• The Korean Journal of Mycology
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• v.24 no.1 s.76
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• pp.67-80
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• 1996

Eleven fruit bodies of Ganoderma sp. were collected from eight locations throughout the forest of Kangwon province and Kyunggi province in Korea. The hosts in forest were cut trunks of Quercus dentata, Q. variabilis, Prunus peria and Alnus japonica that was newly surveyed but 5 isolates were collected at the farms of Ganoderma mushroom. Most fruit bodies were formed solitarily on the cut trunks but GS-106 isolate grown in crowds on cut trunk of Alnus japonica. Optimal temperature ranges for isolates of species studied were: G. applanatum $28^{\circ}C{\sim}30^{\circ}C$, G. lucidum $28{\sim}30^{\circ}C$, G. neo-japonicum $28^{\circ}C$, and G. tsuage $26^{\circ}C$ and all the species grew slowly at the $32^{\circ}C$. Hamada medium adjusted with pH 5.4 and 6.2 is better than other media for mycelial growth. Mycelial morphological characteristics of six species were studied: G. applanatum, G. lucidum and G. neo-japonicum produced typical type of staghoru hyphae but G. oregonens and G. valeosiacum produced staghoru hyphae with a branch of grape form. Clamp connection was observed on hypha of G. applanatum, G. lucidum, G. oregonense and G. valeosiacum except G. neo-japonicum with node type. Chlamydospore was produced by G. applanatum, G. neo-japonicum. and cuticular cells were present on hyphae of G. lucidum, G. neo-japonicum, G.oregonense and G. tsugae.

• Journal of Oral Medicine and Pain
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• v.34 no.3
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• pp.303-316
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• 2009

This study was designed to evaluate the Temporomandibular Disorders(TMD) with Tension-Type Headache(TTH) by gender. Patients with TMD and/or TTH visited the Department of Oral Medicine, Kyung Hee University Dental Hospital were recruited to this study. Experimental group(n=60) is composed of TMD with TTH and control group(n=111) is composed of TMD without TTH. Evaluation list was pain quality, pain intensity, pain laterality, pain increase by routine physical activity and then it was analyzed statistically. The results were as follows ; 1. In the control group, pain quality was significantly different by gender(p=0.04). But, in the experimental group, pain quality was not significantly different by gender. 2. In the control group, pain intensity was not significantly different by gender. And, in the experimental group, pain intensity was not significantly different by gender. 3. In the control group, pain laterality was not significantly different by gender. And, in the experimental group, pain laterality was not significantly different by gender. 4. In the control group, pain increase by routine physical activity was not significantly different by gender. And, in the experimental group, pain increase by routine physical activity was not significantly different by gender. Therefore, it is considered that not temporomandibular disorder patients with tension-type headache but temporomandibular disorder patients without tension-type headache was influenced by gender in the pain quality.

• Journal of the Korean Ceramic Society
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• v.45 no.11
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• pp.701-706
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• 2008

High frequency dielectric ceramics have potential for applications in mobile and satellite communications systems at frequencies higher than 10GHz. The Ba$(Zn_{1/3}Ta_{2/3})O_3$ ceramics are known to have a high quality factor, a small temperature coefficient of the resonance frequency and a high dielectric constant. On the other hands, sintering at high temperature for extended time is required to obtain the ordered structure for high quality factor. In this study, the microwave dielectric properties of $ZrO_2$ and NiO-added Ba$(Zn_{1/3}Ta_{2/3})O_3$ ceramics prepared by solid-state reaction have been investigated. Adding $ZrO_2$ and NiO could effectively promote the densification even the case of decreasing the sintering time. At the surface of samples, secondary phase of Ba-Ta compounds was formed possibly due to the evaporation of ZnO, however, the interior of the samples remained as pure Ba$(Zn_{1/3}Ta_{2/3})O_3$. The samples sintered at $1600^{\circ}C$ for 2h exhibited 1:2 ordering of Zn and Ta cations. Excellent microwave dielectric properties of $Q{\cdot}f$(>96,000 GHz) and ${\varepsilon}_r$=30 has been obtained.

• The Korea Journal of Herbology
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• v.31 no.5
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• pp.71-78
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• 2016

Objectives : Diabetic nephropathy is one of the most common chronic complications of diabetes and a leading cause of end-stage renal failure in the world. Mesangial cell proliferation is known as the major pathologic features such as glomerulosclerosis and renal fibrosis. Wiryeongtang (WRT) is a well-known traditional herbal formula as therapeutic agents for chronic edema and dysuresia of renal homeostasis. In the present study, we investigated whether WRT inhibits high glucose (HG)-induced renal dysfunction by TGF-β/Smads signal regulation in cultured mesangial cells.Methods : Inhibitory effect of WRT (10-50 ㎍/ml) on HG-stimulated mesangial cells proliferation and dysfunction were evaluated by [³H]-thymidine incorporation, Western blot, and RT-qPCR.Results : WRT significantly decreased HG-accelerated thymidine incorporation in human renal mesangial cell in a dose-dependent levels. WRT induced down-regulation of cyclins/CDKs and up-regulation of CDK inhibitor, p21waf1/cip1 and p27kip1 expression. In addition, HG enhanced expression of dysfunction biomarker such as collagen IV and CTGF, which was markedly attenuated by WRT. WRT decreased TGF-β1 and Smad-2/Smad-4 expression, whereas increased Smad-7 expression under HG. Furthermore, WRT inhibited HG-induced inflammatory factors level such as ICAM-1 and MCP-1 as well as NF-κB p65 nuclear translocation and intracellular ROS production.Conclusions : These results suggested that WRT may alleviate mesangial proliferation and inflammation possibly involved in renal fibrotic process, further diabetic nephropathy through disturbing TGF-β1/Smad signaling and NF-κB/ROS pathway. Thus, WRT might prove to be effective in the treatment of renal dysfunction leading to diabetic nephropathy.

• Korean Journal of Environmental Agriculture
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• v.26 no.2
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• pp.149-158
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• 2007

The objective of this study were to offer a basis data for application of management of forest resources through grasping of species composition by layer of forest vegetation in the Mt. Geumwon recreational forest. The tree and subtree layer consisted of 27 and 44 species in Mt. Geumwon recreational forest respectively. The importance value of Quercus mongolica (57.1) and Q. serrata (40.4) were highest in the tree layer and that of Acer pseudo-sieboldianum (43.4) was highest in the subtree layer, respectively. The shrub and herb layer was composed of 71 and 69 species, respectively. The value of species diversity ranged from 1.0391 to 1.5403. Evenness ranged from 0.7259 to 0.8320. Dominance was 0.2741 at tree layer, 0.2440 at subtree layer, 0.1680 at shrub layer, and 0.1702 at herb layer, which showed that Mt. Geumwon recreational forest is dominated by a large number of species at all layer. The result of forest vegetation in Mt. Geumwon recreational forest may be utilized as a basic data for application of reasonable ecological management of forest resources.

• The Korean Journal of Pesticide Science
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• v.7 no.2
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• pp.108-116
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• 2003

A series of new quinclorac family, herbicidal 3-phenyl-5-(3,7-dichloro-8-quinolinyl)-1,2,4-oxadiazole derivatives as substrate were synthesized and their growth inhibition activity $(pI_{50})$ against root and shoot of rice plant (Oryza sativa L.) and barnyard grass (Echinochloa crus-galli) were determined. And then comparative molecular field analysis (CoMFA) and molecular holographic quantitative structure- activity relationship (HQSAR) were compared in terms of their potential for predictiability. The statistical results were suggested that HQSAR based model had better predictability than CoMFA model. The selective factors to remove barnyard grass take electron withdrawing groups which can be created positive charge and steric bulky on the phenyl ring. Results revealed that the unknown 2,6-dichloro-substituent, U5 and 2,4,6-trichloro-substituent, U6(${\Delta}pI_{50}$=CoMFA: 1.18 & HQSAR: 1.82) were predicted as compound with higher activity and selectivity.

• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.444-450
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• 2013

A Gram-negative, strictly aerobic, non-motile, non-spore-forming, and rod-shaped bacterial strain designated FW-$6^T$ was isolated from a freshwater sample and its taxonomic position was investigated by using a polyphasic approach. Strain FW-$6^T$ grew optimally at $10-42^{\circ}C$ and at pH 7.0 on nutrient and R2A agar. Strain FW-$6^T$ displayed ${\beta}$-glucosidase activity that was responsible for its ability to transform ginsenoside $Rb_1$ (one of the dominant active components of ginseng) to Rd. On the basis of 16S rRNA gene sequence similarity, strain FW-$6^T$ was shown to belong to the family Sphingomonadaceae and was related to Novosphingobium aromaticivorans DSM $12444^T$ (98.1% sequence similarity) and N. subterraneum IFO $16086^T$ (98.0%). The G+C content of the genomic DNA was 64.4%. The major menaquinone was Q-10 and the major fatty acids were summed feature 7 (comprising $C_{18:1}{\omega}9c/{\omega}12t/{\omega}7c$), summed feature 4 (comprising $C_{16:1}{\omega}7c/iso-C_{15:0}2OH$), $C_{16:0}$, and $C_{14:0}$ 2OH. DNA and chemotaxonomic data supported the affiliation of strain FW-$6^T$ to the genus Novosphingobium. Strain FW-$6^T$ could be differentiated genotypically and phenotypically from the recognized species of the genus Novosphingobium. The isolate that has ginsenoside converting ability therefore represents a novel species, for which the name Novosphingobium ginsenosidimutans sp. nov. is proposed, with the type strain FW-$6^T$ (= KACC $16615^T$ = JCM $18202^T$).

• Geophysics and Geophysical Exploration
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• v.9 no.3
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• pp.241-249
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• 2006

Due to the long tectonic history and the very complex geologic formations in Korea, the anisotropic characteristics of subsurface material may often change very greatly and locally. The algorithms commonly used, however, may not give sufficiently precise computational results of traveltime data particularly for the complex and strong anisotropic model, since they are based on the two-dimensional (2D) earth and/or weak anisotropy assumptions. This study is intended to develope a three-dimensional (3D) modeling algorithm to precisely calculate the first arrival time in the complex anisotropic media. Considering the complex geology of Korea, we assume 3D TTI (tilted transversely isotropy) medium having the arbitrary symmetry axis. The algorithm includes the 2D non-linear interpolation scheme to calculate the traveltimes inside the grid and the 3D traveltime mapping to fill the 3D model with first arrival times. The weak anisotropy assumption, moreover, can be overcome through devising a numerical approach of the steepest descent method in the calculation of minimum traveltime, instead of using approximate solution. The performance of the algorithm developed in this study is demonstrated by the comparison of the analytic and numerical solutions for the homogeneous anisotropic earth as well as through the numerical experiment for the two layer model whose anisotropic properties are greatly different each other. We expect that the developed modeling algorithm can be used in the development of processing and inversion schemes of seismic data acquired in strongly anisotropic environment, such as migration, velocity analysis, cross-well tomography and so on.

• KSBB Journal
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• v.22 no.2
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• pp.109-113
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• 2007

Suspension culture of recombinant Chinese hamster ovary (CHO) cells producing follicle-stimulating hormone was performed to investigate the effect of glycine betaine on cell growth and FSH production at low culture temperature. At 28$^{\circ}C$, cell growth was suppressed, but cell viability remained high for a longer culture period. When the culture temperature was lowered from 37$^{\circ}C$ to 28$^{\circ}C$, more than 14-fold increase in the maximum FSH titer was achieved. In batch culture at 28$^{\circ}C$, the use of 15 mM glycine betaine (GB) to culture medium resulted in the enhancement of maximum cell density and FSH titer by 11% and 17%, respectively, compared to the culture without GB. In pseudo-perfusion culture at 28$^{\circ}C$ with the exchange of fresh medium containing 15 mM GB, a final FSH of $2,058{\mu}g$ which is approximately 1.4-fold higher as compared to the culture without GB was obtained. This enhanced FSH production with 15 mM GB was not just because of enhanced specific FSH productivity (qFSH), but mainly because of the extended culture longevity. Taken together, this result demonstrates that the application of GB at low culture temperature is feasible to enhance the production of recombinant proteins in rCHO cells.

• Korean Journal of Environment and Ecology
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• v.25 no.4
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• pp.573-580
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• 2011

Litter production, nutrient concentration of each component of litterfall and amount of nutrients input to forest floor via litterfall were investigated for four years from May 2005 through April 2009 in Quercus variabilis forest at Mt. Worak National Park. Amount of litterfall in 2005, 2006, 2007, 2008 were 5.181, 5.372, 5.314, 5.361 ton $ha^{-1}yr^{-1}$, respectively. Average amount of litterfall for four years was 5.307${\pm}$0.09 ton $ha^{-1}yr^{-1}$. Average percentage of leaf litter, branch and bark, reproductive organ and the miscellaneous for four years were 67.5${\pm}$1.9, 13.3${\pm}$0.8, 10.1${\pm}$0.5 and 9.1${\pm}$1.2%, respectively. Average amount of N, P, K, Ca and Mg returned to forest floor via litterfall for four years in this Q. variabilis forest were 44.47${\pm}$6.70, 2.50${\pm}$0.25, 12.26${\pm}$0.30, 17.23${\pm}$2.32 and 9.56${\pm}$0.54 kg $ha^{-1}yr^{-1}$, respectively.