Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Effect of Wnt signaling pathway activation on the efficient generation of bovine intestinal organoids

  • Park, Kang Won (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Yang, Hyeon (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Wi, Hayeon (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ock, Sun A (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Poongyeon (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Hwang, In-Sul (Columbia Center for Translational Immunology, Columbia University Irving Medical Center, Columbia University) ;
  • Lee, Bo Ram (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2022.05.03
  • Accepted : 2022.06.08
  • Published : 2022.06.30
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Abstract

Recent progress has been made to establish intestinal organoids for an in vitro model as a potential alternative to an in vivo system in animals. We previously reported a reliable method for the isolation of intestinal crypts from the small intestine and robust three-dimensional (3D) expansion of intestinal organoids (basal-out) in adult bovines. The present study aimed to establish next-generation intestinal organoids for practical applications in disease modeling-based host-pathogen interactions and feed efficiency measurements. In this study, we developed a rapid and convenient method for the efficient generation of intestinal organoids through the modulation of the Wnt signaling pathway and continuous apical-out intestinal organoids. Remarkably, the intestinal epithelium only takes 3-4 days to undergo CHIR (1 µM) treatment as a Wnt activator, which is much shorter than that required for spontaneous differentiation (7 days). Subsequently, we successfully established an apical-out bovine intestinal organoid culture system through suspension culture without Matrigel matrix, indicating an apical-out membrane on the surface. Collectively, these results demonstrate the efficient generation and next-generation of bovine intestinal organoids and will facilitate their potential use for various purposes, such as disease modeling, in the field of animal biotechnology.

Keywords

Acknowledgement

This work was supported by the National Institute of Animal Science (Grant No. PJ01422201) and the collaborative research program between University (2022), Rural Development Administration (RDA), Korea.

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