Korean Journal of Optics and Photonics (한국광학회지)

Optical Society of Korea (한국광학회)
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Auto-focus of Optical Scanning Holographic Microscopy Using Partial Region Analysis

광 스캐닝 홀로그램 현미경에서 부분 영역 해석을 통한 자동 초점

  • Kim, You-Seok (Department of Optical Engineering, Sejong University) ;
  • Kim, Tae-Geun (Department of Optical Engineering, Sejong University)
  • Received : 2010.10.01
  • Accepted : 2011.02.14
  • Published : 2011.02.25
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Abstract

In this paper, we propose an auto-focusing algorithm which extracts a depth parameter by analyzing a selected part of a hologram, and we use experimental results to show that the algorithm is practical. First, we record a complex hologram using Optical Scanning Holography. Next we select some part of hologram and extract depth information through Gaussian low pass filtering, synthesizing a real-only hologram, power fringe-adjusted filtering and inverting to a new frequency axis. Finally, we reconstruct the hologram automatically using the extracted depth location.

본 논문에서 홀로그램의 일부 영역만을 선택하여 자동 초점 맺는 방법을 제안하고 이를 실험적으로 보였다. 먼저 광 스캐닝 홀로그래피를 이용하여 복소 홀로그램을 추출한다. 그 다음 홀로그램의 일부 영역만을 선택하고 가우시안 저대역 필터링, Real-only 홀로그램 합성, Power fringe-adjusted 필터링, 주파수축 변환 등의 과정을 통해 깊이 정보를 추출한다. 이렇게 추출된 깊이 위치를 이용하여 홀로그램을 자동적으로 복원하게 된다.

Keywords

References

  1. D. Gabor, “A new microscope principle,” Nature 161, 777-778 (1948).
  2. G. Indebetouw, T. Kim, T.-C. Poon, and B. W. Schilling, “Three-dimensional location of fluorescent inhomogeneities in turbid media by scanning heterodyne holography,” Opt. Lett. 23, 135-137 (1998). https://doi.org/10.1364/OL.23.000135
  3. J. Rosen and G. Brooker, “Non-scanning motionless fluorescence three-dimensional holographic microscopy,” Nat. Ph. 2, 190-195 (2008). https://doi.org/10.1038/nphoton.2007.300
  4. T. Kim, “Optical sectioning by use of optical scanning holography and wiener filter,” Appl. Opt. 45, 872-879 (2006). https://doi.org/10.1364/AO.45.000872
  5. T. Kim and T.-C. Poon “Autofocusing in optical scanning holography,” Appl. Opt. 48, H153-H159 (2009). https://doi.org/10.1364/AO.48.00H153
  6. T. Kim and Y.-S. Kim, “Extraction of a distance parameter in optical scanning holography using axis transformation,” J. Opt. Soc. Korea 14, 104-108 (2010). https://doi.org/10.3807/JOSK.2010.14.2.104
  7. P. Ferraro, G. Coppola, S. D. Nicola, A. Finizio, and G. Pierattini, “Digital holographic microscope with automatic focus tracing by detecting sample displacement in real time,” Opt. Lett. 28, 1257-1259 (2003). https://doi.org/10.1364/OL.28.001257
  8. M. Liebling and M. Unser, “Autofocus for digital Fresnel holograms by use of a Fresnelet-sparsity criterion,” J. Opt. Soc. Am. A 21, 2424-2430 (2004). https://doi.org/10.1364/JOSAA.21.002424
  9. P. Langehanenberg, B. Kemper, D. Dirksen, and G. von Bally, “Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging,” Appl. Opt. 47, D176 (2008). https://doi.org/10.1364/AO.47.00D176
  10. T.-C. Poon, M. Wu, K. Shinoda, and Y. Suzuki, “Optical scanning holography,” Proc. IEEE 84, 753-764 (1996). https://doi.org/10.1109/5.488744
  11. G. Indebetouw, P. Klysubun, T. Kim, and T.-C. Poon, “Imaging properties of scanning holographic microscopy,” J. Opt. Soc. Am. A 17, 380-390 (2000). https://doi.org/10.1364/JOSAA.17.000380
  12. P. Klysubun, G. Indebetouw, T. Kim, and T.-C. Poon, “Accuracy of three-dimensional remote target location using scanning holographic correlation,” Opt. Comm. 184, 357-366 (2000). https://doi.org/10.1016/S0030-4018(00)00969-X
  13. T. Kim and T.-C Poon, “Extraction of 3-D location of matched 3-D object using power fringe-adjusted filtering and Wigner analysis,” Opt. Eng. 38, 2176-2183 (1999). https://doi.org/10.1117/1.602326
  14. T. Kim and T.-C. Poon, “Experiments of depth detection and image recovery of a remote target using a complex hologram,” Opt. Eng. 43, 1851-1855 (2004). https://doi.org/10.1117/1.1764574
  15. T. Kim, T.-C. Poon, and G. Indebetouw, “Depth detection and image recovery in remote sensing by optical scanning holography,” Opt. Eng. 41, 1331-1338 (2002). https://doi.org/10.1117/1.1476939
  16. T. Kim, “Optical sectioning by use of optical scanning holography and Wiener filter,” Appl. Opt. 45, 872-879 (2006). https://doi.org/10.1364/AO.45.000872
  17. T. Kim, Y. S. Kim, W. S. Kim, and T.-C. Poon, “Algorithm for converting full-parallax holograms to horizontal-parallaxonly holograms,” Opt. Lett. 34, 1231-1233 (2009).