• Title/Summary/Keyword: Host selection

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Studies on Synonymous Codon and Amino Acid Usage Biases in the Broad-Host Range Bacteriophage KVP40

  • Sau Keya;Gupta Sanjib Kumar;Sau Subrata;Mandal Subhas Chandra;Ghosh Tapash Chandra
    • Journal of Microbiology
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    • v.45 no.1
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    • pp.58-63
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    • 2007
  • In this study, the relative synonymous codon and amino acid usage biases of the broad-host range phage, KVP40, were investigated in an attempt to understand the structure and function of its proteins/protein-coding genes, as well as the role of its tRNAs. Synonymous codons in KVP40 were determined to be AT-rich at the third codon positions, and their variations are dictated principally by both mutational bias and translational selection. Further analysis revealed that the RSCU of KVP40 is distinct from that of its Vibrio hosts, V. cholerae and V. parahaemolyticus. Interestingly, the expression of the putative highly expressed genes of KVP40 appear to be preferentially influenced by the abundant host tRNA species, whereas the tRNAs expressed by KVP40 may be required for the efficient synthesis of all its proteins in a diverse array of hosts. The data generated in this study also revealed that KVP40 proteins are rich in low molecular weight amino acid residues, and that these variations are influenced primarily by hydropathy, mean molecular weight, aromaticity, and cysteine content.

Role of Information Sharing on the Impact of Foreign Banks' Penetration on Banking Competition

  • ZOHREHVAND, Azadeh;IBRAHIM, Saifuzzaman;HABIBULLAH, Muzafar Shah;YUSOP, Zulkornain;MAZLAN, Nur Syazwani
    • The Journal of Asian Finance, Economics and Business
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    • v.7 no.11
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    • pp.707-715
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    • 2020
  • Globalization has led to an increase in foreign banks' penetration. It is argued that the presence of foreign banks may affect the banking sector of the host countries in several ways including their competition level. It is mentioned that the presence of the foreign banks could heightened the level of competition in the banking sector. Nonetheless, the impact of the foreign banks on competition could be influenced by the degree of information sharing in the banking industry. This study investigates the role of information sharing in moderating the impact of foreign bank penetration on host banking sector competition in selected developing countries. We employ panel data samples of 54 developing countries during the period from 1998 to 2016. The estimation is carried out using the two-step system of the Generalized Method of Moments (GMM) regression technique. This technique is adopted due to its robustness to all forms of endogeneity. The findings of this study show that the presence of information sharing could affect the relationship between foreign banks' penetration and competition. They suggest that improvement in information sharing by a host country may help foreign banks to improve monitoring and reduce the moral hazard and adverse selection problem.

Effect of deproteinized bovine bone mineral on cell proliferation in the procedure of guided bone regeneration (골유도재생술시 탈단백 우골이 세포증식에 미치는 영향)

  • In, Young-Mi;Kwon, Young-Hyuk;Park, Joon-Bong;Herr, Yeek
    • Journal of Periodontal and Implant Science
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    • v.34 no.3
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    • pp.683-698
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    • 2004
  • One of the bone substitutes now in routine use, deproteinized bovine bone mineral(DBBM), is regarded as resorbable and osteoconductive, but some studies refute this. The present study was performed to evaluate the effects of DBBM on guided bone regeneration using titanium membrane on the calvaria of rabbit. At 2 weeks, 4 weeks, 8 weeks, and 12 weeks after surgery, the animal was scrificed. Non-decalcified specimens were produced for histologic analysis. The results of this study were as follows : 1. Titanium membrane was biocompatible and capable of space-maintaining, but there was ingrowth of soft tissue through the pore of titanium membrane. 2. There was no resorption or reduction of DBBM with time. 3. Some of the DBBM particles were combined with newly formed bone. But, apart from host bone, a great part of the particles were surrounded by connective tissue. 4. The bone formation was slight vertically and restricted to superficial area of host bone. Whithin the above results, DBBM dose not appear to contribute to bone formation. DBBM may disturb the migration and proliferation of mesenchymal cell derived from host bone and increase the growth of connective tissue. Therefore, careful caution is needed on selection of bone graft material and surgical protocol at guided bone regeneration for implant placement.

The Differences in the Selection of Outward FDI Locations between State- and Privately Owned Enterprises of China: Focusing on the Effects of Host Country Factors (중국 국유기업과 민간기업 간 해외직접투자 입지 차이 분석: 현지국 요인의 영향을 중심으로)

  • Ra, Wonchan;Wu, Mengqiu
    • Korea Trade Review
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    • v.44 no.6
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    • pp.345-361
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    • 2019
  • In recent years, Chinese firms have explosively increased outward foreign direct investment (oFDI). While state-owned enterprises (SOEs) are still dominant in Chinese oFDI, privately-owned enterprises (POEs) are also accelerating their internationalization. These two types of Chinese firms differ in their behavior regarding oFDI. The objective of this paper is to analyze the differences in the choice of oFDI locations between Chinese SOEs and POEs by considering host country factors. By integrating the literature on Chinese firms' oFDI and on FDI locations, we developed six hypotheses concerning how host country factors affect their choice of location. We tested our hypotheses by conducting multiple regression analysis with recent secondary data on 413 Chinese MNEs in 88 countries between 2005 and 2016. The results of the test show that in selecting oFDI locations, Chinese SOEs invest relatively more in countries with richer natural resources, more abundant strategic assets, less production efficiency, higher political risk, and lower institutional quality compared with Chinese POEs. It is our hope that the empirical results of this paper will contribute to research on Chinese oFDI.

Relation between Black Hole Mass and Bulge in Hard X-ray selected Type 1 AGNs

  • Son, Suyeon;Kim, Minjin;Barth, Aaron J.;Ho, Luis C.
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.62.1-62.1
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    • 2020
  • We present a scaling relation between black hole (BH) mass and bulge luminosity for 35 nearby (z<0.1) type 1 active galaxies, selected from the 70-month Swift-BAT X-ray source catalog. Thanks to the unbiased selection and proximity of the parent sample, our sample is suitable to study the physical connection between central black holes and host galaxies. We use the F814W images obtained with the Advanced Camera for Surveys on Hubble Space Telescope, to perform the imaging decomposition with GALFIT. With a careful treatment on the PSF model, we measure the I-band bulge brightness robustly. In combination with the BH mass estimated from a single-epoch spectroscopic data, we present the correlation between BH mass and bulge luminosity of the target AGNs. We demonstrate that our sample marginally lies off from the M(BH)-L(bulge) relation of inactive galaxies. We discuss possible physical origins of this discrepancy. Finally, we present how the relation depends on the photometric properties of AGNs and host galaxies, which may provide an useful insight on the co-evolution between BHs and host galaxies.

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Multivariate Procedure for Variable Selection and Classification of High Dimensional Heterogeneous Data

  • Mehmood, Tahir;Rasheed, Zahid
    • Communications for Statistical Applications and Methods
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    • v.22 no.6
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    • pp.575-587
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    • 2015
  • The development in data collection techniques results in high dimensional data sets, where discrimination is an important and commonly encountered problem that are crucial to resolve when high dimensional data is heterogeneous (non-common variance covariance structure for classes). An example of this is to classify microbial habitat preferences based on codon/bi-codon usage. Habitat preference is important to study for evolutionary genetic relationships and may help industry produce specific enzymes. Most classification procedures assume homogeneity (common variance covariance structure for all classes), which is not guaranteed in most high dimensional data sets. We have introduced regularized elimination in partial least square coupled with QDA (rePLS-QDA) for the parsimonious variable selection and classification of high dimensional heterogeneous data sets based on recently introduced regularized elimination for variable selection in partial least square (rePLS) and heterogeneous classification procedure quadratic discriminant analysis (QDA). A comparison of proposed and existing methods is conducted over the simulated data set; in addition, the proposed procedure is implemented to classify microbial habitat preferences by their codon/bi-codon usage. Five bacterial habitats (Aquatic, Host Associated, Multiple, Specialized and Terrestrial) are modeled. The classification accuracy of each habitat is satisfactory and ranges from 89.1% to 100% on test data. Interesting codon/bi-codons usage, their mutual interactions influential for respective habitat preference are identified. The proposed method also produced results that concurred with known biological characteristics that will help researchers better understand divergence of species.

Development of Host-Vector Systems for Lactic Acid Bacteria (유산균의 Host-Vector System 개발)

  • 윤성식;김창민
    • Microbiology and Biotechnology Letters
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    • v.29 no.1
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    • pp.1-11
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    • 2001
  • Lactic acid bacteria (LAB) are widely used for various food fermentation. With the recent advances in modern biotechnology, a variety of bio-products with the high economic values have been produced using microorganisms. For molecular cloning and expression studies on the gene of interest, E. coli has been widely used mainly because vector systems are fully developed. Most plasmid vectors currently used for E, coli carry antibiotic-resistant markers. As it is generally believed that the antibiotic resistance markers are potentially transferred to other bacteria, application of the plasmid vectors carrying antibiotic resistance genes as selection markers should be avoided, especially for human consump-tion. By contrast, as LAB have some desirable traits such that the they are GRAS(generally recognized as safe), able to secrete gene products out of cell, and their low protease activities, they are regarded as an ideal organism for the genetic manipulation, including cloning and expression of homologous and heterologous genes. However, the vec-tor systems established for LAB are stil insufficient to over-produce gene products, stably, limiting the use of these organisms for industrial applications. For a past decade, the two popular plasmid vectors, pAM$\beta$1 of Streptococcus faecalis and pGK12 theB. subtilis-E. coli shuttle vector derived from pWV01 of Lactococcus lactis ssp. cremoris wg 2, were most widely used to construct efficient chimeric vectors to be stably maintained in many industrial strains of LAB. Currently, non-antibiotic markers such as nisin resistance($Nis^{r}$ ) are explored for selecting recombi-nant clone. In addition, a gene encoding S-layer protein, slp/A, on bacterial cell wall was successfully recombined with the proper LAB vectors LAB vectors for excretion of the heterologous gene product from LAB Many food-grade host vec-tor systems were successfully developed, which allowed stable integration of multiple plasmid copies in the vec-mosome of LAB. More recently, an integration vector system based on the site-specific integration apparatus of temperate lactococcal bacteriophage, containing the integrase gene(int) and phage attachment site(attP), was pub-lished. In conclusion, when various vector system, which are maintain stably and expressed strongly in LAB, are developed, lost of such food products as enzymes, pharmaceuticals, bioactive food ingredients for human consump-tion would be produced at a full scale in LAB.

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Optimized Protocols for Efficient Plant Regeneration and Gene Transfer in Pepper (Capsicum annuum L.)

  • Mihalka, Virag;Fari, Miklos;Szasz, Attila;Balazs, Ervin;Nagy, Istvan
    • Journal of Plant Biotechnology
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    • v.2 no.3
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    • pp.143-149
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    • 2000
  • An Efficient in vitro regeneration system and an optimized Agrobacterium mediated transformation protocol are described, based on the use of young seedling cotyledons of Capsicum annuum L. Optimal regeneration efficiency can be obtained by cultivating cotyledon explants on media containing 4 mg/L benzyladenine and 0.1 mg/L indolacetic acid. The effect of antibiotics used to eliminate Agrobacteria, as well as the toxic level of some generally used selection agents (kanamycin, geneticin, hygromycin, phosphinotricin and methotrexate) in regenerating pepper tissues were determined. To enable the comparison of different selection markers in identical vector background, a set of binary vectors containing the marker genes for NPTII, HPT, DHFR and BAR respectively, as well as the CaMV 35S promoter/enhancer-GUS chimaeric gene was constructed and introduced into four different Agrobacterium host strains.

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Habitat selection in the lesser cuckoo, an avian brood parasite breeding on Jeju Island, Korea

  • Yun, Seongho;Lee, Jin-Won;Yoo, Jeong-Chil
    • Journal of Ecology and Environment
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    • v.44 no.2
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    • pp.106-114
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    • 2020
  • Background: Determining patterns of habitat use is key to understanding of animal ecology. Approximately 1% of bird species use brood parasitism for their breeding strategy, in which they exploit other species' (hosts) parental care by laying eggs in their nests. Brood parasitism may complicate the habitat requirement of brood parasites because they need habitats that support both their host and their own conditions for breeding. Brood parasitism, through changes in reproductive roles of sex or individual, may further diversify habitat use patterns among individuals. However, patterns of habitat use in avian brood parasites have rarely been characterized. In this study, we categorized the habitat preference of a population of brood parasitic lesser cuckoos (Cuculus poliocephalus) breeding on Jeju Island, Korea. By using compositional analyses together with radio-tracking and land cover data, we determined patterns of habitat use and their sexual and diurnal differences. Results: We found that the lesser cuckoo had a relatively large home range and its overall habitat composition (the second-order selection) was similar to those of the study area; open areas such as the field and grassland habitats accounted for 80% of the home range. Nonetheless, their habitat, comprised of 2.54 different habitats per hectare, could be characterized as a mosaic. We also found sexual differences in habitat composition and selection in the core-use area of home ranges (third-order selection). In particular, the forest habitat was preferentially utilized by females, while underutilized by males. However, there was no diurnal change in the pattern of habitat use. Both sexes preferred field habitats at the second-order selection. At the third-order selection, males preferred field habitats followed by grasslands and females preferred grasslands followed by forest habitats. Conclusions: We suggest that the field and grassland habitats represent the two most important areas for the lesser cuckoo on Jeju Island. Nevertheless, this study shows that habitat preference may differ between sexes, likely due to differences in sex roles, sex-based energy demands, and potential sexual conflict.

Pupation Site Selection Behavior of Plodia interpunctella (Lepidoptera: Pyralidae) in Response to Its Parasitoid, Bracon hebetor (Hymenoptera: Braconidae) (보리나방살이고치벌에 대한 행동 반응으로서의 화랑곡나방 유충의 용화장소 선택)

  • Shin, Jae-Hyub;Nam, Young-Woo;Ryoo, Mun-Il
    • Korean journal of applied entomology
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    • v.51 no.3
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    • pp.215-222
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    • 2012
  • Pupation site selection behavior of Plodia interpunctella (H$\ddot{u}$bner) in response to its larval parasitoid, Bracon hebetor Say, was evaluated. Under the presence of the parasitoid, more moth larvae pupated in a layer deeper than 5 mm. Because the parasitoid searched for the host mostly in the surface layer (< 5 mm), larvae in deeper layers were able to successfully escape attack by the parasitoid. However, this behavior did not affect the numerical response of the parasitoid. The parasitoid laid more eggs per host larvae when the number of attacked larvae decreased.