• Research in Plant Disease
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• v.29 no.4
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• pp.363-376
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• 2023

Alternaria black spots or blights in cruciferous crops have been devastating diseases worldwide and led to economic losses in broccoli, Chinese cabbage, kale, radish, rapeseed, etc. These diseases are caused by different Alternaria spp., including A. brassicae, A. brassicicola and A. raphani transmitted from infected seeds or insect vectors. Efforts to excavate disease resistance traits of cruciferous crops against Alternaria black spots or blights have been demonstrated. Genetic resource of disease resistance was investigated in the wild relatives of cruciferous crops, and different cultivars were screened under different inoculation conditions. Development of the disease-resistant lines against Alternaria black spots or blights was also tried via genetic transformation of the cruciferous crops using diverse plant defence-associated genes. Plant immunity activated by pre-treatment with chemicals, i. e. β-amino-n-butyric acid and melatonin, was suggested for reducing Alternaria black spots or blights in cruciferous crops. The disease resistance traits have also been evaluated in model plant Arabidopsis originating from different habitats. Various plant immunity-related mutants showing different disease responses from wild-type Arabidopsis provided valuable information for managing Alternaria black spots or blights in cruciferous crops. In particular, redox regulation and antioxidant responses altered in the Alternaria-infected mutants were discussed in this review.

• 한국약용작물학회:학술대회논문집
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• 2011.09a
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• pp.376-377
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• 2011

• Journal of Bio-Environment Control
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• v.16 no.3
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• pp.222-227
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• 2007

In this study, nutrient solution for Liliaceae (Chinese chive) leafy vegetable was developed. The various strength (1/2, 1 and 3/2) of nutrient solution recommended by National Horticultural Research Institute (NSH) was applied to the crops in deep flow technique (DFT) system for 42 days. The growth of Chinese chive were highest in the treatment of 1/2 strength. The proper constitution of nutrient solution developed for liliaceae crops (NSL) was N 12, P 2.5, K 7, Ca 4 and Mg 2 met. The crops were grown two times in March and September to examine the appropriateness of the NSC. As a result, the relative growth rate and the amount of chlorophyll (SPAD value)of Chinese chive treated with NSL were increased 1.11 times. The most important nutrient factor of ascorbic acid were also increased 1.16 times.

• The Plant Pathology Journal
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• v.17 no.2
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• pp.106-109
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• 2001

A multi-rapid immunofilter paper assay (multi-RIPA) system was prepared for simultaneous diagnosis of three Tobamoviruses, Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), and Zucchini green mottle mosaic virus (ZGMMV) in cucurbitaceous crops. Each of these viruses was specifically detected by the multi-RIPA from cucumber, watermelon, zucchini, and bottle gourd inoculated with the three Tobamoviruses singly or in combination. The three viruses could infect cucumber, watermelon, and bottle gourd ; however, CGMMV could not infect zucchini as the latex-coated CGMMV antibody showed a negative reaction in the multi-RIPA of the virus-infected plant extract. When the minimum detection level of multi-RIPA was compared with that of double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) using CGMMV, the latter was 10 times more sensitive than the former. The detection limit of the multi-RIPA for the purified CGMMV was 50 ng/ml. In a survey of the threeviruses in cucurbits growing in commercial fields in 1999 and 2000, CGMMV was detected in watermelon and cucumber, and ZGMMV was detected only in zucchini growing in plastic houses at the suburbs of Chonju, Korea. However, KGMMV was not found in the commercially growing cucurbit crops in our study, The results suggest that the multi-RIPA can be a simple, rapid, specific and convenient tool to detect simultaneously the Tobamoviruses.

• Korean Journal of Environmental Agriculture
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• v.33 no.1
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• pp.9-16
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• 2014

BACKGROUND: This study was conducted to investigate the effects of seeding timing and method of rye and/or hairy vetch on seasonal soil and foliar nutrient concentrations as well as fruit quality in a pesticide-free pear orchard. METHODS AND RESULTS: The treatments included as followed: single seeding of rye in September (Sep-Mono), November (Nov-Mono), and January (Jan-Mono), or mix seeding of rye+hairy vetch in November (Nov-Mix) and January (Jan-Mix), or sod culture as a control. Cover crops or vegetation was mown and mulched on the soil surface in April and May for two years. Nov-Mix treatment produced the highest dry matter weight of $12,070kg\;ha^{-1}$, with the lowest dry matter weight for sod culture ($6,520kg\;ha^{-1}$), following Jan-Mix ($7,030kg\;ha^{-1}$). Nov-Mix treatments increased potential amount of N, P, and K from the raw materials of the cover crops as well as improved soil physical properties. Nov-Mix treatments overall elevated soil pH, EC, organic matter, and $P_2O_5$ in May compared to other cover crop treatments or sod culture. The difference of the seasonal nutrient concentrations in leaves or fruit qualities were not consistently occurred amongst treatments. CONCLUSION: Nov-Mix treatments showed playing role in a substitute of a chemical fertilizer. Delayed seeding of cover crops such as Jan-Mix did not increase the potential dry matter production due to the short growing period, and the seeding time would affect the dry matter production of cover crops.

• Journal of People, Plants, and Environment
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• v.24 no.2
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• pp.119-135
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• 2021

Background and objective: This study was conducted to identify the components of kindergartener horticultural education by deriving objective components of horticultural education using the Delphi survey method, and then to provide basic data that can be used when creating horticultural programs in the regular curriculum. Methods: A total of 32 experts including professors of early childhood education, kindergarten directors, horticultural therapy professors, and horticultural therapists were selected as the Delphi panel. Of the 32 selected, only 29 answered all three rounds of the surveys. For the first round of the survey, an open-ended questionnaire, was used, and in the second and third rounds closed-ended questionnaires were used. Results: Results indicated that under the category of the goals of horticultural education, there were 7 items related to the current problems of horticultural education, 16 items related to the need for horticultural education in the smart age, 18 items related to the direction of horticultural education, and 5 items related to the areas most suitable for horticulture education for young children in the Nuri Curriculum. Results in the category of the implementation of horticultural education indicated that 2 items related to horticultural education hours, 3 items related to the venue for horticultural education, 2 items related to the activity types applicable to the Nuri Curriculum, and 4 items related to the objects of horticultural activities were derived. As the current problems of horticultural education, the following items were identified: event-oriented activity (M = 4.24) and lack of kindergarten teachers' opportunities for systematic gardening education (M = 4.21). The results related to the necessity of horticultural education indicated the following items: education on respect for life through caring (M = 4.59), emotional intelligence and stability (M = 4.55), directly experience of the growth process of plants (M = 4.55), and development of the five senses (M = 4.55). Finally, within the direction of horticultural education: nurturing the desire to live with nature (M = 4.50), and learning about life (M = 4.44) was identified, which had higher averages. Within the areas of the Nuri Curriculum, which is most consistent with horticultural education, nature exploration (M = 4.69) and the integration of all areas (M = 4.59) were derived as priorities. Also, regarding the implementation of horticultural education, the following items were derived as the priority from the expert group: 30-40 minutes (M = 4.14) and 40-50 minutes (M = 4.14) for class periods, outdoor garden in a kindergarten(M = 4.66) for the venue of gardening education, outside play (M = 4.59) for the activity type, and vegetable crops (M = 4.55) for the objects of gardening activities. Conclusion: It is significant that the goal and implementation of kindergartner horticultural education were objectively derived through collecting opinions of expert panels. Based on the results of this study, a horticultural education program for kindergarten teachers should be implemented.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.295-295
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• 2022

• Horticultural Science & Technology
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• v.35 no.3
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• pp.323-332
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• 2017

Homology-specific transcriptional and post-transcriptional silencing, an intrinsic mechanism of gene regulation in most eukaryotes, can be induced by anti-sense, co-suppression, or hairpin-based double-stranded RNA. Hairpin-based RNA interference (RNAi) has been applied to analyze gene function and genetically modify crops. However, RNAi vector construction usually requires high-cost cloning steps and large amounts of time, or involves methods that are protected by intellectual property rights. We describe a more effective method for generating intron-spliced RNAi constructs. To produce intron-spliced hairpin RNA, an RNAi cassette was ligated with the first intron and splicing sequences of the Brassica rapa ssp. pekinensis histone deacetylase 1 gene. This method requires a single ligation of the PCR-amplified target gene to SpeI-NcoI and SacI-BglII enzyme sites to create a gene-specific silencing construct. We named the resulting binary vector system pKHi and verified its functionality by constructing a vector to silence DIHYDROFLAVONOL 4-REDUCTASE (DFR), transforming it into tobacco plants, and confirming DFR gene-silencing via PCR, RT-qPCR, and analysis of the accumulation of small interfering RNAs. Reduction of anthocyanin biosynthesis was also confirmed by analyzing flower color of the transgenic tobacco plants. This study demonstrates that small interfering RNAs generated through the pKHi vector system can efficiently silence target genes and could be used in developing genetically modified crops.

• Research in Plant Disease
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• v.27 no.3
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• pp.91-98
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• 2021

The current social demand for organic, sustainable, and eco-friendly approaches for farming, while ensuring the health and productivity of crops is increasing rapidly. Biocontrol agents are applied to crops to ensure biological control of plant pathogens. Research on the biological control of white root rot disease caused by a soil-borne pathogen, Rosellinia necatrix, is limited in pears compared to that in apple and avocado. This pathogenic fungus has an extensive host range, and symptoms of this disease include rotting of roots, yellowing and falling of leaves, wilting, and finally tree death. The severity of the disease caused by R. necatrix, makes it the most harmful fungal pathogen infecting the economical fruit tree species, such as pears, and is one of the main limiting factors in pear farming, with devastating effects on plant health and yield. In addition to agronomic and cultural practices, growers use chemical treatments to control the disease. However, rising public concern about environmental pollution and harmful effects of chemicals in humans and animals has facilitated the search for novel and environmentally friendly disease control methods. This review will briefly summarize the current status of biocontrol agents, ecofriendly methods, and possible approaches to control disease in pear orchards.

• Korean Journal of Agricultural Science
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• v.30 no.2
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• pp.128-138
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• 2003

The bacterial density of partially processed horticultural crops in the commercial market was relatively high probably due to improper handing or processing. Between crops, the bacterial density was low in peeled garlic and ranged above $10^8cfu{\cdot}g^{-1}fw$ in other crops. Especially in brake fern, the bacterial density was the highest and non food materials such as packing strips were found. There was difference in the effects of ozone-treated water washing treatment between crops. In root crops such as burdock and lotus root, ozone showed positive effect on preventing discoloration as well as lowing bacterial density resulting in the increase of marketing period. Any positive effect was not found when sliced crops were treated over 5 min at $0.3mg{\cdot}L^{-1}$ of dissolved ozone. In lettuce slices, leaf tissues were severely discolored and rapidly soften at $0.3mg{\cdot}L^{-1}$ for 2 min dipping. Ozone was highly effective on inhibiting bacterium propagation and off-flavor. This effect was more stronger in Fischer ligulariata than brake fern, probably due to the difference of tissue thickness. Results indicated that ozone-treated water washing had a strong potential to improve the market quality of partially processed horticultural crops including dried wild greens and sliced root crops.