• Journal of Embryo Transfer
• /
• v.29 no.3
• /
• pp.289-296
• /
• 2014

The pedigree information and race records of 1,000 m finishing time of Jeju race horses at KRA were used to study the effect of amount of pedigree information and parental misidentification on the estimates of genetic parameters. The modified data sets were made at the range of 2.5 to 25% parental misidentifications or loss of parental information of individuals with an increment of 2.5 percent. For each incremental level, 20 randomly replicated data sets were obtained and analyzed by single-trait analysis with a DF-REML(AI) algorithm. As the rate of misidentification increased or the amount of pedigree information decreased, the estimates of fraction of additive genetics variance component gradually decreased almost linearly (p<0.05), while the estimated fractions of error variance and permanent environmental variance components gradually increased for the finishing time. Regression coefficients of the percentage amount of both parents' information loss and incorrect pedigree information on additive genetic variances were -0.079 and -0.114, respectively (p<0.01). The estimate of heritability decreased by 0.92% for one percent loss of both parents' information and 1.39% for one percent increase of both parental misidentifications of progeny (p<0.01). For the consideration of probable incorrect and missing parent information of progeny in this early population of Jeju horses, the estimates of additive genetic parameters would be biased downward about ten percent. This results indicate that the amount of pedigree information loss and misidentification of progeny would severely affect estimates of genetic parameters and would reduce genetic gains for selection in Jeju horse population.

• Journal of Life Science
• /
• v.32 no.12
• /
• pp.1005-1012
• /
• 2022

This study was conducted to investigate the taxonomic composition and diversity of fecal microbiota between birth and weaning stages of Halla horses using 16S rRNA gene amplicon sequencing analysis. Proteobacteria (35.7%) and Firmicutes (45.6%) were identified as the most common phylum in birth and weaning, respectively. Escherichia (19.7%) and Clostridium (14.0%) were observed as the most dominant genus in birth, and Fibrobacter (6.6%) was the highest in weaning. The results of α-diversity showed that the richness and evenness in microbial communities were statistically significant (p<0.001) in birth and weaning. The results of β-diversity indicated that the birth and weaning stages were clearly divided into two groups at the genus and species levels. Permutational multivariate analysis of variance (PERMANOVA) showed that the microbiota composition differences between birth and weaning were statistically significant (q<0.001). A linear discriminant analysis effect (LEfSe) was performed to select taxonomic makers between the birth and weaning stages. On the genus level, Escherichia, Bacteroides, Clostridium, and Methylobacterium were relatively abundant at birth, whereas Fibrobacter was more abundant at weaning. We expect that this research can be utilized as basic data in the identification of microbial communities involved in disease prevention and nutrient absorption in Halla horses.

• Proceedings of the Korean Society of Veterinary Clinics Conference
• /
• 2009.10a
• /
• pp.257-257
• /
• 2009

• Journal of Animal Science and Technology
• /
• v.60 no.8
• /
• pp.20.1-20.4
• /
• 2018

Platelet rich plasma (PRP) is popularly used in the horse industry to enhance regeneration of tissue injury that has limitation of blood supply. This study aimed to compare the methods for platelet rich plasma preparation since they has not been established yet. Blood was collected from six horses and platelets were concentrated by three different methods (2-step centrifugation, separated centrifugation and separated centrifugation using histopaque). Concentrated blood was analyzed using Advia hematology systems. In the result, separated centrifugation with histopaque showed the significantly lower number of red blood cells than other groups. The 2-step centrifugation showed the significantly higher number of white blood cells than other groups, while it contained the highest concentration of red blood cells among three groups. In the 2-step centrifugation, separated centrifugation and separated centrifugation with histopaque, platelets were concentrated 4.5, 5.3 and 5.6 times, respectively. And no significant difference of the platelet concentration between the three groups was found. This study demonstrated that separated centrifugation using histopaque was the best method for platelet rich plasma preparation because of the proper amount of platelets and the separation of red blood cells from platelet rich plasma.

• Asian-Australasian Journal of Animal Sciences
• /
• v.28 no.11
• /
• pp.1525-1531
• /
• 2015

Using next-generation sequencing, we conducted a genome-wide scan of selective sweeps associated with selection toward genetic improvement in Thoroughbreds. We investigated potential phenotypic consequence of putative candidate loci by candidate gene association mapping for the finishing time in 240 Thoroughbred horses. We found a significant association with the trait for Ral GApase alpha 2 (RALGAP2) that regulates a variety of cellular processes of signal trafficking. Neighboring genes around RALGAP2 included insulinoma-associated 1 (INSM1), pallid (PLDN), and Ras and Rab interactor 2 (RIN2) genes have similar roles in signal trafficking, suggesting that a co-evolving gene cluster located on the chromosome 22 is under strong artificial selection in racehorses.

• Korean Journal of Veterinary Research
• /
• v.29 no.4
• /
• pp.457-460
• /
• 1989

This work was carried out to get some informations about blood types and their researches, involved blood stock and genetic identification. Horses examined were total 55 heads of sire, mare and their progeny in Korean Horse Affairs Association. 1. Albumin phenotypes of 26 mare were examined. The appearance of phenotype AA, BB, AB, was 1, 18, 7 respectively. The gene frequency of albumin A was 0.17 and albumin B was 0.76. 2. The appearance of phenotype AA, BB, AB in 29 progeny was 1, 16, 12 respectively. The gene frequency of albumin A was 0.24 and albumin B was 0.76. The gene frequency of gene A was higher than their parents. 3. Identification of the relationship between parents and their progeny was also examined. 4 of type AB between AA & BB, 4 of type BB between BB & BB, 13 of type AB between BB & AB were borned. In third case, all of progeny was type AB. This results suggest positive relationship between them.

• Korean Journal of Veterinary Research
• /
• v.36 no.1
• /
• pp.51-56
• /
• 1996

Clinical findings of white muscle disease or dystrophia were reported in Hokkaido horses from 1960. This disease was caused by selenium and $\alpha$-tocopherol deficiency. Therefore, this study were conducted to measure serum selenium and $\alpha$-tocopherol levels in Cheju island horses using feedstuffs containing volcanic ashes. The results were as follows : 1. The mean values of serum total selenium and $\alpha$-tocophcrol were $91.90{\pm}49.18ppb$(13.20ppb-193.80ppb) and $0.345{\pm}0.253mg/dl$(0.056mg/dl-1.159mg/dl) in female respectively. There were no significant relation between serum total selenium and $\alpha$-tocopherol levels. 2. 22%(15 of 68) of the horse examined showed selenium levels below 50ppb. 11.8%(8 of 68) showed $\alpha$-tocopherol levels lower than 0.10mg/dl. These results could be useful to diagnose of white muscle disease of Cheju island foals and quality control of feedstuffs of pregnant mares.

• Journal of Veterinary Clinics
• /
• v.14 no.2
• /
• pp.238-243
• /
• 1997

체중이 700-750kg 인 4마리의 중종마에 70분동안 마취를 실시하여 동맥혈의 이 산화탄소 분압과 호기말 가스내의 이산화탄소 분압 사이의 관계를 관찰하였다. 마취도중 자발 호흡, 인공호흡 1(흡기시간 2.0초), 인공호흡 2(흡기시간 2.5초)를 각각 30분,20분,20분씩 실시하였으며 매 10분마다 동맥혈가스분석과 호기말 가스내 이산화탄소분압 측정을 실시하 였다. 동시에 혈압, 심전도, 체온측정을 실시하여 마취된 환축의 상태를 관찰하였다. 2주후에 자세를 달리하여(앙와에서 측와로) 같은 방법으로 재 실험하였다. 호기말 이산화탄소분압은 동맥혈에서보다 평균 10 mmHg 정도 낮은 양상을 보였으나 높은 상관관계를 보였으며 자세에 따른 유의적 차이는 업었다(앙와자세; r=0.949, 측와자세; r=0.920, P<0.01). 이러한 결과를 토 대로 조직에 창상을 줄 수 있는 동맥혈 가스분석 대신 비침습적방법인 호기말 가스내 이산화 탄소 분압을 측정하는 것이 환축의 모니터링에 효과적으로 사용 가능하다는 것을 알 수 있었다.

• Korean Journal of Veterinary Research
• /
• v.14 no.2
• /
• pp.185-189
• /
• 1974

Although considerable research has been done on the blood chemistry of domestic animals, little work has been made of the changes associated with age. Moreover, the records about physiology of the goat were not much available in Korea, and a comprehensive survey of the blood chemical values of the Korean native goat has not been made. The authors intended to biometric study on the hardness of bone of race horse and Jeju horse in Korea. The measurement of hardness of bone were performed in 272 race horses (Thoroughbred 91, Anglo-Arab 107, Arab 74) and in 109 Jeju horses by the caliper by Toryba's method. Some interesting facts obtained through this study were as follows: 1. There was not significant difference of bone hardness between male and female. The average of bone hardness by the Toryba's meteod were $23.07{\pm}1.01$ in race horse and $19.44{\pm}1.84$ in Jeju horse. 2. The grade of bone hardness of race horse were higher than those of Jeju horse(P<0.001). 3. The correlation coefficient between age and grade of bone hardness were r= +0.344 in Jeju horse, theme were statistically significant (P<0.01) and the regression equation was Y=0.29x+18.497.

• Journal of Veterinary Science
• /
• v.19 no.6
• /
• pp.835-839
• /
• 2018

Auditory tube diverticula, also known as guttural pouches, are naturally occurring dilations of the auditory tube in horses that communicate with the nasopharynx through a small ostium. Infection and select other conditions can result in inflammation and narrowing of the nasopharyngeal ostium, which prevents drainage of fluid or egress of air and can lead to persistent infection or guttural pouch tympany. Auditory tube diverticulotomy allows continuous egress from the auditory tube diverticula and is a feature of disease treatment in horses, in which medical treatment alone is not successful. Transpharyngeal endoscopic auditory tube diverticulotomy was performed using a diode laser either at a single dorsal pharyngeal recess location or bilaterally caudal to the nasopharyngeal ostium in 10 horse head specimens. Both methods resulted in clear communication between the nasopharynx and auditory tube diverticula. Diverticulotomy performed in the dorsal pharyngeal recess required less laser energy and activation time and had a shorter surgical duration than diverticulotomy performed caudal to the nasopharyngeal ostium. Further study related to the clinical application of both techniques is warranted.