoring and a new hardware module, called the region controller, is also proposed. In our experiments on a simulation framework modeling a productionquality device, it is shown that our S-Mon can detect the rootkit attacks while the runtime overhead is also negligible.

• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.98-105
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• 1999

Intestinal bacteria comprise one-third of the contents of the large intestine in humans. Their interactions with the gastrointestinal immune system induce characteristic immunological responses which stimulate or suppress the host's defense system. RAW 264.7 murine cell line was used as a macrophage model to assess the effects of the exposure to the isolated human intestinal bacteria, Bacteroides, Bifidobacterium, Eubacterium, Streptococcus, and E. coli, on NO (nitric oxide), $H_2O_2$(hydrogen peroxide), and cytokines IL (interleukin)-6 and TNF (tumor necrosis factor)-a production. RAW 264.7 cells were cultured in the presence of heat-killed bacteria for 24 h at concentrations of 0-$50\mu$g/ml. Our results showed that Bacteroides and E. coli stimulated IL-6, TNF-$\alpha$, NO, and $H_2O_2$production at high levels even at $1\mu$g/ml, whereas Bifidobacterium, Eubacterium, and Streptococcus showed a low level of stimulation at $1\mu$g/ml, and a gradual increase as the cell concentration increased up to $50\mu$g/ml. This result suggests that gram-negative Bacteroides and E. coli are better able to stimulate macrophage than gram-positive Bifidobacterium, Streptococcus, and Eubacterium. The in vitro approaches employed here should be useful in further characterization of the effects of intestinal bacteria on gastrointestinal and systemic immunity.

• The KIPS Transactions:PartC
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• v.11C no.5
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• pp.613-620
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• 2004

Traditionally, simulation method was used to test and evaluate the performance of communication protocol or functional elements for mobile communication service. In this Paper, PCSsim(Personal Communication System Simulator) was realized that can evaluate and review the call process of mobile communication service or to predict its performance by using the object-oriented simulation platform. PCSsim can simulate the base station and mobile host by considering the user's mobility, call generation rate and call duration time. In this paper, based on the simulation, presented the simulation results of hand-off generation ratio according to call generation, user's moving speed and call duration time both in residence area and commercial area, and it was confirmed that the hand-off rates in simulation and actual service environment have similar features. PCSsim can be used in adjusting the characteristics of base station fellowing the dynamic hand-off buffering or the characteristics of user's call in the design stage, and also can be used in building new mobile communication network by reflecting the characteristics of region where the base station is located and the mobility of the user.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38C no.2
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• pp.165-171
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• 2013

WAVE(Wireless Access in Vehicular Environments) is a representative V2V communication protocol and its standards of MAC and PHY parts except for security were published. In order to control traffic flow and ensure driver's safety using V2V communication, various projects are conducting. In particular, safety application has been researched. Therefore, in this paper, we designed the safety application algorithm, which informs a driver of the dangerous status when driver tries to turn left in an intersection and we also implemented the algorithm. Proposed algorithm configures a model for a host vehicle and a vehicle coming in opposite lane and in case that there is collision hazard it provides warning message to driver by using HMI. In order to evaluate the proposed algorithm's performance, we configured the test bed using test vehicles and we tested the algorithm on proving ground with the composed test scenarios. As test results, our system showed excellent performance. If the infrastructures for V2I communications are constructed, we will optimize our system more precisely and stably.

• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1613-1620
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• 2012

Bacterial wilt caused by Ralstonia solanacearum is a devastating disease of many economically important crops. Since there is no promising control strategy for bacterial wilt, phage therapy could be adopted using virulent phages. We used phage PE204 as a model lytic bacteriophage to investigate its biocontrol potential for bacterial wilt on tomato plants. The phage PE204 has a short-tailed icosahedral structure and double-stranded DNA genome similar to that of the members of Podoviridae. PE204 is stable under a wide range of temperature and pH, and is also stable in the presence of the surfactant Silwet L-77. An artificial soil microcosm (ASM) to study phage stability in soil was adopted to investigate phage viability under a controlled system. Whereas phage showed less stability under elevated temperature in the ASM, the presence of host bacteria helped to maintain a stable phage population. Simultaneous treatment of phage PE204 at $10^8$ PFU/ml with R. solanacearum on tomato rhizosphere completely inhibited bacterial wilt occurrence, and amendment of Silwet L-77 at 0.1% to the phage suspension did not impair the disease control activity of PE204. The biocontrol activities of phage PE204 application onto tomato rhizosphere before or after R. solanacearum inoculation were also investigated. Whereas pretreatment with the phage was not effective in the control of bacterial wilt, post-treatment of PE204 delayed bacterial wilt development. Our results suggested that appropriate application of lytic phages to the plant root system with a surfactant such as Silwet L-77 could be used to control the bacterial wilt of crops.

• International Area Studies Review
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• v.15 no.3
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• pp.535-558
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• 2011

Based on the Rodrik's four-way partition of institutions; market creating, market regulatory institution, market stabilizaing and market legitimizing institution, we analyze how FDI and interaction between FDI and democracy affect four kinds of institutions. By using fixed effect and system GMM model we estimate the direct and indirect effect of FDI on institutions within a large panel data set of 186 developing and developed countries for the period 1985-2009. We show that FDI inflows do not have a positive and significant impact on most kinds of institutions while interaction between democracy and FDI inflows have a significant and positive effect on market creating, market legitimizing and market stabilizing institution. The implication is FDI inflow does not directly lead to change the quality of institution but can indirectly improve it on the condition that democracy of host country become mature. To our knowledge this is the first article to empirically test the FDI and four-way unbundled institutions linkages.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.2
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• pp.136-143
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• 2022

Recent progress has been made to establish intestinal organoids for an in vitro model as a potential alternative to an in vivo system in animals. We previously reported a reliable method for the isolation of intestinal crypts from the small intestine and robust three-dimensional (3D) expansion of intestinal organoids (basal-out) in adult bovines. The present study aimed to establish next-generation intestinal organoids for practical applications in disease modeling-based host-pathogen interactions and feed efficiency measurements. In this study, we developed a rapid and convenient method for the efficient generation of intestinal organoids through the modulation of the Wnt signaling pathway and continuous apical-out intestinal organoids. Remarkably, the intestinal epithelium only takes 3-4 days to undergo CHIR (1 µM) treatment as a Wnt activator, which is much shorter than that required for spontaneous differentiation (7 days). Subsequently, we successfully established an apical-out bovine intestinal organoid culture system through suspension culture without Matrigel matrix, indicating an apical-out membrane on the surface. Collectively, these results demonstrate the efficient generation and next-generation of bovine intestinal organoids and will facilitate their potential use for various purposes, such as disease modeling, in the field of animal biotechnology.

• Journal of Life Science
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• v.28 no.11
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• pp.1277-1284
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• 2018

In this study, a repeated yeast integrative plasmid (R-YIp) harboring Cre/loxP system was constructed to integrate various gene expression cassettes into the yeast chromosome. The R-YIp system contains a reusable selective marker (CgTRP1), loxP sequence, and target sequence for integration. Therefore, many gene expression cassettes can be integrated into the same position of the same yeast chromosome. In the present study, several model enzymes involving xylan/xylose metabolism were examined, including endoxylanase (XYLP), ${\beta}$-xylosidase (XYLB), xylose reductase (GRE3) and xylitol dehydrogenase (XYL2). Efficient expression of these genes was obtained using two promoters (GAL10p and ADH1p) and various plasmids (pGMF-GENE and pAMF-GENE plasmids) were constructed. The XYLP, XYLB, GRE3, and XYL2 genes were efficiently expressed under the control of the GAL10 promoter. Subsequently, R-YIps containing the GAL10p-GENE-GAL7t cassette were constructed, resulting in pRS-XylP, pRS-XylB, pRS-Gre3, and pRS-Xyl2 plasmids. These plasmids were sequentially integrated into chromosome VII of a Saccharomyces cerevisiae strain by repeated gene integration and selective marker rescue. These genes were integrated by the R-YIp system and were stably expressed in the yeast transformants to produce active recombinant enzymes. Therefore, we expect that the R-YIp system will be able to overcome current limitations of the host cells and allow selective marker selection for the integration of various genes into the yeast chromosome.

• Bulletin of the Korean Chemical Society
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• v.30 no.12
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• pp.2999-3005
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• 2009

Among the more than 120 different types of human papillomavirus (HPV), types 16 and 18 have been known to be high risk agents that cause cervical cancer. We examined, in an immuno-chromatographic analysis, the potential of using the early gene product, E7 protein, as a diagnostic marker of cervical cancer caused by HPV. We developed monoclonal antibodies specific to HPV-16 and 18 E7 proteins that were produced from bacterial cells using gene recombinant technology. For each E7 protein, the optimal antibody pair was selected using the immuno-chromatographic sandwichtype binding system based on the lateral flow through membrane pores. Under these conditions, this rapid testing assay had a detection capability as low as 2 ng/mL of E7 protein. Furthermore, since viral analysis required the host cell to be lysed using chemicals such as detergents, it was possible that the E7 protein was structurally damaged during this process, which would result in a decrease in detection sensitivity. Therefore, we examined the detrimental effects caused by different detergents on the E7 protein using HeLa cells as the host. In these experiments, we found that the damage caused by the detergent, nonylphenylpolyethylene glycol (NP-40), was minimal relative to Triton X-100 commonly used for the cell lysis. Temperature also affected the stability of the E7 protein, and we found that the E7 protein was stabilized at 4$^{\circ}C$ for about 2 h, which was 4 times longer than at room temperature. Finally, a HPV-infected cervical cancer cell line, which was used as a real sample model, was treated using the optimized conditions and the presence of E7 proteins were analyzed by immuno-chromatography. The results of this experiment demonstrated that this rapid test could specifically detect HPV-infected samples.

• Research in Plant Disease
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• v.23 no.3
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• pp.249-255
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• 2017

The rice blast fungus is a representative model phytopathogenic fungus in which Gene-for-Gene interaction with host rice is applicable. After 1980, eight differential varieties have been constructed and classified to analyze the race of rice blast isolates in Korea. However, since there is limited information about the genetic background of rice blast resistance genes within the Korean differentials, scientific analysis on the emergence of new race or resistance break down was difficult. Recently, a differential system has been developed using monogenic resistance lines to understand the interactions of pathogen race and rice resistance genes. In this study, a total of 50 isolates were selected from four different races isolated in Korea, and they were inoculated into monogenic lines. As a result, the isolates in the same race classified by the Korean differential system reacted differently in single monogenic lines. This suggests that the isolates categorized as the same race group contains different avirulence genes and furthermore, it is presumed that the Korean differential system is difficult to provide useful information for breeding program. For this reason, introduction of differential system using monogenic resistance lines is required in addition to the current system.