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http://dx.doi.org/10.6116/kjh.2016.31.5.71.

Wiryeongtang attenuates diabetic renal dysfunction in human renal mesangial cells  

Yoon, Jung Joo (College of Korean Medicine, Wonkwang University)
Han, Byung Hyuk (College of Korean Medicine, Wonkwang University)
Choi, Eun Sik (Hanbang Body-fluid Research Center, Wonkwang University)
NamGung, Seung (College of Korean Medicine, Wonkwang University)
Jeong, Da Hye (College of Korean Medicine, Wonkwang University)
Kim, Hye Yoom (College of Korean Medicine, Wonkwang University)
Ahn, You Mee (College of Korean Medicine, Wonkwang University)
Lee, Yun Jung (College of Korean Medicine, Wonkwang University)
Kang, Dae Gill (College of Korean Medicine, Wonkwang University)
Lee, Ho Sub (College of Korean Medicine, Wonkwang University)
Publication Information
The Korea Journal of Herbology / v.31, no.5, 2016 , pp. 71-78 More about this Journal
Abstract
Objectives : Diabetic nephropathy is one of the most common chronic complications of diabetes and a leading cause of end-stage renal failure in the world. Mesangial cell proliferation is known as the major pathologic features such as glomerulosclerosis and renal fibrosis. Wiryeongtang (WRT) is a well-known traditional herbal formula as therapeutic agents for chronic edema and dysuresia of renal homeostasis. In the present study, we investigated whether WRT inhibits high glucose (HG)-induced renal dysfunction by TGF-β/Smads signal regulation in cultured mesangial cells.Methods : Inhibitory effect of WRT (10-50 ㎍/ml) on HG-stimulated mesangial cells proliferation and dysfunction were evaluated by [3H]-thymidine incorporation, Western blot, and RT-qPCR.Results : WRT significantly decreased HG-accelerated thymidine incorporation in human renal mesangial cell in a dose-dependent levels. WRT induced down-regulation of cyclins/CDKs and up-regulation of CDK inhibitor, p21waf1/cip1 and p27kip1 expression. In addition, HG enhanced expression of dysfunction biomarker such as collagen IV and CTGF, which was markedly attenuated by WRT. WRT decreased TGF-β1 and Smad-2/Smad-4 expression, whereas increased Smad-7 expression under HG. Furthermore, WRT inhibited HG-induced inflammatory factors level such as ICAM-1 and MCP-1 as well as NF-κB p65 nuclear translocation and intracellular ROS production.Conclusions : These results suggested that WRT may alleviate mesangial proliferation and inflammation possibly involved in renal fibrotic process, further diabetic nephropathy through disturbing TGF-β1/Smad signaling and NF-κB/ROS pathway. Thus, WRT might prove to be effective in the treatment of renal dysfunction leading to diabetic nephropathy.
Keywords
Wiryeongtang; diabetic nephropathy; mesangial cell; TGF-β1; renal dysfunction;
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