• Title/Summary/Keyword: Holographic microscopy

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Holographic Microscopy Measurement of Inertia Migration Phenomena of Phytoplankton in Pipe Flows (식물성 플랑크톤의 관유동 내 횡방향 이동현상에 대한 홀로그래픽 실험 연구)

  • Lim, Seung Min;Lee, Sang-Joon
    • Journal of the Korean Society of Visualization
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    • v.10 no.3
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    • pp.11-15
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    • 2012
  • Inertial migration phenomena of phytoplankton in pipe flows were investigated using a digital holography technique. As the Reynolds number increases, the microorganisms suspended in a pipe flow are focused at a certain radial position which is called equilibrium position or pinch point. In this study, the effects of the size of microorganism and Reynolds number in the range of 1 < Re < 78 on the inertial migration were investigated and the results are compared with those for solid particles under similar experimental conditions. As a result, the equilibrium position for the elastic microorganisms is not so distinct, compared to the solid particles. This results from deformation of elastic body shape caused by shear-gradient of surrounding flow.

Auto-focus of Optical Scanning Holographic Microscopy Using Partial Region Analysis (광 스캐닝 홀로그램 현미경에서 부분 영역 해석을 통한 자동 초점)

  • Kim, You-Seok;Kim, Tae-Geun
    • Korean Journal of Optics and Photonics
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    • v.22 no.1
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    • pp.10-15
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    • 2011
  • In this paper, we propose an auto-focusing algorithm which extracts a depth parameter by analyzing a selected part of a hologram, and we use experimental results to show that the algorithm is practical. First, we record a complex hologram using Optical Scanning Holography. Next we select some part of hologram and extract depth information through Gaussian low pass filtering, synthesizing a real-only hologram, power fringe-adjusted filtering and inverting to a new frequency axis. Finally, we reconstruct the hologram automatically using the extracted depth location.

Design of Dynamically Focus-switchable Fresnel Zone Plates Based on Plasmonic Phase-change VO2 Metafilm Absorbers

  • Kyuho Kim;Changhyun Kim;Sun-Je Kim;Byoungho Lee
    • Current Optics and Photonics
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    • v.7 no.3
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    • pp.254-262
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    • 2023
  • Novel thermo-optically focus-switchable Fresnel zone plates based on phase-change metafilms are designed and analyzed at a visible wavelength (660 nm). By virtue of the large thermo-optic response of vanadium dioxide (VO2) thin film, a phase-change material, four different plasmonic phase-change absorbers are numerically designed as actively tunable Gires-Tournois Al-VO2 metafilms in two and three dimensions. The designed phase-change metafilm unit cells are used as the building blocks of actively focus-switchable Fresnel zone plates with strong focus switching contrast (40%, 83%) and high numerical apertures (1.52, 1.70). The Fresnel zone plates designed in two and three dimensions work as cylindrical and spherical lenses in reflection type, respectively. The coupling between the thermo-optic effect of VO2 and localized plasmonic resonances in the Al nanostructures offer a large degree of freedom in design and high-contrast focus-switching performance based on largely tunable absorption resonances. The proposed method may have great potential in photothermal and electrothermal active optical devices for nonlinear optics, microscopy, 3D scanning, optical trapping, and holographic displays over a wide spectral range including the visible and infrared regimes.

Measurement of Width and Step-Height of Photolithographic Product Patterns by Using Digital Holography (디지털 홀로그래피를 이용한 포토리소그래피 공정 제품 패터닝의 폭과 단차 측정)

  • Shin, Ju Yeop;Kang, Sung Hoon;Ma, Hye Joon;Kwon, Ik Hwan;Yang, Seung Pil;Jung, Hyun Chul;Hong, Chung Ki;Kim, Kyeong Suk
    • Journal of the Korean Society for Nondestructive Testing
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    • v.36 no.1
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    • pp.18-26
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    • 2016
  • The semiconductor industry is one of the key industries of Korea, which has continued growing at a steady annual growth rate. Important technology for the semiconductor industry is high integration of devices. This is to increase the memory capacity for unit area, of which key is photolithography. The photolithography refers to a technique for printing the shadow of light lit on the mask surface on to wafer, which is the most important process in a semiconductor manufacturing process. In this study, the width and step-height of wafers patterned through this process were measured to ensure uniformity. The widths and inter-plate heights of the specimens patterned using photolithography were measured using transmissive digital holography. A transmissive digital holographic interferometer was configured, and nine arbitrary points were set on the specimens as measured points. The measurement of each point was compared with the measurements performed using a commercial device called scanning electron microscope (SEM) and Alpha Step. Transmission digital holography requires a short measurement time, which is an advantage compared to other techniques. Furthermore, it uses magnification lenses, allowing the flexibility of changing between high and low magnifications. The test results confirmed that transmissive digital holography is a useful technique for measuring patterns printed using photolithography.

Compression Performance Comparison of Fringe Pattern and Phase Data for DHM (DHM을 위한 간섭무늬 압축 방법과 위상 압축 방법의 성능 비교)

  • YoungMin Kim;Hyunmin Ban;Heeyeon Koo;SeungMi Choi;Kwan-Jung Oh;Yongjun Lim;Hui Yong Kim
    • Journal of Broadcast Engineering
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    • v.28 no.1
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    • pp.55-78
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    • 2023
  • The fringe pattern obtained through a DHM (Digital Holographic Microscopy) contains the thickness information of the sample. However, there is a disadvantage that the data capacity is large. Therefore, a compression method that can reduce the data size while minimizing damage to the thickness information of the sample contained in the fringe pattern is required. This paper presents the phase compression method and confirmed through experiments that the phase compression method is more efficient that the fringe pattern compression method used in JPEG Pleno Holography. As a result of evaluation using RMSE, BD-Rate and PSNR the phase compression method showed up to 92.39% improvement in performance than the fringe pattern compression method. In addition, experiment were conducted under various conditions to compare and analyze the compression performance for each condition. In the case of the fringe pattern compression method, it includes not only the phase information for calculating the thickness of the sample but also other information, whereas the phase compression method compresses only the phase information after removing unnecessary information from the fringe pattern. It is judged to have high performance.